Ziziphus jujuba (Chinese jujube) (Ziziphus sativa)
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34600 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P3Z2V6|A0A6P3Z2V6_ZIZJJ Endoglucanase OS=Ziziphus jujuba OX=326968 GN=LOC107408874 PE=3 SV=1
MM1 pKa = 7.92 AEE3 pKa = 3.69 VTYY6 pKa = 10.85 LHH8 pKa = 6.0 LHH10 pKa = 5.26 EE11 pKa = 5.51 VEE13 pKa = 5.63 DD14 pKa = 4.5 DD15 pKa = 3.61 THH17 pKa = 8.18 HH18 pKa = 7.58 PDD20 pKa = 3.48 PALSLDD26 pKa = 3.95 SIPYY30 pKa = 10.12 YY31 pKa = 11.0 DD32 pKa = 4.19 FDD34 pKa = 5.3 LYY36 pKa = 11.31 SSYY39 pKa = 11.44 PEE41 pKa = 4.77 FPPSDD46 pKa = 3.96 PSLHH50 pKa = 5.78 AHH52 pKa = 5.25 STFHH56 pKa = 7.33 EE57 pKa = 5.31 DD58 pKa = 4.46 DD59 pKa = 4.64 LLSQHH64 pKa = 6.53 EE65 pKa = 4.52 STIHH69 pKa = 5.67 IRR71 pKa = 11.84 SLEE74 pKa = 4.15 DD75 pKa = 3.44 DD76 pKa = 3.54 VSEE79 pKa = 4.87 AGSIANADD87 pKa = 3.74 LLDD90 pKa = 4.81 RR91 pKa = 11.84 EE92 pKa = 4.32 NQVNFVMDD100 pKa = 4.1 LFQQRR105 pKa = 11.84 VEE107 pKa = 3.94 QSQVMGHH114 pKa = 5.96 SDD116 pKa = 3.97 FSSEE120 pKa = 4.01 ALNDD124 pKa = 3.36 SSFGVIEE131 pKa = 4.47 GNCDD135 pKa = 2.85 VDD137 pKa = 4.47 MDD139 pKa = 5.16 GLDD142 pKa = 3.78 LDD144 pKa = 4.98 LGLGLGIDD152 pKa = 4.47 FGVEE156 pKa = 3.8 RR157 pKa = 11.84 HH158 pKa = 6.35 CLDD161 pKa = 3.49 VDD163 pKa = 3.72 NDD165 pKa = 3.92 DD166 pKa = 5.2 DD167 pKa = 4.54 EE168 pKa = 6.05 EE169 pKa = 5.99 DD170 pKa = 3.67 GFFVGRR176 pKa = 11.84 RR177 pKa = 11.84 VSGSEE182 pKa = 3.83 SGEE185 pKa = 4.19 GPSSIGGVEE194 pKa = 4.02 PFSSCVRR201 pKa = 11.84 LVGFGSDD208 pKa = 3.89 SIHH211 pKa = 7.35 VNII214 pKa = 5.29
Molecular weight: 23.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A6P4AE47|A0A6P4AE47_ZIZJJ disease resistance protein RPP13-like isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107426909 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.45 VGIEE6 pKa = 3.77 AGEE9 pKa = 3.99 VSKK12 pKa = 11.42 ASVTRR17 pKa = 11.84 EE18 pKa = 3.36 RR19 pKa = 11.84 VINKK23 pKa = 8.45 GVSVIDD29 pKa = 4.23 FILRR33 pKa = 11.84 IVACFGTLGSAIAMGTARR51 pKa = 11.84 QTFPFSTRR59 pKa = 11.84 FIRR62 pKa = 11.84 FKK64 pKa = 10.79 AVYY67 pKa = 9.56 SDD69 pKa = 4.6 LPMFSFFVIANAVVCGYY86 pKa = 10.49 LAFSLPFSILHH97 pKa = 6.68 IITRR101 pKa = 11.84 AAVKK105 pKa = 10.44 SRR107 pKa = 11.84 IVLATFDD114 pKa = 3.86 TAMMALLTAGASAAAAIASLAHH136 pKa = 6.4 NGNPSTNWFAICRR149 pKa = 11.84 QYY151 pKa = 11.61 RR152 pKa = 11.84 NFCEE156 pKa = 4.01 RR157 pKa = 11.84 VSGSLIGSFVGIVIFMLLIILALVAISRR185 pKa = 11.84 RR186 pKa = 3.77
Molecular weight: 20.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 9.604
IPC_protein 10.409
Toseland 10.57
ProMoST 10.555
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.877
Grimsley 10.745
Solomon 10.804
Lehninger 10.76
Nozaki 10.599
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.628
IPC_peptide 10.804
IPC2_peptide 9.721
IPC2.peptide.svr19 8.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28045
6555
34600
15613593
32
5234
451.3
50.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.399 ± 0.013
1.903 ± 0.006
5.274 ± 0.009
6.461 ± 0.015
4.312 ± 0.008
6.469 ± 0.012
2.427 ± 0.006
5.516 ± 0.01
6.182 ± 0.011
10.045 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.37 ± 0.005
4.697 ± 0.009
4.743 ± 0.011
3.68 ± 0.009
5.099 ± 0.01
9.088 ± 0.017
4.79 ± 0.008
6.403 ± 0.009
1.285 ± 0.004
2.848 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here