Amino acid dipepetide frequency for Stentor coeruleus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.051AlaAla: 2.051 ± 0.016	0.931AlaCys: 0.931 ± 0.012	1.977AlaAsp: 1.977 ± 0.015	3.171AlaGlu: 3.171 ± 0.021	2.222AlaPhe: 2.222 ± 0.017	2.032AlaGly: 2.032 ± 0.016	0.795AlaHis: 0.795 ± 0.009	3.442AlaIle: 3.442 ± 0.019	3.85AlaLys: 3.85 ± 0.022	4.825AlaLeu: 4.825 ± 0.026	1.069AlaMet: 1.069 ± 0.009	2.318AlaAsn: 2.318 ± 0.014	1.307AlaPro: 1.307 ± 0.013	1.548AlaGln: 1.548 ± 0.011	1.763AlaArg: 1.763 ± 0.014	3.336AlaSer: 3.336 ± 0.017	1.88AlaThr: 1.88 ± 0.014	2.421AlaVal: 2.421 ± 0.015	0.418AlaTrp: 0.418 ± 0.005	1.857AlaTyr: 1.857 ± 0.014	0.004AlaXaa: 0.004 ± 0.001
Cys	0.78CysAla: 0.78 ± 0.009	0.473CysCys: 0.473 ± 0.01	0.974CysAsp: 0.974 ± 0.014	1.372CysGlu: 1.372 ± 0.015	1.009CysPhe: 1.009 ± 0.011	0.978CysGly: 0.978 ± 0.012	0.31CysHis: 0.31 ± 0.005	1.601CysIle: 1.601 ± 0.016	1.727CysLys: 1.727 ± 0.018	1.964CysLeu: 1.964 ± 0.018	0.441CysMet: 0.441 ± 0.006	1.08CysAsn: 1.08 ± 0.016	0.761CysPro: 0.761 ± 0.015	0.673CysGln: 0.673 ± 0.009	0.737CysArg: 0.737 ± 0.009	1.62CysSer: 1.62 ± 0.021	0.964CysThr: 0.964 ± 0.019	0.967CysVal: 0.967 ± 0.01	0.175CysTrp: 0.175 ± 0.004	0.785CysTyr: 0.785 ± 0.01	0.002CysXaa: 0.002 ± 0.0
Asp	1.769AspAla: 1.769 ± 0.015	0.953AspCys: 0.953 ± 0.012	2.789AspAsp: 2.789 ± 0.024	3.918AspGlu: 3.918 ± 0.025	3.184AspPhe: 3.184 ± 0.016	2.085AspGly: 2.085 ± 0.02	0.909AspHis: 0.909 ± 0.01	4.598AspIle: 4.598 ± 0.021	4.286AspLys: 4.286 ± 0.021	5.445AspLeu: 5.445 ± 0.026	1.321AspMet: 1.321 ± 0.012	2.866AspAsn: 2.866 ± 0.015	1.802AspPro: 1.802 ± 0.011	1.598AspGln: 1.598 ± 0.011	1.79AspArg: 1.79 ± 0.014	3.924AspSer: 3.924 ± 0.022	2.272AspThr: 2.272 ± 0.016	2.192AspVal: 2.192 ± 0.013	0.529AspTrp: 0.529 ± 0.007	2.413AspTyr: 2.413 ± 0.015	0.005AspXaa: 0.005 ± 0.001
Glu	3.329GluAla: 3.329 ± 0.02	1.223GluCys: 1.223 ± 0.015	4.189GluAsp: 4.189 ± 0.023	7.107GluGlu: 7.107 ± 0.052	3.373GluPhe: 3.373 ± 0.016	2.931GluGly: 2.931 ± 0.018	1.174GluHis: 1.174 ± 0.013	7.327GluIle: 7.327 ± 0.029	8.539GluLys: 8.539 ± 0.047	6.852GluLeu: 6.852 ± 0.037	1.795GluMet: 1.795 ± 0.012	6.155GluAsn: 6.155 ± 0.034	1.677GluPro: 1.677 ± 0.015	2.304GluGln: 2.304 ± 0.017	2.846GluArg: 2.846 ± 0.023	5.197GluSer: 5.197 ± 0.027	3.388GluThr: 3.388 ± 0.02	3.825GluVal: 3.825 ± 0.021	0.598GluTrp: 0.598 ± 0.007	2.755GluTyr: 2.755 ± 0.014	0.006GluXaa: 0.006 ± 0.001
Phe	2.103PheAla: 2.103 ± 0.015	0.996PheCys: 0.996 ± 0.01	2.628PheAsp: 2.628 ± 0.015	3.274PheGlu: 3.274 ± 0.017	2.415PhePhe: 2.415 ± 0.018	2.314PheGly: 2.314 ± 0.015	0.884PheHis: 0.884 ± 0.008	4.112PheIle: 4.112 ± 0.022	3.693PheLys: 3.693 ± 0.02	4.693PheLeu: 4.693 ± 0.024	1.162PheMet: 1.162 ± 0.01	2.728PheAsn: 2.728 ± 0.018	1.697PhePro: 1.697 ± 0.013	1.646PheGln: 1.646 ± 0.011	1.86PheArg: 1.86 ± 0.014	4.457PheSer: 4.457 ± 0.021	2.73PheThr: 2.73 ± 0.016	2.262PheVal: 2.262 ± 0.016	0.48PheTrp: 0.48 ± 0.006	2.193PheTyr: 2.193 ± 0.016	0.004PheXaa: 0.004 ± 0.001
Gly	1.696GlyAla: 1.696 ± 0.017	0.956GlyCys: 0.956 ± 0.013	2.152GlyAsp: 2.152 ± 0.017	2.753GlyGlu: 2.753 ± 0.017	2.423GlyPhe: 2.423 ± 0.017	2.231GlyGly: 2.231 ± 0.025	1.043GlyHis: 1.043 ± 0.012	3.728GlyIle: 3.728 ± 0.02	4.118GlyLys: 4.118 ± 0.023	3.922GlyLeu: 3.922 ± 0.019	0.998GlyMet: 0.998 ± 0.011	2.67GlyAsn: 2.67 ± 0.017	1.218GlyPro: 1.218 ± 0.015	1.468GlyGln: 1.468 ± 0.012	1.703GlyArg: 1.703 ± 0.014	3.382GlySer: 3.382 ± 0.021	2.237GlyThr: 2.237 ± 0.02	2.438GlyVal: 2.438 ± 0.017	0.414GlyTrp: 0.414 ± 0.007	1.949GlyTyr: 1.949 ± 0.02	0.004GlyXaa: 0.004 ± 0.001
His	0.708HisAla: 0.708 ± 0.008	0.393HisCys: 0.393 ± 0.006	0.841HisAsp: 0.841 ± 0.008	1.363HisGlu: 1.363 ± 0.015	0.941HisPhe: 0.941 ± 0.009	0.847HisGly: 0.847 ± 0.011	0.431HisHis: 0.431 ± 0.011	1.514HisIle: 1.514 ± 0.011	1.689HisLys: 1.689 ± 0.012	1.796HisLeu: 1.796 ± 0.012	0.43HisMet: 0.43 ± 0.006	1.087HisAsn: 1.087 ± 0.009	0.831HisPro: 0.831 ± 0.008	0.633HisGln: 0.633 ± 0.007	0.86HisArg: 0.86 ± 0.009	1.548HisSer: 1.548 ± 0.012	0.942HisThr: 0.942 ± 0.009	0.762HisVal: 0.762 ± 0.008	0.177HisTrp: 0.177 ± 0.004	0.742HisTyr: 0.742 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	3.569IleAla: 3.569 ± 0.019	1.712IleCys: 1.712 ± 0.015	4.353IleAsp: 4.353 ± 0.021	6.688IleGlu: 6.688 ± 0.028	4.063IlePhe: 4.063 ± 0.023	3.564IleGly: 3.564 ± 0.019	1.484IleHis: 1.484 ± 0.011	6.941IleIle: 6.941 ± 0.032	7.732IleLys: 7.732 ± 0.031	7.859IleLeu: 7.859 ± 0.037	1.764IleMet: 1.764 ± 0.013	4.907IleAsn: 4.907 ± 0.024	3.23IlePro: 3.23 ± 0.019	3.292IleGln: 3.292 ± 0.017	3.32IleArg: 3.32 ± 0.017	7.589IleSer: 7.589 ± 0.028	4.089IleThr: 4.089 ± 0.021	4.188IleVal: 4.188 ± 0.022	0.876IleTrp: 0.876 ± 0.011	3.174IleTyr: 3.174 ± 0.02	0.006IleXaa: 0.006 ± 0.001
Lys	4.213LysAla: 4.213 ± 0.023	1.658LysCys: 1.658 ± 0.019	4.854LysAsp: 4.854 ± 0.026	7.04LysGlu: 7.04 ± 0.041	3.72LysPhe: 3.72 ± 0.019	3.393LysGly: 3.393 ± 0.021	1.799LysHis: 1.799 ± 0.013	8.448LysIle: 8.448 ± 0.034	9.482LysLys: 9.482 ± 0.047	8.111LysLeu: 8.111 ± 0.034	1.906LysMet: 1.906 ± 0.013	6.801LysAsn: 6.801 ± 0.032	3.141LysPro: 3.141 ± 0.023	3.121LysGln: 3.121 ± 0.02	3.638LysArg: 3.638 ± 0.024	7.406LysSer: 7.406 ± 0.031	4.899LysThr: 4.899 ± 0.021	4.582LysVal: 4.582 ± 0.021	0.698LysTrp: 0.698 ± 0.009	3.535LysTyr: 3.535 ± 0.018	0.008LysXaa: 0.008 ± 0.001
Leu	4.704LeuAla: 4.704 ± 0.024	1.841LeuCys: 1.841 ± 0.015	4.703LeuAsp: 4.703 ± 0.024	7.799LeuGlu: 7.799 ± 0.035	3.911LeuPhe: 3.911 ± 0.021	4.291LeuGly: 4.291 ± 0.021	1.822LeuHis: 1.822 ± 0.013	7.295LeuIle: 7.295 ± 0.031	9.416LeuLys: 9.416 ± 0.037	8.56LeuLeu: 8.56 ± 0.035	2.1LeuMet: 2.1 ± 0.012	5.927LeuAsn: 5.927 ± 0.023	3.344LeuPro: 3.344 ± 0.017	3.98LeuGln: 3.98 ± 0.022	4.0LeuArg: 4.0 ± 0.021	8.208LeuSer: 8.208 ± 0.03	4.793LeuThr: 4.793 ± 0.022	4.513LeuVal: 4.513 ± 0.021	0.86LeuTrp: 0.86 ± 0.01	3.486LeuTyr: 3.486 ± 0.02	0.009LeuXaa: 0.009 ± 0.001
Met	1.057MetAla: 1.057 ± 0.009	0.389MetCys: 0.389 ± 0.006	1.078MetAsp: 1.078 ± 0.009	1.622MetGlu: 1.622 ± 0.013	0.99MetPhe: 0.99 ± 0.009	0.963MetGly: 0.963 ± 0.009	0.522MetHis: 0.522 ± 0.006	2.037MetIle: 2.037 ± 0.013	2.239MetLys: 2.239 ± 0.013	2.156MetLeu: 2.156 ± 0.016	0.613MetMet: 0.613 ± 0.009	1.515MetAsn: 1.515 ± 0.012	0.821MetPro: 0.821 ± 0.011	1.011MetGln: 1.011 ± 0.01	0.944MetArg: 0.944 ± 0.01	1.802MetSer: 1.802 ± 0.012	1.055MetThr: 1.055 ± 0.01	1.003MetVal: 1.003 ± 0.01	0.236MetTrp: 0.236 ± 0.004	0.712MetTyr: 0.712 ± 0.007	0.002MetXaa: 0.002 ± 0.0
Asn	2.365AsnAla: 2.365 ± 0.013	1.292AsnCys: 1.292 ± 0.017	3.153AsnAsp: 3.153 ± 0.016	4.662AsnGlu: 4.662 ± 0.025	3.407AsnPhe: 3.407 ± 0.016	2.195AsnGly: 2.195 ± 0.017	1.201AsnHis: 1.201 ± 0.011	5.419AsnIle: 5.419 ± 0.023	5.248AsnLys: 5.248 ± 0.023	6.357AsnLeu: 6.357 ± 0.024	1.493AsnMet: 1.493 ± 0.012	3.748AsnAsn: 3.748 ± 0.02	2.726AsnPro: 2.726 ± 0.022	2.365AsnGln: 2.365 ± 0.016	2.231AsnArg: 2.231 ± 0.014	5.64AsnSer: 5.64 ± 0.024	3.359AsnThr: 3.359 ± 0.018	2.407AsnVal: 2.407 ± 0.015	0.578AsnTrp: 0.578 ± 0.007	2.82AsnTyr: 2.82 ± 0.017	0.005AsnXaa: 0.005 ± 0.001
Pro	1.398ProAla: 1.398 ± 0.011	0.576ProCys: 0.576 ± 0.01	1.819ProAsp: 1.819 ± 0.013	3.019ProGlu: 3.019 ± 0.019	1.49ProPhe: 1.49 ± 0.013	1.78ProGly: 1.78 ± 0.022	0.6ProHis: 0.6 ± 0.007	2.773ProIle: 2.773 ± 0.016	3.158ProLys: 3.158 ± 0.022	3.069ProLeu: 3.069 ± 0.018	0.684ProMet: 0.684 ± 0.009	2.219ProAsn: 2.219 ± 0.016	1.652ProPro: 1.652 ± 0.032	1.481ProGln: 1.481 ± 0.013	1.422ProArg: 1.422 ± 0.013	3.098ProSer: 3.098 ± 0.022	1.685ProThr: 1.685 ± 0.014	1.858ProVal: 1.858 ± 0.012	0.345ProTrp: 0.345 ± 0.006	1.351ProTyr: 1.351 ± 0.011	0.002ProXaa: 0.002 ± 0.0
Gln	1.91GlnAla: 1.91 ± 0.012	0.629GlnCys: 0.629 ± 0.01	1.966GlnAsp: 1.966 ± 0.012	3.406GlnGlu: 3.406 ± 0.022	1.298GlnPhe: 1.298 ± 0.011	1.751GlnGly: 1.751 ± 0.014	0.597GlnHis: 0.597 ± 0.007	3.102GlnIle: 3.102 ± 0.016	3.635GlnLys: 3.635 ± 0.023	3.08GlnLeu: 3.08 ± 0.021	0.814GlnMet: 0.814 ± 0.009	2.627GlnAsn: 2.627 ± 0.017	0.987GlnPro: 0.987 ± 0.011	1.396GlnGln: 1.396 ± 0.013	1.509GlnArg: 1.509 ± 0.013	2.717GlnSer: 2.717 ± 0.014	1.777GlnThr: 1.777 ± 0.012	1.978GlnVal: 1.978 ± 0.014	0.291GlnTrp: 0.291 ± 0.005	1.201GlnTyr: 1.201 ± 0.01	0.003GlnXaa: 0.003 ± 0.0
Arg	1.802ArgAla: 1.802 ± 0.013	0.599ArgCys: 0.599 ± 0.008	1.965ArgAsp: 1.965 ± 0.015	2.983ArgGlu: 2.983 ± 0.021	1.774ArgPhe: 1.774 ± 0.013	1.688ArgGly: 1.688 ± 0.015	0.731ArgHis: 0.731 ± 0.009	3.404ArgIle: 3.404 ± 0.021	4.022ArgLys: 4.022 ± 0.026	3.57ArgLeu: 3.57 ± 0.02	0.934ArgMet: 0.934 ± 0.009	2.607ArgAsn: 2.607 ± 0.016	1.402ArgPro: 1.402 ± 0.013	1.392ArgGln: 1.392 ± 0.011	1.884ArgArg: 1.884 ± 0.016	2.925ArgSer: 2.925 ± 0.019	1.811ArgThr: 1.811 ± 0.015	2.15ArgVal: 2.15 ± 0.014	0.344ArgTrp: 0.344 ± 0.006	1.456ArgTyr: 1.456 ± 0.01	0.003ArgXaa: 0.003 ± 0.0
Ser	3.364SerAla: 3.364 ± 0.018	1.635SerCys: 1.635 ± 0.021	3.895SerAsp: 3.895 ± 0.019	5.87SerGlu: 5.87 ± 0.028	4.226SerPhe: 4.226 ± 0.02	3.745SerGly: 3.745 ± 0.027	1.519SerHis: 1.519 ± 0.01	6.824SerIle: 6.824 ± 0.029	6.989SerLys: 6.989 ± 0.027	8.755SerLeu: 8.755 ± 0.026	1.856SerMet: 1.856 ± 0.013	4.769SerAsn: 4.769 ± 0.02	3.399SerPro: 3.399 ± 0.024	3.409SerGln: 3.409 ± 0.016	3.252SerArg: 3.252 ± 0.02	7.92SerSer: 7.92 ± 0.048	3.972SerThr: 3.972 ± 0.025	3.891SerVal: 3.891 ± 0.018	0.691SerTrp: 0.691 ± 0.007	3.087SerTyr: 3.087 ± 0.019	0.006SerXaa: 0.006 ± 0.001
Thr	2.151ThrAla: 2.151 ± 0.016	1.055ThrCys: 1.055 ± 0.021	2.217ThrAsp: 2.217 ± 0.016	3.591ThrGlu: 3.591 ± 0.02	2.468ThrPhe: 2.468 ± 0.016	2.504ThrGly: 2.504 ± 0.02	0.92ThrHis: 0.92 ± 0.008	4.064ThrIle: 4.064 ± 0.023	3.933ThrLys: 3.933 ± 0.018	4.965ThrLeu: 4.965 ± 0.023	1.038ThrMet: 1.038 ± 0.01	2.711ThrAsn: 2.711 ± 0.016	2.224ThrPro: 2.224 ± 0.015	1.951ThrGln: 1.951 ± 0.014	1.846ThrArg: 1.846 ± 0.012	4.244ThrSer: 4.244 ± 0.026	2.455ThrThr: 2.455 ± 0.024	2.495ThrVal: 2.495 ± 0.018	0.485ThrTrp: 0.485 ± 0.007	1.843ThrTyr: 1.843 ± 0.015	0.004ThrXaa: 0.004 ± 0.001
Val	2.121ValAla: 2.121 ± 0.015	1.014ValCys: 1.014 ± 0.012	2.411ValAsp: 2.411 ± 0.017	3.32ValGlu: 3.32 ± 0.019	2.789ValPhe: 2.789 ± 0.017	2.079ValGly: 2.079 ± 0.015	0.922ValHis: 0.922 ± 0.009	3.925ValIle: 3.925 ± 0.021	4.428ValLys: 4.428 ± 0.02	4.93ValLeu: 4.93 ± 0.025	1.138ValMet: 1.138 ± 0.01	2.802ValAsn: 2.802 ± 0.015	1.717ValPro: 1.717 ± 0.011	1.707ValGln: 1.707 ± 0.012	1.933ValArg: 1.933 ± 0.014	3.937ValSer: 3.937 ± 0.023	2.296ValThr: 2.296 ± 0.017	2.755ValVal: 2.755 ± 0.019	0.592ValTrp: 0.592 ± 0.008	2.166ValTyr: 2.166 ± 0.014	0.004ValXaa: 0.004 ± 0.001
Trp	0.459TrpAla: 0.459 ± 0.007	0.161TrpCys: 0.161 ± 0.004	0.618TrpAsp: 0.618 ± 0.009	0.657TrpGlu: 0.657 ± 0.007	0.375TrpPhe: 0.375 ± 0.005	0.425TrpGly: 0.425 ± 0.007	0.168TrpHis: 0.168 ± 0.004	0.733TrpIle: 0.733 ± 0.007	0.923TrpLys: 0.923 ± 0.011	0.773TrpLeu: 0.773 ± 0.008	0.217TrpMet: 0.217 ± 0.004	0.694TrpAsn: 0.694 ± 0.01	0.266TrpPro: 0.266 ± 0.005	0.27TrpGln: 0.27 ± 0.004	0.378TrpArg: 0.378 ± 0.006	0.719TrpSer: 0.719 ± 0.008	0.459TrpThr: 0.459 ± 0.006	0.607TrpVal: 0.607 ± 0.006	0.087TrpTrp: 0.087 ± 0.003	0.301TrpTyr: 0.301 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.667TyrAla: 1.667 ± 0.012	0.905TyrCys: 0.905 ± 0.015	2.062TyrAsp: 2.062 ± 0.014	2.97TyrGlu: 2.97 ± 0.017	2.248TyrPhe: 2.248 ± 0.018	1.704TyrGly: 1.704 ± 0.013	0.702TyrHis: 0.702 ± 0.008	3.1TyrIle: 3.1 ± 0.017	3.338TyrLys: 3.338 ± 0.018	3.87TyrLeu: 3.87 ± 0.019	0.992TyrMet: 0.992 ± 0.008	2.435TyrAsn: 2.435 ± 0.018	1.274TyrPro: 1.274 ± 0.012	1.428TyrGln: 1.428 ± 0.011	1.527TyrArg: 1.527 ± 0.011	3.399TyrSer: 3.399 ± 0.019	2.098TyrThr: 2.098 ± 0.016	1.688TyrVal: 1.688 ± 0.013	0.414TyrTrp: 0.414 ± 0.006	1.914TyrTyr: 1.914 ± 0.02	0.003TyrXaa: 0.003 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.002XaaCys: 0.002 ± 0.0	0.003XaaAsp: 0.003 ± 0.0	0.004XaaGlu: 0.004 ± 0.001	0.004XaaPhe: 0.004 ± 0.001	0.003XaaGly: 0.003 ± 0.001	0.002XaaHis: 0.002 ± 0.0	0.009XaaIle: 0.009 ± 0.001	0.007XaaLys: 0.007 ± 0.001	0.009XaaLeu: 0.009 ± 0.001	0.004XaaMet: 0.004 ± 0.001	0.005XaaAsn: 0.005 ± 0.001	0.002XaaPro: 0.002 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.004XaaArg: 0.004 ± 0.001	0.006XaaSer: 0.006 ± 0.001	0.004XaaThr: 0.004 ± 0.001	0.004XaaVal: 0.004 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.003XaaTyr: 0.003 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 30969 proteins (13122840 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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