Desulforhopalus sp. IMCC35007

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulforhopalus; unclassified Desulforhopalus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4U0ZHK0|A0A4U0ZHK0_9DELT Glutamate--cysteine ligase OS=Desulforhopalus sp. IMCC35007 OX=2569543 GN=gshA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.31KK3 pKa = 9.88ILITCAALLCSCIATTVIAGAGSGPDD29 pKa = 3.54ADD31 pKa = 3.54WAGDD35 pKa = 3.64YY36 pKa = 10.81GFSSLTVTPIGTADD50 pKa = 4.31WINDD54 pKa = 3.62TAEE57 pKa = 4.19LQVGTTYY64 pKa = 11.06SVDD67 pKa = 3.29ATVLYY72 pKa = 10.84ADD74 pKa = 4.79DD75 pKa = 3.88VQGGGIYY82 pKa = 9.91PYY84 pKa = 11.27ASATTLEE91 pKa = 4.27LAIGSLSWASTADD104 pKa = 3.34ARR106 pKa = 11.84DD107 pKa = 3.63IEE109 pKa = 4.62PGGSGEE115 pKa = 4.49EE116 pKa = 4.08PDD118 pKa = 4.8FDD120 pKa = 3.71GASYY124 pKa = 11.19DD125 pKa = 3.46FSFSVDD131 pKa = 2.51VDD133 pKa = 3.31ASMLGEE139 pKa = 4.09TLASFSLSGLAAEE152 pKa = 4.77TGFDD156 pKa = 3.57INEE159 pKa = 4.21GTSFTKK165 pKa = 10.74DD166 pKa = 2.78MGSYY170 pKa = 10.07DD171 pKa = 3.51INVNEE176 pKa = 4.52PVPEE180 pKa = 4.48PSTILLFGAGLAGLVGFSRR199 pKa = 11.84RR200 pKa = 11.84KK201 pKa = 9.7RR202 pKa = 11.84KK203 pKa = 9.5AA204 pKa = 2.87

Molecular weight:
21.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4U0ZS27|A0A4U0ZS27_9DELT YwbE family protein OS=Desulforhopalus sp. IMCC35007 OX=2569543 GN=FCL48_10720 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSRR10 pKa = 11.84IKK12 pKa = 10.5RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.99GFRR20 pKa = 11.84EE21 pKa = 4.15RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AIIRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.8GRR40 pKa = 11.84KK41 pKa = 8.91RR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight:
5.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4843

0

4843

1630847

26

2943

336.7

37.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.936 ± 0.037

1.313 ± 0.017

5.34 ± 0.031

6.432 ± 0.03

4.377 ± 0.026

7.296 ± 0.033

2.076 ± 0.017

7.05 ± 0.029

5.803 ± 0.036

10.352 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.019

3.913 ± 0.022

4.075 ± 0.022

3.707 ± 0.021

4.804 ± 0.03

6.412 ± 0.028

5.545 ± 0.032

6.897 ± 0.029

1.047 ± 0.014

3.038 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski