Vogesella sp. LIG4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Chromobacteriaceae; Vogesella; unclassified Vogesella

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C6MT89|A0A1C6MT89_9NEIS Acyl-CoA reductase OS=Vogesella sp. LIG4 OX=1192162 GN=PSELUDRAFT_2512 PE=3 SV=1
SS1 pKa = 7.15ASVDD5 pKa = 3.56ADD7 pKa = 4.01GTWHH11 pKa = 5.47YY12 pKa = 9.31TVSDD16 pKa = 3.86SGTVDD21 pKa = 3.17ALAAGEE27 pKa = 4.13TLGDD31 pKa = 3.79SFTVRR36 pKa = 11.84VSDD39 pKa = 3.43NHH41 pKa = 6.89GGFVDD46 pKa = 3.6QLVSVTITGTNDD58 pKa = 2.83APLIDD63 pKa = 4.54AANTTASGGVSEE75 pKa = 5.68GDD77 pKa = 3.8DD78 pKa = 3.28NSSRR82 pKa = 11.84STSGVIAYY90 pKa = 10.36SDD92 pKa = 3.39VDD94 pKa = 3.5ASDD97 pKa = 3.35SHH99 pKa = 8.18SFALQGTAAAYY110 pKa = 7.44GTASVDD116 pKa = 4.64ADD118 pKa = 4.13GTWHH122 pKa = 5.39YY123 pKa = 9.39TVHH126 pKa = 7.81DD127 pKa = 4.25SGAVDD132 pKa = 3.38ALGAGEE138 pKa = 4.16TLGDD142 pKa = 3.86SFTVRR147 pKa = 11.84VSDD150 pKa = 3.44NHH152 pKa = 6.15GGYY155 pKa = 10.01VDD157 pKa = 3.66QLVSVTITGTNDD169 pKa = 2.88AAVITGTSSASLTEE183 pKa = 4.24SNAAQSTSGTLSISDD198 pKa = 3.55VDD200 pKa = 4.19SPASFVVQNNAAGSNGYY217 pKa = 10.47GKK219 pKa = 10.55FSIDD223 pKa = 3.99AAGHH227 pKa = 5.67WSYY230 pKa = 11.09TMNSAHH236 pKa = 7.23DD237 pKa = 3.95EE238 pKa = 4.41FVGGTTYY245 pKa = 10.77TDD247 pKa = 3.89SITVSSADD255 pKa = 3.19GTTQLLTVSILGTNDD270 pKa = 2.97AAVITGTSSASLTEE284 pKa = 4.35SNVAQSTSGTLSISDD299 pKa = 3.55VDD301 pKa = 4.22SPASFVAQNNVAGSNGYY318 pKa = 10.36GKK320 pKa = 10.55FSIDD324 pKa = 4.03SAGHH328 pKa = 5.54WSYY331 pKa = 11.15TMNSAHH337 pKa = 7.23DD338 pKa = 3.95EE339 pKa = 4.41FVGGTTYY346 pKa = 11.19TDD348 pKa = 3.28TLTVSSADD356 pKa = 3.16GTTQLLTVNILGTNDD371 pKa = 3.18AAVITGTSSTSLTEE385 pKa = 4.2SNVAQSTSGTLSISDD400 pKa = 3.55VDD402 pKa = 4.19SPASFVVQTNVDD414 pKa = 3.67GNHH417 pKa = 6.92GYY419 pKa = 11.38GKK421 pKa = 10.44FSIDD425 pKa = 3.99AAGHH429 pKa = 5.67WSYY432 pKa = 11.09TMNSAHH438 pKa = 7.23DD439 pKa = 3.95EE440 pKa = 4.41FVGGTTYY447 pKa = 11.19TDD449 pKa = 3.28TLTVSSADD457 pKa = 3.16GTTQLLTVSILGTNDD472 pKa = 2.97AAVITGTSSASLTEE486 pKa = 4.35SNVAQSTSGTLSISDD501 pKa = 3.55VDD503 pKa = 4.22SPASFVAQSNAAGSNGYY520 pKa = 10.47GKK522 pKa = 10.55FSIDD526 pKa = 3.99AAGHH530 pKa = 5.67WSYY533 pKa = 11.09TMNSAHH539 pKa = 7.23DD540 pKa = 3.95EE541 pKa = 4.41FVGGTTYY548 pKa = 10.77TDD550 pKa = 3.89SITVSSADD558 pKa = 3.19GTTQLLTVSILGTNDD573 pKa = 2.97AAVITGTSTAALTEE587 pKa = 4.61SNVAQSTSGTLSISDD602 pKa = 3.55VDD604 pKa = 4.19SPASFVVQSNVAGSNGYY621 pKa = 10.51GKK623 pKa = 10.55FSIDD627 pKa = 3.99AAGHH631 pKa = 5.67WSYY634 pKa = 11.09TMNSAHH640 pKa = 7.23DD641 pKa = 3.95EE642 pKa = 4.41FVGGTTYY649 pKa = 10.77TDD651 pKa = 3.89SITVSSADD659 pKa = 3.19GTTQLLTVSILGTNDD674 pKa = 3.76APVLDD679 pKa = 5.0LNGASAGSDD688 pKa = 3.09TTASYY693 pKa = 7.67PTGVSQILIAPAATVTDD710 pKa = 3.48IDD712 pKa = 4.13SANLQSMTVTLTNSPDD728 pKa = 3.59SSSGAARR735 pKa = 11.84EE736 pKa = 3.97ILSLNATATAAAAGLTVSGVSSSNNNVYY764 pKa = 8.86TLTISGAASVATYY777 pKa = 10.29QSILDD782 pKa = 3.76GVQYY786 pKa = 11.17TDD788 pKa = 3.56IKK790 pKa = 9.73TGNHH794 pKa = 3.92NTAARR799 pKa = 11.84TVNVVVSDD807 pKa = 4.34GSASSVLHH815 pKa = 5.66TVTISVAAPAGVAGTPISLAIADD838 pKa = 4.52PSLGTHH844 pKa = 6.87DD845 pKa = 4.51GVSTTITGVPSDD857 pKa = 3.71WSIDD861 pKa = 3.62GATKK865 pKa = 10.27NANGSWTAYY874 pKa = 9.71ISDD877 pKa = 3.92PGMLTVTTPSGFAGAAVLTVTQTWVNADD905 pKa = 3.22GSEE908 pKa = 3.81GRR910 pKa = 11.84AIILDD915 pKa = 3.68NVEE918 pKa = 4.59AYY920 pKa = 10.49APGSPIFALSSDD932 pKa = 4.12DD933 pKa = 3.95NLTGSSAADD942 pKa = 3.14TFVFAQPMGNNMIRR956 pKa = 11.84NFNPANDD963 pKa = 4.54KK964 pKa = 10.63IDD966 pKa = 4.81LIGFAGVGSIANLQLANDD984 pKa = 4.0ANGNAVISIGNGQSITLLGVDD1005 pKa = 4.19VSLLLAANFLFNQVPVLHH1023 pKa = 7.09NDD1025 pKa = 3.14GVMTIADD1032 pKa = 4.12GAILPLGGEE1041 pKa = 4.28VDD1043 pKa = 3.54NSGSIVLASSGGATSLEE1060 pKa = 4.1VLVDD1064 pKa = 3.8SVTLQGGGKK1073 pKa = 9.93LLLSDD1078 pKa = 5.18DD1079 pKa = 3.76SHH1081 pKa = 8.14NSVFGGAGSATLVNVDD1097 pKa = 3.7NTISGAGQLGGGQMTLLNHH1116 pKa = 6.42GSIVADD1122 pKa = 4.28GSHH1125 pKa = 7.09ALLLDD1130 pKa = 3.37TGGNSIVNSGQLHH1143 pKa = 6.2ASGAGGMVVASSLEE1157 pKa = 3.85NSGNLWADD1165 pKa = 3.24NSSLQLQQDD1174 pKa = 3.55VSGQGSALISGNGSIAFMAAADD1196 pKa = 3.99TDD1198 pKa = 3.82VSFANGSHH1206 pKa = 6.83GDD1208 pKa = 3.82LLLDD1212 pKa = 4.08HH1213 pKa = 7.48ADD1215 pKa = 3.47AFSGRR1220 pKa = 11.84IIGMASGDD1228 pKa = 3.88HH1229 pKa = 6.87LDD1231 pKa = 4.75LGGLAYY1237 pKa = 10.74NDD1239 pKa = 4.24GLRR1242 pKa = 11.84LDD1244 pKa = 4.57YY1245 pKa = 11.01IGDD1248 pKa = 3.62SSGIGGKK1255 pKa = 9.84LLIINGSDD1263 pKa = 3.3TTSLGLIGHH1272 pKa = 5.86YY1273 pKa = 9.27QASDD1277 pKa = 3.57FQLAAAADD1285 pKa = 3.78GHH1287 pKa = 5.97SVVNTSQTDD1296 pKa = 3.15SSTVIGTAGDD1306 pKa = 3.73DD1307 pKa = 3.82TLQGSSGNDD1316 pKa = 2.83ILLAGAGHH1324 pKa = 6.0NTLTGGGGSDD1334 pKa = 3.32TYY1336 pKa = 11.81VLLRR1340 pKa = 11.84SDD1342 pKa = 3.92GNAVDD1347 pKa = 4.87TITDD1351 pKa = 4.19FKK1353 pKa = 11.1PGDD1356 pKa = 4.16GGDD1359 pKa = 3.51KK1360 pKa = 10.83LQIGDD1365 pKa = 4.95LLTGYY1370 pKa = 10.31QPDD1373 pKa = 3.67AANQFLSLRR1382 pKa = 11.84EE1383 pKa = 4.09EE1384 pKa = 4.3NGNTVISVDD1393 pKa = 3.41RR1394 pKa = 11.84DD1395 pKa = 3.58GAGGKK1400 pKa = 9.09YY1401 pKa = 9.12QMQDD1405 pKa = 2.89MVVLQGVTGLDD1416 pKa = 3.22LTTLLKK1422 pKa = 10.77HH1423 pKa = 6.39VDD1425 pKa = 3.2TSQII1429 pKa = 3.31

Molecular weight: 143.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C6M0F0|A0A1C6M0F0_9NEIS Uncharacterized protein OS=Vogesella sp. LIG4 OX=1192162 GN=PSELUDRAFT_1090 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.29QPSNTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.05RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3722

0

3722

1207113

26

2768

324.3

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.146 ± 0.059

1.01 ± 0.015

5.274 ± 0.028

5.245 ± 0.038

3.5 ± 0.021

7.984 ± 0.039

2.404 ± 0.021

4.618 ± 0.034

3.338 ± 0.04

11.79 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.019

2.892 ± 0.027

4.878 ± 0.034

4.799 ± 0.039

6.358 ± 0.039

5.626 ± 0.037

4.627 ± 0.033

7.122 ± 0.038

1.402 ± 0.016

2.519 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski