Microbacterium phage PhillyPhilly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DF44|A0A514DF44_9CAUD Uncharacterized protein OS=Microbacterium phage PhillyPhilly OX=2591218 GN=82 PE=4 SV=1
MM1 pKa = 7.51SICEE5 pKa = 4.15NFEE8 pKa = 3.96KK9 pKa = 10.4TIGTMTLEE17 pKa = 3.89GSIYY21 pKa = 8.95EE22 pKa = 4.27DD23 pKa = 4.28GYY25 pKa = 11.53VVVDD29 pKa = 4.39LLDD32 pKa = 3.59QFGHH36 pKa = 6.21EE37 pKa = 4.53VATMDD42 pKa = 4.24GTAEE46 pKa = 4.39DD47 pKa = 4.65GNTAEE52 pKa = 4.69LRR54 pKa = 11.84AHH56 pKa = 6.93IEE58 pKa = 3.6NALIDD63 pKa = 3.67EE64 pKa = 5.37HH65 pKa = 8.47YY66 pKa = 11.37NEE68 pKa = 4.5

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DEY5|A0A514DEY5_9CAUD Uncharacterized protein OS=Microbacterium phage PhillyPhilly OX=2591218 GN=19 PE=4 SV=1
MM1 pKa = 7.56SVIEE5 pKa = 4.32FKK7 pKa = 10.75NARR10 pKa = 11.84GQVRR14 pKa = 11.84YY15 pKa = 9.77QPALPDD21 pKa = 3.34GRR23 pKa = 11.84RR24 pKa = 11.84LYY26 pKa = 11.13SGGEE30 pKa = 3.61WMLPADD36 pKa = 3.92VDD38 pKa = 3.15AWAYY42 pKa = 10.1QPSLYY47 pKa = 9.76RR48 pKa = 11.84SKK50 pKa = 10.51RR51 pKa = 11.84RR52 pKa = 11.84ASRR55 pKa = 11.84RR56 pKa = 11.84VSWLIAEE63 pKa = 4.69EE64 pKa = 4.38FNAAQNQFKK73 pKa = 10.56AVAKK77 pKa = 10.42

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

18883

37

1252

171.7

19.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.094 ± 0.383

0.424 ± 0.076

6.636 ± 0.219

7.34 ± 0.327

3.389 ± 0.128

7.981 ± 0.276

1.885 ± 0.151

5.37 ± 0.147

4.565 ± 0.256

7.128 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.425 ± 0.148

3.813 ± 0.231

4.464 ± 0.207

3.479 ± 0.153

6.614 ± 0.201

5.99 ± 0.245

6.148 ± 0.251

7.139 ± 0.178

1.991 ± 0.158

3.125 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski