Rotavirus A EC2184/ECU/G11P[6]

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A; Rotavirus G11

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0TZ89|D0TZ89_9REOV NSP1 (Fragment) OS=Rotavirus A EC2184/ECU/G11P[6] OX=672377 GN=NSP1 PE=3 SV=1
MM1 pKa = 7.26YY2 pKa = 10.2GIEE5 pKa = 4.22YY6 pKa = 7.59TTILTFLISLVFVNYY21 pKa = 9.56ILKK24 pKa = 10.44SVTRR28 pKa = 11.84TMDD31 pKa = 3.79FIIYY35 pKa = 9.48RR36 pKa = 11.84FLFIIVVLAPLIKK49 pKa = 8.78TQNYY53 pKa = 9.29GINLPITGSMDD64 pKa = 3.38TPYY67 pKa = 10.67TNSTMSEE74 pKa = 4.1TFLTSTLCLYY84 pKa = 10.87YY85 pKa = 9.96PGEE88 pKa = 4.41AATQIADD95 pKa = 4.71DD96 pKa = 3.88EE97 pKa = 4.7WKK99 pKa = 9.02DD100 pKa = 3.55TLSQLFLTKK109 pKa = 10.05GWPTGSVYY117 pKa = 10.44FKK119 pKa = 10.0EE120 pKa = 4.33YY121 pKa = 10.32TDD123 pKa = 3.31IASFSVDD130 pKa = 3.05PQLYY134 pKa = 8.88CDD136 pKa = 3.82YY137 pKa = 10.47NIVIMKK143 pKa = 10.23YY144 pKa = 10.34DD145 pKa = 3.84GNSQLDD151 pKa = 3.68MSEE154 pKa = 4.08LADD157 pKa = 5.13LILNEE162 pKa = 4.24WLCNPMDD169 pKa = 3.6IALYY173 pKa = 9.95YY174 pKa = 8.76YY175 pKa = 9.53QQIDD179 pKa = 4.06EE180 pKa = 4.28ANKK183 pKa = 7.88WISMGTSCTIKK194 pKa = 10.29VCPLNTQTLGVGCLTTDD211 pKa = 3.05PTTFEE216 pKa = 4.24EE217 pKa = 4.57VASAEE222 pKa = 4.1KK223 pKa = 10.57LAITDD228 pKa = 3.39VVDD231 pKa = 4.37GVNHH235 pKa = 6.65KK236 pKa = 10.85LDD238 pKa = 3.69VTTATCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 4.01NVAVIQVGGSNILDD270 pKa = 3.33ITADD274 pKa = 3.68PTTAPQTEE282 pKa = 3.66RR283 pKa = 11.84MMRR286 pKa = 11.84INWKK290 pKa = 9.54KK291 pKa = 7.26WWQVFYY297 pKa = 10.66TIVDD301 pKa = 3.57YY302 pKa = 11.48VNQIVQVMSKK312 pKa = 8.7QSRR315 pKa = 11.84SS316 pKa = 3.29

Molecular weight: 35.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0TZ93|D0TZ93_9REOV Non-structural protein 2 OS=Rotavirus A EC2184/ECU/G11P[6] OX=672377 GN=NSP2 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 11.97KK18 pKa = 10.64FIPFNSLAIKK28 pKa = 10.62CMLTAKK34 pKa = 9.91VDD36 pKa = 3.91KK37 pKa = 10.63KK38 pKa = 11.12DD39 pKa = 3.04QDD41 pKa = 3.13KK42 pKa = 10.47FYY44 pKa = 11.27NSIVYY49 pKa = 9.73GIAPPQQFKK58 pKa = 10.77KK59 pKa = 10.5RR60 pKa = 11.84YY61 pKa = 5.68NTNDD65 pKa = 2.8NSRR68 pKa = 11.84GMNYY72 pKa = 6.85EE73 pKa = 3.33TSMFNKK79 pKa = 9.24VAMLICEE86 pKa = 4.51ALNSIKK92 pKa = 9.45VTQSDD97 pKa = 4.06VANVLSRR104 pKa = 11.84VVSVRR109 pKa = 11.84HH110 pKa = 5.93LEE112 pKa = 3.89NLVLRR117 pKa = 11.84KK118 pKa = 9.59EE119 pKa = 4.02NHH121 pKa = 5.63QDD123 pKa = 3.26VLFHH127 pKa = 7.13SKK129 pKa = 10.37EE130 pKa = 4.08LLLKK134 pKa = 10.57AVLIAIGQSKK144 pKa = 10.17EE145 pKa = 3.74IEE147 pKa = 4.2TTATAEE153 pKa = 3.87GGEE156 pKa = 4.08IVFQNAAFTMWKK168 pKa = 8.49LTYY171 pKa = 9.82LDD173 pKa = 4.31HH174 pKa = 8.04KK175 pKa = 10.88LMPILDD181 pKa = 3.78QNFIEE186 pKa = 5.0YY187 pKa = 10.32KK188 pKa = 8.98ITLNEE193 pKa = 4.58DD194 pKa = 3.19KK195 pKa = 10.16PISDD199 pKa = 3.76MCVKK203 pKa = 10.38EE204 pKa = 4.35LVAEE208 pKa = 4.68LRR210 pKa = 11.84WQYY213 pKa = 11.68NRR215 pKa = 11.84FAVITHH221 pKa = 6.3GKK223 pKa = 7.43GHH225 pKa = 5.73YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.18ADD239 pKa = 3.4RR240 pKa = 11.84VFATYY245 pKa = 10.71KK246 pKa = 10.95NNVKK250 pKa = 10.31SGNITDD256 pKa = 5.52FNLLDD261 pKa = 5.27QRR263 pKa = 11.84IIWQNWYY270 pKa = 10.72AFTSSMKK277 pKa = 10.28QGNTLDD283 pKa = 3.59VCKK286 pKa = 10.66KK287 pKa = 10.71LLFQKK292 pKa = 9.76MKK294 pKa = 10.47QEE296 pKa = 4.09KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.05MDD310 pKa = 3.64EE311 pKa = 4.1VSHH314 pKa = 6.83VGII317 pKa = 4.83

Molecular weight: 36.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3636

175

601

330.5

38.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.923 ± 0.545

1.568 ± 0.446

6.628 ± 0.444

5.776 ± 0.539

4.07 ± 0.398

3.273 ± 0.352

1.815 ± 0.309

7.206 ± 0.312

7.206 ± 0.751

8.801 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.163 ± 0.293

7.096 ± 0.479

3.328 ± 0.375

4.318 ± 0.415

4.373 ± 0.339

7.398 ± 0.867

6.573 ± 0.714

6.436 ± 0.265

1.348 ± 0.174

4.703 ± 0.468
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski