Amino acid dipepetide frequency for Paenibacillus phage Xenia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.352AlaAla: 5.352 ± 1.02
0.787AlaCys: 0.787 ± 0.232
3.935AlaAsp: 3.935 ± 0.625
6.061AlaGlu: 6.061 ± 0.713
1.968AlaPhe: 1.968 ± 0.318
4.565AlaGly: 4.565 ± 0.646
1.023AlaHis: 1.023 ± 0.296
4.723AlaIle: 4.723 ± 0.832
5.274AlaLys: 5.274 ± 0.722
5.51AlaLeu: 5.51 ± 0.688
1.259AlaMet: 1.259 ± 0.295
2.755AlaAsn: 2.755 ± 0.419
1.495AlaPro: 1.495 ± 0.272
2.519AlaGln: 2.519 ± 0.405
2.283AlaArg: 2.283 ± 0.43
4.486AlaSer: 4.486 ± 0.674
3.699AlaThr: 3.699 ± 0.841
4.093AlaVal: 4.093 ± 0.5
1.181AlaTrp: 1.181 ± 0.267
2.361AlaTyr: 2.361 ± 0.472
0.0AlaXaa: 0.0 ± 0.0
Cys
0.472CysAla: 0.472 ± 0.208
0.079CysCys: 0.079 ± 0.09
0.472CysAsp: 0.472 ± 0.185
0.394CysGlu: 0.394 ± 0.211
0.394CysPhe: 0.394 ± 0.173
0.63CysGly: 0.63 ± 0.215
0.079CysHis: 0.079 ± 0.075
0.945CysIle: 0.945 ± 0.265
1.259CysLys: 1.259 ± 0.454
0.866CysLeu: 0.866 ± 0.273
0.157CysMet: 0.157 ± 0.103
0.236CysAsn: 0.236 ± 0.119
0.472CysPro: 0.472 ± 0.235
0.472CysGln: 0.472 ± 0.207
0.394CysArg: 0.394 ± 0.168
0.787CysSer: 0.787 ± 0.225
0.157CysThr: 0.157 ± 0.101
0.394CysVal: 0.394 ± 0.162
0.079CysTrp: 0.079 ± 0.069
0.236CysTyr: 0.236 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
3.935AspAla: 3.935 ± 0.56
0.236AspCys: 0.236 ± 0.143
4.25AspAsp: 4.25 ± 0.597
3.778AspGlu: 3.778 ± 0.6
3.384AspPhe: 3.384 ± 0.52
3.463AspGly: 3.463 ± 0.502
0.787AspHis: 0.787 ± 0.252
2.991AspIle: 2.991 ± 0.501
3.857AspLys: 3.857 ± 0.585
5.274AspLeu: 5.274 ± 0.575
1.732AspMet: 1.732 ± 0.423
2.046AspAsn: 2.046 ± 0.399
1.732AspPro: 1.732 ± 0.406
2.361AspGln: 2.361 ± 0.413
2.912AspArg: 2.912 ± 0.499
3.148AspSer: 3.148 ± 0.468
2.676AspThr: 2.676 ± 0.475
4.093AspVal: 4.093 ± 0.591
1.023AspTrp: 1.023 ± 0.295
2.125AspTyr: 2.125 ± 0.356
0.0AspXaa: 0.0 ± 0.0
Glu
5.037GluAla: 5.037 ± 0.735
0.236GluCys: 0.236 ± 0.117
4.25GluAsp: 4.25 ± 0.63
5.903GluGlu: 5.903 ± 0.875
2.44GluPhe: 2.44 ± 0.367
4.014GluGly: 4.014 ± 0.514
1.259GluHis: 1.259 ± 0.399
6.533GluIle: 6.533 ± 0.984
8.186GluLys: 8.186 ± 0.892
7.005GluLeu: 7.005 ± 0.667
4.723GluMet: 4.723 ± 0.597
3.463GluAsn: 3.463 ± 0.618
1.968GluPro: 1.968 ± 0.34
4.014GluGln: 4.014 ± 0.677
3.857GluArg: 3.857 ± 0.607
3.621GluSer: 3.621 ± 0.503
4.644GluThr: 4.644 ± 0.704
5.982GluVal: 5.982 ± 0.748
0.945GluTrp: 0.945 ± 0.285
2.991GluTyr: 2.991 ± 0.506
0.0GluXaa: 0.0 ± 0.0
Phe
1.81PheAla: 1.81 ± 0.402
0.866PheCys: 0.866 ± 0.259
2.125PheAsp: 2.125 ± 0.367
3.384PheGlu: 3.384 ± 0.574
0.708PhePhe: 0.708 ± 0.281
2.125PheGly: 2.125 ± 0.349
1.023PheHis: 1.023 ± 0.341
2.991PheIle: 2.991 ± 0.394
2.597PheLys: 2.597 ± 0.476
2.519PheLeu: 2.519 ± 0.405
0.866PheMet: 0.866 ± 0.264
2.204PheAsn: 2.204 ± 0.346
0.63PhePro: 0.63 ± 0.239
1.574PheGln: 1.574 ± 0.351
1.023PheArg: 1.023 ± 0.298
2.676PheSer: 2.676 ± 0.463
1.81PheThr: 1.81 ± 0.381
2.597PheVal: 2.597 ± 0.459
0.708PheTrp: 0.708 ± 0.262
1.259PheTyr: 1.259 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
3.07GlyAla: 3.07 ± 0.51
0.708GlyCys: 0.708 ± 0.231
2.676GlyAsp: 2.676 ± 0.522
4.88GlyGlu: 4.88 ± 0.753
1.968GlyPhe: 1.968 ± 0.435
3.463GlyGly: 3.463 ± 0.557
0.787GlyHis: 0.787 ± 0.23
5.352GlyIle: 5.352 ± 0.853
6.454GlyLys: 6.454 ± 0.67
4.644GlyLeu: 4.644 ± 0.901
1.81GlyMet: 1.81 ± 0.619
3.148GlyAsn: 3.148 ± 0.512
0.945GlyPro: 0.945 ± 0.253
2.361GlyGln: 2.361 ± 0.446
3.542GlyArg: 3.542 ± 0.484
3.306GlySer: 3.306 ± 0.593
2.597GlyThr: 2.597 ± 0.509
3.227GlyVal: 3.227 ± 0.502
1.102GlyTrp: 1.102 ± 0.246
2.834GlyTyr: 2.834 ± 0.488
0.0GlyXaa: 0.0 ± 0.0
His
1.259HisAla: 1.259 ± 0.357
0.394HisCys: 0.394 ± 0.192
0.472HisAsp: 0.472 ± 0.206
1.181HisGlu: 1.181 ± 0.291
0.708HisPhe: 0.708 ± 0.212
0.551HisGly: 0.551 ± 0.175
0.551HisHis: 0.551 ± 0.196
1.889HisIle: 1.889 ± 0.486
1.338HisLys: 1.338 ± 0.33
1.968HisLeu: 1.968 ± 0.406
0.63HisMet: 0.63 ± 0.244
0.945HisAsn: 0.945 ± 0.251
0.866HisPro: 0.866 ± 0.27
0.472HisGln: 0.472 ± 0.186
1.338HisArg: 1.338 ± 0.313
1.181HisSer: 1.181 ± 0.354
0.394HisThr: 0.394 ± 0.193
1.102HisVal: 1.102 ± 0.28
0.394HisTrp: 0.394 ± 0.161
0.472HisTyr: 0.472 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
4.172IleAla: 4.172 ± 0.661
0.787IleCys: 0.787 ± 0.246
4.801IleAsp: 4.801 ± 0.737
6.533IleGlu: 6.533 ± 0.612
3.07IlePhe: 3.07 ± 0.467
3.542IleGly: 3.542 ± 0.613
1.574IleHis: 1.574 ± 0.351
3.227IleIle: 3.227 ± 0.691
7.399IleLys: 7.399 ± 0.933
5.746IleLeu: 5.746 ± 0.76
1.732IleMet: 1.732 ± 0.361
3.542IleAsn: 3.542 ± 0.531
2.834IlePro: 2.834 ± 0.343
3.07IleGln: 3.07 ± 0.506
3.778IleArg: 3.778 ± 0.61
3.463IleSer: 3.463 ± 0.609
4.014IleThr: 4.014 ± 0.447
4.801IleVal: 4.801 ± 0.755
0.551IleTrp: 0.551 ± 0.25
2.597IleTyr: 2.597 ± 0.46
0.0IleXaa: 0.0 ± 0.0
Lys
6.375LysAla: 6.375 ± 0.698
0.315LysCys: 0.315 ± 0.165
4.408LysAsp: 4.408 ± 0.463
9.13LysGlu: 9.13 ± 0.858
1.889LysPhe: 1.889 ± 0.244
5.195LysGly: 5.195 ± 0.651
1.181LysHis: 1.181 ± 0.243
5.431LysIle: 5.431 ± 0.579
8.973LysLys: 8.973 ± 1.117
6.769LysLeu: 6.769 ± 0.588
3.306LysMet: 3.306 ± 0.511
3.935LysAsn: 3.935 ± 0.603
2.755LysPro: 2.755 ± 0.466
4.565LysGln: 4.565 ± 0.577
6.139LysArg: 6.139 ± 0.72
5.352LysSer: 5.352 ± 0.609
5.51LysThr: 5.51 ± 0.668
5.037LysVal: 5.037 ± 0.641
1.259LysTrp: 1.259 ± 0.261
3.148LysTyr: 3.148 ± 0.548
0.0LysXaa: 0.0 ± 0.0
Leu
6.297LeuAla: 6.297 ± 0.695
0.866LeuCys: 0.866 ± 0.289
4.565LeuAsp: 4.565 ± 0.507
6.69LeuGlu: 6.69 ± 0.84
3.306LeuPhe: 3.306 ± 0.557
4.644LeuGly: 4.644 ± 0.664
1.732LeuHis: 1.732 ± 0.361
5.195LeuIle: 5.195 ± 0.445
6.848LeuLys: 6.848 ± 0.715
5.116LeuLeu: 5.116 ± 0.783
1.574LeuMet: 1.574 ± 0.38
4.093LeuAsn: 4.093 ± 0.482
2.912LeuPro: 2.912 ± 0.494
4.25LeuGln: 4.25 ± 0.541
3.542LeuArg: 3.542 ± 0.568
6.375LeuSer: 6.375 ± 0.589
4.88LeuThr: 4.88 ± 0.557
3.384LeuVal: 3.384 ± 0.452
0.945LeuTrp: 0.945 ± 0.315
2.991LeuTyr: 2.991 ± 0.51
0.0LeuXaa: 0.0 ± 0.0
Met
1.81MetAla: 1.81 ± 0.308
0.079MetCys: 0.079 ± 0.071
1.495MetAsp: 1.495 ± 0.287
2.44MetGlu: 2.44 ± 0.401
1.023MetPhe: 1.023 ± 0.256
1.495MetGly: 1.495 ± 0.298
0.315MetHis: 0.315 ± 0.143
2.519MetIle: 2.519 ± 0.48
3.857MetLys: 3.857 ± 0.55
2.361MetLeu: 2.361 ± 0.469
0.63MetMet: 0.63 ± 0.228
2.125MetAsn: 2.125 ± 0.377
1.181MetPro: 1.181 ± 0.305
1.181MetGln: 1.181 ± 0.264
1.102MetArg: 1.102 ± 0.291
2.676MetSer: 2.676 ± 0.458
1.653MetThr: 1.653 ± 0.382
1.023MetVal: 1.023 ± 0.252
0.236MetTrp: 0.236 ± 0.152
0.866MetTyr: 0.866 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
2.597AsnAla: 2.597 ± 0.458
0.394AsnCys: 0.394 ± 0.155
2.125AsnAsp: 2.125 ± 0.531
4.172AsnGlu: 4.172 ± 0.678
1.338AsnPhe: 1.338 ± 0.304
3.699AsnGly: 3.699 ± 0.522
0.866AsnHis: 0.866 ± 0.249
3.07AsnIle: 3.07 ± 0.502
4.014AsnLys: 4.014 ± 0.493
3.857AsnLeu: 3.857 ± 0.552
1.259AsnMet: 1.259 ± 0.402
2.125AsnAsn: 2.125 ± 0.521
2.519AsnPro: 2.519 ± 0.474
1.889AsnGln: 1.889 ± 0.351
4.014AsnArg: 4.014 ± 0.756
2.834AsnSer: 2.834 ± 0.487
2.125AsnThr: 2.125 ± 0.346
2.755AsnVal: 2.755 ± 0.44
0.472AsnTrp: 0.472 ± 0.199
1.732AsnTyr: 1.732 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
2.283ProAla: 2.283 ± 0.393
0.236ProCys: 0.236 ± 0.144
1.574ProAsp: 1.574 ± 0.369
2.755ProGlu: 2.755 ± 0.763
1.102ProPhe: 1.102 ± 0.285
2.125ProGly: 2.125 ± 0.402
0.708ProHis: 0.708 ± 0.243
2.283ProIle: 2.283 ± 0.462
3.07ProLys: 3.07 ± 0.6
2.204ProLeu: 2.204 ± 0.437
0.945ProMet: 0.945 ± 0.242
1.968ProAsn: 1.968 ± 0.362
0.945ProPro: 0.945 ± 0.272
1.023ProGln: 1.023 ± 0.269
1.259ProArg: 1.259 ± 0.31
2.519ProSer: 2.519 ± 0.59
1.338ProThr: 1.338 ± 0.243
1.889ProVal: 1.889 ± 0.479
0.472ProTrp: 0.472 ± 0.18
1.338ProTyr: 1.338 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
3.463GlnAla: 3.463 ± 0.498
0.0GlnCys: 0.0 ± 0.0
1.968GlnAsp: 1.968 ± 0.301
3.778GlnGlu: 3.778 ± 0.599
1.495GlnPhe: 1.495 ± 0.346
2.283GlnGly: 2.283 ± 0.489
1.181GlnHis: 1.181 ± 0.28
3.621GlnIle: 3.621 ± 0.681
2.912GlnLys: 2.912 ± 0.653
3.857GlnLeu: 3.857 ± 0.619
1.968GlnMet: 1.968 ± 0.353
1.81GlnAsn: 1.81 ± 0.316
1.732GlnPro: 1.732 ± 0.508
2.125GlnGln: 2.125 ± 0.444
2.597GlnArg: 2.597 ± 0.453
2.519GlnSer: 2.519 ± 0.41
2.204GlnThr: 2.204 ± 0.358
2.125GlnVal: 2.125 ± 0.428
0.472GlnTrp: 0.472 ± 0.213
1.574GlnTyr: 1.574 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
2.519ArgAla: 2.519 ± 0.457
0.63ArgCys: 0.63 ± 0.239
3.07ArgAsp: 3.07 ± 0.452
4.408ArgGlu: 4.408 ± 0.599
1.732ArgPhe: 1.732 ± 0.372
2.912ArgGly: 2.912 ± 0.513
1.181ArgHis: 1.181 ± 0.271
3.778ArgIle: 3.778 ± 0.622
6.297ArgLys: 6.297 ± 0.721
4.25ArgLeu: 4.25 ± 0.578
1.574ArgMet: 1.574 ± 0.349
2.597ArgAsn: 2.597 ± 0.467
1.338ArgPro: 1.338 ± 0.339
2.912ArgGln: 2.912 ± 0.422
3.227ArgArg: 3.227 ± 0.513
2.283ArgSer: 2.283 ± 0.418
2.204ArgThr: 2.204 ± 0.442
2.597ArgVal: 2.597 ± 0.464
0.787ArgTrp: 0.787 ± 0.263
1.889ArgTyr: 1.889 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
5.431SerAla: 5.431 ± 0.94
0.472SerCys: 0.472 ± 0.191
3.935SerAsp: 3.935 ± 0.572
4.565SerGlu: 4.565 ± 0.667
2.755SerPhe: 2.755 ± 0.45
3.857SerGly: 3.857 ± 0.632
1.023SerHis: 1.023 ± 0.348
5.903SerIle: 5.903 ± 0.754
4.25SerLys: 4.25 ± 0.692
5.195SerLeu: 5.195 ± 0.617
1.574SerMet: 1.574 ± 0.342
2.204SerAsn: 2.204 ± 0.422
1.889SerPro: 1.889 ± 0.319
2.755SerGln: 2.755 ± 0.506
3.384SerArg: 3.384 ± 0.545
4.093SerSer: 4.093 ± 0.725
3.306SerThr: 3.306 ± 0.512
4.093SerVal: 4.093 ± 0.831
0.472SerTrp: 0.472 ± 0.188
1.968SerTyr: 1.968 ± 0.395
0.0SerXaa: 0.0 ± 0.0
Thr
3.621ThrAla: 3.621 ± 0.702
0.472ThrCys: 0.472 ± 0.163
2.597ThrAsp: 2.597 ± 0.345
3.07ThrGlu: 3.07 ± 0.413
1.81ThrPhe: 1.81 ± 0.378
4.014ThrGly: 4.014 ± 0.515
1.023ThrHis: 1.023 ± 0.317
3.542ThrIle: 3.542 ± 0.593
4.723ThrLys: 4.723 ± 0.577
4.329ThrLeu: 4.329 ± 0.725
1.417ThrMet: 1.417 ± 0.355
1.968ThrAsn: 1.968 ± 0.326
2.597ThrPro: 2.597 ± 0.493
2.046ThrGln: 2.046 ± 0.366
2.283ThrArg: 2.283 ± 0.381
3.935ThrSer: 3.935 ± 0.609
2.834ThrThr: 2.834 ± 0.488
4.25ThrVal: 4.25 ± 0.597
0.315ThrTrp: 0.315 ± 0.123
2.597ThrTyr: 2.597 ± 0.443
0.0ThrXaa: 0.0 ± 0.0
Val
3.621ValAla: 3.621 ± 0.551
0.63ValCys: 0.63 ± 0.23
3.384ValAsp: 3.384 ± 0.595
4.565ValGlu: 4.565 ± 0.786
2.597ValPhe: 2.597 ± 0.403
2.755ValGly: 2.755 ± 0.553
0.708ValHis: 0.708 ± 0.294
3.621ValIle: 3.621 ± 0.464
5.352ValLys: 5.352 ± 0.645
4.408ValLeu: 4.408 ± 0.509
1.81ValMet: 1.81 ± 0.419
2.991ValAsn: 2.991 ± 0.599
1.495ValPro: 1.495 ± 0.333
1.889ValGln: 1.889 ± 0.364
3.306ValArg: 3.306 ± 0.502
5.274ValSer: 5.274 ± 0.567
4.408ValThr: 4.408 ± 0.648
3.778ValVal: 3.778 ± 0.538
1.732ValTrp: 1.732 ± 0.889
2.204ValTyr: 2.204 ± 0.414
0.0ValXaa: 0.0 ± 0.0
Trp
0.551TrpAla: 0.551 ± 0.202
0.157TrpCys: 0.157 ± 0.118
0.551TrpAsp: 0.551 ± 0.303
0.787TrpGlu: 0.787 ± 0.221
0.787TrpPhe: 0.787 ± 0.214
1.181TrpGly: 1.181 ± 0.266
0.63TrpHis: 0.63 ± 0.205
0.787TrpIle: 0.787 ± 0.216
1.102TrpLys: 1.102 ± 0.311
1.338TrpLeu: 1.338 ± 0.286
0.236TrpMet: 0.236 ± 0.138
1.732TrpAsn: 1.732 ± 1.01
0.315TrpPro: 0.315 ± 0.243
0.63TrpGln: 0.63 ± 0.184
0.315TrpArg: 0.315 ± 0.156
0.63TrpSer: 0.63 ± 0.203
0.472TrpThr: 0.472 ± 0.225
0.63TrpVal: 0.63 ± 0.194
0.157TrpTrp: 0.157 ± 0.101
0.63TrpTyr: 0.63 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.653TyrAla: 1.653 ± 0.373
0.63TyrCys: 0.63 ± 0.253
3.148TyrAsp: 3.148 ± 0.466
2.597TyrGlu: 2.597 ± 0.427
1.023TyrPhe: 1.023 ± 0.261
2.361TyrGly: 2.361 ± 0.481
0.472TyrHis: 0.472 ± 0.196
2.912TyrIle: 2.912 ± 0.499
2.912TyrLys: 2.912 ± 0.382
2.991TyrLeu: 2.991 ± 0.586
0.787TyrMet: 0.787 ± 0.257
1.968TyrAsn: 1.968 ± 0.41
1.417TyrPro: 1.417 ± 0.427
1.574TyrGln: 1.574 ± 0.269
1.889TyrArg: 1.889 ± 0.365
1.968TyrSer: 1.968 ± 0.458
2.519TyrThr: 2.519 ± 0.449
2.676TyrVal: 2.676 ± 0.502
0.315TyrTrp: 0.315 ± 0.166
1.889TyrTyr: 1.889 ± 0.42
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (12706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski