Paenibacillus phage Xenia
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K2CYC4|A0A0K2CYC4_9CAUD Uncharacterized protein OS=Paenibacillus phage Xenia OX=1636263 GN=XENIA_69 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.05 IKK4 pKa = 10.36 YY5 pKa = 8.81 RR6 pKa = 11.84 LSIGYY11 pKa = 8.71 PGACQEE17 pKa = 4.77 DD18 pKa = 4.79 EE19 pKa = 4.58 IEE21 pKa = 4.69 IDD23 pKa = 3.85 DD24 pKa = 5.0 KK25 pKa = 11.29 EE26 pKa = 4.63 LEE28 pKa = 4.24 GLTPEE33 pKa = 4.11 EE34 pKa = 4.18 AEE36 pKa = 4.07 GRR38 pKa = 11.84 ICEE41 pKa = 4.07 IVEE44 pKa = 4.33 EE45 pKa = 4.26 YY46 pKa = 10.89 VQDD49 pKa = 5.08 FISLSWEE56 pKa = 4.02 EE57 pKa = 3.99 VEE59 pKa = 4.75 EE60 pKa = 4.21
Molecular weight: 7.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.065
IPC2_protein 3.973
IPC_protein 3.846
Toseland 3.694
ProMoST 3.91
Dawson 3.783
Bjellqvist 3.999
Wikipedia 3.643
Rodwell 3.681
Grimsley 3.605
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 3.961
Thurlkill 3.719
EMBOSS 3.656
Sillero 3.948
Patrickios 2.918
IPC_peptide 3.77
IPC2_peptide 3.935
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|A0A0K2CYZ9|A0A0K2CYZ9_9CAUD Uncharacterized protein OS=Paenibacillus phage Xenia OX=1636263 GN=XENIA_32 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.25 RR3 pKa = 11.84 YY4 pKa = 6.87 TLQYY8 pKa = 8.83 AGSVKK13 pKa = 9.98 RR14 pKa = 11.84 SHH16 pKa = 6.49 IPYY19 pKa = 8.91 VNYY22 pKa = 10.45 VILLGSNVHH31 pKa = 6.85 RR32 pKa = 11.84 IINGSTGKK40 pKa = 10.14 KK41 pKa = 9.42 VLMRR45 pKa = 11.84 SLIKK49 pKa = 10.51 RR50 pKa = 11.84 CFPP53 pKa = 4.28
Molecular weight: 6.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 10.409
IPC_protein 11.023
Toseland 10.935
ProMoST 10.687
Dawson 11.052
Bjellqvist 10.789
Wikipedia 11.286
Rodwell 11.359
Grimsley 11.111
Solomon 11.169
Lehninger 11.125
Nozaki 10.921
DTASelect 10.774
Thurlkill 10.95
EMBOSS 11.345
Sillero 10.979
Patrickios 11.125
IPC_peptide 11.169
IPC2_peptide 9.838
IPC2.peptide.svr19 7.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
12706
36
878
165.0
18.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.532 ± 0.435
0.897 ± 0.111
5.58 ± 0.183
8.106 ± 0.395
3.55 ± 0.166
5.887 ± 0.36
1.81 ± 0.174
7.13 ± 0.329
9.067 ± 0.318
8.02 ± 0.236
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.006 ± 0.166
4.785 ± 0.194
3.203 ± 0.182
4.368 ± 0.21
5.218 ± 0.406
6.312 ± 0.274
5.344 ± 0.265
6.052 ± 0.192
1.362 ± 0.184
3.77 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here