Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema; Actinosynnema mirum

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6912 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6WF78|C6WF78_ACTMD Endoribonuclease L-PSP OS=Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971) OX=446462 GN=Amir_0241 PE=4 SV=1
MM1 pKa = 7.26IAVPAGGEE9 pKa = 4.2VEE11 pKa = 5.33LDD13 pKa = 3.52LLLEE17 pKa = 4.33SVVEE21 pKa = 4.38GVLVTGTASTTVEE34 pKa = 4.63GEE36 pKa = 4.26CSRR39 pKa = 11.84CLDD42 pKa = 4.12PLSSDD47 pKa = 3.62VQVGLTEE54 pKa = 4.82LYY56 pKa = 10.22AYY58 pKa = 8.87PDD60 pKa = 3.53SATDD64 pKa = 3.3EE65 pKa = 4.38TTEE68 pKa = 3.83EE69 pKa = 4.5DD70 pKa = 3.88EE71 pKa = 4.45VSRR74 pKa = 11.84VHH76 pKa = 7.76DD77 pKa = 4.39DD78 pKa = 5.01LIDD81 pKa = 4.0LEE83 pKa = 4.5PVVRR87 pKa = 11.84DD88 pKa = 3.74ALVLALPQVPLCSPDD103 pKa = 3.96CLGLCVDD110 pKa = 4.54CGGRR114 pKa = 11.84LADD117 pKa = 4.86LGPDD121 pKa = 3.66HH122 pKa = 6.67GHH124 pKa = 5.46EE125 pKa = 4.58TIDD128 pKa = 3.81PRR130 pKa = 11.84WAALQVRR137 pKa = 11.84FDD139 pKa = 3.97GNRR142 pKa = 11.84DD143 pKa = 3.47NPEE146 pKa = 3.83EE147 pKa = 3.97NN148 pKa = 3.38

Molecular weight: 15.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6W7Z7|C6W7Z7_ACTMD N-acetyltransferase domain-containing protein OS=Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971) OX=446462 GN=Amir_3104 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILSARR37 pKa = 11.84RR38 pKa = 11.84TKK40 pKa = 10.47GRR42 pKa = 11.84KK43 pKa = 8.22QLSAA47 pKa = 3.9

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6912

0

6912

2388939

30

9529

345.6

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.178 ± 0.053

0.73 ± 0.008

5.971 ± 0.023

5.794 ± 0.029

2.624 ± 0.017

9.84 ± 0.038

2.197 ± 0.015

2.448 ± 0.02

1.742 ± 0.021

10.715 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.413 ± 0.013

1.676 ± 0.021

6.217 ± 0.035

2.535 ± 0.02

8.266 ± 0.038

5.034 ± 0.025

5.747 ± 0.037

9.677 ± 0.035

1.504 ± 0.011

1.692 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski