Aliidiomarina haloalkalitolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Aliidiomarina

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A432VPH2|A0A432VPH2_9GAMM Uncharacterized protein OS=Aliidiomarina haloalkalitolerans OX=859059 GN=CWE06_12375 PE=4 SV=1
MM1 pKa = 7.05NRR3 pKa = 11.84VMQMTAAVLAVGTLAACDD21 pKa = 3.93SSSSDD26 pKa = 3.47DD27 pKa = 3.41NTAIFSLAVSDD38 pKa = 4.69APVDD42 pKa = 3.62SAEE45 pKa = 4.03RR46 pKa = 11.84VLVCFSSVEE55 pKa = 3.89LVGNGEE61 pKa = 3.82GSQRR65 pKa = 11.84FEE67 pKa = 3.96VGGDD71 pKa = 3.32NGAVPANDD79 pKa = 3.93LCLDD83 pKa = 3.96DD84 pKa = 5.19SGNPIPNTRR93 pKa = 11.84GVDD96 pKa = 3.65LLTLQGANAEE106 pKa = 4.31SLVVGAEE113 pKa = 4.39VPAGTYY119 pKa = 10.27GQMRR123 pKa = 11.84LDD125 pKa = 3.28IANGSEE131 pKa = 4.01IEE133 pKa = 4.25LTDD136 pKa = 3.6GTIHH140 pKa = 7.83DD141 pKa = 4.83LRR143 pKa = 11.84VPSNQLRR150 pKa = 11.84LDD152 pKa = 4.07GPTLTANQTFNYY164 pKa = 9.01TLEE167 pKa = 4.16FDD169 pKa = 3.41LRR171 pKa = 11.84KK172 pKa = 9.98AVVAPPGLPHH182 pKa = 6.79YY183 pKa = 9.82LLKK186 pKa = 10.7PRR188 pKa = 11.84GLRR191 pKa = 11.84LVDD194 pKa = 3.42NAEE197 pKa = 3.94VGHH200 pKa = 7.4IEE202 pKa = 4.15GQVAEE207 pKa = 4.29TLLLEE212 pKa = 4.42NEE214 pKa = 4.54CAVAPEE220 pKa = 4.47DD221 pKa = 3.52LTTPVAAVYY230 pKa = 9.69LYY232 pKa = 10.57NGHH235 pKa = 7.06DD236 pKa = 3.46VDD238 pKa = 5.09FADD241 pKa = 5.04MSDD244 pKa = 3.79NSDD247 pKa = 4.5DD248 pKa = 3.89EE249 pKa = 4.6NGPYY253 pKa = 10.71ASTAVFFDD261 pKa = 3.97GAAAYY266 pKa = 10.03NFAIGFIQAGQYY278 pKa = 9.05TIAVTCDD285 pKa = 3.19TEE287 pKa = 4.82DD288 pKa = 3.88DD289 pKa = 4.19PEE291 pKa = 5.15EE292 pKa = 3.95EE293 pKa = 4.22TEE295 pKa = 4.53LNFFHH300 pKa = 7.17SEE302 pKa = 4.1NIDD305 pKa = 3.25IEE307 pKa = 4.32QGVTLEE313 pKa = 4.26IVVGADD319 pKa = 3.04SS320 pKa = 3.57

Molecular weight: 33.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A432VS88|A0A432VS88_9GAMM Cytochrome c4 OS=Aliidiomarina haloalkalitolerans OX=859059 GN=CWE06_09180 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2391

0

2391

807456

36

1614

337.7

37.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.104 ± 0.058

0.837 ± 0.015

5.371 ± 0.04

6.38 ± 0.044

4.053 ± 0.033

7.066 ± 0.048

2.376 ± 0.025

5.591 ± 0.034

3.688 ± 0.046

10.615 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.448 ± 0.021

3.605 ± 0.031

4.277 ± 0.028

5.142 ± 0.042

6.017 ± 0.039

5.746 ± 0.036

5.189 ± 0.028

7.29 ± 0.04

1.36 ± 0.02

2.845 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski