Chryseolinea serpens
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6790 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5X1C5|A0A1M5X1C5_9BACT Iron-sulfur cluster assembly protein OS=Chryseolinea serpens OX=947013 GN=SAMN04488109_6214 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.18 KK3 pKa = 10.18 LLSIFSLSLLLLIASCRR20 pKa = 11.84 DD21 pKa = 3.18 EE22 pKa = 5.43 SYY24 pKa = 11.63 GPTTYY29 pKa = 10.49 EE30 pKa = 3.85 YY31 pKa = 10.49 ATIDD35 pKa = 3.28 TSDD38 pKa = 3.36 IPEE41 pKa = 4.45 SVTEE45 pKa = 4.79 DD46 pKa = 2.98 SDD48 pKa = 3.81 EE49 pKa = 4.33 TVTIPVLYY57 pKa = 10.68 GGTLSNATAFTINYY71 pKa = 8.54 KK72 pKa = 10.04 IEE74 pKa = 4.33 GGTYY78 pKa = 8.08 GADD81 pKa = 3.25 YY82 pKa = 10.82 VVVGGSSATGSVSIPAGTTGDD103 pKa = 3.56 KK104 pKa = 10.9 AIGLIEE110 pKa = 4.18 IVPVADD116 pKa = 4.42 LKK118 pKa = 11.23 KK119 pKa = 10.1 EE120 pKa = 4.1 PNVEE124 pKa = 3.93 LTITLVDD131 pKa = 3.99 ASNGLSLGFPGAKK144 pKa = 9.62 SYY146 pKa = 9.9 TFTVEE151 pKa = 6.27 DD152 pKa = 4.47 DD153 pKa = 3.68 DD154 pKa = 5.42 CDD156 pKa = 3.78 YY157 pKa = 11.93 VEE159 pKa = 5.41 ANFIGTAAGRR169 pKa = 11.84 EE170 pKa = 4.21 FYY172 pKa = 10.69 DD173 pKa = 5.18 DD174 pKa = 3.55 GSTYY178 pKa = 9.04 PTDD181 pKa = 4.2 PDD183 pKa = 3.59 APGYY187 pKa = 10.51 DD188 pKa = 3.08 VGFTSTGTNEE198 pKa = 3.86 FEE200 pKa = 4.65 MDD202 pKa = 3.87 NFWGSSYY209 pKa = 10.58 HH210 pKa = 6.76 VSFTIDD216 pKa = 3.01 PTTFVVTVPGQDD228 pKa = 3.14 IEE230 pKa = 5.64 DD231 pKa = 4.09 GFHH234 pKa = 6.78 VDD236 pKa = 3.06 GTGTVSTCGKK246 pKa = 9.01 TITITMNMDD255 pKa = 3.21 GPGYY259 pKa = 9.34 TGTFKK264 pKa = 10.86 NVYY267 pKa = 8.09 TFPEE271 pKa = 3.97
Molecular weight: 28.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.439
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 0.362
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1M5LLE9|A0A1M5LLE9_9BACT Trehalose utilisation OS=Chryseolinea serpens OX=947013 GN=SAMN04488109_1282 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 LLFLHH7 pKa = 7.09 IYY9 pKa = 9.69 KK10 pKa = 10.27 IIVQRR15 pKa = 11.84 ISSFHH20 pKa = 5.46 SRR22 pKa = 11.84 KK23 pKa = 9.23 FLNSVGVLYY32 pKa = 10.69 HH33 pKa = 6.05 NTGCQPGVCEE43 pKa = 3.99 PTIHH47 pKa = 6.84 LRR49 pKa = 11.84 HH50 pKa = 6.44 APTKK54 pKa = 8.95 IEE56 pKa = 3.71 ARR58 pKa = 11.84 HH59 pKa = 4.07 VHH61 pKa = 5.67 GAHH64 pKa = 5.97 RR65 pKa = 11.84 TALGCSVPLRR75 pKa = 11.84 AKK77 pKa = 9.7 PFEE80 pKa = 4.42 ARR82 pKa = 11.84 SHH84 pKa = 6.14 FPVALLLFSSLPRR97 pKa = 11.84 VKK99 pKa = 9.89 TRR101 pKa = 11.84 VMMGNSYY108 pKa = 10.04 GVRR111 pKa = 11.84 EE112 pKa = 4.2 SGGNGVPKK120 pKa = 10.41 KK121 pKa = 9.98 KK122 pKa = 10.02 QRR124 pKa = 11.84 LALFLHH130 pKa = 5.76 GHH132 pKa = 6.8 KK133 pKa = 10.28 IFLFAIAHH141 pKa = 4.79 MTAIAFGQFAKK152 pKa = 10.46 RR153 pKa = 11.84 CPCWNMRR160 pKa = 11.84 DD161 pKa = 3.61 LVAVFLVVDD170 pKa = 4.72 GQAVAALIDD179 pKa = 4.0 GVGMAIYY186 pKa = 9.45 RR187 pKa = 11.84 WSVGG191 pKa = 3.2
Molecular weight: 21.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.809
IPC_protein 10.613
Toseland 10.862
ProMoST 10.555
Dawson 10.935
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.199
Grimsley 10.979
Solomon 11.067
Lehninger 11.038
Nozaki 10.862
DTASelect 10.643
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.877
Patrickios 10.921
IPC_peptide 11.082
IPC2_peptide 9.853
IPC2.peptide.svr19 8.495
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6790
0
6790
2474512
39
4239
364.4
40.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.886 ± 0.029
0.762 ± 0.009
5.463 ± 0.023
5.673 ± 0.03
5.013 ± 0.02
7.03 ± 0.031
2.057 ± 0.014
6.297 ± 0.026
6.366 ± 0.032
9.612 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.316 ± 0.015
4.779 ± 0.029
4.157 ± 0.022
3.662 ± 0.015
4.489 ± 0.02
6.25 ± 0.024
6.097 ± 0.039
6.925 ± 0.023
1.294 ± 0.013
3.875 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here