Dahlia mosaic virus
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7THB7|Q7THB7_9VIRU Aphid transmission protein OS=Dahlia mosaic virus OX=213888 PE=3 SV=1
MM1 pKa = 6.94 TTIKK5 pKa = 10.69 DD6 pKa = 3.39 LAALIEE12 pKa = 4.16 GLRR15 pKa = 11.84 EE16 pKa = 4.1 EE17 pKa = 4.11 ISKK20 pKa = 10.81 LKK22 pKa = 11.11 AEE24 pKa = 4.42 ITSKK28 pKa = 10.28 DD29 pKa = 3.25 TGPTDD34 pKa = 3.46 NTALEE39 pKa = 4.18 SLGAKK44 pKa = 9.65 IIKK47 pKa = 9.47 EE48 pKa = 4.26 LSDD51 pKa = 4.32 KK52 pKa = 10.16 MDD54 pKa = 3.27 KK55 pKa = 11.11 CEE57 pKa = 3.9 CTEE60 pKa = 3.91 RR61 pKa = 11.84 LKK63 pKa = 11.13 KK64 pKa = 10.01 EE65 pKa = 4.06 LQEE68 pKa = 4.11 QPDD71 pKa = 3.52 KK72 pKa = 11.32 GKK74 pKa = 10.8 QIVSPDD80 pKa = 3.51 KK81 pKa = 10.96 QEE83 pKa = 4.09 SSTPSSNFTRR93 pKa = 11.84 YY94 pKa = 9.74 SYY96 pKa = 10.92 PNWNVGNASLGSSGNPDD113 pKa = 3.37 ALKK116 pKa = 10.07 WPPVV120 pKa = 3.35
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.243
IPC2_protein 5.385
IPC_protein 5.169
Toseland 5.016
ProMoST 5.258
Dawson 5.092
Bjellqvist 5.245
Wikipedia 4.94
Rodwell 5.003
Grimsley 4.927
Solomon 5.092
Lehninger 5.041
Nozaki 5.207
DTASelect 5.334
Thurlkill 5.016
EMBOSS 4.965
Sillero 5.27
Patrickios 4.571
IPC_peptide 5.105
IPC2_peptide 5.258
IPC2.peptide.svr19 5.449
Protein with the highest isoelectric point:
>tr|Q7TDA0|Q7TDA0_9VIRU Inclusion body matrix protein OS=Dahlia mosaic virus OX=213888 GN=VI PE=3 SV=1
MM1 pKa = 7.5 GIKK4 pKa = 9.14 YY5 pKa = 8.32 TMSMHH10 pKa = 5.13 QQQLKK15 pKa = 10.86 ADD17 pKa = 4.42 LKK19 pKa = 10.59 QINSEE24 pKa = 4.04 IIYY27 pKa = 10.06 VNQQIKK33 pKa = 10.19 IIKK36 pKa = 9.07 QNPKK40 pKa = 10.47 GNMFSSRR47 pKa = 11.84 VKK49 pKa = 10.21 SLRR52 pKa = 11.84 INSLKK57 pKa = 10.71 ADD59 pKa = 3.78 LTNLFKK65 pKa = 10.54 QRR67 pKa = 11.84 KK68 pKa = 7.82 LLEE71 pKa = 4.34 AQIHH75 pKa = 5.2
Molecular weight: 8.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.142
IPC2_protein 9.677
IPC_protein 9.677
Toseland 10.643
ProMoST 10.101
Dawson 10.716
Bjellqvist 10.292
Wikipedia 10.818
Rodwell 11.447
Grimsley 10.745
Solomon 10.745
Lehninger 10.73
Nozaki 10.584
DTASelect 10.292
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.628
Patrickios 11.199
IPC_peptide 10.76
IPC2_peptide 8.682
IPC2.peptide.svr19 8.671
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2325
38
609
290.6
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.473 ± 0.53
1.892 ± 0.3
5.849 ± 0.519
7.656 ± 0.558
3.828 ± 0.278
4.172 ± 0.379
1.849 ± 0.266
7.613 ± 0.628
10.581 ± 0.767
8.602 ± 0.579
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.892 ± 0.283
5.591 ± 0.327
4.516 ± 0.504
5.376 ± 0.622
3.957 ± 0.251
7.871 ± 0.915
5.376 ± 0.465
4.688 ± 0.501
1.032 ± 0.15
3.183 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here