Streptomyces sp. TM32

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1RVR8|A0A4Q1RVR8_9ACTN 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Streptomyces sp. TM32 OX=1652669 GN=fabH PE=3 SV=1
MM1 pKa = 7.76PEE3 pKa = 3.4VTAPYY8 pKa = 10.28QPGTPCWVDD17 pKa = 3.59LAAPDD22 pKa = 3.44QQAAIDD28 pKa = 4.64FYY30 pKa = 11.42SQVFGWAGEE39 pKa = 3.95IGPAEE44 pKa = 3.99TGGYY48 pKa = 9.06AVCRR52 pKa = 11.84LNDD55 pKa = 3.64KK56 pKa = 10.22PVAGIMAAVPMGGQPAPPTVWTTYY80 pKa = 10.41LAATDD85 pKa = 4.12ADD87 pKa = 3.59ATAQAVTKK95 pKa = 10.8AGGTVLMPVMDD106 pKa = 3.92VMTLGRR112 pKa = 11.84MAIVADD118 pKa = 3.71PTGAVCGLWQPVDD131 pKa = 3.97FPGAGIVNEE140 pKa = 4.19PGALIWNEE148 pKa = 4.19LNTTDD153 pKa = 4.47PSAAGAFYY161 pKa = 10.42QVALGIEE168 pKa = 4.16NAPMEE173 pKa = 4.66GAEE176 pKa = 4.43HH177 pKa = 6.73YY178 pKa = 9.76YY179 pKa = 11.27ALTVDD184 pKa = 4.01GRR186 pKa = 11.84PVGGMQPMSEE196 pKa = 4.26QMPADD201 pKa = 4.28TPSHH205 pKa = 6.08WLPYY209 pKa = 10.21FSVTDD214 pKa = 3.33TDD216 pKa = 3.7ATVKK220 pKa = 10.48KK221 pKa = 8.99VTAAGGAALQQPFDD235 pKa = 4.11MIAGRR240 pKa = 11.84MAVVTDD246 pKa = 3.74PQGATFAVINPKK258 pKa = 10.14PMNQGG263 pKa = 2.86

Molecular weight: 27.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1NU03|A0A4V1NU03_9ACTN Aldolase OS=Streptomyces sp. TM32 OX=1652669 GN=EST92_01160 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.36GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5851

0

5851

1906603

27

6692

325.9

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.901 ± 0.045

0.842 ± 0.01

5.832 ± 0.028

5.545 ± 0.034

2.692 ± 0.016

9.544 ± 0.035

2.41 ± 0.017

3.157 ± 0.022

2.243 ± 0.029

10.413 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.754 ± 0.014

1.713 ± 0.017

6.254 ± 0.033

2.919 ± 0.025

8.163 ± 0.038

4.947 ± 0.023

6.051 ± 0.028

8.132 ± 0.033

1.478 ± 0.014

2.01 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski