Deinococcus deserti (strain DSM 17065 / CIP 109153 / LMG 22923 / VCD115)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3459 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1D3P1|C1D3P1_DEIDV Uncharacterized protein OS=Deinococcus deserti (strain DSM 17065 / CIP 109153 / LMG 22923 / VCD115) OX=546414 GN=Deide_3p01740 PE=4 SV=1
MM1 pKa = 7.87 AKK3 pKa = 8.89 GTLDD7 pKa = 3.2 TSRR10 pKa = 11.84 IRR12 pKa = 11.84 DD13 pKa = 3.33 WEE15 pKa = 4.35 TFHH18 pKa = 7.15 DD19 pKa = 3.87 VSMQAFGFPDD29 pKa = 4.69 FYY31 pKa = 11.12 GRR33 pKa = 11.84 NMDD36 pKa = 3.52 AWIDD40 pKa = 3.66 CLTYY44 pKa = 10.6 LDD46 pKa = 4.81 EE47 pKa = 6.3 ADD49 pKa = 3.85 GMSSVVLGSDD59 pKa = 3.55 EE60 pKa = 4.57 LLCIHH65 pKa = 6.31 VPGFSIFAASLPDD78 pKa = 3.37 VANAFLACSCFHH90 pKa = 6.02 QPAVYY95 pKa = 8.5 RR96 pKa = 11.84 TEE98 pKa = 4.27 GCFPTDD104 pKa = 3.58 FDD106 pKa = 4.14 AA107 pKa = 6.82
Molecular weight: 11.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 4.037
IPC_protein 3.999
Toseland 3.783
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.973
Rodwell 3.834
Grimsley 3.694
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.859
EMBOSS 3.986
Sillero 4.126
Patrickios 0.655
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 3.984
Protein with the highest isoelectric point:
>tr|C1CV97|C1CV97_DEIDV Putative DegV family protein OS=Deinococcus deserti (strain DSM 17065 / CIP 109153 / LMG 22923 / VCD115) OX=546414 GN=Deide_12040 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 4.81 SLGALAAQFGIANLFGRR19 pKa = 11.84 GFISVIITFFAAWVFTWQFIPRR41 pKa = 11.84 LRR43 pKa = 11.84 DD44 pKa = 3.26 FAVKK48 pKa = 10.32 AGWADD53 pKa = 3.35 QPNARR58 pKa = 11.84 RR59 pKa = 11.84 LNKK62 pKa = 10.16 EE63 pKa = 3.86 PLPNAGGLAIFAGFITSVVLAWALRR88 pKa = 11.84 PIMVEE93 pKa = 3.66 LVNIQVLSILLGASIMVLVGFIDD116 pKa = 3.86 DD117 pKa = 3.94 QFGLSPVTRR126 pKa = 11.84 LVVQVLVAVLLVVNGLKK143 pKa = 9.43 MDD145 pKa = 4.5 FNAIPFLPVLPEE157 pKa = 3.99 AVNEE161 pKa = 4.05 PLSTLLTIVWIVGLTNAVNLMDD183 pKa = 4.56 GVDD186 pKa = 3.82 GVVGGVGFVVSMVLLVTAAQFTDD209 pKa = 3.37 RR210 pKa = 11.84 AAAVILLAGLAGATLGYY227 pKa = 10.07 LRR229 pKa = 11.84 HH230 pKa = 6.18 NFNPSRR236 pKa = 11.84 IIMGDD241 pKa = 2.77 AGAYY245 pKa = 10.48 LIGFTLAAVSLLGTLKK261 pKa = 10.5 FSAGASLIVPLIVLALPVLDD281 pKa = 3.76 TTQVVIGRR289 pKa = 11.84 LARR292 pKa = 11.84 GIRR295 pKa = 11.84 NPLGHH300 pKa = 7.52 PDD302 pKa = 3.16 KK303 pKa = 10.5 THH305 pKa = 4.92 IHH307 pKa = 5.91 HH308 pKa = 6.94 RR309 pKa = 11.84 VLARR313 pKa = 11.84 TASARR318 pKa = 11.84 RR319 pKa = 11.84 TAVILWMVALACGMLGMLLQGVPLLAIGLTGVLVLVCLWFVAHH362 pKa = 6.21 RR363 pKa = 11.84 RR364 pKa = 11.84 VRR366 pKa = 11.84 AHH368 pKa = 5.87 SRR370 pKa = 11.84 EE371 pKa = 4.12 DD372 pKa = 3.25 RR373 pKa = 11.84 PAAAPEE379 pKa = 4.16 SHH381 pKa = 5.89 STRR384 pKa = 11.84 PP385 pKa = 3.3
Molecular weight: 41.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.414
IPC_protein 10.379
Toseland 10.672
ProMoST 10.496
Dawson 10.745
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.76
Grimsley 10.789
Solomon 10.95
Lehninger 10.906
Nozaki 10.672
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.096
Sillero 10.687
Patrickios 10.526
IPC_peptide 10.95
IPC2_peptide 9.692
IPC2.peptide.svr19 8.716
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3459
0
3459
1081085
10
3511
312.5
33.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.87 ± 0.059
0.616 ± 0.011
5.063 ± 0.032
5.596 ± 0.05
3.155 ± 0.024
8.769 ± 0.046
2.331 ± 0.024
3.687 ± 0.036
2.49 ± 0.035
11.513 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.081 ± 0.02
2.561 ± 0.03
5.646 ± 0.036
4.069 ± 0.029
7.327 ± 0.044
5.397 ± 0.03
6.159 ± 0.044
7.993 ± 0.035
1.349 ± 0.019
2.329 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here