Stentor coeruleus
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30969 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R2CIJ7|A0A1R2CIJ7_9CILI Uncharacterized protein OS=Stentor coeruleus OX=5963 GN=SteCoe_9126 PE=4 SV=1
MM1 pKa = 7.64 LIISLLLQVIGSLGAIEE18 pKa = 5.02 YY19 pKa = 7.38 FTKK22 pKa = 10.53 GQHH25 pKa = 5.83 LKK27 pKa = 10.69 FYY29 pKa = 10.18 FPTVDD34 pKa = 2.94 QVTFEE39 pKa = 4.14 LWVPSANLLGFDD51 pKa = 4.44 WIGIAIQCTNYY62 pKa = 9.43 PRR64 pKa = 11.84 NAFQADD70 pKa = 4.36 YY71 pKa = 9.78 YY72 pKa = 10.5 IAMVDD77 pKa = 4.57 DD78 pKa = 4.13 VFTDD82 pKa = 3.54 RR83 pKa = 11.84 WADD86 pKa = 3.36 GHH88 pKa = 5.95 YY89 pKa = 10.84 LPVLDD94 pKa = 4.69 TDD96 pKa = 4.43 LGGNSDD102 pKa = 3.61 IVSDD106 pKa = 3.78 RR107 pKa = 11.84 YY108 pKa = 10.42 EE109 pKa = 4.11 YY110 pKa = 10.77 EE111 pKa = 4.39 DD112 pKa = 3.71 YY113 pKa = 11.14 VIYY116 pKa = 10.18 TLTRR120 pKa = 11.84 SLATGDD126 pKa = 4.18 VYY128 pKa = 11.34 DD129 pKa = 3.97 QEE131 pKa = 5.38 LVVDD135 pKa = 4.5 TPYY138 pKa = 10.33 MIQWAKK144 pKa = 10.87 GNLVDD149 pKa = 5.44 GEE151 pKa = 4.56 IGVHH155 pKa = 5.76 TMDD158 pKa = 3.14 NCGFEE163 pKa = 4.24 YY164 pKa = 10.8 LILADD169 pKa = 5.35 DD170 pKa = 4.11 YY171 pKa = 11.83 LDD173 pKa = 5.22 RR174 pKa = 11.84 NHH176 pKa = 7.63 DD177 pKa = 4.11 DD178 pKa = 3.55 NGKK181 pKa = 9.9 FGPWTDD187 pKa = 3.24 QFRR190 pKa = 11.84 DD191 pKa = 4.5 FVRR194 pKa = 11.84 KK195 pKa = 8.64 EE196 pKa = 3.92 DD197 pKa = 3.97 KK198 pKa = 10.24 EE199 pKa = 4.24 ALVMWTDD206 pKa = 5.7 LIDD209 pKa = 4.32 EE210 pKa = 4.9 DD211 pKa = 5.82 DD212 pKa = 3.85 EE213 pKa = 4.99 LGNIEE218 pKa = 5.85 DD219 pKa = 4.73 IEE221 pKa = 5.35 DD222 pKa = 4.08 DD223 pKa = 3.79 FATDD227 pKa = 3.24 EE228 pKa = 5.54 DD229 pKa = 4.45 VVEE232 pKa = 4.81 DD233 pKa = 3.75 NN234 pKa = 4.81
Molecular weight: 26.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A1R2BMS3|A0A1R2BMS3_9CILI Uncharacterized protein OS=Stentor coeruleus OX=5963 GN=SteCoe_22191 PE=3 SV=1
MM1 pKa = 7.8 RR2 pKa = 11.84 KK3 pKa = 10.06 NIGFYY8 pKa = 10.28 DD9 pKa = 4.16 LCRR12 pKa = 11.84 LGKK15 pKa = 9.85 ISGMGMNDD23 pKa = 2.84 KK24 pKa = 10.96 GGSEE28 pKa = 4.44 DD29 pKa = 3.52 FWPLVLSNVLKK40 pKa = 10.55 RR41 pKa = 11.84 ARR43 pKa = 11.84 FRR45 pKa = 11.84 ILSWIRR51 pKa = 11.84 RR52 pKa = 11.84 KK53 pKa = 10.39 EE54 pKa = 3.73 MLRR57 pKa = 11.84 YY58 pKa = 9.11 NVNGCVEE65 pKa = 3.65 IGLRR69 pKa = 11.84 YY70 pKa = 9.75 ARR72 pKa = 4.52
Molecular weight: 8.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.692
IPC_protein 10.452
Toseland 10.599
ProMoST 10.57
Dawson 10.716
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.965
Grimsley 10.774
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.409
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.643
Patrickios 10.716
IPC_peptide 10.818
IPC2_peptide 9.502
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30969
0
30969
13122840
66
5139
423.7
48.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.322 ± 0.01
1.939 ± 0.014
5.065 ± 0.01
7.659 ± 0.022
4.833 ± 0.012
4.35 ± 0.015
1.854 ± 0.006
8.44 ± 0.012
9.245 ± 0.023
9.556 ± 0.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.006
6.267 ± 0.011
3.548 ± 0.012
3.667 ± 0.008
3.944 ± 0.011
8.307 ± 0.017
4.917 ± 0.013
4.898 ± 0.011
0.929 ± 0.004
3.974 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here