Corynebacterium genitalium ATCC 33030
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2216 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D7W9Y6|D7W9Y6_9CORY Hypoxanthine phosphoribosyltransferase OS=Corynebacterium genitalium ATCC 33030 OX=585529 GN=hpt PE=3 SV=1
MM1 pKa = 7.42 QSVQVTDD8 pKa = 3.71 VPNDD12 pKa = 3.33 AQLIDD17 pKa = 3.66 VRR19 pKa = 11.84 EE20 pKa = 4.14 PDD22 pKa = 3.72 EE23 pKa = 5.02 FADD26 pKa = 3.59 THH28 pKa = 6.3 AAGAVNLPLSNFAVLAQQIDD48 pKa = 3.89 TTEE51 pKa = 3.84 PVYY54 pKa = 10.16 VICRR58 pKa = 11.84 SGGRR62 pKa = 11.84 SVEE65 pKa = 3.58 ASQYY69 pKa = 10.51 LEE71 pKa = 4.52 EE72 pKa = 5.02 IFGADD77 pKa = 3.21 VYY79 pKa = 11.43 NVLGGTQAWVNAGLPTEE96 pKa = 4.2
Molecular weight: 10.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.541
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.012
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|D7W968|D7W968_9CORY Uncharacterized protein OS=Corynebacterium genitalium ATCC 33030 OX=585529 GN=HMPREF0291_10606 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.04 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 ARR15 pKa = 11.84 KK16 pKa = 8.59 HH17 pKa = 4.63 GFRR20 pKa = 11.84 TRR22 pKa = 11.84 MNTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVSARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.59 KK38 pKa = 10.64 GRR40 pKa = 11.84 SKK42 pKa = 11.16 LSAA45 pKa = 3.74
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2216
0
2216
705372
39
1585
318.3
34.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.574 ± 0.068
0.654 ± 0.015
6.48 ± 0.051
6.655 ± 0.06
3.294 ± 0.036
8.513 ± 0.048
2.043 ± 0.024
4.962 ± 0.035
3.114 ± 0.043
9.194 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.021
2.925 ± 0.032
5.255 ± 0.049
3.234 ± 0.033
6.143 ± 0.053
5.633 ± 0.034
6.162 ± 0.041
8.177 ± 0.053
1.394 ± 0.026
2.236 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here