Amino acid dipepetide frequency for Streptomyces phage RosaAsantewaa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.678AlaAla: 9.678 ± 2.397
0.372AlaCys: 0.372 ± 0.173
4.615AlaAsp: 4.615 ± 0.674
6.923AlaGlu: 6.923 ± 0.655
3.127AlaPhe: 3.127 ± 0.418
8.859AlaGly: 8.859 ± 1.392
1.042AlaHis: 1.042 ± 0.256
5.285AlaIle: 5.285 ± 0.899
7.072AlaLys: 7.072 ± 1.031
7.817AlaLeu: 7.817 ± 1.41
3.722AlaMet: 3.722 ± 0.489
3.946AlaAsn: 3.946 ± 0.52
2.308AlaPro: 2.308 ± 0.428
3.052AlaGln: 3.052 ± 0.387
4.318AlaArg: 4.318 ± 0.6
4.69AlaSer: 4.69 ± 0.576
5.285AlaThr: 5.285 ± 0.633
6.03AlaVal: 6.03 ± 0.79
1.191AlaTrp: 1.191 ± 0.312
3.052AlaTyr: 3.052 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.149CysAla: 0.149 ± 0.15
0.074CysCys: 0.074 ± 0.068
0.447CysAsp: 0.447 ± 0.21
0.372CysGlu: 0.372 ± 0.248
0.149CysPhe: 0.149 ± 0.116
0.298CysGly: 0.298 ± 0.184
0.149CysHis: 0.149 ± 0.104
0.149CysIle: 0.149 ± 0.113
0.149CysLys: 0.149 ± 0.101
0.596CysLeu: 0.596 ± 0.241
0.0CysMet: 0.0 ± 0.0
0.298CysAsn: 0.298 ± 0.213
0.074CysPro: 0.074 ± 0.074
0.149CysGln: 0.149 ± 0.111
0.298CysArg: 0.298 ± 0.159
0.298CysSer: 0.298 ± 0.146
0.372CysThr: 0.372 ± 0.178
0.223CysVal: 0.223 ± 0.136
0.074CysTrp: 0.074 ± 0.068
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.477AspAla: 6.477 ± 0.674
0.223AspCys: 0.223 ± 0.112
4.243AspAsp: 4.243 ± 0.757
5.434AspGlu: 5.434 ± 0.81
2.829AspPhe: 2.829 ± 0.539
5.285AspGly: 5.285 ± 0.606
0.819AspHis: 0.819 ± 0.224
3.201AspIle: 3.201 ± 0.613
3.35AspLys: 3.35 ± 0.549
4.988AspLeu: 4.988 ± 0.632
1.787AspMet: 1.787 ± 0.359
2.829AspAsn: 2.829 ± 0.61
3.052AspPro: 3.052 ± 0.669
1.638AspGln: 1.638 ± 0.324
2.829AspArg: 2.829 ± 0.495
3.052AspSer: 3.052 ± 0.437
3.648AspThr: 3.648 ± 0.561
4.243AspVal: 4.243 ± 0.693
0.744AspTrp: 0.744 ± 0.2
1.489AspTyr: 1.489 ± 0.317
0.0AspXaa: 0.0 ± 0.0
Glu
5.434GluAla: 5.434 ± 0.81
0.149GluCys: 0.149 ± 0.097
3.722GluAsp: 3.722 ± 0.526
5.807GluGlu: 5.807 ± 1.037
2.978GluPhe: 2.978 ± 0.425
5.434GluGly: 5.434 ± 0.618
1.266GluHis: 1.266 ± 0.341
3.722GluIle: 3.722 ± 0.426
4.169GluLys: 4.169 ± 0.629
5.807GluLeu: 5.807 ± 0.742
2.308GluMet: 2.308 ± 0.455
2.382GluAsn: 2.382 ± 0.544
2.382GluPro: 2.382 ± 0.499
2.308GluGln: 2.308 ± 0.392
3.648GluArg: 3.648 ± 0.704
3.127GluSer: 3.127 ± 0.479
3.871GluThr: 3.871 ± 0.461
6.253GluVal: 6.253 ± 0.71
1.34GluTrp: 1.34 ± 0.373
2.01GluTyr: 2.01 ± 0.353
0.0GluXaa: 0.0 ± 0.0
Phe
2.903PheAla: 2.903 ± 0.586
0.372PheCys: 0.372 ± 0.196
3.127PheAsp: 3.127 ± 0.46
2.903PheGlu: 2.903 ± 0.702
1.191PhePhe: 1.191 ± 0.229
2.829PheGly: 2.829 ± 0.445
0.298PheHis: 0.298 ± 0.158
1.563PheIle: 1.563 ± 0.31
2.68PheLys: 2.68 ± 0.665
2.606PheLeu: 2.606 ± 0.429
0.819PheMet: 0.819 ± 0.209
1.563PheAsn: 1.563 ± 0.302
1.638PhePro: 1.638 ± 0.295
1.117PheGln: 1.117 ± 0.256
1.861PheArg: 1.861 ± 0.364
2.68PheSer: 2.68 ± 0.472
2.531PheThr: 2.531 ± 0.585
2.68PheVal: 2.68 ± 0.456
0.447PheTrp: 0.447 ± 0.196
1.489PheTyr: 1.489 ± 0.396
0.0PheXaa: 0.0 ± 0.0
Gly
6.849GlyAla: 6.849 ± 0.806
0.372GlyCys: 0.372 ± 0.217
4.392GlyAsp: 4.392 ± 0.616
4.094GlyGlu: 4.094 ± 0.472
3.648GlyPhe: 3.648 ± 0.575
5.434GlyGly: 5.434 ± 0.748
0.893GlyHis: 0.893 ± 0.328
4.913GlyIle: 4.913 ± 0.962
3.722GlyLys: 3.722 ± 0.496
5.658GlyLeu: 5.658 ± 1.299
2.308GlyMet: 2.308 ± 0.456
2.233GlyAsn: 2.233 ± 0.475
2.159GlyPro: 2.159 ± 0.471
2.159GlyGln: 2.159 ± 0.326
2.978GlyArg: 2.978 ± 0.422
4.69GlySer: 4.69 ± 0.7
6.253GlyThr: 6.253 ± 0.766
6.923GlyVal: 6.923 ± 0.798
1.266GlyTrp: 1.266 ± 0.438
3.871GlyTyr: 3.871 ± 0.619
0.0GlyXaa: 0.0 ± 0.0
His
0.968HisAla: 0.968 ± 0.206
0.149HisCys: 0.149 ± 0.135
0.968HisAsp: 0.968 ± 0.344
0.521HisGlu: 0.521 ± 0.216
0.149HisPhe: 0.149 ± 0.093
1.489HisGly: 1.489 ± 0.483
0.447HisHis: 0.447 ± 0.182
1.266HisIle: 1.266 ± 0.312
0.298HisLys: 0.298 ± 0.184
1.266HisLeu: 1.266 ± 0.279
0.521HisMet: 0.521 ± 0.192
0.223HisAsn: 0.223 ± 0.132
0.819HisPro: 0.819 ± 0.246
0.893HisGln: 0.893 ± 0.231
0.447HisArg: 0.447 ± 0.18
0.819HisSer: 0.819 ± 0.234
0.893HisThr: 0.893 ± 0.369
0.968HisVal: 0.968 ± 0.31
0.298HisTrp: 0.298 ± 0.138
1.266HisTyr: 1.266 ± 0.34
0.0HisXaa: 0.0 ± 0.0
Ile
4.69IleAla: 4.69 ± 0.751
0.149IleCys: 0.149 ± 0.13
4.02IleAsp: 4.02 ± 0.367
4.541IleGlu: 4.541 ± 0.579
1.712IlePhe: 1.712 ± 0.411
3.946IleGly: 3.946 ± 0.759
0.893IleHis: 0.893 ± 0.321
2.754IleIle: 2.754 ± 0.591
3.573IleLys: 3.573 ± 0.768
4.094IleLeu: 4.094 ± 0.681
1.042IleMet: 1.042 ± 0.226
1.787IleAsn: 1.787 ± 0.319
1.787IlePro: 1.787 ± 0.272
1.638IleGln: 1.638 ± 0.307
2.531IleArg: 2.531 ± 0.474
3.573IleSer: 3.573 ± 0.482
3.127IleThr: 3.127 ± 0.465
3.648IleVal: 3.648 ± 0.49
0.893IleTrp: 0.893 ± 0.285
1.712IleTyr: 1.712 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
6.477LysAla: 6.477 ± 0.741
0.223LysCys: 0.223 ± 0.121
2.978LysAsp: 2.978 ± 0.549
3.499LysGlu: 3.499 ± 0.426
2.233LysPhe: 2.233 ± 0.536
3.573LysGly: 3.573 ± 0.782
1.266LysHis: 1.266 ± 0.363
3.276LysIle: 3.276 ± 0.549
5.583LysLys: 5.583 ± 0.851
6.774LysLeu: 6.774 ± 0.998
1.861LysMet: 1.861 ± 0.581
2.084LysAsn: 2.084 ± 0.473
2.754LysPro: 2.754 ± 0.642
2.754LysGln: 2.754 ± 0.489
3.573LysArg: 3.573 ± 0.537
4.02LysSer: 4.02 ± 0.545
3.797LysThr: 3.797 ± 0.465
4.243LysVal: 4.243 ± 0.485
1.042LysTrp: 1.042 ± 0.33
2.01LysTyr: 2.01 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
8.263LeuAla: 8.263 ± 1.692
0.298LeuCys: 0.298 ± 0.188
5.36LeuAsp: 5.36 ± 0.622
5.211LeuGlu: 5.211 ± 0.673
2.457LeuPhe: 2.457 ± 0.532
5.955LeuGly: 5.955 ± 0.871
0.968LeuHis: 0.968 ± 0.285
3.797LeuIle: 3.797 ± 0.622
5.658LeuLys: 5.658 ± 0.674
6.104LeuLeu: 6.104 ± 0.603
2.159LeuMet: 2.159 ± 0.478
4.467LeuAsn: 4.467 ± 0.659
3.127LeuPro: 3.127 ± 0.469
2.382LeuGln: 2.382 ± 0.451
3.797LeuArg: 3.797 ± 0.697
5.509LeuSer: 5.509 ± 1.081
6.849LeuThr: 6.849 ± 0.79
5.807LeuVal: 5.807 ± 0.675
0.893LeuTrp: 0.893 ± 0.258
2.68LeuTyr: 2.68 ± 0.539
0.0LeuXaa: 0.0 ± 0.0
Met
2.531MetAla: 2.531 ± 0.387
0.074MetCys: 0.074 ± 0.076
2.233MetAsp: 2.233 ± 0.328
2.01MetGlu: 2.01 ± 0.536
0.819MetPhe: 0.819 ± 0.259
1.638MetGly: 1.638 ± 0.314
0.223MetHis: 0.223 ± 0.134
1.117MetIle: 1.117 ± 0.299
1.34MetLys: 1.34 ± 0.326
2.382MetLeu: 2.382 ± 0.531
0.893MetMet: 0.893 ± 0.302
1.266MetAsn: 1.266 ± 0.294
1.117MetPro: 1.117 ± 0.322
0.67MetGln: 0.67 ± 0.231
1.563MetArg: 1.563 ± 0.34
1.712MetSer: 1.712 ± 0.336
2.084MetThr: 2.084 ± 0.433
1.787MetVal: 1.787 ± 0.412
0.149MetTrp: 0.149 ± 0.103
0.819MetTyr: 0.819 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
4.169AsnAla: 4.169 ± 0.711
0.149AsnCys: 0.149 ± 0.098
2.754AsnAsp: 2.754 ± 0.429
3.424AsnGlu: 3.424 ± 0.654
1.489AsnPhe: 1.489 ± 0.415
3.573AsnGly: 3.573 ± 0.647
0.67AsnHis: 0.67 ± 0.189
2.382AsnIle: 2.382 ± 0.374
2.308AsnLys: 2.308 ± 0.322
3.573AsnLeu: 3.573 ± 0.636
0.447AsnMet: 0.447 ± 0.185
1.936AsnAsn: 1.936 ± 0.551
2.382AsnPro: 2.382 ± 0.428
1.489AsnGln: 1.489 ± 0.28
2.829AsnArg: 2.829 ± 0.6
2.68AsnSer: 2.68 ± 0.494
2.606AsnThr: 2.606 ± 0.376
2.68AsnVal: 2.68 ± 0.482
0.149AsnTrp: 0.149 ± 0.094
1.787AsnTyr: 1.787 ± 0.359
0.0AsnXaa: 0.0 ± 0.0
Pro
3.499ProAla: 3.499 ± 0.602
0.298ProCys: 0.298 ± 0.169
2.754ProAsp: 2.754 ± 0.57
2.68ProGlu: 2.68 ± 0.502
1.787ProPhe: 1.787 ± 0.286
2.829ProGly: 2.829 ± 0.565
0.521ProHis: 0.521 ± 0.205
1.414ProIle: 1.414 ± 0.313
2.308ProLys: 2.308 ± 0.363
2.308ProLeu: 2.308 ± 0.382
1.191ProMet: 1.191 ± 0.308
2.01ProAsn: 2.01 ± 0.493
1.563ProPro: 1.563 ± 0.308
1.117ProGln: 1.117 ± 0.335
1.712ProArg: 1.712 ± 0.526
3.276ProSer: 3.276 ± 0.714
3.648ProThr: 3.648 ± 0.593
3.127ProVal: 3.127 ± 0.521
0.521ProTrp: 0.521 ± 0.23
1.34ProTyr: 1.34 ± 0.328
0.0ProXaa: 0.0 ± 0.0
Gln
3.573GlnAla: 3.573 ± 0.563
0.149GlnCys: 0.149 ± 0.114
1.638GlnAsp: 1.638 ± 0.39
1.414GlnGlu: 1.414 ± 0.373
1.489GlnPhe: 1.489 ± 0.302
2.01GlnGly: 2.01 ± 0.428
0.819GlnHis: 0.819 ± 0.307
2.382GlnIle: 2.382 ± 0.428
1.638GlnLys: 1.638 ± 0.272
3.127GlnLeu: 3.127 ± 0.716
0.744GlnMet: 0.744 ± 0.277
1.414GlnAsn: 1.414 ± 0.309
1.563GlnPro: 1.563 ± 0.336
1.266GlnGln: 1.266 ± 0.403
2.159GlnArg: 2.159 ± 0.425
1.489GlnSer: 1.489 ± 0.345
2.084GlnThr: 2.084 ± 0.474
2.308GlnVal: 2.308 ± 0.364
0.298GlnTrp: 0.298 ± 0.177
1.34GlnTyr: 1.34 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
4.392ArgAla: 4.392 ± 0.585
0.372ArgCys: 0.372 ± 0.156
2.457ArgAsp: 2.457 ± 0.409
3.276ArgGlu: 3.276 ± 0.373
1.34ArgPhe: 1.34 ± 0.242
2.68ArgGly: 2.68 ± 0.41
0.744ArgHis: 0.744 ± 0.247
2.606ArgIle: 2.606 ± 0.68
4.094ArgLys: 4.094 ± 0.497
4.988ArgLeu: 4.988 ± 0.86
1.489ArgMet: 1.489 ± 0.4
2.68ArgAsn: 2.68 ± 0.382
2.233ArgPro: 2.233 ± 0.565
1.787ArgGln: 1.787 ± 0.466
3.276ArgArg: 3.276 ± 0.72
2.68ArgSer: 2.68 ± 0.437
3.35ArgThr: 3.35 ± 0.733
3.648ArgVal: 3.648 ± 0.683
0.521ArgTrp: 0.521 ± 0.21
1.042ArgTyr: 1.042 ± 0.252
0.0ArgXaa: 0.0 ± 0.0
Ser
5.732SerAla: 5.732 ± 0.925
0.372SerCys: 0.372 ± 0.258
4.02SerAsp: 4.02 ± 0.506
3.722SerGlu: 3.722 ± 0.596
2.233SerPhe: 2.233 ± 0.337
4.243SerGly: 4.243 ± 0.716
0.819SerHis: 0.819 ± 0.294
2.978SerIle: 2.978 ± 0.445
3.946SerLys: 3.946 ± 0.613
4.541SerLeu: 4.541 ± 0.552
1.042SerMet: 1.042 ± 0.309
2.903SerAsn: 2.903 ± 0.422
2.308SerPro: 2.308 ± 0.341
2.308SerGln: 2.308 ± 0.444
2.531SerArg: 2.531 ± 0.51
4.318SerSer: 4.318 ± 0.637
4.615SerThr: 4.615 ± 0.892
4.243SerVal: 4.243 ± 0.57
1.266SerTrp: 1.266 ± 0.308
2.531SerTyr: 2.531 ± 0.613
0.0SerXaa: 0.0 ± 0.0
Thr
6.253ThrAla: 6.253 ± 0.808
0.0ThrCys: 0.0 ± 0.0
4.541ThrAsp: 4.541 ± 0.658
4.318ThrGlu: 4.318 ± 0.663
2.68ThrPhe: 2.68 ± 0.515
6.104ThrGly: 6.104 ± 0.851
0.968ThrHis: 0.968 ± 0.295
2.68ThrIle: 2.68 ± 0.531
3.424ThrLys: 3.424 ± 0.391
5.658ThrLeu: 5.658 ± 0.652
1.34ThrMet: 1.34 ± 0.308
2.382ThrAsn: 2.382 ± 0.425
4.243ThrPro: 4.243 ± 0.781
2.382ThrGln: 2.382 ± 0.442
3.424ThrArg: 3.424 ± 0.65
4.467ThrSer: 4.467 ± 0.669
5.658ThrThr: 5.658 ± 1.133
6.625ThrVal: 6.625 ± 1.089
0.893ThrTrp: 0.893 ± 0.25
2.457ThrTyr: 2.457 ± 0.566
0.0ThrXaa: 0.0 ± 0.0
Val
6.402ValAla: 6.402 ± 0.482
0.223ValCys: 0.223 ± 0.13
4.318ValAsp: 4.318 ± 0.559
5.062ValGlu: 5.062 ± 0.616
2.68ValPhe: 2.68 ± 0.551
5.36ValGly: 5.36 ± 0.714
1.117ValHis: 1.117 ± 0.194
4.094ValIle: 4.094 ± 0.568
5.062ValLys: 5.062 ± 0.627
6.477ValLeu: 6.477 ± 0.864
1.638ValMet: 1.638 ± 0.278
3.722ValAsn: 3.722 ± 0.371
2.606ValPro: 2.606 ± 0.588
1.638ValGln: 1.638 ± 0.395
3.722ValArg: 3.722 ± 0.515
4.243ValSer: 4.243 ± 0.631
6.551ValThr: 6.551 ± 0.938
5.36ValVal: 5.36 ± 0.537
1.414ValTrp: 1.414 ± 0.431
3.35ValTyr: 3.35 ± 0.542
0.0ValXaa: 0.0 ± 0.0
Trp
0.521TrpAla: 0.521 ± 0.178
0.074TrpCys: 0.074 ± 0.077
1.414TrpAsp: 1.414 ± 0.469
0.744TrpGlu: 0.744 ± 0.203
0.447TrpPhe: 0.447 ± 0.206
1.34TrpGly: 1.34 ± 0.406
0.372TrpHis: 0.372 ± 0.159
0.298TrpIle: 0.298 ± 0.137
0.893TrpLys: 0.893 ± 0.304
1.117TrpLeu: 1.117 ± 0.312
0.298TrpMet: 0.298 ± 0.152
1.191TrpAsn: 1.191 ± 0.392
0.223TrpPro: 0.223 ± 0.125
0.447TrpGln: 0.447 ± 0.171
0.521TrpArg: 0.521 ± 0.271
0.968TrpSer: 0.968 ± 0.461
1.266TrpThr: 1.266 ± 0.41
1.117TrpVal: 1.117 ± 0.349
0.149TrpTrp: 0.149 ± 0.125
0.372TrpTyr: 0.372 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.424TyrAla: 3.424 ± 0.663
0.223TyrCys: 0.223 ± 0.152
2.159TyrAsp: 2.159 ± 0.415
2.159TyrGlu: 2.159 ± 0.361
1.861TyrPhe: 1.861 ± 0.451
1.936TyrGly: 1.936 ± 0.483
0.372TyrHis: 0.372 ± 0.145
2.233TyrIle: 2.233 ± 0.433
2.754TyrLys: 2.754 ± 0.536
1.861TyrLeu: 1.861 ± 0.386
0.744TyrMet: 0.744 ± 0.233
2.159TyrAsn: 2.159 ± 0.409
1.563TyrPro: 1.563 ± 0.376
1.712TyrGln: 1.712 ± 0.266
1.712TyrArg: 1.712 ± 0.348
2.382TyrSer: 2.382 ± 0.436
2.01TyrThr: 2.01 ± 0.381
3.127TyrVal: 3.127 ± 0.387
0.223TyrTrp: 0.223 ± 0.148
1.414TyrTyr: 1.414 ± 0.394
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13434 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski