Zebra finch circovirus
Average proteome isoelectric point is 8.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142LXX1|A0A142LXX1_9CIRC Capsid protein OS=Zebra finch circovirus OX=1642515 GN=cap PE=3 SV=1
MM1 pKa = 7.84 PKK3 pKa = 9.54 QARR6 pKa = 11.84 EE7 pKa = 4.38 SPCKK11 pKa = 10.03 RR12 pKa = 11.84 WVFTLNNPTEE22 pKa = 4.21 EE23 pKa = 3.96 EE24 pKa = 4.25 VEE26 pKa = 4.26 NVKK29 pKa = 10.49 KK30 pKa = 10.74 LPPSEE35 pKa = 3.77 YY36 pKa = 9.92 HH37 pKa = 5.22 YY38 pKa = 11.01 AIVGKK43 pKa = 10.28 EE44 pKa = 3.88 KK45 pKa = 11.02 GEE47 pKa = 3.86 QGTPHH52 pKa = 6.35 LQGFLHH58 pKa = 6.63 LKK60 pKa = 10.07 KK61 pKa = 10.27 KK62 pKa = 10.34 VRR64 pKa = 11.84 LNQMKK69 pKa = 10.0 QLLPRR74 pKa = 11.84 AHH76 pKa = 6.96 FEE78 pKa = 3.81 RR79 pKa = 11.84 ARR81 pKa = 11.84 GSDD84 pKa = 3.51 EE85 pKa = 6.24 DD86 pKa = 3.95 NEE88 pKa = 4.6 QYY90 pKa = 10.46 CSKK93 pKa = 10.32 EE94 pKa = 3.49 GDD96 pKa = 3.67 VILTIGAPAAGNRR109 pKa = 11.84 SDD111 pKa = 4.56 LAGAVAAVKK120 pKa = 10.28 AGRR123 pKa = 11.84 AMTEE127 pKa = 3.85 VARR130 pKa = 11.84 EE131 pKa = 3.7 FSEE134 pKa = 6.33 AYY136 pKa = 9.91 VKK138 pKa = 10.05 WGRR141 pKa = 11.84 GLKK144 pKa = 10.32 DD145 pKa = 3.42 LALMIGQKK153 pKa = 10.19 PRR155 pKa = 11.84 DD156 pKa = 3.87 FKK158 pKa = 11.24 TEE160 pKa = 4.14 VIVLTGPSGVGKK172 pKa = 10.26 SRR174 pKa = 11.84 WANEE178 pKa = 3.68 QEE180 pKa = 4.04 GTKK183 pKa = 9.85 FYY185 pKa = 11.31 KK186 pKa = 10.05 MKK188 pKa = 10.64 GDD190 pKa = 2.93 WWDD193 pKa = 3.88 GYY195 pKa = 10.06 SNEE198 pKa = 5.05 DD199 pKa = 3.24 IVVIDD204 pKa = 5.7 DD205 pKa = 4.34 FYY207 pKa = 11.69 GWIPFCEE214 pKa = 4.32 LLRR217 pKa = 11.84 LTDD220 pKa = 4.49 RR221 pKa = 11.84 YY222 pKa = 8.43 PHH224 pKa = 6.25 KK225 pKa = 11.17 VPVKK229 pKa = 8.85 GSYY232 pKa = 10.43 VEE234 pKa = 4.26 FTSKK238 pKa = 10.77 KK239 pKa = 10.29 IIITSNTHH247 pKa = 5.69 PDD249 pKa = 2.53 HH250 pKa = 6.87 WYY252 pKa = 10.6 SEE254 pKa = 4.43 EE255 pKa = 3.85 KK256 pKa = 10.68 CYY258 pKa = 10.95 VQALFRR264 pKa = 11.84 RR265 pKa = 11.84 INKK268 pKa = 8.45 WLMWDD273 pKa = 3.24 VFGFVDD279 pKa = 5.43 APDD282 pKa = 3.62 AVKK285 pKa = 10.33 KK286 pKa = 10.86 YY287 pKa = 9.37 PINYY291 pKa = 9.36
Molecular weight: 33.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.902
IPC2_protein 8.039
IPC_protein 7.951
Toseland 8.346
ProMoST 8.565
Dawson 8.799
Bjellqvist 8.799
Wikipedia 8.858
Rodwell 8.916
Grimsley 8.39
Solomon 8.931
Lehninger 8.916
Nozaki 8.931
DTASelect 8.668
Thurlkill 8.77
EMBOSS 8.975
Sillero 8.99
Patrickios 4.571
IPC_peptide 8.931
IPC2_peptide 7.497
IPC2.peptide.svr19 7.626
Protein with the highest isoelectric point:
>tr|A0A142LXX3|A0A142LXX3_9CIRC ORFC4 OS=Zebra finch circovirus OX=1642515 GN=ORFC4 PE=4 SV=1
MM1 pKa = 7.76 NEE3 pKa = 3.92 YY4 pKa = 10.79 CPLRR8 pKa = 11.84 TTAFAWPKK16 pKa = 10.18 SRR18 pKa = 11.84 PSPAMRR24 pKa = 11.84 TGRR27 pKa = 11.84 TGRR30 pKa = 11.84 GRR32 pKa = 11.84 ATPSPSKK39 pKa = 8.43 THH41 pKa = 4.61 TWEE44 pKa = 4.2 TSSKK48 pKa = 10.29 RR49 pKa = 11.84 PSCRR53 pKa = 11.84 PTRR56 pKa = 11.84 WPIGTEE62 pKa = 4.12 PEE64 pKa = 4.13 SGTCAKK70 pKa = 10.57 ASNAYY75 pKa = 10.03 SGPDD79 pKa = 3.26 HH80 pKa = 7.0 SCPP83 pKa = 3.98
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.311
IPC_protein 9.604
Toseland 10.16
ProMoST 9.853
Dawson 10.321
Bjellqvist 10.058
Wikipedia 10.496
Rodwell 10.613
Grimsley 10.379
Solomon 10.394
Lehninger 10.379
Nozaki 10.292
DTASelect 10.014
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.277
Patrickios 10.423
IPC_peptide 10.394
IPC2_peptide 9.414
IPC2.peptide.svr19 8.193
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
680
62
291
170.0
19.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.059 ± 0.745
1.324 ± 0.811
4.265 ± 0.898
5.882 ± 1.061
3.824 ± 0.727
7.5 ± 1.636
2.941 ± 0.355
3.529 ± 0.676
6.324 ± 1.741
7.059 ± 1.223
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.206 ± 0.126
3.824 ± 0.375
7.059 ± 1.564
3.235 ± 0.755
10.294 ± 2.659
5.0 ± 1.745
6.912 ± 1.782
4.853 ± 1.609
3.529 ± 0.381
3.382 ± 0.65
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here