Amino acid dipepetide frequency for Streptomyces sp. TM32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.743AlaAla: 22.743 ± 0.157
1.1AlaCys: 1.1 ± 0.028
8.32AlaAsp: 8.32 ± 0.073
8.741AlaGlu: 8.741 ± 0.096
3.52AlaPhe: 3.52 ± 0.041
13.902AlaGly: 13.902 ± 0.122
3.12AlaHis: 3.12 ± 0.043
3.321AlaIle: 3.321 ± 0.05
3.03AlaLys: 3.03 ± 0.056
14.97AlaLeu: 14.97 ± 0.111
2.539AlaMet: 2.539 ± 0.039
1.862AlaAsn: 1.862 ± 0.033
7.83AlaPro: 7.83 ± 0.09
3.99AlaGln: 3.99 ± 0.057
10.803AlaArg: 10.803 ± 0.103
5.565AlaSer: 5.565 ± 0.059
7.112AlaThr: 7.112 ± 0.063
12.157AlaVal: 12.157 ± 0.095
1.733AlaTrp: 1.733 ± 0.034
2.649AlaTyr: 2.649 ± 0.038
0.001AlaXaa: 0.001 ± 0.001
Cys
1.234CysAla: 1.234 ± 0.029
0.106CysCys: 0.106 ± 0.009
0.504CysAsp: 0.504 ± 0.016
0.4CysGlu: 0.4 ± 0.015
0.234CysPhe: 0.234 ± 0.012
1.014CysGly: 1.014 ± 0.028
0.215CysHis: 0.215 ± 0.011
0.208CysIle: 0.208 ± 0.01
0.125CysLys: 0.125 ± 0.009
0.769CysLeu: 0.769 ± 0.02
0.132CysMet: 0.132 ± 0.008
0.144CysAsn: 0.144 ± 0.009
0.523CysPro: 0.523 ± 0.021
0.182CysGln: 0.182 ± 0.009
0.647CysArg: 0.647 ± 0.017
0.428CysSer: 0.428 ± 0.016
0.586CysThr: 0.586 ± 0.02
0.66CysVal: 0.66 ± 0.018
0.133CysTrp: 0.133 ± 0.008
0.174CysTyr: 0.174 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.721AspAla: 7.721 ± 0.074
0.416AspCys: 0.416 ± 0.017
3.424AspAsp: 3.424 ± 0.048
3.774AspGlu: 3.774 ± 0.054
1.581AspPhe: 1.581 ± 0.033
6.415AspGly: 6.415 ± 0.076
1.462AspHis: 1.462 ± 0.029
1.818AspIle: 1.818 ± 0.034
1.245AspLys: 1.245 ± 0.032
6.005AspLeu: 6.005 ± 0.061
0.776AspMet: 0.776 ± 0.017
0.919AspAsn: 0.919 ± 0.022
4.446AspPro: 4.446 ± 0.051
1.544AspGln: 1.544 ± 0.029
4.962AspArg: 4.962 ± 0.058
2.405AspSer: 2.405 ± 0.037
3.103AspThr: 3.103 ± 0.047
4.256AspVal: 4.256 ± 0.049
0.995AspTrp: 0.995 ± 0.023
1.055AspTyr: 1.055 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.316GluAla: 7.316 ± 0.086
0.383GluCys: 0.383 ± 0.015
2.674GluAsp: 2.674 ± 0.045
3.479GluGlu: 3.479 ± 0.058
1.418GluPhe: 1.418 ± 0.03
4.15GluGly: 4.15 ± 0.053
1.413GluHis: 1.413 ± 0.027
2.254GluIle: 2.254 ± 0.036
1.395GluLys: 1.395 ± 0.033
6.797GluLeu: 6.797 ± 0.077
0.897GluMet: 0.897 ± 0.023
0.941GluAsn: 0.941 ± 0.02
3.184GluPro: 3.184 ± 0.048
2.256GluGln: 2.256 ± 0.036
5.444GluArg: 5.444 ± 0.055
2.49GluSer: 2.49 ± 0.041
2.731GluThr: 2.731 ± 0.04
4.466GluVal: 4.466 ± 0.051
0.754GluTrp: 0.754 ± 0.021
1.007GluTyr: 1.007 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.725PheAla: 3.725 ± 0.053
0.277PheCys: 0.277 ± 0.012
1.888PheAsp: 1.888 ± 0.034
1.32PheGlu: 1.32 ± 0.025
0.877PhePhe: 0.877 ± 0.025
2.956PheGly: 2.956 ± 0.041
0.659PheHis: 0.659 ± 0.022
0.725PheIle: 0.725 ± 0.02
0.51PheLys: 0.51 ± 0.016
2.68PheLeu: 2.68 ± 0.047
0.388PheMet: 0.388 ± 0.016
0.506PheAsn: 0.506 ± 0.018
1.392PhePro: 1.392 ± 0.028
0.664PheGln: 0.664 ± 0.018
1.871PheArg: 1.871 ± 0.031
1.474PheSer: 1.474 ± 0.027
1.97PheThr: 1.97 ± 0.035
2.075PheVal: 2.075 ± 0.039
0.416PheTrp: 0.416 ± 0.016
0.543PheTyr: 0.543 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.772GlyAla: 11.772 ± 0.105
0.876GlyCys: 0.876 ± 0.022
4.911GlyAsp: 4.911 ± 0.044
4.995GlyGlu: 4.995 ± 0.051
2.839GlyPhe: 2.839 ± 0.04
9.095GlyGly: 9.095 ± 0.114
2.43GlyHis: 2.43 ± 0.035
3.517GlyIle: 3.517 ± 0.048
2.61GlyLys: 2.61 ± 0.053
9.482GlyLeu: 9.482 ± 0.08
2.093GlyMet: 2.093 ± 0.033
1.731GlyAsn: 1.731 ± 0.033
5.629GlyPro: 5.629 ± 0.069
2.74GlyGln: 2.74 ± 0.041
8.012GlyArg: 8.012 ± 0.081
5.335GlySer: 5.335 ± 0.06
6.275GlyThr: 6.275 ± 0.082
7.212GlyVal: 7.212 ± 0.062
1.658GlyTrp: 1.658 ± 0.031
2.228GlyTyr: 2.228 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.736HisAla: 2.736 ± 0.039
0.261HisCys: 0.261 ± 0.013
1.42HisAsp: 1.42 ± 0.03
1.214HisGlu: 1.214 ± 0.026
0.68HisPhe: 0.68 ± 0.02
2.618HisGly: 2.618 ± 0.038
0.768HisHis: 0.768 ± 0.023
0.688HisIle: 0.688 ± 0.022
0.401HisLys: 0.401 ± 0.014
2.583HisLeu: 2.583 ± 0.04
0.339HisMet: 0.339 ± 0.014
0.398HisAsn: 0.398 ± 0.014
1.937HisPro: 1.937 ± 0.033
0.723HisGln: 0.723 ± 0.021
2.278HisArg: 2.278 ± 0.035
1.11HisSer: 1.11 ± 0.026
1.454HisThr: 1.454 ± 0.029
1.613HisVal: 1.613 ± 0.028
0.382HisTrp: 0.382 ± 0.015
0.501HisTyr: 0.501 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.796IleAla: 4.796 ± 0.052
0.315IleCys: 0.315 ± 0.014
2.191IleAsp: 2.191 ± 0.041
2.011IleGlu: 2.011 ± 0.034
0.733IlePhe: 0.733 ± 0.019
3.595IleGly: 3.595 ± 0.053
0.622IleHis: 0.622 ± 0.018
0.903IleIle: 0.903 ± 0.023
0.788IleLys: 0.788 ± 0.022
2.319IleLeu: 2.319 ± 0.038
0.447IleMet: 0.447 ± 0.016
0.754IleAsn: 0.754 ± 0.022
1.765IlePro: 1.765 ± 0.031
0.761IleGln: 0.761 ± 0.021
2.204IleArg: 2.204 ± 0.037
1.674IleSer: 1.674 ± 0.03
2.135IleThr: 2.135 ± 0.04
2.661IleVal: 2.661 ± 0.046
0.36IleTrp: 0.36 ± 0.015
0.532IleTyr: 0.532 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
2.996LysAla: 2.996 ± 0.053
