Pseudosporangium ferrugineum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Pseudosporangium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0RI42|A0A2T0RI42_9ACTN Dye decolorizing peroxidase OS=Pseudosporangium ferrugineum OX=439699 GN=CLV70_12236 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 5.63SSPTLQDD9 pKa = 3.27FVLNLIYY16 pKa = 10.8DD17 pKa = 4.28PAARR21 pKa = 11.84SAFEE25 pKa = 4.53LDD27 pKa = 3.62PEE29 pKa = 4.87GVLADD34 pKa = 4.38AGLSDD39 pKa = 3.78VTAVDD44 pKa = 3.73VQEE47 pKa = 4.5VIPLVVDD54 pKa = 3.42YY55 pKa = 11.36APLSDD60 pKa = 3.85VAAVTAPLGVHH71 pKa = 7.1DD72 pKa = 5.36LTTGVADD79 pKa = 4.28ADD81 pKa = 3.7VSAAVAQLQAMTGNLTPGAHH101 pKa = 7.02AAPDD105 pKa = 3.48VAYY108 pKa = 9.95AGAGAFMVSTDD119 pKa = 3.51GLLAGTPAVGVGLEE133 pKa = 4.06HH134 pKa = 7.27AATVDD139 pKa = 3.71APVSTSVTAPVDD151 pKa = 3.64GALSAVHH158 pKa = 7.15DD159 pKa = 4.88PGLGLDD165 pKa = 4.2AGVSTATSVTTTVVGDD181 pKa = 3.86ADD183 pKa = 3.89GLIASTGVDD192 pKa = 3.27AGATLDD198 pKa = 3.72GTASVVTGVTSTIGLHH214 pKa = 6.9DD215 pKa = 4.0VADD218 pKa = 4.56LDD220 pKa = 4.26VTHH223 pKa = 7.53LDD225 pKa = 3.63PAVSSVVGSVTGSDD239 pKa = 3.34PLGAVSHH246 pKa = 7.3DD247 pKa = 3.51ATQGVLDD254 pKa = 3.79VAGDD258 pKa = 3.56VTSPVTGLVDD268 pKa = 3.72GLGHH272 pKa = 6.01HH273 pKa = 6.73TAPDD277 pKa = 3.97TADD280 pKa = 3.53HH281 pKa = 6.29AHH283 pKa = 6.44GAVTDD288 pKa = 3.99LLFF291 pKa = 4.96

Molecular weight: 28.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0SA04|A0A2T0SA04_9ACTN Uncharacterized protein OS=Pseudosporangium ferrugineum OX=439699 GN=CLV70_105381 PE=4 SV=1
MM1 pKa = 7.72PSDD4 pKa = 3.85PTSPSDD10 pKa = 4.23PIRR13 pKa = 11.84STGPRR18 pKa = 11.84RR19 pKa = 11.84CTRR22 pKa = 11.84RR23 pKa = 11.84SGPNRR28 pKa = 11.84RR29 pKa = 11.84ALPRR33 pKa = 11.84SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GPCSGRR44 pKa = 11.84SRR46 pKa = 11.84CTRR49 pKa = 11.84PRR51 pKa = 11.84GPSRR55 pKa = 11.84RR56 pKa = 11.84APRR59 pKa = 11.84SGRR62 pKa = 11.84SPSTRR67 pKa = 11.84HH68 pKa = 6.21RR69 pKa = 11.84GRR71 pKa = 11.84SRR73 pKa = 11.84CSRR76 pKa = 11.84RR77 pKa = 11.84ARR79 pKa = 11.84TRR81 pKa = 11.84PPPRR85 pKa = 11.84SRR87 pKa = 11.84PSRR90 pKa = 11.84NSGTAPRR97 pKa = 11.84HH98 pKa = 4.83PPSRR102 pKa = 11.84RR103 pKa = 11.84PAARR107 pKa = 11.84WTTARR112 pKa = 11.84QRR114 pKa = 11.84SRR116 pKa = 11.84TTARR120 pKa = 11.84HH121 pKa = 5.46AGAPTARR128 pKa = 11.84NPRR131 pKa = 11.84PTPASRR137 pKa = 11.84SHH139 pKa = 5.16SARR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84SRR146 pKa = 11.84SPPARR151 pKa = 11.84SPPSRR156 pKa = 11.84RR157 pKa = 11.84PSLSPAIPSPRR168 pKa = 11.84GLSPPSRR175 pKa = 11.84RR176 pKa = 11.84PSLSPPNPSPPDD188 pKa = 3.35LSPPSRR194 pKa = 11.84RR195 pKa = 11.84VVRR198 pKa = 11.84SRR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84RR203 pKa = 11.84SWSRR207 pKa = 11.84SSRR210 pKa = 11.84RR211 pKa = 11.84LRR213 pKa = 11.84WSARR217 pKa = 11.84PRR219 pKa = 11.84PSPHH223 pKa = 6.64RR224 pKa = 11.84RR225 pKa = 11.84SCAPRR230 pKa = 11.84RR231 pKa = 11.84PSRR234 pKa = 11.84PRR236 pKa = 11.84RR237 pKa = 11.84PPARR241 pKa = 11.84RR242 pKa = 11.84RR243 pKa = 11.84PNRR246 pKa = 11.84PTSARR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84HH255 pKa = 5.27TPTSPSPGTPPPRR268 pKa = 11.84TTAGRR273 pKa = 11.84PLRR276 pKa = 11.84PRR278 pKa = 11.84QRR280 pKa = 11.84WHH282 pKa = 5.81QPLPRR287 pKa = 11.84RR288 pKa = 11.84PHH290 pKa = 6.01PPLWTLRR297 pKa = 11.84PRR299 pKa = 11.84PLRR302 pKa = 11.84RR303 pKa = 11.84HH304 pKa = 5.64RR305 pKa = 11.84RR306 pKa = 11.84PPRR309 pKa = 11.84RR310 pKa = 11.84PRR312 pKa = 11.84SPRR315 pKa = 11.84RR316 pKa = 11.84RR317 pKa = 11.84LHH319 pKa = 6.38RR320 pKa = 11.84RR321 pKa = 11.84RR322 pKa = 11.84LPLRR326 pKa = 11.84HH327 pKa = 5.03QHH329 pKa = 4.5QHH331 pKa = 4.08RR332 pKa = 11.84HH333 pKa = 4.28RR334 pKa = 11.84HH335 pKa = 4.17RR336 pKa = 11.84HH337 pKa = 4.49PLQRR341 pKa = 11.84QRR343 pKa = 11.84PLPRR347 pKa = 11.84RR348 pKa = 11.84CRR350 pKa = 11.84LPRR353 pKa = 11.84RR354 pKa = 11.84RR355 pKa = 11.84PRR357 pKa = 11.84PLPPPRR363 pKa = 11.84LRR365 pKa = 11.84PRR367 pKa = 11.84RR368 pKa = 11.84PPRR371 pKa = 11.84PLLPPRR377 pKa = 11.84LRR379 pKa = 11.84PRR381 pKa = 11.84RR382 pKa = 11.84PQRR385 pKa = 11.84PLPRR389 pKa = 11.84RR390 pKa = 11.84AQRR393 pKa = 11.84PLLPPRR399 pKa = 11.84LRR401 pKa = 11.84PRR403 pKa = 11.84RR404 pKa = 11.84PQRR407 pKa = 11.84PLPRR411 pKa = 11.84RR412 pKa = 11.84RR413 pKa = 11.84PRR415 pKa = 11.84LLPRR419 pKa = 11.84LRR421 pKa = 11.84HH422 pKa = 5.72RR423 pKa = 11.84PLPRR427 pKa = 11.84RR428 pKa = 11.84PPLPHH433 pKa = 6.92RR434 pKa = 11.84RR435 pKa = 11.84PLLRR439 pKa = 11.84RR440 pKa = 11.84RR441 pKa = 11.84CRR443 pKa = 11.84PHH445 pKa = 7.13RR446 pKa = 11.84LLLSHH451 pKa = 7.5RR452 pKa = 11.84SRR454 pKa = 11.84LSRR457 pKa = 11.84RR458 pKa = 11.84RR459 pKa = 11.84RR460 pKa = 11.84LLLSRR465 pKa = 11.84RR466 pKa = 11.84RR467 pKa = 11.84PACPPSRR474 pKa = 11.84RR475 pKa = 11.84KK476 pKa = 10.08RR477 pKa = 11.84PARR480 pKa = 11.84RR481 pKa = 11.84QRR483 pKa = 11.84LRR485 pKa = 11.84LRR487 pKa = 11.84LLRR490 pKa = 11.84FRR492 pKa = 4.94

Molecular weight: 58.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7729

0

7729

2588345

28

6957

334.9

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.352 ± 0.048

0.696 ± 0.008

6.0 ± 0.02

5.088 ± 0.024

2.688 ± 0.017

9.544 ± 0.027

2.092 ± 0.014

3.313 ± 0.019

1.772 ± 0.02

10.361 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.67 ± 0.01

1.784 ± 0.016

6.311 ± 0.03

2.585 ± 0.016

8.388 ± 0.033

4.861 ± 0.02

6.141 ± 0.024

8.77 ± 0.025

1.521 ± 0.011

2.063 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski