Desulforhopalus sp. IMCC35007
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4843 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U0ZHK0|A0A4U0ZHK0_9DELT Glutamate--cysteine ligase OS=Desulforhopalus sp. IMCC35007 OX=2569543 GN=gshA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.31 KK3 pKa = 9.88 ILITCAALLCSCIATTVIAGAGSGPDD29 pKa = 3.54 ADD31 pKa = 3.54 WAGDD35 pKa = 3.64 YY36 pKa = 10.81 GFSSLTVTPIGTADD50 pKa = 4.31 WINDD54 pKa = 3.62 TAEE57 pKa = 4.19 LQVGTTYY64 pKa = 11.06 SVDD67 pKa = 3.29 ATVLYY72 pKa = 10.84 ADD74 pKa = 4.79 DD75 pKa = 3.88 VQGGGIYY82 pKa = 9.91 PYY84 pKa = 11.27 ASATTLEE91 pKa = 4.27 LAIGSLSWASTADD104 pKa = 3.34 ARR106 pKa = 11.84 DD107 pKa = 3.63 IEE109 pKa = 4.62 PGGSGEE115 pKa = 4.49 EE116 pKa = 4.08 PDD118 pKa = 4.8 FDD120 pKa = 3.71 GASYY124 pKa = 11.19 DD125 pKa = 3.46 FSFSVDD131 pKa = 2.51 VDD133 pKa = 3.31 ASMLGEE139 pKa = 4.09 TLASFSLSGLAAEE152 pKa = 4.77 TGFDD156 pKa = 3.57 INEE159 pKa = 4.21 GTSFTKK165 pKa = 10.74 DD166 pKa = 2.78 MGSYY170 pKa = 10.07 DD171 pKa = 3.51 INVNEE176 pKa = 4.52 PVPEE180 pKa = 4.48 PSTILLFGAGLAGLVGFSRR199 pKa = 11.84 RR200 pKa = 11.84 KK201 pKa = 9.7 RR202 pKa = 11.84 KK203 pKa = 9.5 AA204 pKa = 2.87
Molecular weight: 21.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.414
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|A0A4U0ZS27|A0A4U0ZS27_9DELT YwbE family protein OS=Desulforhopalus sp. IMCC35007 OX=2569543 GN=FCL48_10720 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSRR10 pKa = 11.84 IKK12 pKa = 10.5 RR13 pKa = 11.84 ARR15 pKa = 11.84 THH17 pKa = 5.99 GFRR20 pKa = 11.84 EE21 pKa = 4.15 RR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIIRR33 pKa = 11.84 ARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.8 GRR40 pKa = 11.84 KK41 pKa = 8.91 RR42 pKa = 11.84 LSAA45 pKa = 3.96
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.369
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4843
0
4843
1630847
26
2943
336.7
37.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.936 ± 0.037
1.313 ± 0.017
5.34 ± 0.031
6.432 ± 0.03
4.377 ± 0.026
7.296 ± 0.033
2.076 ± 0.017
7.05 ± 0.029
5.803 ± 0.036
10.352 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.59 ± 0.019
3.913 ± 0.022
4.075 ± 0.022
3.707 ± 0.021
4.804 ± 0.03
6.412 ± 0.028
5.545 ± 0.032
6.897 ± 0.029
1.047 ± 0.014
3.038 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here