0.123LysCys: 0.123 ± 0.008
1.422LysAsp: 1.422 ± 0.039
1.245LysGlu: 1.245 ± 0.029
0.469LysPhe: 0.469 ± 0.019
1.945LysGly: 1.945 ± 0.044
0.472LysHis: 0.472 ± 0.017
0.932LysIle: 0.932 ± 0.024
1.02LysLys: 1.02 ± 0.038
2.055LysLeu: 2.055 ± 0.036
0.426LysMet: 0.426 ± 0.017
0.565LysAsn: 0.565 ± 0.022
1.385LysPro: 1.385 ± 0.031
0.741LysGln: 0.741 ± 0.022
1.518LysArg: 1.518 ± 0.032
1.19LysSer: 1.19 ± 0.029
1.329LysThr: 1.329 ± 0.031
1.875LysVal: 1.875 ± 0.043
0.261LysTrp: 0.261 ± 0.013
0.467LysTyr: 0.467 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
15.508LeuAla: 15.508 ± 0.116
0.936LeuCys: 0.936 ± 0.021
6.619LeuAsp: 6.619 ± 0.071
4.502LeuGlu: 4.502 ± 0.058
2.602LeuPhe: 2.602 ± 0.044
9.211LeuGly: 9.211 ± 0.092
2.477LeuHis: 2.477 ± 0.037
3.355LeuIle: 3.355 ± 0.05
2.026LeuLys: 2.026 ± 0.039
11.532LeuLeu: 11.532 ± 0.114
1.677LeuMet: 1.677 ± 0.035
1.652LeuAsn: 1.652 ± 0.027
6.784LeuPro: 6.784 ± 0.059
2.416LeuGln: 2.416 ± 0.04
8.869LeuArg: 8.869 ± 0.081
5.347LeuSer: 5.347 ± 0.055
7.003LeuThr: 7.003 ± 0.06
8.533LeuVal: 8.533 ± 0.079
1.286LeuTrp: 1.286 ± 0.031
1.8LeuTyr: 1.8 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
2.376MetAla: 2.376 ± 0.039
0.144MetCys: 0.144 ± 0.008
0.934MetAsp: 0.934 ± 0.022
0.748MetGlu: 0.748 ± 0.017
0.434MetPhe: 0.434 ± 0.014
1.346MetGly: 1.346 ± 0.028
0.371MetHis: 0.371 ± 0.013
0.668MetIle: 0.668 ± 0.021
0.427MetLys: 0.427 ± 0.014
1.738MetLeu: 1.738 ± 0.032
0.313MetMet: 0.313 ± 0.013
0.452MetAsn: 0.452 ± 0.016
1.156MetPro: 1.156 ± 0.026
0.475MetGln: 0.475 ± 0.017
1.368MetArg: 1.368 ± 0.029
1.286MetSer: 1.286 ± 0.026
1.469MetThr: 1.469 ± 0.027
1.31MetVal: 1.31 ± 0.025
0.232MetTrp: 0.232 ± 0.01
0.291MetTyr: 0.291 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.159AsnAla: 2.159 ± 0.039
0.179AsnCys: 0.179 ± 0.009
0.956AsnAsp: 0.956 ± 0.022
0.798AsnGlu: 0.798 ± 0.023
0.458AsnPhe: 0.458 ± 0.017
1.814AsnGly: 1.814 ± 0.033
0.394AsnHis: 0.394 ± 0.014
0.629AsnIle: 0.629 ± 0.021
0.446AsnLys: 0.446 ± 0.015
1.597AsnLeu: 1.597 ± 0.03
0.287AsnMet: 0.287 ± 0.014
0.402AsnAsn: 0.402 ± 0.019
1.262AsnPro: 1.262 ± 0.029
0.484AsnGln: 0.484 ± 0.021
1.23AsnArg: 1.23 ± 0.028
0.951AsnSer: 0.951 ± 0.023
1.1AsnThr: 1.1 ± 0.034
1.288AsnVal: 1.288 ± 0.027
0.28AsnTrp: 0.28 ± 0.013
0.412AsnTyr: 0.412 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.244ProAla: 9.244 ± 0.091
0.392ProCys: 0.392 ± 0.015
4.607ProAsp: 4.607 ± 0.062
4.296ProGlu: 4.296 ± 0.054
1.529ProPhe: 1.529 ± 0.029
6.844ProGly: 6.844 ± 0.077
1.572ProHis: 1.572 ± 0.029
1.187ProIle: 1.187 ± 0.029
1.255ProLys: 1.255 ± 0.03
5.564ProLeu: 5.564 ± 0.056
1.004ProMet: 1.004 ± 0.023
0.851ProAsn: 0.851 ± 0.023
3.795ProPro: 3.795 ± 0.071
2.088ProGln: 2.088 ± 0.056
4.18ProArg: 4.18 ± 0.055
3.141ProSer: 3.141 ± 0.049
3.115ProThr: 3.115 ± 0.04
5.574ProVal: 5.574 ± 0.06
0.909ProTrp: 0.909 ± 0.021
1.396ProTyr: 1.396 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.877GlnAla: 3.877 ± 0.051
0.191GlnCys: 0.191 ± 0.01
1.522GlnAsp: 1.522 ± 0.034
1.624GlnGlu: 1.624 ± 0.03
0.683GlnPhe: 0.683 ± 0.02
2.411GlnGly: 2.411 ± 0.044
0.775GlnHis: 0.775 ± 0.023
1.09GlnIle: 1.09 ± 0.025
0.664GlnLys: 0.664 ± 0.022
3.435GlnLeu: 3.435 ± 0.051
0.505GlnMet: 0.505 ± 0.019
0.511GlnAsn: 0.511 ± 0.02
1.83GlnPro: 1.83 ± 0.047
1.529GlnGln: 1.529 ± 0.074
2.543GlnArg: 2.543 ± 0.037
1.273GlnSer: 1.273 ± 0.028
1.337GlnThr: 1.337 ± 0.025
2.342GlnVal: 2.342 ± 0.033
0.489GlnTrp: 0.489 ± 0.017
0.56GlnTyr: 0.56 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.287ArgAla: 10.287 ± 0.096
0.641ArgCys: 0.641 ± 0.017
4.226ArgAsp: 4.226 ± 0.051
4.585ArgGlu: 4.585 ± 0.062
2.311ArgPhe: 2.311 ± 0.037
5.983ArgGly: 5.983 ± 0.063
2.204ArgHis: 2.204 ± 0.033
3.312ArgIle: 3.312 ± 0.043
1.784ArgLys: 1.784 ± 0.041
8.806ArgLeu: 8.806 ± 0.088
1.835ArgMet: 1.835 ± 0.033
1.334ArgAsn: 1.334 ± 0.028
5.305ArgPro: 5.305 ± 0.063
2.545ArgGln: 2.545 ± 0.04
8.052ArgArg: 8.052 ± 0.081
4.082ArgSer: 4.082 ± 0.053
5.695ArgThr: 5.695 ± 0.061
5.423ArgVal: 5.423 ± 0.057
1.397ArgTrp: 1.397 ± 0.031
1.822ArgTyr: 1.822 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.773SerAla: 6.773 ± 0.067
0.445SerCys: 0.445 ± 0.017
2.54SerAsp: 2.54 ± 0.038
2.289SerGlu: 2.289 ± 0.036
1.55SerPhe: 1.55 ± 0.027
5.908SerGly: 5.908 ± 0.061
1.074SerHis: 1.074 ± 0.023
1.382SerIle: 1.382 ± 0.025
1.073SerLys: 1.073 ± 0.024
4.697SerLeu: 4.697 ± 0.048
1.018SerMet: 1.018 ± 0.02
0.843SerAsn: 0.843 ± 0.026
3.174SerPro: 3.174 ± 0.047
1.287SerGln: 1.287 ± 0.024
3.687SerArg: 3.687 ± 0.042
2.765SerSer: 2.765 ± 0.051
2.83SerThr: 2.83 ± 0.046
4.012SerVal: 4.012 ± 0.048
0.91SerTrp: 0.91 ± 0.023
1.215SerTyr: 1.215 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
9.073ThrAla: 9.073 ± 0.077
0.45ThrCys: 0.45 ± 0.016
3.476ThrAsp: 3.476 ± 0.053
3.144ThrGlu: 3.144 ± 0.043
1.593ThrPhe: 1.593 ± 0.035
6.697ThrGly: 6.697 ± 0.069
1.223ThrHis: 1.223 ± 0.026
1.678ThrIle: 1.678 ± 0.037
1.204ThrLys: 1.204 ± 0.026
5.744ThrLeu: 5.744 ± 0.058
0.964ThrMet: 0.964 ± 0.024
0.979ThrAsn: 0.979 ± 0.027
4.101ThrPro: 4.101 ± 0.055
1.335ThrGln: 1.335 ± 0.027
3.866ThrArg: 3.866 ± 0.051
3.056ThrSer: 3.056 ± 0.046
3.804ThrThr: 3.804 ± 0.058
6.052ThrVal: 6.052 ± 0.078
0.845ThrTrp: 0.845 ± 0.022
1.228ThrTyr: 1.228 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.314ValAla: 10.314 ± 0.096
0.831ValCys: 0.831 ± 0.023
4.505ValAsp: 4.505 ± 0.056
4.398ValGlu: 4.398 ± 0.046
2.273ValPhe: 2.273 ± 0.038
6.247ValGly: 6.247 ± 0.067
2.061ValHis: 2.061 ± 0.035
2.909ValIle: 2.909 ± 0.047
1.674ValLys: 1.674 ± 0.038
9.439ValLeu: 9.439 ± 0.081
1.369ValMet: 1.369 ± 0.03
1.584ValAsn: 1.584 ± 0.032
5.212ValPro: 5.212 ± 0.055
2.148ValGln: 2.148 ± 0.031
6.973ValArg: 6.973 ± 0.069
4.109ValSer: 4.109 ± 0.047
5.361ValThr: 5.361 ± 0.06
7.366ValVal: 7.366 ± 0.074
1.077ValTrp: 1.077 ± 0.025
1.465ValTyr: 1.465 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.636TrpAla: 1.636 ± 0.03
0.172TrpCys: 0.172 ± 0.009
0.757TrpAsp: 0.757 ± 0.022
0.698TrpGlu: 0.698 ± 0.02
0.509TrpPhe: 0.509 ± 0.015
1.026TrpGly: 1.026 ± 0.028
0.398TrpHis: 0.398 ± 0.015
0.528TrpIle: 0.528 ± 0.017
0.372TrpLys: 0.372 ± 0.016
1.802TrpLeu: 1.802 ± 0.033
0.305TrpMet: 0.305 ± 0.013
0.35TrpAsn: 0.35 ± 0.014
0.762TrpPro: 0.762 ± 0.023
0.662TrpGln: 0.662 ± 0.019
1.295TrpArg: 1.295 ± 0.027
0.905TrpSer: 0.905 ± 0.024
0.979TrpThr: 0.979 ± 0.024
0.947TrpVal: 0.947 ± 0.024
0.335TrpTrp: 0.335 ± 0.013
0.34TrpTyr: 0.34 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.72TyrAla: 2.72 ± 0.04
0.179TyrCys: 0.179 ± 0.01
1.427TyrAsp: 1.427 ± 0.031
1.186TyrGlu: 1.186 ± 0.026
0.625TyrPhe: 0.625 ± 0.018
2.26TyrGly: 2.26 ± 0.04
0.395TyrHis: 0.395 ± 0.015
0.462TyrIle: 0.462 ± 0.019
0.384TyrLys: 0.384 ± 0.016
2.122TyrLeu: 2.122 ± 0.034
0.223TyrMet: 0.223 ± 0.012
0.346TyrAsn: 0.346 ± 0.013
1.074TyrPro: 1.074 ± 0.024
0.62TyrGln: 0.62 ± 0.017
1.829TyrArg: 1.829 ± 0.035
0.886TyrSer: 0.886 ± 0.021
1.126TyrThr: 1.126 ± 0.024
1.496TyrVal: 1.496 ± 0.026
0.325TyrTrp: 0.325 ± 0.014
0.42TyrTyr: 0.42 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.001XaaMet: 0.001 ± 0.001
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5851 proteins (1906603 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski