Fusarium kuroshium
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16484 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M2SA90|A0A3M2SA90_9HYPO Uncharacterized protein OS=Fusarium kuroshium OX=2010991 GN=CDV36_005831 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 VTEE5 pKa = 4.85 LLLLSLAAASPVACADD21 pKa = 3.43 TATPDD26 pKa = 4.39 GPFQLYY32 pKa = 10.38 AYY34 pKa = 9.58 GEE36 pKa = 4.57 GVGGLVLFSDD46 pKa = 4.37 GDD48 pKa = 3.77 LVYY51 pKa = 10.88 AGEE54 pKa = 4.34 PSLLDD59 pKa = 3.38 SDD61 pKa = 3.82 QAAPIIAVSNDD72 pKa = 3.67 GQWSCSPNTTALANDD87 pKa = 4.36 AQPTWSNLTFAIPSAAATSHH107 pKa = 7.11 DD108 pKa = 3.82 VQLVNTTRR116 pKa = 11.84 NGTSSLITDD125 pKa = 4.19 GFSFYY130 pKa = 11.16 GAIAFASIDD139 pKa = 3.62 GGLVSLWQGIPSDD152 pKa = 3.9 VEE154 pKa = 4.09 GVYY157 pKa = 10.38 SLKK160 pKa = 10.79 WNDD163 pKa = 3.42 TEE165 pKa = 4.27 EE166 pKa = 4.71 GNGAVIFTVKK176 pKa = 8.97 ATKK179 pKa = 9.94 PSSTT183 pKa = 3.7
Molecular weight: 18.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.478
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.821
Patrickios 0.846
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A3M2RA98|A0A3M2RA98_9HYPO Chitinase OS=Fusarium kuroshium OX=2010991 GN=CDV36_015524 PE=3 SV=1
MM1 pKa = 7.86 PLTRR5 pKa = 11.84 THH7 pKa = 6.67 RR8 pKa = 11.84 HH9 pKa = 3.83 TTPRR13 pKa = 11.84 RR14 pKa = 11.84 SIFSTRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APAHH26 pKa = 5.0 SHH28 pKa = 5.34 HH29 pKa = 6.37 TTVTTTTTTRR39 pKa = 11.84 KK40 pKa = 8.96 PRR42 pKa = 11.84 RR43 pKa = 11.84 GLLGGGHH50 pKa = 5.92 RR51 pKa = 11.84 THH53 pKa = 7.13 ATPVHH58 pKa = 5.39 HH59 pKa = 5.5 QQRR62 pKa = 11.84 RR63 pKa = 11.84 PSMKK67 pKa = 10.24 DD68 pKa = 2.95 KK69 pKa = 11.41 VSGALLKK76 pKa = 11.04 LKK78 pKa = 10.68 GSLTRR83 pKa = 11.84 RR84 pKa = 11.84 PGVKK88 pKa = 9.89 AAGTRR93 pKa = 11.84 RR94 pKa = 11.84 MRR96 pKa = 11.84 GTDD99 pKa = 2.74 GRR101 pKa = 11.84 GSHH104 pKa = 5.81 RR105 pKa = 11.84 RR106 pKa = 11.84 ARR108 pKa = 11.84 HH109 pKa = 4.06 FF110 pKa = 3.77
Molecular weight: 12.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.477
IPC2_protein 11.038
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.427
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.135
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.131
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16484
0
16484
7632786
50
8501
463.0
51.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.429 ± 0.019
1.343 ± 0.008
5.892 ± 0.015
6.276 ± 0.024
3.833 ± 0.012
6.888 ± 0.02
2.381 ± 0.009
5.01 ± 0.015
4.824 ± 0.017
8.99 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.008
3.583 ± 0.01
5.961 ± 0.022
3.926 ± 0.014
5.954 ± 0.017
7.926 ± 0.019
5.96 ± 0.019
6.206 ± 0.014
1.617 ± 0.008
2.758 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here