Halorubrum pleomorphic virus 9
Average proteome isoelectric point is 5.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S7HQK6|A0A3S7HQK6_9VIRU Uncharacterized protein OS=Halorubrum pleomorphic virus 9 OX=2126525 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 5.04 AMSSIWDD9 pKa = 3.8 GEE11 pKa = 4.1 DD12 pKa = 3.28 TIAGVIWILTSIGSINWGLLEE33 pKa = 4.2 FFDD36 pKa = 4.37 YY37 pKa = 11.26 NAVSEE42 pKa = 4.73 LGTALGSPTVATVVYY57 pKa = 9.84 GAVAVAGVVTLADD70 pKa = 3.07 HH71 pKa = 6.98 AGFYY75 pKa = 10.42 DD76 pKa = 3.75 VPDD79 pKa = 3.38 VVDD82 pKa = 4.07 QIRR85 pKa = 11.84 GDD87 pKa = 3.59 GGDD90 pKa = 3.15
Molecular weight: 9.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.013
IPC2_protein 3.35
IPC_protein 3.287
Toseland 3.096
ProMoST 3.452
Dawson 3.338
Bjellqvist 3.579
Wikipedia 3.35
Rodwell 3.147
Grimsley 3.02
Solomon 3.261
Lehninger 3.223
Nozaki 3.49
DTASelect 3.706
Thurlkill 3.198
EMBOSS 3.35
Sillero 3.427
Patrickios 0.477
IPC_peptide 3.249
IPC2_peptide 3.389
IPC2.peptide.svr19 3.726
Protein with the highest isoelectric point:
>tr|A0A3S7IAM3|A0A3S7IAM3_9VIRU Uncharacterized protein OS=Halorubrum pleomorphic virus 9 OX=2126525 PE=4 SV=1
MM1 pKa = 7.15 RR2 pKa = 11.84 TASVRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 AASGAKK15 pKa = 9.12 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 GRR20 pKa = 11.84 VTADD24 pKa = 3.31 PVPGRR29 pKa = 11.84 TAGSRR34 pKa = 11.84 EE35 pKa = 3.85 RR36 pKa = 11.84 TGSSRR41 pKa = 11.84 QPSVPRR47 pKa = 11.84 LARR50 pKa = 11.84 VAGRR54 pKa = 11.84 TGLRR58 pKa = 11.84 VRR60 pKa = 11.84 RR61 pKa = 11.84 VDD63 pKa = 3.48 VFSGRR68 pKa = 11.84 ASLADD73 pKa = 3.85 PDD75 pKa = 4.02 PRR77 pKa = 11.84 VALGTAVRR85 pKa = 11.84 AVVLVAVDD93 pKa = 3.42 ATKK96 pKa = 10.17 VRR98 pKa = 11.84 GYY100 pKa = 11.1 VGVATKK106 pKa = 10.41 AVAPATVRR114 pKa = 11.84 SEE116 pKa = 3.78 QHH118 pKa = 6.28 HH119 pKa = 7.25 ADD121 pKa = 3.6 TTSANADD128 pKa = 3.56 RR129 pKa = 11.84 GTVVTTGLILARR141 pKa = 11.84 TKK143 pKa = 10.32 HH144 pKa = 6.05 ANGASRR150 pKa = 11.84 EE151 pKa = 4.17 LEE153 pKa = 3.76 RR154 pKa = 11.84 RR155 pKa = 11.84 NWVAGEE161 pKa = 4.08 SQPTNSFGRR170 pKa = 11.84 SPNSIKK176 pKa = 10.67 VGQHH180 pKa = 4.28 GG181 pKa = 3.45
Molecular weight: 19.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 10.335
IPC_protein 11.871
Toseland 12.032
ProMoST 12.53
Dawson 12.032
Bjellqvist 12.032
Wikipedia 12.515
Rodwell 11.652
Grimsley 12.076
Solomon 12.53
Lehninger 12.427
Nozaki 12.032
DTASelect 12.032
Thurlkill 12.032
EMBOSS 12.53
Sillero 12.032
Patrickios 11.374
IPC_peptide 12.53
IPC2_peptide 11.52
IPC2.peptide.svr19 9.417
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
4916
45
584
175.6
19.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.337 ± 0.655
0.488 ± 0.157
6.937 ± 0.555
7.771 ± 0.703
2.766 ± 0.436
8.381 ± 0.546
1.607 ± 0.281
4.963 ± 0.643
2.665 ± 0.342
8.401 ± 0.504
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.648 ± 0.167
3.072 ± 0.384
4.394 ± 0.563
2.909 ± 0.325
7.343 ± 0.991
7.323 ± 0.419
6.509 ± 0.667
9.133 ± 0.534
1.404 ± 0.177
2.95 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here