Mycobacterium virus lockley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VG94|B3VG94_9CAUD Uncharacterized protein OS=Mycobacterium virus lockley OX=540067 GN=34 PE=4 SV=1
MM1 pKa = 7.96SEE3 pKa = 4.32EE4 pKa = 3.94MQQLRR9 pKa = 11.84KK10 pKa = 10.09EE11 pKa = 3.89IFTTYY16 pKa = 10.61VEE18 pKa = 4.35YY19 pKa = 10.84EE20 pKa = 4.01PAGPHH25 pKa = 6.01QGPGWMGFGLNSICWGAWQGWVGNLGGYY53 pKa = 9.1IAVIFYY59 pKa = 10.54NSEE62 pKa = 4.22DD63 pKa = 3.66GSVIAVTLNNFSAHH77 pKa = 6.4ADD79 pKa = 3.56AVDD82 pKa = 4.16LFYY85 pKa = 11.09QIAYY89 pKa = 10.29LLDD92 pKa = 4.14PEE94 pKa = 4.79STGHH98 pKa = 6.76RR99 pKa = 11.84DD100 pKa = 2.94WIFRR104 pKa = 11.84PDD106 pKa = 3.47PAEE109 pKa = 4.9DD110 pKa = 3.62EE111 pKa = 5.0DD112 pKa = 4.22EE113 pKa = 4.53VRR115 pKa = 11.84DD116 pKa = 3.63PTLYY120 pKa = 9.89LTVEE124 pKa = 4.33STGDD128 pKa = 3.38NQIPADD134 pKa = 3.87VPFEE138 pKa = 3.98II139 pKa = 5.71

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VGA1|B3VGA1_9CAUD Uncharacterized protein OS=Mycobacterium virus lockley OX=540067 GN=41 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84EE3 pKa = 3.65LWGNDD8 pKa = 2.41ARR10 pKa = 11.84KK11 pKa = 8.79WLIRR15 pKa = 11.84KK16 pKa = 9.05SPHH19 pKa = 3.88TQEE22 pKa = 5.54RR23 pKa = 11.84IVFPSVGSFYY33 pKa = 11.27GVITFHH39 pKa = 7.49PDD41 pKa = 3.05YY42 pKa = 10.5EE43 pKa = 4.37SARR46 pKa = 11.84ADD48 pKa = 4.76FIRR51 pKa = 11.84QTRR54 pKa = 11.84RR55 pKa = 11.84PP56 pKa = 3.6

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

15824

22

826

175.8

19.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.808 ± 0.424

0.67 ± 0.111

6.699 ± 0.188

6.642 ± 0.294

3.172 ± 0.173

8.696 ± 0.502

1.991 ± 0.16

4.91 ± 0.213

4.215 ± 0.284

8.544 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.129

3.185 ± 0.135

5.549 ± 0.244

3.267 ± 0.177

6.225 ± 0.363

5.921 ± 0.281

6.199 ± 0.28

7.242 ± 0.211

1.978 ± 0.13

3.021 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski