Cognatishimia maritima
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3233 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5TV48|A0A1M5TV48_9RHOB Acyl transferase domain-containing protein OS=Cognatishimia maritima OX=870908 GN=SAMN04488044_2709 PE=3 SV=1
VV1 pKa = 6.71 GVSGQDD7 pKa = 3.17 ANGTQGVAEE16 pKa = 4.56 ALNFGNFDD24 pKa = 4.22 NIDD27 pKa = 3.11 ITGYY31 pKa = 10.02 KK32 pKa = 8.56 WADD35 pKa = 3.07 INGNGVWDD43 pKa = 4.05 EE44 pKa = 4.4 GEE46 pKa = 4.02 KK47 pKa = 10.91 GLNDD51 pKa = 3.2 FVIYY55 pKa = 10.64 LDD57 pKa = 3.72 TDD59 pKa = 3.54 EE60 pKa = 6.19 DD61 pKa = 3.98 PTNGFIRR68 pKa = 11.84 MMTTEE73 pKa = 4.12 NDD75 pKa = 3.16 GTYY78 pKa = 10.85 DD79 pKa = 3.37 GFYY82 pKa = 10.31 SFQDD86 pKa = 3.39 VTPAEE91 pKa = 4.35 INGATTLYY99 pKa = 10.15 VYY101 pKa = 10.61 EE102 pKa = 4.49 GVEE105 pKa = 3.74 PGYY108 pKa = 7.75 TQTYY112 pKa = 8.03 GGYY115 pKa = 9.9 SIDD118 pKa = 3.78 VASGAVVEE126 pKa = 4.46 GTYY129 pKa = 10.35 RR130 pKa = 11.84 EE131 pKa = 4.27 TEE133 pKa = 4.06 DD134 pKa = 4.34 GNFGNHH140 pKa = 5.17 MMEE143 pKa = 4.79 GANRR147 pKa = 11.84 TPGFWQSTLGKK158 pKa = 9.55 SLYY161 pKa = 10.89 DD162 pKa = 3.95 GDD164 pKa = 5.23 PDD166 pKa = 4.05 NQGDD170 pKa = 4.1 ANGDD174 pKa = 3.99 GIPDD178 pKa = 3.59 GNKK181 pKa = 10.22 DD182 pKa = 3.54 FEE184 pKa = 4.48 EE185 pKa = 4.62 EE186 pKa = 4.25 GWSEE190 pKa = 3.84 EE191 pKa = 4.19 DD192 pKa = 4.21 LLIKK196 pKa = 10.7 YY197 pKa = 10.13 GIDD200 pKa = 3.45 LEE202 pKa = 5.29 GGDD205 pKa = 5.05 GINDD209 pKa = 3.66 HH210 pKa = 6.44 FLVWDD215 pKa = 3.81 EE216 pKa = 4.54 NGNGVLDD223 pKa = 4.43 GDD225 pKa = 5.09 DD226 pKa = 3.12 IALTFEE232 pKa = 4.61 EE233 pKa = 4.45 LCGWVEE239 pKa = 4.11 GGGTGKK245 pKa = 9.95 RR246 pKa = 11.84 DD247 pKa = 3.6 YY248 pKa = 11.69 VEE250 pKa = 3.93 VLQRR254 pKa = 11.84 DD255 pKa = 3.63 VGATFLNLLNNSSLAGSDD273 pKa = 3.66 DD274 pKa = 4.04 VIMDD278 pKa = 3.66 GDD280 pKa = 3.86 IEE282 pKa = 4.45 DD283 pKa = 4.17 SYY285 pKa = 11.37 EE286 pKa = 3.8 AAIMFIMDD294 pKa = 3.72 NEE296 pKa = 4.35 MKK298 pKa = 10.46 GSKK301 pKa = 10.06 KK302 pKa = 10.59 AMQKK306 pKa = 9.91 DD307 pKa = 3.08 WKK309 pKa = 10.28 DD310 pKa = 3.65 YY311 pKa = 11.03 GSAAHH316 pKa = 6.7 NEE318 pKa = 3.28 LDD320 pKa = 3.53 AYY322 pKa = 10.82 NNNGQVFDD330 pKa = 4.5 AEE332 pKa = 4.52 SGTFVQTVMDD342 pKa = 5.07 GDD344 pKa = 4.36 DD345 pKa = 4.22 LSKK348 pKa = 9.8 QTAQNYY354 pKa = 9.34 FDD356 pKa = 5.76 AEE358 pKa = 3.91 DD359 pKa = 4.06 AYY361 pKa = 10.54 EE362 pKa = 4.28 LFVAA366 pKa = 5.48
Molecular weight: 39.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.567
IPC_protein 3.605
Toseland 3.376
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.528
Rodwell 3.427
Grimsley 3.274
Solomon 3.592
Lehninger 3.554
Nozaki 3.706
DTASelect 3.961
Thurlkill 3.427
EMBOSS 3.541
Sillero 3.719
Patrickios 1.278
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.682
Protein with the highest isoelectric point:
>tr|A0A1M5MMS6|A0A1M5MMS6_9RHOB 30S ribosomal protein S13 OS=Cognatishimia maritima OX=870908 GN=rpsM PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 ALSAA44 pKa = 4.07
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3233
0
3233
981079
18
2133
303.5
33.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.526 ± 0.048
0.919 ± 0.014
6.125 ± 0.035
6.123 ± 0.043
3.94 ± 0.031
8.219 ± 0.044
2.077 ± 0.022
5.487 ± 0.033
3.91 ± 0.036
9.908 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.843 ± 0.02
2.905 ± 0.022
4.753 ± 0.028
3.44 ± 0.026
6.049 ± 0.037
5.375 ± 0.027
5.441 ± 0.025
7.289 ± 0.032
1.363 ± 0.018
2.306 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here