Vogesella sp. LIG4
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3722 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C6MT89|A0A1C6MT89_9NEIS Acyl-CoA reductase OS=Vogesella sp. LIG4 OX=1192162 GN=PSELUDRAFT_2512 PE=3 SV=1
SS1 pKa = 7.15 ASVDD5 pKa = 3.56 ADD7 pKa = 4.01 GTWHH11 pKa = 5.47 YY12 pKa = 9.31 TVSDD16 pKa = 3.86 SGTVDD21 pKa = 3.17 ALAAGEE27 pKa = 4.13 TLGDD31 pKa = 3.79 SFTVRR36 pKa = 11.84 VSDD39 pKa = 3.43 NHH41 pKa = 6.89 GGFVDD46 pKa = 3.6 QLVSVTITGTNDD58 pKa = 2.83 APLIDD63 pKa = 4.54 AANTTASGGVSEE75 pKa = 5.68 GDD77 pKa = 3.8 DD78 pKa = 3.28 NSSRR82 pKa = 11.84 STSGVIAYY90 pKa = 10.36 SDD92 pKa = 3.39 VDD94 pKa = 3.5 ASDD97 pKa = 3.35 SHH99 pKa = 8.18 SFALQGTAAAYY110 pKa = 7.44 GTASVDD116 pKa = 4.64 ADD118 pKa = 4.13 GTWHH122 pKa = 5.39 YY123 pKa = 9.39 TVHH126 pKa = 7.81 DD127 pKa = 4.25 SGAVDD132 pKa = 3.38 ALGAGEE138 pKa = 4.16 TLGDD142 pKa = 3.86 SFTVRR147 pKa = 11.84 VSDD150 pKa = 3.44 NHH152 pKa = 6.15 GGYY155 pKa = 10.01 VDD157 pKa = 3.66 QLVSVTITGTNDD169 pKa = 2.88 AAVITGTSSASLTEE183 pKa = 4.24 SNAAQSTSGTLSISDD198 pKa = 3.55 VDD200 pKa = 4.19 SPASFVVQNNAAGSNGYY217 pKa = 10.47 GKK219 pKa = 10.55 FSIDD223 pKa = 3.99 AAGHH227 pKa = 5.67 WSYY230 pKa = 11.09 TMNSAHH236 pKa = 7.23 DD237 pKa = 3.95 EE238 pKa = 4.41 FVGGTTYY245 pKa = 10.77 TDD247 pKa = 3.89 SITVSSADD255 pKa = 3.19 GTTQLLTVSILGTNDD270 pKa = 2.97 AAVITGTSSASLTEE284 pKa = 4.35 SNVAQSTSGTLSISDD299 pKa = 3.55 VDD301 pKa = 4.22 SPASFVAQNNVAGSNGYY318 pKa = 10.36 GKK320 pKa = 10.55 FSIDD324 pKa = 4.03 SAGHH328 pKa = 5.54 WSYY331 pKa = 11.15 TMNSAHH337 pKa = 7.23 DD338 pKa = 3.95 EE339 pKa = 4.41 FVGGTTYY346 pKa = 11.19 TDD348 pKa = 3.28 TLTVSSADD356 pKa = 3.16 GTTQLLTVNILGTNDD371 pKa = 3.18 AAVITGTSSTSLTEE385 pKa = 4.2 SNVAQSTSGTLSISDD400 pKa = 3.55 VDD402 pKa = 4.19 SPASFVVQTNVDD414 pKa = 3.67 GNHH417 pKa = 6.92 GYY419 pKa = 11.38 GKK421 pKa = 10.44 FSIDD425 pKa = 3.99 AAGHH429 pKa = 5.67 WSYY432 pKa = 11.09 TMNSAHH438 pKa = 7.23 DD439 pKa = 3.95 EE440 pKa = 4.41 FVGGTTYY447 pKa = 11.19 TDD449 pKa = 3.28 TLTVSSADD457 pKa = 3.16 GTTQLLTVSILGTNDD472 pKa = 2.97 AAVITGTSSASLTEE486 pKa = 4.35 SNVAQSTSGTLSISDD501 pKa = 3.55 VDD503 pKa = 4.22 SPASFVAQSNAAGSNGYY520 pKa = 10.47 GKK522 pKa = 10.55 FSIDD526 pKa = 3.99 AAGHH530 pKa = 5.67 WSYY533 pKa = 11.09 TMNSAHH539 pKa = 7.23 DD540 pKa = 3.95 EE541 pKa = 4.41 FVGGTTYY548 pKa = 10.77 TDD550 pKa = 3.89 SITVSSADD558 pKa = 3.19 GTTQLLTVSILGTNDD573 pKa = 2.97 AAVITGTSTAALTEE587 pKa = 4.61 SNVAQSTSGTLSISDD602 pKa = 3.55 VDD604 pKa = 4.19 SPASFVVQSNVAGSNGYY621 pKa = 10.51 GKK623 pKa = 10.55 FSIDD627 pKa = 3.99 AAGHH631 pKa = 5.67 WSYY634 pKa = 11.09 TMNSAHH640 pKa = 7.23 DD641 pKa = 3.95 EE642 pKa = 4.41 FVGGTTYY649 pKa = 10.77 TDD651 pKa = 3.89 SITVSSADD659 pKa = 3.19 GTTQLLTVSILGTNDD674 pKa = 3.76 APVLDD679 pKa = 5.0 LNGASAGSDD688 pKa = 3.09 TTASYY693 pKa = 7.67 PTGVSQILIAPAATVTDD710 pKa = 3.48 IDD712 pKa = 4.13 SANLQSMTVTLTNSPDD728 pKa = 3.59 SSSGAARR735 pKa = 11.84 EE736 pKa = 3.97 ILSLNATATAAAAGLTVSGVSSSNNNVYY764 pKa = 8.86 TLTISGAASVATYY777 pKa = 10.29 QSILDD782 pKa = 3.76 GVQYY786 pKa = 11.17 TDD788 pKa = 3.56 IKK790 pKa = 9.73 TGNHH794 pKa = 3.92 NTAARR799 pKa = 11.84 TVNVVVSDD807 pKa = 4.34 GSASSVLHH815 pKa = 5.66 TVTISVAAPAGVAGTPISLAIADD838 pKa = 4.52 PSLGTHH844 pKa = 6.87 DD845 pKa = 4.51 GVSTTITGVPSDD857 pKa = 3.71 WSIDD861 pKa = 3.62 GATKK865 pKa = 10.27 NANGSWTAYY874 pKa = 9.71 ISDD877 pKa = 3.92 PGMLTVTTPSGFAGAAVLTVTQTWVNADD905 pKa = 3.22 GSEE908 pKa = 3.81 GRR910 pKa = 11.84 AIILDD915 pKa = 3.68 NVEE918 pKa = 4.59 AYY920 pKa = 10.49 APGSPIFALSSDD932 pKa = 4.12 DD933 pKa = 3.95 NLTGSSAADD942 pKa = 3.14 TFVFAQPMGNNMIRR956 pKa = 11.84 NFNPANDD963 pKa = 4.54 KK964 pKa = 10.63 IDD966 pKa = 4.81 LIGFAGVGSIANLQLANDD984 pKa = 4.0 ANGNAVISIGNGQSITLLGVDD1005 pKa = 4.19 VSLLLAANFLFNQVPVLHH1023 pKa = 7.09 NDD1025 pKa = 3.14 GVMTIADD1032 pKa = 4.12 GAILPLGGEE1041 pKa = 4.28 VDD1043 pKa = 3.54 NSGSIVLASSGGATSLEE1060 pKa = 4.1 VLVDD1064 pKa = 3.8 SVTLQGGGKK1073 pKa = 9.93 LLLSDD1078 pKa = 5.18 DD1079 pKa = 3.76 SHH1081 pKa = 8.14 NSVFGGAGSATLVNVDD1097 pKa = 3.7 NTISGAGQLGGGQMTLLNHH1116 pKa = 6.42 GSIVADD1122 pKa = 4.28 GSHH1125 pKa = 7.09 ALLLDD1130 pKa = 3.37 TGGNSIVNSGQLHH1143 pKa = 6.2 ASGAGGMVVASSLEE1157 pKa = 3.85 NSGNLWADD1165 pKa = 3.24 NSSLQLQQDD1174 pKa = 3.55 VSGQGSALISGNGSIAFMAAADD1196 pKa = 3.99 TDD1198 pKa = 3.82 VSFANGSHH1206 pKa = 6.83 GDD1208 pKa = 3.82 LLLDD1212 pKa = 4.08 HH1213 pKa = 7.48 ADD1215 pKa = 3.47 AFSGRR1220 pKa = 11.84 IIGMASGDD1228 pKa = 3.88 HH1229 pKa = 6.87 LDD1231 pKa = 4.75 LGGLAYY1237 pKa = 10.74 NDD1239 pKa = 4.24 GLRR1242 pKa = 11.84 LDD1244 pKa = 4.57 YY1245 pKa = 11.01 IGDD1248 pKa = 3.62 SSGIGGKK1255 pKa = 9.84 LLIINGSDD1263 pKa = 3.3 TTSLGLIGHH1272 pKa = 5.86 YY1273 pKa = 9.27 QASDD1277 pKa = 3.57 FQLAAAADD1285 pKa = 3.78 GHH1287 pKa = 5.97 SVVNTSQTDD1296 pKa = 3.15 SSTVIGTAGDD1306 pKa = 3.73 DD1307 pKa = 3.82 TLQGSSGNDD1316 pKa = 2.83 ILLAGAGHH1324 pKa = 6.0 NTLTGGGGSDD1334 pKa = 3.32 TYY1336 pKa = 11.81 VLLRR1340 pKa = 11.84 SDD1342 pKa = 3.92 GNAVDD1347 pKa = 4.87 TITDD1351 pKa = 4.19 FKK1353 pKa = 11.1 PGDD1356 pKa = 4.16 GGDD1359 pKa = 3.51 KK1360 pKa = 10.83 LQIGDD1365 pKa = 4.95 LLTGYY1370 pKa = 10.31 QPDD1373 pKa = 3.67 AANQFLSLRR1382 pKa = 11.84 EE1383 pKa = 4.09 EE1384 pKa = 4.3 NGNTVISVDD1393 pKa = 3.41 RR1394 pKa = 11.84 DD1395 pKa = 3.58 GAGGKK1400 pKa = 9.09 YY1401 pKa = 9.12 QMQDD1405 pKa = 2.89 MVVLQGVTGLDD1416 pKa = 3.22 LTTLLKK1422 pKa = 10.77 HH1423 pKa = 6.39 VDD1425 pKa = 3.2 TSQII1429 pKa = 3.31
Molecular weight: 143.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.666
IPC2_protein 3.694
IPC_protein 3.77
Toseland 3.528
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.592
Grimsley 3.427
Solomon 3.795
Lehninger 3.745
Nozaki 3.897
DTASelect 4.24
Thurlkill 3.592
EMBOSS 3.77
Sillero 3.91
Patrickios 1.062
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1C6M0F0|A0A1C6M0F0_9NEIS Uncharacterized protein OS=Vogesella sp. LIG4 OX=1192162 GN=PSELUDRAFT_1090 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.29 QPSNTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.05 RR14 pKa = 11.84 THH16 pKa = 5.88 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3722
0
3722
1207113
26
2768
324.3
35.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.146 ± 0.059
1.01 ± 0.015
5.274 ± 0.028
5.245 ± 0.038
3.5 ± 0.021
7.984 ± 0.039
2.404 ± 0.021
4.618 ± 0.034
3.338 ± 0.04
11.79 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.019
2.892 ± 0.027
4.878 ± 0.034
4.799 ± 0.039
6.358 ± 0.039
5.626 ± 0.037
4.627 ± 0.033
7.122 ± 0.038
1.402 ± 0.016
2.519 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here