Maize-associated totivirus 1
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9MPY0|A0A0N9MPY0_9VIRU Capsid protein OS=Maize-associated totivirus 1 OX=1729670 PE=4 SV=1
MM1 pKa = 7.59 DD2 pKa = 3.86 TFVRR6 pKa = 11.84 HH7 pKa = 5.63 FFKK10 pKa = 10.78 EE11 pKa = 4.03 VTTPGVPFVLDD22 pKa = 4.21 DD23 pKa = 4.33 ACHH26 pKa = 5.44 TWTYY30 pKa = 10.17 FQGFKK35 pKa = 10.36 TVVTGDD41 pKa = 3.39 ASKK44 pKa = 11.15 SSARR48 pKa = 11.84 DD49 pKa = 3.04 IYY51 pKa = 11.26 QAMTPLGMLEE61 pKa = 4.1 TAFEE65 pKa = 4.71 SMGSNYY71 pKa = 10.7 DD72 pKa = 3.6 GLTKK76 pKa = 10.65 NYY78 pKa = 7.33 LTPEE82 pKa = 3.93 GAINYY87 pKa = 9.99 SNIHH91 pKa = 6.41 DD92 pKa = 4.03 TLKK95 pKa = 10.89 QSGGMNNQEE104 pKa = 4.83 LSTFMAFCQTISWHH118 pKa = 6.73 DD119 pKa = 3.44 NHH121 pKa = 5.78 VTLLFNMLRR130 pKa = 11.84 YY131 pKa = 9.76 VILMKK136 pKa = 10.75 SKK138 pKa = 10.5 EE139 pKa = 4.34 SKK141 pKa = 10.33 QNLNGALGRR150 pKa = 11.84 FQEE153 pKa = 5.08 DD154 pKa = 3.44 FLEE157 pKa = 5.11 INMDD161 pKa = 4.33 PACQLPAGFEE171 pKa = 3.71 LALRR175 pKa = 11.84 DD176 pKa = 3.36 RR177 pKa = 11.84 VAATRR182 pKa = 11.84 PRR184 pKa = 11.84 ARR186 pKa = 11.84 QAARR190 pKa = 11.84 VTVACPLLPFPGVPTLLLPTQCTPYY215 pKa = 10.35 PIPFEE220 pKa = 4.04 LAGKK224 pKa = 10.2 FDD226 pKa = 3.9 DD227 pKa = 3.74 TMGRR231 pKa = 11.84 FDD233 pKa = 4.85 RR234 pKa = 11.84 RR235 pKa = 11.84 GTRR238 pKa = 11.84 LKK240 pKa = 10.25 IDD242 pKa = 3.56 VAWRR246 pKa = 11.84 VMNFLQDD253 pKa = 4.1 QSYY256 pKa = 8.05 TWQCEE261 pKa = 3.87 YY262 pKa = 10.67 RR263 pKa = 11.84 GTRR266 pKa = 11.84 IGQQEE271 pKa = 4.44 YY272 pKa = 10.05 VRR274 pKa = 11.84 GRR276 pKa = 11.84 YY277 pKa = 9.93 SNMIWPVLYY286 pKa = 10.44 QPEE289 pKa = 4.66 DD290 pKa = 3.51 NDD292 pKa = 3.26 EE293 pKa = 4.28 DD294 pKa = 4.11 VVLRR298 pKa = 11.84 RR299 pKa = 11.84 ITEE302 pKa = 3.81 VGLPDD307 pKa = 3.66 VPLPPMHH314 pKa = 7.07 NEE316 pKa = 3.66 FFKK319 pKa = 11.18 GKK321 pKa = 9.26 SVEE324 pKa = 4.05 YY325 pKa = 9.53 KK326 pKa = 8.65 YY327 pKa = 11.15 QSIRR331 pKa = 11.84 RR332 pKa = 11.84 GLAARR337 pKa = 11.84 FRR339 pKa = 11.84 GAKK342 pKa = 9.25 IDD344 pKa = 3.37 ISSYY348 pKa = 8.68 GAHH351 pKa = 5.7 VPVLKK356 pKa = 10.57 KK357 pKa = 10.46 ISTVTYY363 pKa = 10.22 AVNEE367 pKa = 4.09 GLRR370 pKa = 11.84 TVRR373 pKa = 11.84 GFIEE377 pKa = 4.44 RR378 pKa = 11.84 AAQDD382 pKa = 3.36 FRR384 pKa = 11.84 LAPVAGPGVIPPAEE398 pKa = 3.96 QAPDD402 pKa = 3.21 ASAATGGPEE411 pKa = 4.81 AGGTSAA417 pKa = 5.6
