Oceanispirochaeta crateris

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Spirochaetaceae; Oceanispirochaeta

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3504 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1QMT4|A0A5C1QMT4_9SPIO FadR family transcriptional regulator OS=Oceanispirochaeta crateris OX=2518645 GN=EXM22_09825 PE=4 SV=1
MM1 pKa = 7.44NLFKK5 pKa = 10.82SAVIAAVLGVSITVIGCQTNGKK27 pKa = 8.91EE28 pKa = 4.15LTQEE32 pKa = 3.92SPKK35 pKa = 10.78VKK37 pKa = 10.22FVVTDD42 pKa = 3.51AMQTKK47 pKa = 10.17FYY49 pKa = 8.24DD50 pKa = 3.28THH52 pKa = 8.11GNIIEE57 pKa = 4.28EE58 pKa = 4.6PEE60 pKa = 4.69PGDD63 pKa = 3.56PLYY66 pKa = 11.2GQDD69 pKa = 3.52AQYY72 pKa = 11.28EE73 pKa = 4.92GIRR76 pKa = 11.84PLYY79 pKa = 8.99TDD81 pKa = 4.04NGDD84 pKa = 3.66GTVTDD89 pKa = 5.08DD90 pKa = 3.65NTGLMWQQTPPKK102 pKa = 10.77DD103 pKa = 3.13KK104 pKa = 8.68MTYY107 pKa = 9.98DD108 pKa = 3.16EE109 pKa = 4.23AMEE112 pKa = 4.01YY113 pKa = 10.57VEE115 pKa = 4.85KK116 pKa = 11.22LEE118 pKa = 4.83LGGYY122 pKa = 7.02TDD124 pKa = 3.46WRR126 pKa = 11.84LPTIKK131 pKa = 10.34EE132 pKa = 4.27SFSLANLDD140 pKa = 3.48GQLNVGDD147 pKa = 4.41TNQSVPYY154 pKa = 9.66IDD156 pKa = 4.77SDD158 pKa = 4.18YY159 pKa = 11.43FDD161 pKa = 4.35FFYY164 pKa = 11.19DD165 pKa = 3.01EE166 pKa = 4.34GRR168 pKa = 11.84PYY170 pKa = 9.98TGSYY174 pKa = 5.6WTSSVTRR181 pKa = 11.84IPAEE185 pKa = 3.8NEE187 pKa = 3.72YY188 pKa = 11.34EE189 pKa = 4.16EE190 pKa = 4.35MEE192 pKa = 4.36KK193 pKa = 10.83NYY195 pKa = 10.32GFNWADD201 pKa = 3.35GHH203 pKa = 6.23LKK205 pKa = 10.58SYY207 pKa = 11.16GDD209 pKa = 4.23GYY211 pKa = 11.34SLDD214 pKa = 3.84GSTSGSSIPAGVRR227 pKa = 11.84AVRR230 pKa = 11.84GEE232 pKa = 3.9EE233 pKa = 3.9GVYY236 pKa = 10.46GVNDD240 pKa = 3.48FTDD243 pKa = 3.47NRR245 pKa = 11.84DD246 pKa = 3.29GTITDD251 pKa = 3.46QATGLMWSQKK261 pKa = 10.86DD262 pKa = 3.48SGAVNDD268 pKa = 4.75DD269 pKa = 3.34GTLRR273 pKa = 11.84SEE275 pKa = 4.16DD276 pKa = 4.1DD277 pKa = 3.6EE278 pKa = 4.89NFGLGRR284 pKa = 11.84TWVDD288 pKa = 3.08TLVWVQNMNAAQYY301 pKa = 10.73LGYY304 pKa = 10.16SDD306 pKa = 4.31WRR308 pKa = 11.84LPDD311 pKa = 3.68AKK313 pKa = 9.69EE314 pKa = 3.83LQSIVQYY321 pKa = 10.82GISEE325 pKa = 4.31LPATDD330 pKa = 3.8SDD332 pKa = 4.44YY333 pKa = 11.58FDD335 pKa = 5.05LSRR338 pKa = 11.84TDD340 pKa = 3.08SYY342 pKa = 10.88MWTSTTCGDD351 pKa = 4.09FPDD354 pKa = 3.84TALYY358 pKa = 9.95FAFGKK363 pKa = 10.6AYY365 pKa = 10.12GINLMTGGVPAGQGPEE381 pKa = 4.19GGEE384 pKa = 3.97QMGPPPEE391 pKa = 4.74DD392 pKa = 3.33VNDD395 pKa = 3.7PMEE398 pKa = 4.7NEE400 pKa = 4.07VEE402 pKa = 4.38SFVTVDD408 pKa = 2.97ATVADD413 pKa = 4.82FVDD416 pKa = 3.73THH418 pKa = 6.92GPGAMRR424 pKa = 11.84NDD426 pKa = 3.76YY427 pKa = 11.23KK428 pKa = 11.2DD429 pKa = 3.42VTGTATQAPALSRR442 pKa = 11.84EE443 pKa = 4.39LWSKK447 pKa = 11.12LYY449 pKa = 10.94GEE451 pKa = 5.07EE452 pKa = 4.12YY453 pKa = 9.94PYY455 pKa = 11.44DD456 pKa = 4.34FDD458 pKa = 5.98PSDD461 pKa = 3.51EE462 pKa = 4.25TTLFDD467 pKa = 5.62LSASEE472 pKa = 4.16NAADD476 pKa = 4.82YY477 pKa = 10.82IVLYY481 pKa = 10.7NYY483 pKa = 10.99ALLVRR488 pKa = 11.84DD489 pKa = 3.99ADD491 pKa = 3.7

Molecular weight: 54.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1QLR6|A0A5C1QLR6_9SPIO PAS domain S-box protein OS=Oceanispirochaeta crateris OX=2518645 GN=EXM22_10740 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.23KK4 pKa = 10.87SMIVKK9 pKa = 10.1CNRR12 pKa = 11.84KK13 pKa = 8.78QKK15 pKa = 10.62FSTRR19 pKa = 11.84EE20 pKa = 3.64YY21 pKa = 10.2NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PRR31 pKa = 11.84GYY33 pKa = 8.93MRR35 pKa = 11.84KK36 pKa = 9.48FEE38 pKa = 3.99MCRR41 pKa = 11.84ICFRR45 pKa = 11.84KK46 pKa = 9.58LANEE50 pKa = 3.78GMIPGVTKK58 pKa = 10.78SSWW61 pKa = 2.87

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3504

0

3504

1208583

19

3847

344.9

38.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.43 ± 0.042

0.979 ± 0.016

5.644 ± 0.037

7.063 ± 0.046

4.677 ± 0.029

7.025 ± 0.041

1.834 ± 0.02

7.692 ± 0.043

6.142 ± 0.043

10.727 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.018

4.276 ± 0.03

3.905 ± 0.024

3.336 ± 0.023

4.641 ± 0.036

7.423 ± 0.035

4.925 ± 0.041

5.99 ± 0.035

1.052 ± 0.013

3.411 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski