Drancourtella sp. An57
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3069 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y3WA39|A0A1Y3WA39_9FIRM 50S ribosomal protein L2 OS=Drancourtella sp. An57 OX=1965647 GN=rplB PE=3 SV=1
MM1 pKa = 7.18 KK2 pKa = 10.33 RR3 pKa = 11.84 KK4 pKa = 9.33 LAVVCIMMLSATTILSGCGGSSDD27 pKa = 4.61 DD28 pKa = 4.12 SSSGGKK34 pKa = 7.9 TKK36 pKa = 10.25 IRR38 pKa = 11.84 FATWDD43 pKa = 3.34 VAEE46 pKa = 5.53 DD47 pKa = 3.33 VDD49 pKa = 4.44 KK50 pKa = 11.31 QQEE53 pKa = 4.19 LVDD56 pKa = 4.19 KK57 pKa = 10.82 FNEE60 pKa = 3.91 EE61 pKa = 3.76 HH62 pKa = 7.68 DD63 pKa = 4.91 DD64 pKa = 3.99 IEE66 pKa = 4.66 VTLEE70 pKa = 4.04 AYY72 pKa = 10.28 GSDD75 pKa = 3.71 FDD77 pKa = 4.38 TKK79 pKa = 10.34 ISAGMGSGDD88 pKa = 4.1 TPDD91 pKa = 4.25 VMYY94 pKa = 9.35 MWNYY98 pKa = 7.71 PAYY101 pKa = 10.07 YY102 pKa = 10.18 DD103 pKa = 4.15 GLEE106 pKa = 4.33 PLDD109 pKa = 5.09 DD110 pKa = 4.73 YY111 pKa = 11.25 IAEE114 pKa = 4.57 EE115 pKa = 4.14 GDD117 pKa = 3.64 EE118 pKa = 4.32 YY119 pKa = 11.44 KK120 pKa = 11.26 SNFYY124 pKa = 8.72 NTLWDD129 pKa = 3.96 YY130 pKa = 12.15 NMMDD134 pKa = 3.36 GSTYY138 pKa = 10.28 GIPVGFTTHH147 pKa = 6.58 CLFYY151 pKa = 11.12 NKK153 pKa = 10.23 DD154 pKa = 3.3 IFDD157 pKa = 4.11 EE158 pKa = 4.85 AGVEE162 pKa = 4.18 YY163 pKa = 8.47 PTADD167 pKa = 3.53 WTWDD171 pKa = 3.66 DD172 pKa = 3.95 LQAAAKK178 pKa = 8.7 TISEE182 pKa = 4.22 KK183 pKa = 10.28 TDD185 pKa = 2.87 AKK187 pKa = 10.95 GFSFQMKK194 pKa = 8.93 PDD196 pKa = 4.18 PYY198 pKa = 10.78 DD199 pKa = 3.45 FEE201 pKa = 4.59 MYY203 pKa = 10.03 LWSNGTSYY211 pKa = 11.19 CDD213 pKa = 3.59 EE214 pKa = 4.41 NGEE217 pKa = 3.78 MDD219 pKa = 4.36 GYY221 pKa = 10.6 INSEE225 pKa = 3.79 EE226 pKa = 4.17 SQEE229 pKa = 4.0 VFQMFQDD236 pKa = 3.93 MEE238 pKa = 4.4 EE239 pKa = 4.1 EE240 pKa = 5.31 GYY242 pKa = 8.77 ATATEE247 pKa = 4.33 GDD249 pKa = 3.66 GTDD252 pKa = 3.34 EE253 pKa = 4.09 FRR255 pKa = 11.84 AGSTAMYY262 pKa = 10.37 VYY264 pKa = 10.57 GSWSINTLNEE274 pKa = 4.14 DD275 pKa = 3.49 GVNYY279 pKa = 10.41 GVTTIPAFADD289 pKa = 3.34 AGQDD293 pKa = 3.47 SVSILSSSGLSMSKK307 pKa = 10.57 DD308 pKa = 3.69 SEE310 pKa = 4.45 HH311 pKa = 7.4 KK312 pKa = 9.93 DD313 pKa = 3.39 AAWEE317 pKa = 4.51 FIKK320 pKa = 11.0 YY321 pKa = 6.25 WTNEE325 pKa = 3.74 EE326 pKa = 4.18 CNKK329 pKa = 10.15 ARR331 pKa = 11.84 IGTEE335 pKa = 3.9 LPVLNTVVEE344 pKa = 4.5 SEE346 pKa = 4.59 GIMEE350 pKa = 4.18 QEE352 pKa = 4.43 EE353 pKa = 4.43 YY354 pKa = 11.38 APFYY358 pKa = 11.15 TMLEE362 pKa = 4.15 QSSGHH367 pKa = 5.65 TPASFLMEE375 pKa = 4.12 SWSEE379 pKa = 3.9 VSEE382 pKa = 4.01 NLSLSFEE389 pKa = 4.47 QIFNPSSMQDD399 pKa = 3.02 VSEE402 pKa = 4.4 VLDD405 pKa = 3.87 SAVKK409 pKa = 10.31
Molecular weight: 45.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.808
IPC_protein 3.821
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.935
Patrickios 0.731
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A1Y3W3X1|A0A1Y3W3X1_9FIRM Alanine--tRNA ligase OS=Drancourtella sp. An57 OX=1965647 GN=B5G11_14770 PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.86 KK9 pKa = 8.2 RR10 pKa = 11.84 SRR12 pKa = 11.84 AKK14 pKa = 9.27 VHH16 pKa = 5.81 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTAGGRR28 pKa = 11.84 KK29 pKa = 8.71 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.92 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3069
0
3069
969582
37
1974
315.9
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.263 ± 0.047
1.47 ± 0.017
5.503 ± 0.034
8.458 ± 0.061
4.064 ± 0.031
7.221 ± 0.043
1.811 ± 0.02
7.142 ± 0.046
6.765 ± 0.04
8.928 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.141 ± 0.023
3.842 ± 0.031
3.371 ± 0.026
3.376 ± 0.023
4.934 ± 0.038
5.538 ± 0.039
5.316 ± 0.034
6.77 ± 0.037
0.966 ± 0.015
4.119 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here