Siccibacter turicensis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3928 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8VPG5|A0A2P8VPG5_9ENTR Glutamate/aspartate ABC transporter permease GltK OS=Siccibacter turicensis OX=357233 GN=C7G83_01510 PE=3 SV=1
MM1 pKa = 7.83 AKK3 pKa = 9.97 PEE5 pKa = 4.03 HH6 pKa = 7.2 LEE8 pKa = 3.88 EE9 pKa = 4.08 QRR11 pKa = 11.84 EE12 pKa = 4.15 EE13 pKa = 3.72 TRR15 pKa = 11.84 LIIEE19 pKa = 4.54 EE20 pKa = 4.2 LLEE23 pKa = 5.25 DD24 pKa = 4.98 GSDD27 pKa = 3.46 PDD29 pKa = 3.46 ALYY32 pKa = 9.93 TIEE35 pKa = 4.48 HH36 pKa = 6.82 HH37 pKa = 7.16 LSADD41 pKa = 3.64 DD42 pKa = 4.93 FEE44 pKa = 4.77 TLEE47 pKa = 4.23 KK48 pKa = 10.72 AAVEE52 pKa = 4.04 AFKK55 pKa = 10.9 LGYY58 pKa = 10.03 EE59 pKa = 4.19 VTDD62 pKa = 3.9 PEE64 pKa = 4.16 EE65 pKa = 5.49 LEE67 pKa = 4.32 VEE69 pKa = 4.23 EE70 pKa = 5.46 GDD72 pKa = 4.38 TVICCDD78 pKa = 3.75 VVSEE82 pKa = 4.35 CALNAEE88 pKa = 5.01 LIDD91 pKa = 4.02 AQVEE95 pKa = 4.22 QLLNLAEE102 pKa = 4.28 KK103 pKa = 10.19 MGVEE107 pKa = 3.93 YY108 pKa = 10.61 DD109 pKa = 2.91 GWGTYY114 pKa = 10.16 FEE116 pKa = 5.36 DD117 pKa = 4.27 PNGEE121 pKa = 4.0 EE122 pKa = 4.63 GEE124 pKa = 4.24 DD125 pKa = 3.52 DD126 pKa = 3.85 EE127 pKa = 6.3 EE128 pKa = 4.74 YY129 pKa = 11.4 VDD131 pKa = 6.29 DD132 pKa = 4.65 EE133 pKa = 5.93 DD134 pKa = 6.46 DD135 pKa = 4.98 GIRR138 pKa = 11.84 HH139 pKa = 6.07
Molecular weight: 15.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.516
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.516
EMBOSS 3.528
Sillero 3.783
Patrickios 1.799
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A0A2P8VIJ5|A0A2P8VIJ5_9ENTR Phosphate transport system permease protein PstA OS=Siccibacter turicensis OX=357233 GN=C7G83_13960 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LTVSKK46 pKa = 11.03
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3928
0
3928
1227987
16
4075
312.6
34.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.39 ± 0.042
1.034 ± 0.015
5.372 ± 0.032
5.511 ± 0.039
3.737 ± 0.029
7.623 ± 0.053
2.288 ± 0.02
5.408 ± 0.033
3.814 ± 0.032
10.867 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.704 ± 0.023
3.548 ± 0.034
4.632 ± 0.032
4.411 ± 0.029
6.004 ± 0.044
5.719 ± 0.029
5.505 ± 0.04
7.23 ± 0.037
1.533 ± 0.02
2.671 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here