Clostridiales bacterium KLE1615

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3669 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A176U395|A0A176U395_9FIRM Histidine kinase OS=Clostridiales bacterium KLE1615 OX=1715004 GN=HMPREF2738_02903 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.04FRR4 pKa = 11.84KK5 pKa = 9.72ASSLFLAGAMCLSTFGGAMSVFADD29 pKa = 3.78EE30 pKa = 4.58EE31 pKa = 4.64TSSEE35 pKa = 3.98AAEE38 pKa = 4.62AEE40 pKa = 4.41TVDD43 pKa = 4.69YY44 pKa = 11.01SAEE47 pKa = 4.06DD48 pKa = 4.9PITATITVWGPAEE61 pKa = 4.29DD62 pKa = 3.48QAEE65 pKa = 4.49EE66 pKa = 4.07YY67 pKa = 11.25GEE69 pKa = 4.04WLQKK73 pKa = 10.28RR74 pKa = 11.84CEE76 pKa = 4.26AFNEE80 pKa = 4.1LHH82 pKa = 6.64VNWDD86 pKa = 3.33LTFEE90 pKa = 4.33YY91 pKa = 8.26GTCSEE96 pKa = 4.17GDD98 pKa = 3.2AGKK101 pKa = 8.8TISQDD106 pKa = 3.25PSGSADD112 pKa = 2.87VYY114 pKa = 10.87MFANDD119 pKa = 3.6QLQTLIDD126 pKa = 3.7AGAIAEE132 pKa = 4.45LGGKK136 pKa = 6.92TVDD139 pKa = 4.03YY140 pKa = 11.03IKK142 pKa = 10.18DD143 pKa = 3.71TNSEE147 pKa = 4.42AIVDD151 pKa = 4.17SVTVDD156 pKa = 3.22DD157 pKa = 4.82CVYY160 pKa = 10.83GVPFTTNTWYY170 pKa = 9.56MFYY173 pKa = 10.82DD174 pKa = 3.34KK175 pKa = 10.74SVYY178 pKa = 10.47DD179 pKa = 4.24DD180 pKa = 4.57EE181 pKa = 5.44DD182 pKa = 3.96VKK184 pKa = 11.61SLDD187 pKa = 3.34TMLEE191 pKa = 3.87KK192 pKa = 10.98GKK194 pKa = 10.98VSFPLTNSWYY204 pKa = 9.39IASFYY209 pKa = 10.59VGNGCTLFGEE219 pKa = 4.79DD220 pKa = 4.21GTDD223 pKa = 3.24EE224 pKa = 3.91EE225 pKa = 5.41AGIDD229 pKa = 3.69FAGEE233 pKa = 3.39KK234 pKa = 9.87GAAVTKK240 pKa = 10.49YY241 pKa = 10.42LVNLVGNKK249 pKa = 9.84NFVNDD254 pKa = 3.54EE255 pKa = 4.38SGVGVSGMCDD265 pKa = 3.09GSINAMFSGSWDD277 pKa = 3.49YY278 pKa = 11.55TKK280 pKa = 10.79LSEE283 pKa = 5.42AMGDD287 pKa = 3.25NLGIVKK293 pKa = 10.51LPTYY297 pKa = 10.42NLDD300 pKa = 3.45GEE302 pKa = 4.7EE303 pKa = 3.91IQMEE307 pKa = 4.4SFAGSKK313 pKa = 10.57AIGVNPNCEE322 pKa = 4.07YY323 pKa = 10.39PQVAVALALFLGNEE337 pKa = 4.62EE338 pKa = 4.36SQQMHH343 pKa = 6.47YY344 pKa = 10.18DD345 pKa = 3.02ARR347 pKa = 11.84SIVPCNTEE355 pKa = 3.79LLEE358 pKa = 4.35EE359 pKa = 4.2EE360 pKa = 5.21NIQKK364 pKa = 10.68DD365 pKa = 4.08EE366 pKa = 4.13LVIAQNEE373 pKa = 4.62TFDD376 pKa = 4.01EE377 pKa = 4.49TSILQPFVSAMNNYY391 pKa = 6.51WTPAEE396 pKa = 4.19NFGKK400 pKa = 10.48SIVNGEE406 pKa = 4.11VTLDD410 pKa = 3.42NAEE413 pKa = 3.98EE414 pKa = 4.15MTDD417 pKa = 3.5KK418 pKa = 11.22LSDD421 pKa = 3.53SMNQSIVDD429 pKa = 3.48

Molecular weight: 46.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A176U712|A0A176U712_9FIRM Beta-glucosidase OS=Clostridiales bacterium KLE1615 OX=1715004 GN=HMPREF2738_01404 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.3KK9 pKa = 8.13RR10 pKa = 11.84SHH12 pKa = 6.3AKK14 pKa = 8.8VHH16 pKa = 5.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3669

0

3669

1184311

27

3472

322.8

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.565 ± 0.042

1.612 ± 0.017

5.674 ± 0.032

7.438 ± 0.049

4.183 ± 0.026

6.637 ± 0.037

1.821 ± 0.017

7.033 ± 0.04

6.735 ± 0.035

8.883 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.135 ± 0.023

4.38 ± 0.027

3.288 ± 0.025

3.615 ± 0.027

4.292 ± 0.033

6.062 ± 0.037

5.575 ± 0.04

6.624 ± 0.033

1.146 ± 0.017

4.304 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski