Corynebacterium glaucum
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2366 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q2HTL2|A0A1Q2HTL2_9CORY Integrase core domain protein OS=Corynebacterium glaucum OX=187491 GN=CGLAU_00960 PE=4 SV=1
MM1 pKa = 7.11 QKK3 pKa = 10.17 SAIWSVVAVAVVTVLVLAGAFVLLRR28 pKa = 11.84 GGEE31 pKa = 4.03 DD32 pKa = 3.6 DD33 pKa = 4.46 AASDD37 pKa = 3.88 AAPSEE42 pKa = 4.66 SAVGPSQITFVPNADD57 pKa = 3.24 QRR59 pKa = 11.84 ADD61 pKa = 3.64 CVAGGVGGIDD71 pKa = 3.85 LPCLGGEE78 pKa = 4.3 DD79 pKa = 4.36 VPGEE83 pKa = 4.16 LNDD86 pKa = 3.48 ITVVNLWAWWCEE98 pKa = 3.62 PCQRR102 pKa = 11.84 EE103 pKa = 4.7 LPITQALADD112 pKa = 3.78 AHH114 pKa = 6.59 PEE116 pKa = 3.86 YY117 pKa = 11.03 SVVGVHH123 pKa = 7.13 ADD125 pKa = 3.3 ANATNGVAMMNEE137 pKa = 4.23 LDD139 pKa = 3.81 VSFPSYY145 pKa = 11.02 QDD147 pKa = 3.04 DD148 pKa = 3.93 TNRR151 pKa = 11.84 FAGLQGLPGVVPITLVYY168 pKa = 10.38 QGGEE172 pKa = 4.03 PVGMFPQAFNSLEE185 pKa = 4.03 EE186 pKa = 4.35 LEE188 pKa = 4.47 AAVDD192 pKa = 4.05 GALSS196 pKa = 3.32
Molecular weight: 20.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.681
IPC_protein 3.656
Toseland 3.452
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.516
EMBOSS 3.592
Sillero 3.783
Patrickios 0.896
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|A0A1Q2HTU3|A0A1Q2HTU3_9CORY Uncharacterized protein OS=Corynebacterium glaucum OX=187491 GN=CGLAU_01335 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.04 HH17 pKa = 5.39 RR18 pKa = 11.84 KK19 pKa = 5.37 MLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2366
0
2366
760833
29
3023
321.6
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.091 ± 0.062
0.616 ± 0.013
6.161 ± 0.045
6.684 ± 0.057
3.346 ± 0.027
8.356 ± 0.043
2.035 ± 0.024
4.875 ± 0.04
2.947 ± 0.033
9.364 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.257 ± 0.022
2.851 ± 0.031
5.217 ± 0.042
3.356 ± 0.031
6.214 ± 0.046
5.513 ± 0.033
6.196 ± 0.042
8.272 ± 0.043
1.39 ± 0.024
2.262 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here