Carnobacterium maltaromaticum LMA28
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K8EQM0|K8EQM0_CARML Uncharacterized protein OS=Carnobacterium maltaromaticum LMA28 OX=1234679 GN=ytcE PE=4 SV=1
MM1 pKa = 7.2 SHH3 pKa = 7.02 DD4 pKa = 4.21 HH5 pKa = 6.1 NHH7 pKa = 7.06 DD8 pKa = 4.35 DD9 pKa = 3.43 EE10 pKa = 5.39 HH11 pKa = 7.77 HH12 pKa = 6.36 DD13 pKa = 4.01 HH14 pKa = 6.2 EE15 pKa = 6.37 HH16 pKa = 5.41 ITIIDD21 pKa = 3.61 EE22 pKa = 4.39 NGNEE26 pKa = 4.05 EE27 pKa = 4.39 LYY29 pKa = 10.83 EE30 pKa = 3.88 ILFTFDD36 pKa = 2.91 SDD38 pKa = 5.19 DD39 pKa = 3.56 YY40 pKa = 10.6 GKK42 pKa = 10.79 SYY44 pKa = 11.08 VLVYY48 pKa = 9.67 PAGIPEE54 pKa = 4.27 GEE56 pKa = 4.32 EE57 pKa = 3.73 IEE59 pKa = 4.44 LQAFSYY65 pKa = 10.85 VEE67 pKa = 4.1 QEE69 pKa = 4.33 NGDD72 pKa = 3.74 QGDD75 pKa = 4.34 LKK77 pKa = 10.79 PIEE80 pKa = 4.47 TDD82 pKa = 3.6 EE83 pKa = 4.11 EE84 pKa = 4.18 WDD86 pKa = 3.67 MIEE89 pKa = 4.26 EE90 pKa = 4.12 VLNTFMADD98 pKa = 3.3 EE99 pKa = 5.13 DD100 pKa = 4.55 GEE102 pKa = 4.34
Molecular weight: 11.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.528
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.884
Wikipedia 3.605
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.579
EMBOSS 3.617
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|K8EFY6|K8EFY6_CARML Putative membrane protein OS=Carnobacterium maltaromaticum LMA28 OX=1234679 GN=BN424_1311 PE=4 SV=2
MM1 pKa = 7.92 PKK3 pKa = 10.12 QKK5 pKa = 7.54 THH7 pKa = 6.73 RR8 pKa = 11.84 GSAKK12 pKa = 9.57 RR13 pKa = 11.84 FKK15 pKa = 9.81 RR16 pKa = 11.84 TGGGGLKK23 pKa = 9.52 RR24 pKa = 11.84 HH25 pKa = 6.2 HH26 pKa = 7.17 AFTSHH31 pKa = 5.07 MFANKK36 pKa = 8.87 SQKK39 pKa = 8.6 QKK41 pKa = 10.85 RR42 pKa = 11.84 KK43 pKa = 8.92 LRR45 pKa = 11.84 KK46 pKa = 9.37 AGMVSKK52 pKa = 11.18 GDD54 pKa = 3.75 FKK56 pKa = 11.14 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLSQMKK66 pKa = 9.9
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.804
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.471
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.193
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.978
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3539
0
3539
1025100
37
2288
289.7
32.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.01 ± 0.048
0.582 ± 0.011
5.139 ± 0.035
7.386 ± 0.047
4.598 ± 0.034
6.601 ± 0.04
1.652 ± 0.019
8.0 ± 0.045
7.281 ± 0.036
9.864 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.598 ± 0.022
4.994 ± 0.037
3.395 ± 0.026
3.813 ± 0.028
3.394 ± 0.033
6.291 ± 0.036
6.066 ± 0.048
6.818 ± 0.037
0.903 ± 0.015
3.615 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here