Amino acid dipepetide frequency for Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.58AlaAla: 21.58 ± 0.166	1.059AlaCys: 1.059 ± 0.021	8.765AlaAsp: 8.765 ± 0.069	9.762AlaGlu: 9.762 ± 0.081	3.491AlaPhe: 3.491 ± 0.045	13.816AlaGly: 13.816 ± 0.118	3.104AlaHis: 3.104 ± 0.042	3.264AlaIle: 3.264 ± 0.036	2.515AlaLys: 2.515 ± 0.055	16.859AlaLeu: 16.859 ± 0.141	2.051AlaMet: 2.051 ± 0.031	2.166AlaAsn: 2.166 ± 0.04	7.585AlaPro: 7.585 ± 0.091	3.709AlaGln: 3.709 ± 0.048	11.017AlaArg: 11.017 ± 0.104	6.109AlaSer: 6.109 ± 0.053	7.254AlaThr: 7.254 ± 0.091	13.606AlaVal: 13.606 ± 0.091	1.93AlaTrp: 1.93 ± 0.029	2.134AlaTyr: 2.134 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.198CysAla: 1.198 ± 0.024	0.084CysCys: 0.084 ± 0.008	0.436CysAsp: 0.436 ± 0.015	0.376CysGlu: 0.376 ± 0.013	0.183CysPhe: 0.183 ± 0.009	0.892CysGly: 0.892 ± 0.022	0.166CysHis: 0.166 ± 0.008	0.081CysIle: 0.081 ± 0.007	0.095CysLys: 0.095 ± 0.007	0.733CysLeu: 0.733 ± 0.02	0.085CysMet: 0.085 ± 0.006	0.102CysAsn: 0.102 ± 0.007	0.449CysPro: 0.449 ± 0.014	0.143CysGln: 0.143 ± 0.008	0.545CysArg: 0.545 ± 0.016	0.437CysSer: 0.437 ± 0.015	0.411CysThr: 0.411 ± 0.019	0.617CysVal: 0.617 ± 0.016	0.112CysTrp: 0.112 ± 0.007	0.154CysTyr: 0.154 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.872AspAla: 7.872 ± 0.087	0.395AspCys: 0.395 ± 0.014	3.436AspAsp: 3.436 ± 0.05	3.965AspGlu: 3.965 ± 0.055	1.499AspPhe: 1.499 ± 0.028	6.539AspGly: 6.539 ± 0.074	1.445AspHis: 1.445 ± 0.029	1.072AspIle: 1.072 ± 0.024	0.879AspLys: 0.879 ± 0.021	6.951AspLeu: 6.951 ± 0.065	0.618AspMet: 0.618 ± 0.017	0.889AspAsn: 0.889 ± 0.021	4.708AspPro: 4.708 ± 0.054	1.524AspGln: 1.524 ± 0.028	5.217AspArg: 5.217 ± 0.065	2.422AspSer: 2.422 ± 0.042	2.499AspThr: 2.499 ± 0.039	5.648AspVal: 5.648 ± 0.048	0.985AspTrp: 0.985 ± 0.021	1.143AspTyr: 1.143 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.235GluAla: 7.235 ± 0.073	0.357GluCys: 0.357 ± 0.013	2.778GluAsp: 2.778 ± 0.039	3.044GluGlu: 3.044 ± 0.052	1.605GluPhe: 1.605 ± 0.025	4.269GluGly: 4.269 ± 0.051	1.897GluHis: 1.897 ± 0.031	1.743GluIle: 1.743 ± 0.029	1.016GluLys: 1.016 ± 0.025	7.864GluLeu: 7.864 ± 0.08	0.689GluMet: 0.689 ± 0.018	0.914GluAsn: 0.914 ± 0.025	3.652GluPro: 3.652 ± 0.054	2.29GluGln: 2.29 ± 0.033	6.169GluArg: 6.169 ± 0.07	2.515GluSer: 2.515 ± 0.036	2.296GluThr: 2.296 ± 0.034	5.96GluVal: 5.96 ± 0.059	0.792GluTrp: 0.792 ± 0.019	0.857GluTyr: 0.857 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.847PheAla: 3.847 ± 0.044	0.237PheCys: 0.237 ± 0.011	2.023PheAsp: 2.023 ± 0.032	1.318PheGlu: 1.318 ± 0.03	0.753PhePhe: 0.753 ± 0.025	3.034PheGly: 3.034 ± 0.041	0.625PheHis: 0.625 ± 0.019	0.494PheIle: 0.494 ± 0.014	0.398PheLys: 0.398 ± 0.015	2.449PheLeu: 2.449 ± 0.037	0.292PheMet: 0.292 ± 0.012	0.465PheAsn: 0.465 ± 0.015	1.435PhePro: 1.435 ± 0.027	0.677PheGln: 0.677 ± 0.019	1.775PheArg: 1.775 ± 0.03	1.442PheSer: 1.442 ± 0.025	2.07PheThr: 2.07 ± 0.03	2.037PheVal: 2.037 ± 0.032	0.368PheTrp: 0.368 ± 0.011	0.504PheTyr: 0.504 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	12.905GlyAla: 12.905 ± 0.092	0.798GlyCys: 0.798 ± 0.022	5.444GlyAsp: 5.444 ± 0.056	5.748GlyGlu: 5.748 ± 0.058	3.016GlyPhe: 3.016 ± 0.039	10.113GlyGly: 10.113 ± 0.119	2.18GlyHis: 2.18 ± 0.032	2.712GlyIle: 2.712 ± 0.043	2.152GlyLys: 2.152 ± 0.04	9.703GlyLeu: 9.703 ± 0.072	1.9GlyMet: 1.9 ± 0.031	1.681GlyAsn: 1.681 ± 0.041	5.166GlyPro: 5.166 ± 0.067	2.795GlyGln: 2.795 ± 0.043	7.73GlyArg: 7.73 ± 0.073	5.54GlySer: 5.54 ± 0.074	5.373GlyThr: 5.373 ± 0.068	9.523GlyVal: 9.523 ± 0.1	1.764GlyTrp: 1.764 ± 0.027	2.152GlyTyr: 2.152 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.679HisAla: 2.679 ± 0.039	0.184HisCys: 0.184 ± 0.01	1.384HisAsp: 1.384 ± 0.024	1.251HisGlu: 1.251 ± 0.022	0.57HisPhe: 0.57 ± 0.018	2.229HisGly: 2.229 ± 0.032	0.687HisHis: 0.687 ± 0.02	0.381HisIle: 0.381 ± 0.013	0.26HisLys: 0.26 ± 0.011	2.503HisLeu: 2.503 ± 0.039	0.211HisMet: 0.211 ± 0.01	0.345HisAsn: 0.345 ± 0.013	1.819HisPro: 1.819 ± 0.029	0.547HisGln: 0.547 ± 0.019	2.222HisArg: 2.222 ± 0.035	0.919HisSer: 0.919 ± 0.019	0.993HisThr: 0.993 ± 0.021	2.021HisVal: 2.021 ± 0.029	0.351HisTrp: 0.351 ± 0.012	0.412HisTyr: 0.412 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.237IleAla: 4.237 ± 0.048	0.169IleCys: 0.169 ± 0.008	1.543IleAsp: 1.543 ± 0.028	1.351IleGlu: 1.351 ± 0.027	0.457IlePhe: 0.457 ± 0.017	3.138IleGly: 3.138 ± 0.048	0.357IleHis: 0.357 ± 0.013	0.581IleIle: 0.581 ± 0.019	0.545IleLys: 0.545 ± 0.017	1.376IleLeu: 1.376 ± 0.026	0.318IleMet: 0.318 ± 0.013	0.529IleAsn: 0.529 ± 0.016	1.42IlePro: 1.42 ± 0.025	0.494IleGln: 0.494 ± 0.017	1.649IleArg: 1.649 ± 0.027	1.452IleSer: 1.452 ± 0.027	2.221IleThr: 2.221 ± 0.042	2.044IleVal: 2.044 ± 0.036	0.244IleTrp: 0.244 ± 0.011	0.351IleTyr: 0.351 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	2.391LysAla: 2.391 ± 0.052	0.1LysCys: 0.1 ± 0.008	0.878LysAsp: 0.878 ± 0.022	0.8LysGlu: 0.8 ± 0.021	0.421LysPhe: 0.421 ± 0.016	1.371LysGly: 1.371 ± 0.032	0.416LysHis: 0.416 ± 0.014	0.621LysIle: 0.621 ± 0.018	0.48LysLys: 0.48 ± 0.021	1.824LysLeu: 1.824 ± 0.031	0.281LysMet: 0.281 ± 0.012	0.343LysAsn: 0.343 ± 0.015	1.225LysPro: 1.225 ± 0.029	0.591LysGln: 0.591 ± 0.019	1.402LysArg: 1.402 ± 0.03	1.014LysSer: 1.014 ± 0.023	0.941LysThr: 0.941 ± 0.025	1.772LysVal: 1.772 ± 0.034	0.228LysTrp: 0.228 ± 0.01	0.315LysTyr: 0.315 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	16.5LeuAla: 16.5 ± 0.143	0.785LeuCys: 0.785 ± 0.017	7.01LeuAsp: 7.01 ± 0.059	5.013LeuGlu: 5.013 ± 0.055	2.543LeuPhe: 2.543 ± 0.044	10.106LeuGly: 10.106 ± 0.082	2.224LeuHis: 2.224 ± 0.035	2.493LeuIle: 2.493 ± 0.038	1.576LeuLys: 1.576 ± 0.032	12.162LeuLeu: 12.162 ± 0.118	1.259LeuMet: 1.259 ± 0.023	1.572LeuAsn: 1.572 ± 0.031	6.879LeuPro: 6.879 ± 0.064	1.769LeuGln: 1.769 ± 0.029	9.529LeuArg: 9.529 ± 0.098	5.414LeuSer: 5.414 ± 0.057	6.241LeuThr: 6.241 ± 0.062	11.352LeuVal: 11.352 ± 0.089	1.305LeuTrp: 1.305 ± 0.027	1.422LeuTyr: 1.422 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	1.921MetAla: 1.921 ± 0.027	0.098MetCys: 0.098 ± 0.007	0.663MetAsp: 0.663 ± 0.015	0.512MetGlu: 0.512 ± 0.015	0.359MetPhe: 0.359 ± 0.014	1.141MetGly: 1.141 ± 0.024	0.272MetHis: 0.272 ± 0.012	0.503MetIle: 0.503 ± 0.014	0.273MetLys: 0.273 ± 0.012	1.34MetLeu: 1.34 ± 0.03	0.209MetMet: 0.209 ± 0.01	0.312MetAsn: 0.312 ± 0.012	0.929MetPro: 0.929 ± 0.022	0.345MetGln: 0.345 ± 0.012	1.296MetArg: 1.296 ± 0.024	1.134MetSer: 1.134 ± 0.024	1.28MetThr: 1.28 ± 0.027	1.186MetVal: 1.186 ± 0.023	0.158MetTrp: 0.158 ± 0.008	0.201MetTyr: 0.201 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.253AsnAla: 2.253 ± 0.038	0.144AsnCys: 0.144 ± 0.008	0.818AsnAsp: 0.818 ± 0.025	0.73AsnGlu: 0.73 ± 0.018	0.431AsnPhe: 0.431 ± 0.014	1.831AsnGly: 1.831 ± 0.038	0.37AsnHis: 0.37 ± 0.013	0.465AsnIle: 0.465 ± 0.017	0.316AsnLys: 0.316 ± 0.012	1.587AsnLeu: 1.587 ± 0.026	0.202AsnMet: 0.202 ± 0.011	0.395AsnAsn: 0.395 ± 0.017	1.468AsnPro: 1.468 ± 0.033	0.53AsnGln: 0.53 ± 0.02	1.339AsnArg: 1.339 ± 0.025	0.908AsnSer: 0.908 ± 0.024	1.056AsnThr: 1.056 ± 0.028	1.256AsnVal: 1.256 ± 0.027	0.27AsnTrp: 0.27 ± 0.012	0.396AsnTyr: 0.396 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.562ProAla: 8.562 ± 0.104	0.339ProCys: 0.339 ± 0.014	4.794ProAsp: 4.794 ± 0.061	4.767ProGlu: 4.767 ± 0.062	1.493ProPhe: 1.493 ± 0.021	6.751ProGly: 6.751 ± 0.068	1.269ProHis: 1.269 ± 0.024	1.228ProIle: 1.228 ± 0.023	1.029ProLys: 1.029 ± 0.028	5.61ProLeu: 5.61 ± 0.05	0.842ProMet: 0.842 ± 0.021	1.044ProAsn: 1.044 ± 0.025	3.946ProPro: 3.946 ± 0.076	1.628ProGln: 1.628 ± 0.039	3.99ProArg: 3.99 ± 0.051	3.188ProSer: 3.188 ± 0.055	3.507ProThr: 3.507 ± 0.055	6.324ProVal: 6.324 ± 0.063	0.973ProTrp: 0.973 ± 0.022	0.885ProTyr: 0.885 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.636GlnAla: 3.636 ± 0.052	0.151GlnCys: 0.151 ± 0.009	1.246GlnAsp: 1.246 ± 0.028	1.319GlnGlu: 1.319 ± 0.026	0.655GlnPhe: 0.655 ± 0.016	2.111GlnGly: 2.111 ± 0.04	0.601GlnHis: 0.601 ± 0.013	0.718GlnIle: 0.718 ± 0.022	0.426GlnLys: 0.426 ± 0.019	2.661GlnLeu: 2.661 ± 0.04	0.306GlnMet: 0.306 ± 0.011	0.462GlnAsn: 0.462 ± 0.018	1.697GlnPro: 1.697 ± 0.043	1.087GlnGln: 1.087 ± 0.034	2.492GlnArg: 2.492 ± 0.039	1.139GlnSer: 1.139 ± 0.026	1.062GlnThr: 1.062 ± 0.025	2.77GlnVal: 2.77 ± 0.034	0.4GlnTrp: 0.4 ± 0.012	0.408GlnTyr: 0.408 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	11.735ArgAla: 11.735 ± 0.114	0.615ArgCys: 0.615 ± 0.017	4.525ArgAsp: 4.525 ± 0.05	5.214ArgGlu: 5.214 ± 0.058	2.523ArgPhe: 2.523 ± 0.032	6.792ArgGly: 6.792 ± 0.07	1.854ArgHis: 1.854 ± 0.027	2.359ArgIle: 2.359 ± 0.037	1.512ArgLys: 1.512 ± 0.03	8.543ArgLeu: 8.543 ± 0.085	1.705ArgMet: 1.705 ± 0.03	1.316ArgAsn: 1.316 ± 0.022	4.827ArgPro: 4.827 ± 0.057	1.964ArgGln: 1.964 ± 0.031	7.486ArgArg: 7.486 ± 0.086	4.065ArgSer: 4.065 ± 0.04	4.674ArgThr: 4.674 ± 0.046	7.778ArgVal: 7.778 ± 0.066	1.538ArgTrp: 1.538 ± 0.026	1.632ArgTyr: 1.632 ± 0.029	0.0ArgXaa: 0.0 ± 0.0
Ser	7.435SerAla: 7.435 ± 0.062	0.369SerCys: 0.369 ± 0.015	2.515SerAsp: 2.515 ± 0.038	2.301SerGlu: 2.301 ± 0.035	1.417SerPhe: 1.417 ± 0.026	6.574SerGly: 6.574 ± 0.076	0.842SerHis: 0.842 ± 0.018	1.239SerIle: 1.239 ± 0.027	0.833SerLys: 0.833 ± 0.02	4.692SerLeu: 4.692 ± 0.052	0.849SerMet: 0.849 ± 0.019	0.852SerAsn: 0.852 ± 0.024	3.195SerPro: 3.195 ± 0.042	1.103SerGln: 1.103 ± 0.023	3.486SerArg: 3.486 ± 0.043	2.898SerSer: 2.898 ± 0.051	3.438SerThr: 3.438 ± 0.052	4.398SerVal: 4.398 ± 0.044	0.903SerTrp: 0.903 ± 0.023	1.003SerTyr: 1.003 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.002ThrAla: 9.002 ± 0.093	0.442ThrCys: 0.442 ± 0.017	2.936ThrAsp: 2.936 ± 0.04	2.814ThrGlu: 2.814 ± 0.034	1.311ThrPhe: 1.311 ± 0.026	7.049ThrGly: 7.049 ± 0.083	0.977ThrHis: 0.977 ± 0.023	1.488ThrIle: 1.488 ± 0.032	0.98ThrLys: 0.98 ± 0.026	4.558ThrLeu: 4.558 ± 0.051	0.743ThrMet: 0.743 ± 0.019	0.969ThrAsn: 0.969 ± 0.027	4.304ThrPro: 4.304 ± 0.061	1.08ThrGln: 1.08 ± 0.025	3.992ThrArg: 3.992 ± 0.038	3.387ThrSer: 3.387 ± 0.054	4.744ThrThr: 4.744 ± 0.108	4.777ThrVal: 4.777 ± 0.063	0.936ThrTrp: 0.936 ± 0.019	0.984ThrTyr: 0.984 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	12.816ValAla: 12.816 ± 0.091	0.698ValCys: 0.698 ± 0.017	6.6ValAsp: 6.6 ± 0.057	6.007ValGlu: 6.007 ± 0.057	2.498ValPhe: 2.498 ± 0.034	7.891ValGly: 7.891 ± 0.068	1.953ValHis: 1.953 ± 0.029	2.384ValIle: 2.384 ± 0.034	1.587ValLys: 1.587 ± 0.035	11.94ValLeu: 11.94 ± 0.09	1.167ValMet: 1.167 ± 0.023	1.705ValAsn: 1.705 ± 0.031	5.688ValPro: 5.688 ± 0.063	2.04ValGln: 2.04 ± 0.034	8.095ValArg: 8.095 ± 0.078	4.448ValSer: 4.448 ± 0.052	5.497ValThr: 5.497 ± 0.065	11.286ValVal: 11.286 ± 0.1	1.15ValTrp: 1.15 ± 0.023	1.321ValTyr: 1.321 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.721TrpAla: 1.721 ± 0.028	0.139TrpCys: 0.139 ± 0.007	0.812TrpAsp: 0.812 ± 0.02	0.748TrpGlu: 0.748 ± 0.018	0.491TrpPhe: 0.491 ± 0.016	1.07TrpGly: 1.07 ± 0.022	0.368TrpHis: 0.368 ± 0.013	0.399TrpIle: 0.399 ± 0.014	0.28TrpLys: 0.28 ± 0.012	1.8TrpLeu: 1.8 ± 0.032	0.255TrpMet: 0.255 ± 0.01	0.371TrpAsn: 0.371 ± 0.013	0.827TrpPro: 0.827 ± 0.022	0.528TrpGln: 0.528 ± 0.013	1.461TrpArg: 1.461 ± 0.025	1.002TrpSer: 1.002 ± 0.021	1.007TrpThr: 1.007 ± 0.019	1.15TrpVal: 1.15 ± 0.023	0.344TrpTrp: 0.344 ± 0.014	0.27TrpTyr: 0.27 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.25TyrAla: 2.25 ± 0.031	0.14TyrCys: 0.14 ± 0.007	1.099TyrAsp: 1.099 ± 0.028	0.902TyrGlu: 0.902 ± 0.022	0.527TyrPhe: 0.527 ± 0.015	1.679TyrGly: 1.679 ± 0.033	0.361TyrHis: 0.361 ± 0.014	0.253TyrIle: 0.253 ± 0.01	0.264TyrLys: 0.264 ± 0.012	1.998TyrLeu: 1.998 ± 0.03	0.147TyrMet: 0.147 ± 0.008	0.332TyrAsn: 0.332 ± 0.013	0.951TyrPro: 0.951 ± 0.02	0.504TyrGln: 0.504 ± 0.015	1.764TyrArg: 1.764 ± 0.028	0.909TyrSer: 0.909 ± 0.021	0.905TyrThr: 0.905 ± 0.027	1.267TyrVal: 1.267 ± 0.024	0.292TyrTrp: 0.292 ± 0.012	0.378TyrTyr: 0.378 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6912 proteins (2388939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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