Bison bison bison

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29577 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P3GQ86|A0A6P3GQ86_BISBI melanoma-associated antigen 10-like OS=Bison bison bison OX=43346 GN=LOC104983845 PE=4 SV=1
GG1 pKa = 8.25DD2 pKa = 3.65IQQLLLVSDD11 pKa = 4.08HH12 pKa = 6.57RR13 pKa = 11.84AAYY16 pKa = 9.28DD17 pKa = 3.56YY18 pKa = 10.97CEE20 pKa = 5.09HH21 pKa = 6.88YY22 pKa = 11.21SPDD25 pKa = 3.94CDD27 pKa = 3.16TAVPDD32 pKa = 4.49KK33 pKa = 10.74PQSQDD38 pKa = 3.47PNPDD42 pKa = 3.48EE43 pKa = 5.17YY44 pKa = 11.27EE45 pKa = 3.96LTQPPTEE52 pKa = 4.55APSAPSTTEE61 pKa = 3.92GPGKK65 pKa = 10.61EE66 pKa = 4.09EE67 pKa = 4.34DD68 pKa = 4.2PGIGDD73 pKa = 3.71YY74 pKa = 10.99DD75 pKa = 3.92YY76 pKa = 11.74VPSEE80 pKa = 5.03DD81 pKa = 5.21YY82 pKa = 8.57YY83 pKa = 11.2TPPPYY88 pKa = 10.55EE89 pKa = 4.01DD90 pKa = 3.07FGYY93 pKa = 11.22GEE95 pKa = 5.12GIEE98 pKa = 4.87NPDD101 pKa = 4.02EE102 pKa = 5.22NPDD105 pKa = 3.58QLPDD109 pKa = 3.14QGARR113 pKa = 11.84AEE115 pKa = 4.54VPTSTVLTSNGSNPAPPPEE134 pKa = 4.28EE135 pKa = 5.77GIDD138 pKa = 3.7DD139 pKa = 4.1LEE141 pKa = 4.46EE142 pKa = 4.14EE143 pKa = 4.53FTEE146 pKa = 4.24EE147 pKa = 4.01TIKK150 pKa = 11.11NLDD153 pKa = 3.18EE154 pKa = 4.85SYY156 pKa = 11.05YY157 pKa = 11.07DD158 pKa = 3.68PYY160 pKa = 11.27YY161 pKa = 11.04DD162 pKa = 3.66PTISPSEE169 pKa = 4.13IGPGMPANQDD179 pKa = 3.51TIYY182 pKa = 10.65EE183 pKa = 4.36GMGGPRR189 pKa = 11.84GEE191 pKa = 4.5KK192 pKa = 9.01GQKK195 pKa = 9.93GEE197 pKa = 4.14PAIIEE202 pKa = 4.22PGMLLEE208 pKa = 5.04GPPGPEE214 pKa = 4.27GPAGLPGPPGTMGPTGQVGDD234 pKa = 4.16PGEE237 pKa = 4.51RR238 pKa = 11.84GPPGRR243 pKa = 11.84PGLPGADD250 pKa = 3.87GLPGPPGTMLMLPFRR265 pKa = 11.84FGGGGDD271 pKa = 3.7AGSKK275 pKa = 10.78GPMVSAQEE283 pKa = 4.2SQAQAILQQARR294 pKa = 11.84VSGLGHH300 pKa = 6.96GGRR303 pKa = 11.84LGAQLARR310 pKa = 11.84LLTWGPRR317 pKa = 11.84FPDD320 pKa = 2.94MDD322 pKa = 3.82MAAEE326 pKa = 4.18PLWLLLTPSSSTIYY340 pKa = 10.15CLPPGSGMDD349 pKa = 3.47RR350 pKa = 11.84PHH352 pKa = 7.14VLLGGPLL359 pKa = 3.29

Molecular weight: 37.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P3HRY6|A0A6P3HRY6_BISBI FERM and PDZ domain-containing protein 1 OS=Bison bison bison OX=43346 GN=FRMPD1 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20565

9012

29577

17611492

31

34175

595.4

66.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.815 ± 0.013

2.182 ± 0.011

4.839 ± 0.011

7.17 ± 0.02

3.627 ± 0.009

6.314 ± 0.019

2.578 ± 0.007

4.394 ± 0.011

5.872 ± 0.02

9.96 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.007

3.623 ± 0.009

6.23 ± 0.02

4.836 ± 0.013

5.634 ± 0.012

8.546 ± 0.017

5.343 ± 0.012

6.032 ± 0.011

1.187 ± 0.006

2.63 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski