Mycobacterium virus Pukovnik

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VGN1|B3VGN1_9CAUD DNA methylase OS=Mycobacterium virus Pukovnik OX=540068 GN=82 PE=4 SV=1
MM1 pKa = 7.36IRR3 pKa = 11.84LIIAGLLILGIAQAEE18 pKa = 4.12ATIANADD25 pKa = 3.94TVNLTEE31 pKa = 4.49LRR33 pKa = 11.84TVDD36 pKa = 3.47GQDD39 pKa = 3.39YY40 pKa = 8.72PVCSEE45 pKa = 4.77EE46 pKa = 4.75DD47 pKa = 3.82CSDD50 pKa = 3.39QPGQIGLWLDD60 pKa = 3.37KK61 pKa = 10.77DD62 pKa = 3.75TGNWWLSLGEE72 pKa = 3.97TSYY75 pKa = 11.66LVVDD79 pKa = 4.04DD80 pKa = 4.11TARR83 pKa = 3.69

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VGI7|B3VGI7_9CAUD Uncharacterized protein OS=Mycobacterium virus Pukovnik OX=540068 GN=38 PE=4 SV=1
MM1 pKa = 7.9PPRR4 pKa = 11.84ASIQQTADD12 pKa = 3.35YY13 pKa = 10.31LGVSTKK19 pKa = 7.22TVRR22 pKa = 11.84NYY24 pKa = 9.75IAAGKK29 pKa = 9.64LKK31 pKa = 10.31AVRR34 pKa = 11.84LGPRR38 pKa = 11.84LIRR41 pKa = 11.84VEE43 pKa = 4.05RR44 pKa = 11.84DD45 pKa = 3.15SVEE48 pKa = 3.78ALMRR52 pKa = 11.84PIGKK56 pKa = 9.51

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

16304

37

798

185.3

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.967 ± 0.401

0.902 ± 0.131

6.219 ± 0.154

6.624 ± 0.291

3.496 ± 0.194

8.722 ± 0.49

2.085 ± 0.177

5.06 ± 0.22

4.907 ± 0.301

7.826 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.144

3.257 ± 0.203

5.496 ± 0.276

3.625 ± 0.214

6.52 ± 0.325

5.164 ± 0.225

5.9 ± 0.28

6.943 ± 0.218

2.018 ± 0.13

2.748 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski