Amino acid dipepetide frequency for Microbacterium phage PhillyPhilly

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.387AlaAla: 11.387 ± 1.079
0.477AlaCys: 0.477 ± 0.163
5.561AlaAsp: 5.561 ± 0.604
7.679AlaGlu: 7.679 ± 0.796
3.231AlaPhe: 3.231 ± 0.333
7.044AlaGly: 7.044 ± 0.916
1.748AlaHis: 1.748 ± 0.342
5.137AlaIle: 5.137 ± 0.488
4.925AlaLys: 4.925 ± 0.594
9.268AlaLeu: 9.268 ± 0.812
3.389AlaMet: 3.389 ± 0.461
4.131AlaAsn: 4.131 ± 0.51
3.389AlaPro: 3.389 ± 0.368
3.707AlaGln: 3.707 ± 0.484
6.726AlaArg: 6.726 ± 0.75
6.037AlaSer: 6.037 ± 0.655
6.514AlaThr: 6.514 ± 0.646
6.249AlaVal: 6.249 ± 0.525
2.012AlaTrp: 2.012 ± 0.321
2.33AlaTyr: 2.33 ± 0.354
0.0AlaXaa: 0.0 ± 0.0
Cys
0.212CysAla: 0.212 ± 0.105
0.0CysCys: 0.0 ± 0.0
0.212CysAsp: 0.212 ± 0.102
0.424CysGlu: 0.424 ± 0.17
0.318CysPhe: 0.318 ± 0.114
0.583CysGly: 0.583 ± 0.216
0.106CysHis: 0.106 ± 0.066
0.318CysIle: 0.318 ± 0.148
0.212CysLys: 0.212 ± 0.116
0.106CysLeu: 0.106 ± 0.069
0.053CysMet: 0.053 ± 0.052
0.053CysAsn: 0.053 ± 0.05
0.212CysPro: 0.212 ± 0.123
0.318CysGln: 0.318 ± 0.124
0.318CysArg: 0.318 ± 0.12
0.106CysSer: 0.106 ± 0.068
0.212CysThr: 0.212 ± 0.116
0.212CysVal: 0.212 ± 0.106
0.159CysTrp: 0.159 ± 0.1
0.106CysTyr: 0.106 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
6.779AspAla: 6.779 ± 0.719
0.477AspCys: 0.477 ± 0.195
4.661AspAsp: 4.661 ± 0.474
5.243AspGlu: 5.243 ± 0.527
2.171AspPhe: 2.171 ± 0.394
5.508AspGly: 5.508 ± 0.479
0.9AspHis: 0.9 ± 0.219
3.072AspIle: 3.072 ± 0.504
2.383AspLys: 2.383 ± 0.349
5.031AspLeu: 5.031 ± 0.505
1.907AspMet: 1.907 ± 0.365
1.96AspAsn: 1.96 ± 0.374
3.654AspPro: 3.654 ± 0.47
1.96AspGln: 1.96 ± 0.35
3.654AspArg: 3.654 ± 0.503
3.76AspSer: 3.76 ± 0.422
3.654AspThr: 3.654 ± 0.503
4.978AspVal: 4.978 ± 0.449
1.748AspTrp: 1.748 ± 0.245
2.86AspTyr: 2.86 ± 0.405
0.0AspXaa: 0.0 ± 0.0
Glu
6.302GluAla: 6.302 ± 0.58
0.424GluCys: 0.424 ± 0.156
4.872GluAsp: 4.872 ± 0.528
4.978GluGlu: 4.978 ± 0.61
2.542GluPhe: 2.542 ± 0.364
4.766GluGly: 4.766 ± 0.534
1.695GluHis: 1.695 ± 0.296
4.819GluIle: 4.819 ± 0.547
4.078GluLys: 4.078 ± 0.542
4.872GluLeu: 4.872 ± 0.505
2.171GluMet: 2.171 ± 0.432
2.807GluAsn: 2.807 ± 0.429
2.436GluPro: 2.436 ± 0.415
4.025GluGln: 4.025 ± 0.558
5.084GluArg: 5.084 ± 0.635
3.231GluSer: 3.231 ± 0.495
4.29GluThr: 4.29 ± 0.543
5.296GluVal: 5.296 ± 0.629
1.748GluTrp: 1.748 ± 0.274
2.966GluTyr: 2.966 ± 0.348
0.0GluXaa: 0.0 ± 0.0
Phe
3.231PheAla: 3.231 ± 0.386
0.159PheCys: 0.159 ± 0.099
2.754PheAsp: 2.754 ± 0.323
2.913PheGlu: 2.913 ± 0.49
0.583PhePhe: 0.583 ± 0.158
2.277PheGly: 2.277 ± 0.322
0.741PheHis: 0.741 ± 0.178
1.483PheIle: 1.483 ± 0.244
1.324PheLys: 1.324 ± 0.314
2.224PheLeu: 2.224 ± 0.381
1.218PheMet: 1.218 ± 0.245
1.483PheAsn: 1.483 ± 0.33
0.953PhePro: 0.953 ± 0.224
1.589PheGln: 1.589 ± 0.262
2.171PheArg: 2.171 ± 0.328
1.801PheSer: 1.801 ± 0.354
2.595PheThr: 2.595 ± 0.388
2.489PheVal: 2.489 ± 0.361
0.477PheTrp: 0.477 ± 0.14
1.43PheTyr: 1.43 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
6.673GlyAla: 6.673 ± 0.721
0.371GlyCys: 0.371 ± 0.125
5.508GlyAsp: 5.508 ± 0.494
5.932GlyGlu: 5.932 ± 0.526
3.495GlyPhe: 3.495 ± 0.447
6.461GlyGly: 6.461 ± 0.741
2.012GlyHis: 2.012 ± 0.332
4.29GlyIle: 4.29 ± 0.582
3.178GlyLys: 3.178 ± 0.411
5.932GlyLeu: 5.932 ± 0.712
2.065GlyMet: 2.065 ± 0.302
3.019GlyAsn: 3.019 ± 0.487
2.118GlyPro: 2.118 ± 0.301
2.807GlyGln: 2.807 ± 0.453
5.508GlyArg: 5.508 ± 0.575
4.29GlySer: 4.29 ± 0.555
5.455GlyThr: 5.455 ± 0.582
5.561GlyVal: 5.561 ± 0.543
2.224GlyTrp: 2.224 ± 0.409
2.913GlyTyr: 2.913 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
1.96HisAla: 1.96 ± 0.338
0.0HisCys: 0.0 ± 0.0
0.953HisAsp: 0.953 ± 0.182
1.377HisGlu: 1.377 ± 0.253
0.477HisPhe: 0.477 ± 0.139
1.43HisGly: 1.43 ± 0.374
0.371HisHis: 0.371 ± 0.121
1.324HisIle: 1.324 ± 0.259
1.112HisLys: 1.112 ± 0.246
1.43HisLeu: 1.43 ± 0.333
0.371HisMet: 0.371 ± 0.125
0.688HisAsn: 0.688 ± 0.158
1.377HisPro: 1.377 ± 0.311
0.477HisGln: 0.477 ± 0.171
0.953HisArg: 0.953 ± 0.211
1.112HisSer: 1.112 ± 0.222
1.589HisThr: 1.589 ± 0.296
1.059HisVal: 1.059 ± 0.212
0.265HisTrp: 0.265 ± 0.105
0.53HisTyr: 0.53 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
5.402IleAla: 5.402 ± 0.568
0.159IleCys: 0.159 ± 0.114
3.972IleAsp: 3.972 ± 0.486
5.031IleGlu: 5.031 ± 0.536
1.748IlePhe: 1.748 ± 0.334
3.866IleGly: 3.866 ± 0.39
1.324IleHis: 1.324 ± 0.295
2.171IleIle: 2.171 ± 0.41
2.542IleLys: 2.542 ± 0.431
3.972IleLeu: 3.972 ± 0.401
0.953IleMet: 0.953 ± 0.193
2.224IleAsn: 2.224 ± 0.402
2.489IlePro: 2.489 ± 0.443
2.33IleGln: 2.33 ± 0.371
4.396IleArg: 4.396 ± 0.501
2.807IleSer: 2.807 ± 0.418
3.231IleThr: 3.231 ± 0.352
3.178IleVal: 3.178 ± 0.357
0.741IleTrp: 0.741 ± 0.26
1.165IleTyr: 1.165 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
5.508LysAla: 5.508 ± 0.63
0.159LysCys: 0.159 ± 0.098
3.125LysAsp: 3.125 ± 0.572
2.595LysGlu: 2.595 ± 0.359
1.96LysPhe: 1.96 ± 0.34
3.231LysGly: 3.231 ± 0.442
0.9LysHis: 0.9 ± 0.221
2.807LysIle: 2.807 ± 0.387
2.913LysLys: 2.913 ± 0.38
3.76LysLeu: 3.76 ± 0.522
1.165LysMet: 1.165 ± 0.244
1.271LysAsn: 1.271 ± 0.27
1.748LysPro: 1.748 ± 0.406
1.324LysGln: 1.324 ± 0.258
3.284LysArg: 3.284 ± 0.613
2.595LysSer: 2.595 ± 0.437
2.277LysThr: 2.277 ± 0.399
3.442LysVal: 3.442 ± 0.49
0.53LysTrp: 0.53 ± 0.14
1.059LysTyr: 1.059 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
7.679LeuAla: 7.679 ± 0.729
0.159LeuCys: 0.159 ± 0.09
5.72LeuAsp: 5.72 ± 0.601
4.555LeuGlu: 4.555 ± 0.555
2.33LeuPhe: 2.33 ± 0.322
6.514LeuGly: 6.514 ± 0.637
1.059LeuHis: 1.059 ± 0.235
3.284LeuIle: 3.284 ± 0.466
3.389LeuLys: 3.389 ± 0.378
3.442LeuLeu: 3.442 ± 0.435
1.218LeuMet: 1.218 ± 0.276
2.595LeuAsn: 2.595 ± 0.448
4.978LeuPro: 4.978 ± 0.5
2.542LeuGln: 2.542 ± 0.468
4.872LeuArg: 4.872 ± 0.517
5.296LeuSer: 5.296 ± 0.472
3.866LeuThr: 3.866 ± 0.541
4.502LeuVal: 4.502 ± 0.447
1.324LeuTrp: 1.324 ± 0.305
1.96LeuTyr: 1.96 ± 0.318
0.0LeuXaa: 0.0 ± 0.0
Met
3.178MetAla: 3.178 ± 0.365
0.053MetCys: 0.053 ± 0.061
1.483MetAsp: 1.483 ± 0.303
1.271MetGlu: 1.271 ± 0.249
0.794MetPhe: 0.794 ± 0.245
1.854MetGly: 1.854 ± 0.269
0.847MetHis: 0.847 ± 0.231
0.953MetIle: 0.953 ± 0.263
1.218MetLys: 1.218 ± 0.283
0.9MetLeu: 0.9 ± 0.219
0.583MetMet: 0.583 ± 0.182
0.847MetAsn: 0.847 ± 0.209
1.112MetPro: 1.112 ± 0.26
0.741MetGln: 0.741 ± 0.218
1.377MetArg: 1.377 ± 0.274
3.019MetSer: 3.019 ± 0.478
2.012MetThr: 2.012 ± 0.328
1.218MetVal: 1.218 ± 0.278
0.371MetTrp: 0.371 ± 0.126
0.424MetTyr: 0.424 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
3.389AsnAla: 3.389 ± 0.364
0.0AsnCys: 0.0 ± 0.0
1.907AsnAsp: 1.907 ± 0.315
2.33AsnGlu: 2.33 ± 0.325
1.218AsnPhe: 1.218 ± 0.257
4.449AsnGly: 4.449 ± 0.5
0.741AsnHis: 0.741 ± 0.224
1.642AsnIle: 1.642 ± 0.323
1.165AsnLys: 1.165 ± 0.261
3.178AsnLeu: 3.178 ± 0.394
0.847AsnMet: 0.847 ± 0.208
1.748AsnAsn: 1.748 ± 0.358
2.542AsnPro: 2.542 ± 0.349
1.218AsnGln: 1.218 ± 0.286
2.542AsnArg: 2.542 ± 0.399
2.807AsnSer: 2.807 ± 0.444
2.012AsnThr: 2.012 ± 0.318
2.648AsnVal: 2.648 ± 0.325
0.583AsnTrp: 0.583 ± 0.149
1.165AsnTyr: 1.165 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
3.813ProAla: 3.813 ± 0.429
0.106ProCys: 0.106 ± 0.069
3.76ProAsp: 3.76 ± 0.518
4.449ProGlu: 4.449 ± 0.574
1.589ProPhe: 1.589 ± 0.303
4.078ProGly: 4.078 ± 0.506
0.847ProHis: 0.847 ± 0.192
2.33ProIle: 2.33 ± 0.356
2.171ProLys: 2.171 ± 0.351
2.86ProLeu: 2.86 ± 0.356
0.688ProMet: 0.688 ± 0.191
1.642ProAsn: 1.642 ± 0.31
1.801ProPro: 1.801 ± 0.411
1.589ProGln: 1.589 ± 0.343
1.854ProArg: 1.854 ± 0.396
2.86ProSer: 2.86 ± 0.401
2.913ProThr: 2.913 ± 0.403
3.442ProVal: 3.442 ± 0.439
0.741ProTrp: 0.741 ± 0.21
1.112ProTyr: 1.112 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
3.813GlnAla: 3.813 ± 0.543
0.212GlnCys: 0.212 ± 0.114
1.907GlnAsp: 1.907 ± 0.353
2.33GlnGlu: 2.33 ± 0.342
1.43GlnPhe: 1.43 ± 0.244
2.542GlnGly: 2.542 ± 0.291
0.318GlnHis: 0.318 ± 0.123
2.542GlnIle: 2.542 ± 0.375
1.801GlnLys: 1.801 ± 0.347
2.754GlnLeu: 2.754 ± 0.353
0.847GlnMet: 0.847 ± 0.193
1.218GlnAsn: 1.218 ± 0.23
1.324GlnPro: 1.324 ± 0.305
0.9GlnGln: 0.9 ± 0.175
1.907GlnArg: 1.907 ± 0.31
2.065GlnSer: 2.065 ± 0.32
1.642GlnThr: 1.642 ± 0.278
3.601GlnVal: 3.601 ± 0.413
0.741GlnTrp: 0.741 ± 0.227
0.9GlnTyr: 0.9 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
6.09ArgAla: 6.09 ± 0.581
0.159ArgCys: 0.159 ± 0.089
4.131ArgAsp: 4.131 ± 0.38
5.19ArgGlu: 5.19 ± 0.692
2.277ArgPhe: 2.277 ± 0.304
4.713ArgGly: 4.713 ± 0.431
1.324ArgHis: 1.324 ± 0.271
4.237ArgIle: 4.237 ± 0.404
3.072ArgLys: 3.072 ± 0.521
4.661ArgLeu: 4.661 ± 0.502
1.854ArgMet: 1.854 ± 0.402
2.754ArgAsn: 2.754 ± 0.469
2.913ArgPro: 2.913 ± 0.402
2.012ArgGln: 2.012 ± 0.358
4.978ArgArg: 4.978 ± 0.551
3.337ArgSer: 3.337 ± 0.456
3.231ArgThr: 3.231 ± 0.432
5.296ArgVal: 5.296 ± 0.602
1.377ArgTrp: 1.377 ± 0.283
2.542ArgTyr: 2.542 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
6.514SerAla: 6.514 ± 0.732
0.212SerCys: 0.212 ± 0.112
3.548SerAsp: 3.548 ± 0.474
3.442SerGlu: 3.442 ± 0.449
1.854SerPhe: 1.854 ± 0.296
5.455SerGly: 5.455 ± 0.604
1.271SerHis: 1.271 ± 0.309
3.125SerIle: 3.125 ± 0.422
3.178SerLys: 3.178 ± 0.461
4.872SerLeu: 4.872 ± 0.498
1.695SerMet: 1.695 ± 0.389
2.33SerAsn: 2.33 ± 0.478
2.277SerPro: 2.277 ± 0.376
1.43SerGln: 1.43 ± 0.256
3.442SerArg: 3.442 ± 0.401
3.866SerSer: 3.866 ± 0.594
4.025SerThr: 4.025 ± 0.549
3.548SerVal: 3.548 ± 0.442
1.483SerTrp: 1.483 ± 0.319
2.277SerTyr: 2.277 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
5.667ThrAla: 5.667 ± 0.649
0.318ThrCys: 0.318 ± 0.169
3.76ThrAsp: 3.76 ± 0.491
4.237ThrGlu: 4.237 ± 0.47
2.648ThrPhe: 2.648 ± 0.355
5.826ThrGly: 5.826 ± 0.605
0.847ThrHis: 0.847 ± 0.241
3.707ThrIle: 3.707 ± 0.435
2.277ThrLys: 2.277 ± 0.307
4.661ThrLeu: 4.661 ± 0.448
1.165ThrMet: 1.165 ± 0.26
2.33ThrAsn: 2.33 ± 0.37
3.707ThrPro: 3.707 ± 0.431
1.748ThrGln: 1.748 ± 0.234
3.601ThrArg: 3.601 ± 0.421
3.442ThrSer: 3.442 ± 0.428
3.707ThrThr: 3.707 ± 0.487
4.978ThrVal: 4.978 ± 0.563
1.059ThrTrp: 1.059 ± 0.239
1.801ThrTyr: 1.801 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
7.626ValAla: 7.626 ± 0.636
0.53ValCys: 0.53 ± 0.184
5.137ValAsp: 5.137 ± 0.587
5.561ValGlu: 5.561 ± 0.601
1.907ValPhe: 1.907 ± 0.317
5.084ValGly: 5.084 ± 0.507
1.059ValHis: 1.059 ± 0.212
4.025ValIle: 4.025 ± 0.52
3.601ValLys: 3.601 ± 0.478
4.608ValLeu: 4.608 ± 0.485
0.688ValMet: 0.688 ± 0.193
2.754ValAsn: 2.754 ± 0.407
3.284ValPro: 3.284 ± 0.426
2.224ValGln: 2.224 ± 0.281
5.508ValArg: 5.508 ± 0.431
3.919ValSer: 3.919 ± 0.429
5.19ValThr: 5.19 ± 0.725
5.19ValVal: 5.19 ± 0.493
1.324ValTrp: 1.324 ± 0.239
2.171ValTyr: 2.171 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
1.854TrpAla: 1.854 ± 0.336
0.106TrpCys: 0.106 ± 0.07
1.536TrpAsp: 1.536 ± 0.273
1.483TrpGlu: 1.483 ± 0.332
0.583TrpPhe: 0.583 ± 0.179
1.801TrpGly: 1.801 ± 0.263
0.265TrpHis: 0.265 ± 0.121
1.165TrpIle: 1.165 ± 0.224
0.318TrpLys: 0.318 ± 0.131
0.9TrpLeu: 0.9 ± 0.182
0.636TrpMet: 0.636 ± 0.183
1.112TrpAsn: 1.112 ± 0.196
0.847TrpPro: 0.847 ± 0.234
0.583TrpGln: 0.583 ± 0.207
1.748TrpArg: 1.748 ± 0.323
1.271TrpSer: 1.271 ± 0.329
0.9TrpThr: 0.9 ± 0.245
1.695TrpVal: 1.695 ± 0.304
0.583TrpTrp: 0.583 ± 0.146
0.53TrpTyr: 0.53 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.866TyrAla: 3.866 ± 0.542
0.159TyrCys: 0.159 ± 0.082
1.854TyrAsp: 1.854 ± 0.335
2.383TyrGlu: 2.383 ± 0.34
0.741TyrPhe: 0.741 ± 0.209
2.33TyrGly: 2.33 ± 0.388
0.477TyrHis: 0.477 ± 0.158
1.271TyrIle: 1.271 ± 0.246
0.794TyrLys: 0.794 ± 0.193
1.854TyrLeu: 1.854 ± 0.38
0.688TyrMet: 0.688 ± 0.163
1.165TyrAsn: 1.165 ± 0.221
1.483TyrPro: 1.483 ± 0.253
1.271TyrGln: 1.271 ± 0.273
2.224TyrArg: 2.224 ± 0.412
2.277TyrSer: 2.277 ± 0.358
2.171TyrThr: 2.171 ± 0.287
2.807TyrVal: 2.807 ± 0.384
0.424TyrTrp: 0.424 ± 0.145
1.006TyrTyr: 1.006 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 110 proteins (18883 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski