Arachis duranensis (Wild peanut)
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44786 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P5NAA3|A0A6P5NAA3_ARADU protein FAM133 isoform X1 OS=Arachis duranensis OX=130453 GN=LOC107477545 PE=4 SV=1
MM1 pKa = 7.77 DD2 pKa = 3.54 VKK4 pKa = 11.08 GNNVTPDD11 pKa = 3.76 FSSYY15 pKa = 11.15 ILFEE19 pKa = 4.3 ASGDD23 pKa = 3.92 SEE25 pKa = 4.75 EE26 pKa = 6.19 ADD28 pKa = 3.27 FDD30 pKa = 4.55 HH31 pKa = 6.94 NKK33 pKa = 8.6 MVCEE37 pKa = 4.39 ISRR40 pKa = 11.84 VGSYY44 pKa = 7.33 VHH46 pKa = 6.96 HH47 pKa = 6.22 GHH49 pKa = 6.84 EE50 pKa = 5.63 DD51 pKa = 3.52 YY52 pKa = 11.55 DD53 pKa = 5.52 DD54 pKa = 6.14 DD55 pKa = 7.24 DD56 pKa = 5.42 DD57 pKa = 6.0 ALSCSYY63 pKa = 10.96 DD64 pKa = 3.59 RR65 pKa = 11.84 SDD67 pKa = 5.13 YY68 pKa = 11.59 DD69 pKa = 5.26 DD70 pKa = 4.85 DD71 pKa = 4.18 ACNVAEE77 pKa = 4.81 VNWDD81 pKa = 4.18 DD82 pKa = 6.48 DD83 pKa = 5.97 DD84 pKa = 7.68 DD85 pKa = 7.44 DD86 pKa = 7.49 DD87 pKa = 7.49 DD88 pKa = 6.86 DD89 pKa = 5.28 GEE91 pKa = 4.38 KK92 pKa = 10.43 KK93 pKa = 10.59 DD94 pKa = 3.84 VVFGTSYY101 pKa = 10.97 CDD103 pKa = 3.56 EE104 pKa = 4.45 EE105 pKa = 6.15 DD106 pKa = 3.67 EE107 pKa = 4.75 VLQEE111 pKa = 3.83 QHH113 pKa = 5.93 PTRR116 pKa = 11.84 SFVSFASNQEE126 pKa = 4.17 SLDD129 pKa = 3.6 EE130 pKa = 4.11 MEE132 pKa = 4.68 KK133 pKa = 10.72 NRR135 pKa = 11.84 LFWEE139 pKa = 4.55 ACLASS144 pKa = 3.65
Molecular weight: 16.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.376
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.821
Patrickios 1.163
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A6P4BCF6|A0A6P4BCF6_ARADU uncharacterized protein LOC107461533 OS=Arachis duranensis OX=130453 GN=LOC107461533 PE=4 SV=1
MM1 pKa = 7.29 AAWVSNYY8 pKa = 9.79 NPSATRR14 pKa = 11.84 TLSVARR20 pKa = 11.84 PPPPSSPSLLPLPSTTTQPPPTPPGRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 PPRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 HH55 pKa = 5.6 LPPLSSSLLSSSFFFPPSSATAHH78 pKa = 5.75 LRR80 pKa = 11.84 ATARR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 HH87 pKa = 6.05 RR88 pKa = 11.84 PTATPLQPATPPLPLPPATSLPHH111 pKa = 6.45 SPPPPTRR118 pKa = 11.84 FRR120 pKa = 11.84 NTQTLLSVRR129 pKa = 11.84 SFSS132 pKa = 3.68
Molecular weight: 14.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.497
IPC2_protein 11.272
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.384
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.106
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
34312
10474
44786
19145231
37
5423
427.5
47.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.506 ± 0.011
1.932 ± 0.005
5.353 ± 0.007
6.483 ± 0.012
4.223 ± 0.007
6.203 ± 0.011
2.572 ± 0.005
5.434 ± 0.009
6.256 ± 0.012
9.686 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.456 ± 0.005
4.72 ± 0.008
4.823 ± 0.01
3.769 ± 0.008
5.279 ± 0.009
8.803 ± 0.012
4.889 ± 0.006
6.427 ± 0.007
1.298 ± 0.004
2.875 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here