Molecular weight: 46.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.706
IPC2_protein 6.868
IPC_protein 6.971
Toseland 6.81
ProMoST 7.439
Dawson 7.571
Bjellqvist 7.614
Wikipedia 7.497
Rodwell 7.571
Grimsley 6.883
Solomon 7.644
Lehninger 7.658
Nozaki 7.878
DTASelect 7.732
Thurlkill 7.775
EMBOSS 7.834
Sillero 8.009
Patrickios 4.253
IPC_peptide 7.644
IPC2_peptide 7.22
IPC2.peptide.svr19 7.191
Protein with the highest isoelectric point:
>tr|A0A0N9MPY0|A0A0N9MPY0_9VIRU Capsid protein OS=Maize-associated totivirus 1 OX=1729670 PE=4 SV=1
MM1 pKa = 7.59 DD2 pKa = 3.86 TFVRR6 pKa = 11.84 HH7 pKa = 5.63 FFKK10 pKa = 10.78 EE11 pKa = 4.03 VTTPGVPFVLDD22 pKa = 4.21 DD23 pKa = 4.33 ACHH26 pKa = 5.44 TWTYY30 pKa = 10.17 FQGFKK35 pKa = 10.36 TVVTGDD41 pKa = 3.39 ASKK44 pKa = 11.15 SSARR48 pKa = 11.84 DD49 pKa = 3.04 IYY51 pKa = 11.26 QAMTPLGMLEE61 pKa = 4.1 TAFEE65 pKa = 4.71 SMGSNYY71 pKa = 10.7 DD72 pKa = 3.6 GLTKK76 pKa = 10.65 NYY78 pKa = 7.33 LTPEE82 pKa = 3.93 GAINYY87 pKa = 9.99 SNIHH91 pKa = 6.41 DD92 pKa = 4.03 TLKK95 pKa = 10.89 QSGGMNNQEE104 pKa = 4.83 LSTFMAFCQTISWHH118 pKa = 6.73 DD119 pKa = 3.44 NHH121 pKa = 5.78 VTLLFNMLRR130 pKa = 11.84 YY131 pKa = 9.76 VILMKK136 pKa = 10.75 SKK138 pKa = 10.5 EE139 pKa = 4.34 SKK141 pKa = 10.33 QNLNGALGRR150 pKa = 11.84 FQEE153 pKa = 5.08 DD154 pKa = 3.44 FLEE157 pKa = 5.11 INMDD161 pKa = 4.33 PACQLPAGFEE171 pKa = 3.71 LALRR175 pKa = 11.84 DD176 pKa = 3.36 RR177 pKa = 11.84 VAATRR182 pKa = 11.84 PRR184 pKa = 11.84 ARR186 pKa = 11.84 QAARR190 pKa = 11.84 VTVACPLLPFPGVPTLLLPTQCTPYY215 pKa = 10.35 PIPFEE220 pKa = 4.04 LAGKK224 pKa = 10.2 FDD226 pKa = 3.9 DD227 pKa = 3.74 TMGRR231 pKa = 11.84 FDD233 pKa = 4.85 RR234 pKa = 11.84 RR235 pKa = 11.84 GTRR238 pKa = 11.84 LKK240 pKa = 10.25 IDD242 pKa = 3.56 VAWRR246 pKa = 11.84 VMNFLQDD253 pKa = 4.1 QSYY256 pKa = 8.05 TWQCEE261 pKa = 3.87 YY262 pKa = 10.67 RR263 pKa = 11.84 GTRR266 pKa = 11.84 IGQQEE271 pKa = 4.44 YY272 pKa = 10.05 VRR274 pKa = 11.84 GRR276 pKa = 11.84 YY277 pKa = 9.93 SNMIWPVLYY286 pKa = 10.44 QPEE289 pKa = 4.66 DD290 pKa = 3.51 NDD292 pKa = 3.26 EE293 pKa = 4.28 DD294 pKa = 4.11 VVLRR298 pKa = 11.84 RR299 pKa = 11.84 ITEE302 pKa = 3.81 VGLPDD307 pKa = 3.66 VPLPPMHH314 pKa = 7.07 NEE316 pKa = 3.66 FFKK319 pKa = 11.18 GKK321 pKa = 9.26 SVEE324 pKa = 4.05 YY325 pKa = 9.53 KK326 pKa = 8.65 YY327 pKa = 11.15 QSIRR331 pKa = 11.84 RR332 pKa = 11.84 GLAARR337 pKa = 11.84 FRR339 pKa = 11.84 GAKK342 pKa = 9.25 IDD344 pKa = 3.37 ISSYY348 pKa = 8.68 GAHH351 pKa = 5.7 VPVLKK356 pKa = 10.57 KK357 pKa = 10.46 ISTVTYY363 pKa = 10.22 AVNEE367 pKa = 4.09 GLRR370 pKa = 11.84 TVRR373 pKa = 11.84 GFIEE377 pKa = 4.37 RR378 pKa = 11.84 AAQDD382 pKa = 3.76 FSSCPGCRR390 pKa = 11.84 SWRR393 pKa = 11.84 DD394 pKa = 3.19 TASRR398 pKa = 11.84 AGTRR402 pKa = 11.84 CLSCNRR408 pKa = 11.84 GTGSWWDD415 pKa = 3.31 QCLGEE420 pKa = 4.32 VAGEE424 pKa = 4.49 AITMLNATPGGDD436 pKa = 3.14 GPYY439 pKa = 9.82 IRR441 pKa = 11.84 RR442 pKa = 11.84 KK443 pKa = 10.2 EE444 pKa = 4.06 PLTLDD449 pKa = 3.61 NVPVWVNIRR458 pKa = 11.84 RR459 pKa = 11.84 NHH461 pKa = 4.9 LTAVDD466 pKa = 3.74 RR467 pKa = 11.84 QDD469 pKa = 3.35 AKK471 pKa = 10.89 HH472 pKa = 5.88 VLGFVLPGKK481 pKa = 9.84 SFEE484 pKa = 4.13 VGGFGRR490 pKa = 11.84 YY491 pKa = 8.27 LVGKK495 pKa = 8.18 TSVAATAIPCDD506 pKa = 4.6 GVWLMYY512 pKa = 10.81 VSVTTPANILPPIVKK527 pKa = 8.62 RR528 pKa = 11.84 TLSAMFSAVDD538 pKa = 4.04 GYY540 pKa = 11.2 YY541 pKa = 10.44 FHH543 pKa = 7.96 DD544 pKa = 4.89 ADD546 pKa = 3.74 TTKK549 pKa = 10.91 YY550 pKa = 10.19 LRR552 pKa = 11.84 KK553 pKa = 8.39 TFDD556 pKa = 2.72 VDD558 pKa = 3.81 RR559 pKa = 11.84 NVIAHH564 pKa = 7.69 AARR567 pKa = 11.84 PTPPHH572 pKa = 5.93 EE573 pKa = 4.74 GEE575 pKa = 4.1 FEE577 pKa = 4.09 RR578 pKa = 11.84 AVITAEE584 pKa = 3.76 HH585 pKa = 6.0 HH586 pKa = 3.99 THH588 pKa = 5.67 YY589 pKa = 10.47 RR590 pKa = 11.84 PEE592 pKa = 4.46 EE593 pKa = 3.34 IWAIAKK599 pKa = 8.59 QHH601 pKa = 4.88 TTTLRR606 pKa = 11.84 AMGIVLANLRR616 pKa = 11.84 KK617 pKa = 9.22 IQGVTEE623 pKa = 4.01 ATVATFLAYY632 pKa = 9.84 IMTVRR637 pKa = 11.84 PQVAYY642 pKa = 10.35 LVATSRR648 pKa = 11.84 RR649 pKa = 11.84 IWRR652 pKa = 11.84 SKK654 pKa = 10.3 NVAEE658 pKa = 4.08 LTEE661 pKa = 4.04 VLKK664 pKa = 11.12 DD665 pKa = 2.83 IATPIKK671 pKa = 10.62 SMHH674 pKa = 4.66 QHH676 pKa = 5.65 EE677 pKa = 4.78 VCDD680 pKa = 3.76 LTEE683 pKa = 4.52 LFEE686 pKa = 4.38 LQCLVNRR693 pKa = 11.84 GVGQIDD699 pKa = 3.11 WRR701 pKa = 11.84 KK702 pKa = 8.72 EE703 pKa = 3.84 RR704 pKa = 11.84 NHH706 pKa = 5.84 RR707 pKa = 11.84 TSPDD711 pKa = 3.32 VVKK714 pKa = 11.08 VSLEE718 pKa = 4.07 DD719 pKa = 3.41 VVKK722 pKa = 10.37 YY723 pKa = 9.31 ATEE726 pKa = 3.76 IFLLGKK732 pKa = 9.98 AHH734 pKa = 7.11 GYY736 pKa = 10.03 HH737 pKa = 5.4 YY738 pKa = 11.05 RR739 pKa = 11.84 KK740 pKa = 8.92 MDD742 pKa = 3.37 RR743 pKa = 11.84 QKK745 pKa = 10.91 YY746 pKa = 9.47 ISARR750 pKa = 11.84 WEE752 pKa = 3.68 WSPTGSIHH760 pKa = 5.86 SQYY763 pKa = 10.79 PEE765 pKa = 3.98 DD766 pKa = 3.77 EE767 pKa = 4.36 RR768 pKa = 11.84 YY769 pKa = 9.93 IPGNYY774 pKa = 8.18 RR775 pKa = 11.84 QKK777 pKa = 10.02 TKK779 pKa = 10.49 FVALNMMSRR788 pKa = 11.84 QHH790 pKa = 6.35 VDD792 pKa = 2.47 SMFLRR797 pKa = 11.84 KK798 pKa = 9.52 PEE800 pKa = 3.62 IHH802 pKa = 6.53 AWTSVKK808 pKa = 10.73 YY809 pKa = 9.62 EE810 pKa = 3.87 WAKK813 pKa = 10.25 QRR815 pKa = 11.84 AIYY818 pKa = 10.09 GVDD821 pKa = 3.3 LTSTVITNFAMYY833 pKa = 10.0 RR834 pKa = 11.84 CEE836 pKa = 4.02 EE837 pKa = 4.23 VFKK840 pKa = 11.11 HH841 pKa = 5.86 RR842 pKa = 11.84 FPVGEE847 pKa = 3.83 EE848 pKa = 3.42 AAADD852 pKa = 3.84 RR853 pKa = 11.84 VHH855 pKa = 6.86 RR856 pKa = 11.84 RR857 pKa = 11.84 LKK859 pKa = 10.67 IMLEE863 pKa = 4.24 DD864 pKa = 4.03 NEE866 pKa = 4.66 SFCYY870 pKa = 10.81 DD871 pKa = 3.25 FDD873 pKa = 5.14 DD874 pKa = 5.86 FNAQHH879 pKa = 6.09 STQAMQAVLVAYY891 pKa = 10.27 LNVFQADD898 pKa = 3.36 MSDD901 pKa = 3.81 DD902 pKa = 3.53 QRR904 pKa = 11.84 EE905 pKa = 4.13 AMSWVIDD912 pKa = 3.76 SLSDD916 pKa = 3.6 VTIHH920 pKa = 6.46 NSNVQPPEE928 pKa = 3.75 QYY930 pKa = 9.89 EE931 pKa = 4.25 LKK933 pKa = 9.94 GTLLSGWRR941 pKa = 11.84 LTTFMNTALNYY952 pKa = 10.15 VYY954 pKa = 10.1 FKK956 pKa = 11.06 AAGCFDD962 pKa = 3.23 IGGVRR967 pKa = 11.84 DD968 pKa = 3.92 SVHH971 pKa = 6.66 NGDD974 pKa = 5.02 DD975 pKa = 3.41 VLVSIKK981 pKa = 10.63 HH982 pKa = 5.58 IGAAVKK988 pKa = 9.6 IHH990 pKa = 6.28 HH991 pKa = 6.89 RR992 pKa = 11.84 MAQINARR999 pKa = 11.84 AQPAKK1004 pKa = 10.67 CNVFSVGEE1012 pKa = 3.91 FLRR1015 pKa = 11.84 VEE1017 pKa = 4.23 HH1018 pKa = 7.01 KK1019 pKa = 10.46 VDD1021 pKa = 3.32 KK1022 pKa = 10.22 DD1023 pKa = 3.38 TGLGAQYY1030 pKa = 9.02 LTRR1033 pKa = 11.84 ACATLVHH1040 pKa = 6.2 SRR1042 pKa = 11.84 CEE1044 pKa = 4.19 SQEE1047 pKa = 4.0 PEE1049 pKa = 4.31 HH1050 pKa = 6.18 LTGAVKK1056 pKa = 10.65 AIVTRR1061 pKa = 11.84 ARR1063 pKa = 11.84 EE1064 pKa = 3.86 VFEE1067 pKa = 4.02 RR1068 pKa = 11.84 AKK1070 pKa = 10.44 ISQALLADD1078 pKa = 4.33 LVRR1081 pKa = 11.84 SAIRR1085 pKa = 11.84 RR1086 pKa = 11.84 VAAVFHH1092 pKa = 6.74 RR1093 pKa = 11.84 PAKK1096 pKa = 8.7 EE1097 pKa = 3.65 AFVIAEE1103 pKa = 3.91 LHH1105 pKa = 5.85 AVVGGASTEE1114 pKa = 4.05 DD1115 pKa = 3.59 FAPIDD1120 pKa = 3.6 FKK1122 pKa = 11.3 INEE1125 pKa = 3.92 RR1126 pKa = 11.84 CEE1128 pKa = 3.96 YY1129 pKa = 10.67 DD1130 pKa = 3.55 RR1131 pKa = 11.84 EE1132 pKa = 4.17 RR1133 pKa = 11.84 CASDD1137 pKa = 3.45 KK1138 pKa = 11.45 DD1139 pKa = 4.28 DD1140 pKa = 3.67 MVVTQDD1146 pKa = 4.31 LYY1148 pKa = 10.84 PGIFDD1153 pKa = 3.83 YY1154 pKa = 11.32 ARR1156 pKa = 11.84 TLAQAYY1162 pKa = 9.23 EE1163 pKa = 4.37 GVLDD1167 pKa = 3.81 EE1168 pKa = 4.31 QQAKK1172 pKa = 9.91 RR1173 pKa = 11.84 RR1174 pKa = 11.84 VISATTRR1181 pKa = 11.84 QISVTRR1187 pKa = 11.84 KK1188 pKa = 6.37 TWLDD1192 pKa = 3.4 IVPLPTDD1199 pKa = 3.41 TFYY1202 pKa = 11.15 RR1203 pKa = 11.84 YY1204 pKa = 9.68 GRR1206 pKa = 11.84 ALYY1209 pKa = 10.54 KK1210 pKa = 9.63 MYY1212 pKa = 10.33 HH1213 pKa = 6.28 GLVSMPHH1220 pKa = 6.11 LEE1222 pKa = 3.84 KK1223 pKa = 10.88 ARR1225 pKa = 11.84 FVGIPPIALLDD1236 pKa = 3.65 SRR1238 pKa = 11.84 SRR1240 pKa = 11.84 NIIRR1244 pKa = 11.84 NIIVEE1249 pKa = 4.16 ASDD1252 pKa = 3.14 VEE1254 pKa = 4.31 YY1255 pKa = 10.99 ALRR1258 pKa = 11.84 VLLL1261 pKa = 4.86
Molecular weight: 143.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.369
IPC2_protein 7.746
IPC_protein 7.541
Toseland 7.278
ProMoST 8.141
Dawson 8.273
Bjellqvist 8.639
Wikipedia 8.2
Rodwell 8.302
Grimsley 7.366
Solomon 8.302
Lehninger 8.317
Nozaki 8.77
DTASelect 8.375
Thurlkill 8.419
EMBOSS 8.463
Sillero 8.726
Patrickios 4.342
IPC_peptide 8.302
IPC2_peptide 7.614
IPC2.peptide.svr19 7.719
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1678
417
1261
839.0
94.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.701 ± 0.137
1.669 ± 0.103
5.721 ± 0.092
5.423 ± 0.173
4.648 ± 0.387
6.198 ± 0.659
2.741 ± 0.475
4.827 ± 0.335
4.35 ± 0.229
7.628 ± 0.128
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.168
3.516 ± 0.143
5.006 ± 0.763
3.993 ± 0.252
7.569 ± 0.274
5.066 ± 0.12
6.853 ± 0.152
7.926 ± 0.541
1.49 ± 0.13
3.933 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here