Chloroherpeton thalassium (strain ATCC 35110 / GB-78)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chloroherpeton; Chloroherpeton thalassium

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2708 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3QVI0|B3QVI0_CHLT3 Metallophosphoesterase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=Ctha_2128 PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84EE3 pKa = 4.57KK4 pKa = 10.9SITWKK9 pKa = 10.59NAVLKK14 pKa = 10.63VLSLLLIFAFAGCDD28 pKa = 4.13DD29 pKa = 5.39DD30 pKa = 5.94DD31 pKa = 6.21CPTQEE36 pKa = 5.46CSTTTCAGADD46 pKa = 3.34AALVLSEE53 pKa = 5.38GNYY56 pKa = 9.95EE57 pKa = 4.58SNNSTLTHH65 pKa = 6.68YY66 pKa = 11.34DD67 pKa = 2.97MEE69 pKa = 5.15TNVATQNYY77 pKa = 7.76FEE79 pKa = 4.02QRR81 pKa = 11.84IGRR84 pKa = 11.84LLGDD88 pKa = 4.14TGNDD92 pKa = 3.3MILVDD97 pKa = 4.64NILYY101 pKa = 10.17IVVNNSNKK109 pKa = 10.35LEE111 pKa = 4.12VVDD114 pKa = 3.81TDD116 pKa = 3.1TWTSLAKK123 pKa = 9.11ITLEE127 pKa = 3.95KK128 pKa = 10.76DD129 pKa = 3.19EE130 pKa = 5.2SGSSPRR136 pKa = 11.84EE137 pKa = 3.45IVEE140 pKa = 4.06YY141 pKa = 10.42NGKK144 pKa = 9.68LFISNFNGYY153 pKa = 8.8VAVVDD158 pKa = 3.95TANSYY163 pKa = 11.1GNEE166 pKa = 4.01IEE168 pKa = 4.27WIQVGTQPNGITVLDD183 pKa = 3.97GKK185 pKa = 10.8IYY187 pKa = 10.34VANSNYY193 pKa = 10.55DD194 pKa = 3.03NGYY197 pKa = 8.55NPGSISVIDD206 pKa = 3.9PTTLTVTQTLSDD218 pKa = 3.07IGVNISSIATDD229 pKa = 4.1DD230 pKa = 3.82YY231 pKa = 11.44GDD233 pKa = 4.56LYY235 pKa = 11.36VISKK239 pKa = 10.68GNYY242 pKa = 8.32SDD244 pKa = 5.26ASANLYY250 pKa = 8.99VVNPTTGAITKK261 pKa = 8.73TFDD264 pKa = 2.63ITAQSIAIHH273 pKa = 6.52GDD275 pKa = 2.92VGYY278 pKa = 10.95VSVGGYY284 pKa = 8.97DD285 pKa = 4.02ANWNYY290 pKa = 9.93VASIYY295 pKa = 10.69KK296 pKa = 9.7IDD298 pKa = 3.83VEE300 pKa = 4.67ADD302 pKa = 3.14TVMTEE307 pKa = 4.71DD308 pKa = 5.24FIEE311 pKa = 4.38SSNFQTLYY319 pKa = 10.99GLNVDD324 pKa = 4.6PNTGDD329 pKa = 3.55IYY331 pKa = 11.4CLDD334 pKa = 3.91ALDD337 pKa = 4.31YY338 pKa = 10.66SVSGTVTVFDD348 pKa = 4.68EE349 pKa = 5.77DD350 pKa = 4.16GQQTTSFKK358 pKa = 11.16SGVNPCKK365 pKa = 10.73VLFLEE370 pKa = 5.13DD371 pKa = 3.16

Molecular weight: 40.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3QYW1|B3QYW1_CHLT3 Redox-active disulfide protein 2 OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=Ctha_1190 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.06QPHH8 pKa = 4.63NRR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.06HH16 pKa = 3.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.25VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84HH40 pKa = 5.33RR41 pKa = 11.84LTVSSEE47 pKa = 4.03TSNKK51 pKa = 9.41KK52 pKa = 10.03

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2708

0

2708

947848

36

3944

350.0

39.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.114 ± 0.047

0.898 ± 0.017

5.218 ± 0.039

7.159 ± 0.05

4.958 ± 0.033

6.62 ± 0.045

2.031 ± 0.024

6.889 ± 0.042

6.569 ± 0.049

9.93 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.022

4.306 ± 0.033

3.82 ± 0.027

3.602 ± 0.029

4.505 ± 0.035

6.813 ± 0.051

5.272 ± 0.045

6.509 ± 0.035

0.983 ± 0.017

3.471 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski