Amino acid dipepetide frequency for Vogesella sp. LIG4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.795AlaAla: 17.795 ± 0.175
1.296AlaCys: 1.296 ± 0.036
6.512AlaAsp: 6.512 ± 0.083
6.939AlaGlu: 6.939 ± 0.082
3.695AlaPhe: 3.695 ± 0.067
10.611AlaGly: 10.611 ± 0.101
2.428AlaHis: 2.428 ± 0.051
5.53AlaIle: 5.53 ± 0.076
3.464AlaLys: 3.464 ± 0.07
14.247AlaLeu: 14.247 ± 0.15
3.489AlaMet: 3.489 ± 0.055
3.415AlaAsn: 3.415 ± 0.058
4.943AlaPro: 4.943 ± 0.075
5.074AlaGln: 5.074 ± 0.075
7.921AlaArg: 7.921 ± 0.095
6.73AlaSer: 6.73 ± 0.097
5.198AlaThr: 5.198 ± 0.073
8.003AlaVal: 8.003 ± 0.092
1.663AlaTrp: 1.663 ± 0.041
2.507AlaTyr: 2.507 ± 0.04
0.001AlaXaa: 0.001 ± 0.001
Cys
1.042CysAla: 1.042 ± 0.033
0.149CysCys: 0.149 ± 0.013
0.558CysAsp: 0.558 ± 0.022
0.532CysGlu: 0.532 ± 0.023
0.322CysPhe: 0.322 ± 0.017
1.066CysGly: 1.066 ± 0.035
0.334CysHis: 0.334 ± 0.016
0.397CysIle: 0.397 ± 0.021
0.268CysLys: 0.268 ± 0.015
1.084CysLeu: 1.084 ± 0.032
0.204CysMet: 0.204 ± 0.012
0.297CysAsn: 0.297 ± 0.015
0.502CysPro: 0.502 ± 0.02
0.453CysGln: 0.453 ± 0.019
0.729CysArg: 0.729 ± 0.027
0.576CysSer: 0.576 ± 0.021
0.463CysThr: 0.463 ± 0.021
0.663CysVal: 0.663 ± 0.027
0.163CysTrp: 0.163 ± 0.01
0.297CysTyr: 0.297 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.56AspAla: 6.56 ± 0.083
0.571AspCys: 0.571 ± 0.02
2.887AspAsp: 2.887 ± 0.051
3.2AspGlu: 3.2 ± 0.059
2.061AspPhe: 2.061 ± 0.046
4.785AspGly: 4.785 ± 0.075
1.053AspHis: 1.053 ± 0.034
3.131AspIle: 3.131 ± 0.048
2.134AspLys: 2.134 ± 0.049
4.647AspLeu: 4.647 ± 0.064
1.418AspMet: 1.418 ± 0.032
1.654AspAsn: 1.654 ± 0.039
2.373AspPro: 2.373 ± 0.053
1.556AspGln: 1.556 ± 0.041
2.604AspArg: 2.604 ± 0.049
3.097AspSer: 3.097 ± 0.066
2.593AspThr: 2.593 ± 0.043
3.686AspVal: 3.686 ± 0.057
0.971AspTrp: 0.971 ± 0.032
1.76AspTyr: 1.76 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
6.437GluAla: 6.437 ± 0.09
0.429GluCys: 0.429 ± 0.019
2.212GluAsp: 2.212 ± 0.042
2.754GluGlu: 2.754 ± 0.056
1.741GluPhe: 1.741 ± 0.044
3.24GluGly: 3.24 ± 0.056
1.498GluHis: 1.498 ± 0.039
2.7GluIle: 2.7 ± 0.052
1.893GluLys: 1.893 ± 0.047
6.779GluLeu: 6.779 ± 0.092
1.331GluMet: 1.331 ± 0.038
1.359GluAsn: 1.359 ± 0.033
2.087GluPro: 2.087 ± 0.046
3.434GluGln: 3.434 ± 0.063
3.992GluArg: 3.992 ± 0.063
2.304GluSer: 2.304 ± 0.051
2.329GluThr: 2.329 ± 0.041
4.138GluVal: 4.138 ± 0.059
0.669GluTrp: 0.669 ± 0.024
1.127GluTyr: 1.127 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.329PheAla: 4.329 ± 0.063
0.44PheCys: 0.44 ± 0.02
2.494PheAsp: 2.494 ± 0.05
1.847PheGlu: 1.847 ± 0.036
1.296PhePhe: 1.296 ± 0.038
3.159PheGly: 3.159 ± 0.058
0.84PheHis: 0.84 ± 0.024
1.705PheIle: 1.705 ± 0.041
1.139PheLys: 1.139 ± 0.03
2.999PheLeu: 2.999 ± 0.054
0.852PheMet: 0.852 ± 0.029
1.233PheAsn: 1.233 ± 0.036
1.393PhePro: 1.393 ± 0.032
1.184PheGln: 1.184 ± 0.038
1.891PheArg: 1.891 ± 0.043
2.272PheSer: 2.272 ± 0.049
1.844PheThr: 1.844 ± 0.042
2.596PheVal: 2.596 ± 0.044
0.497PheTrp: 0.497 ± 0.022
0.993PheTyr: 0.993 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
7.311GlyAla: 7.311 ± 0.101
0.963GlyCys: 0.963 ± 0.032
3.984GlyAsp: 3.984 ± 0.068
4.713GlyGlu: 4.713 ± 0.074
3.257GlyPhe: 3.257 ± 0.06
6.371GlyGly: 6.371 ± 0.088
2.011GlyHis: 2.011 ± 0.044
4.256GlyIle: 4.256 ± 0.063
3.878GlyLys: 3.878 ± 0.06
8.471GlyLeu: 8.471 ± 0.097
2.536GlyMet: 2.536 ± 0.042
2.495GlyAsn: 2.495 ± 0.053
2.327GlyPro: 2.327 ± 0.048
3.498GlyGln: 3.498 ± 0.062
4.989GlyArg: 4.989 ± 0.063
5.08GlySer: 5.08 ± 0.082
3.539GlyThr: 3.539 ± 0.07
6.178GlyVal: 6.178 ± 0.076
1.436GlyTrp: 1.436 ± 0.033
2.547GlyTyr: 2.547 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.728HisAla: 2.728 ± 0.051
0.309HisCys: 0.309 ± 0.016
1.421HisAsp: 1.421 ± 0.04
1.169HisGlu: 1.169 ± 0.03
0.968HisPhe: 0.968 ± 0.029
2.384HisGly: 2.384 ± 0.046
0.727HisHis: 0.727 ± 0.028
1.127HisIle: 1.127 ± 0.034
0.672HisLys: 0.672 ± 0.02
2.334HisLeu: 2.334 ± 0.048
0.517HisMet: 0.517 ± 0.021
0.68HisAsn: 0.68 ± 0.021
1.507HisPro: 1.507 ± 0.039
0.947HisGln: 0.947 ± 0.025
1.338HisArg: 1.338 ± 0.034
1.35HisSer: 1.35 ± 0.036
1.069HisThr: 1.069 ± 0.03
1.487HisVal: 1.487 ± 0.035
0.454HisTrp: 0.454 ± 0.02
0.851HisTyr: 0.851 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.125IleAla: 6.125 ± 0.075
0.472IleCys: 0.472 ± 0.02
3.12IleAsp: 3.12 ± 0.048
2.77IleGlu: 2.77 ± 0.053
1.282IlePhe: 1.282 ± 0.038
4.233IleGly: 4.233 ± 0.066
1.013IleHis: 1.013 ± 0.032
1.982IleIle: 1.982 ± 0.043
1.721IleLys: 1.721 ± 0.043
3.846IleLeu: 3.846 ± 0.058
0.921IleMet: 0.921 ± 0.027
1.772IleAsn: 1.772 ± 0.038
2.051IlePro: 2.051 ± 0.041
1.433IleGln: 1.433 ± 0.038
2.922IleArg: 2.922 ± 0.053
2.81IleSer: 2.81 ± 0.056
2.687IleThr: 2.687 ± 0.05
3.474IleVal: 3.474 ± 0.061
0.447IleTrp: 0.447 ± 0.023
1.099IleTyr: 1.099 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.639LysAla: 3.639 ± 0.072
0.176LysCys: 0.176 ± 0.012
1.554LysAsp: 1.554 ± 0.041
1.6LysGlu: 1.6 ± 0.043
0.905LysPhe: 0.905 ± 0.026
2.316LysGly: 2.316 ± 0.061
0.765LysHis: 0.765 ± 0.025
1.553LysIle: 1.553 ± 0.041
1.474LysLys: 1.474 ± 0.041
4.328LysLeu: 4.328 ± 0.074
0.838LysMet: 0.838 ± 0.03
1.038LysAsn: 1.038 ± 0.024
2.05LysPro: 2.05 ± 0.056
1.75LysGln: 1.75 ± 0.044
2.118LysArg: 2.118 ± 0.046
1.77LysSer: 1.77 ± 0.039
1.717LysThr: 1.717 ± 0.04
2.673LysVal: 2.673 ± 0.06
0.354LysTrp: 0.354 ± 0.018
0.76LysTyr: 0.76 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
16.944LeuAla: 16.944 ± 0.148
1.174LeuCys: 1.174 ± 0.032
6.182LeuAsp: 6.182 ± 0.083
5.374LeuGlu: 5.374 ± 0.075
3.899LeuPhe: 3.899 ± 0.068
8.626LeuGly: 8.626 ± 0.098
2.587LeuHis: 2.587 ± 0.053
4.695LeuIle: 4.695 ± 0.069
3.853LeuLys: 3.853 ± 0.069
15.011LeuLeu: 15.011 ± 0.204
2.52LeuMet: 2.52 ± 0.041
3.069LeuAsn: 3.069 ± 0.046
7.228LeuPro: 7.228 ± 0.094
6.107LeuGln: 6.107 ± 0.098
7.032LeuArg: 7.032 ± 0.082
6.859LeuSer: 6.859 ± 0.089
5.545LeuThr: 5.545 ± 0.068
7.302LeuVal: 7.302 ± 0.078
1.436LeuTrp: 1.436 ± 0.046
2.455LeuTyr: 2.455 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.938MetAla: 2.938 ± 0.053
0.167MetCys: 0.167 ± 0.011
1.163MetAsp: 1.163 ± 0.033
1.051MetGlu: 1.051 ± 0.031
0.71MetPhe: 0.71 ± 0.028
1.692MetGly: 1.692 ± 0.045
0.558MetHis: 0.558 ± 0.022
1.032MetIle: 1.032 ± 0.033
1.03MetLys: 1.03 ± 0.027
3.169MetLeu: 3.169 ± 0.055
0.594MetMet: 0.594 ± 0.021
0.885MetAsn: 0.885 ± 0.03
1.378MetPro: 1.378 ± 0.036
1.306MetGln: 1.306 ± 0.033
1.504MetArg: 1.504 ± 0.039
1.787MetSer: 1.787 ± 0.042
1.364MetThr: 1.364 ± 0.032
1.7MetVal: 1.7 ± 0.04
0.21MetTrp: 0.21 ± 0.015
0.443MetTyr: 0.443 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.08AsnAla: 3.08 ± 0.056
0.296AsnCys: 0.296 ± 0.014
1.554AsnAsp: 1.554 ± 0.037
1.275AsnGlu: 1.275 ± 0.033
1.034AsnPhe: 1.034 ± 0.029
2.455AsnGly: 2.455 ± 0.057
0.66AsnHis: 0.66 ± 0.023
1.431AsnIle: 1.431 ± 0.036
0.956AsnLys: 0.956 ± 0.029
3.207AsnLeu: 3.207 ± 0.053
0.741AsnMet: 0.741 ± 0.024
0.963AsnAsn: 0.963 ± 0.037
1.946AsnPro: 1.946 ± 0.041
1.127AsnGln: 1.127 ± 0.036
1.839AsnArg: 1.839 ± 0.041
1.544AsnSer: 1.544 ± 0.042
1.435AsnThr: 1.435 ± 0.042
2.092AsnVal: 2.092 ± 0.041
0.453AsnTrp: 0.453 ± 0.02
0.833AsnTyr: 0.833 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
6.833ProAla: 6.833 ± 0.094
0.409ProCys: 0.409 ± 0.019
3.022ProAsp: 3.022 ± 0.055
2.903ProGlu: 2.903 ± 0.052
1.771ProPhe: 1.771 ± 0.036
3.913ProGly: 3.913 ± 0.059
1.135ProHis: 1.135 ± 0.032
1.689ProIle: 1.689 ± 0.039
1.328ProLys: 1.328 ± 0.037
5.957ProLeu: 5.957 ± 0.08
1.117ProMet: 1.117 ± 0.031
1.166ProAsn: 1.166 ± 0.032
2.363ProPro: 2.363 ± 0.06
2.652ProGln: 2.652 ± 0.057
2.613ProArg: 2.613 ± 0.063
2.239ProSer: 2.239 ± 0.05
1.973ProThr: 1.973 ± 0.045
3.729ProVal: 3.729 ± 0.056
0.716ProTrp: 0.716 ± 0.026
1.25ProTyr: 1.25 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
5.948GlnAla: 5.948 ± 0.089
0.343GlnCys: 0.343 ± 0.017
1.957GlnAsp: 1.957 ± 0.036
1.947GlnGlu: 1.947 ± 0.038
1.448GlnPhe: 1.448 ± 0.035
3.437GlnGly: 3.437 ± 0.053
1.455GlnHis: 1.455 ± 0.036
1.857GlnIle: 1.857 ± 0.039
1.25GlnLys: 1.25 ± 0.036
6.629GlnLeu: 6.629 ± 0.117
1.022GlnMet: 1.022 ± 0.028
1.02GlnAsn: 1.02 ± 0.03
2.843GlnPro: 2.843 ± 0.061
4.028GlnGln: 4.028 ± 0.099
3.721GlnArg: 3.721 ± 0.066
2.186GlnSer: 2.186 ± 0.046
1.828GlnThr: 1.828 ± 0.042
3.261GlnVal: 3.261 ± 0.053
0.728GlnTrp: 0.728 ± 0.029
1.083GlnTyr: 1.083 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
6.105ArgAla: 6.105 ± 0.079
0.601ArgCys: 0.601 ± 0.024
3.472ArgAsp: 3.472 ± 0.055
4.114ArgGlu: 4.114 ± 0.077
2.741ArgPhe: 2.741 ± 0.044
3.936ArgGly: 3.936 ± 0.059
1.937ArgHis: 1.937 ± 0.042
3.339ArgIle: 3.339 ± 0.056
2.021ArgLys: 2.021 ± 0.044
8.143ArgLeu: 8.143 ± 0.118
1.648ArgMet: 1.648 ± 0.041
1.802ArgAsn: 1.802 ± 0.041
2.652ArgPro: 2.652 ± 0.052
3.763ArgGln: 3.763 ± 0.072
4.333ArgArg: 4.333 ± 0.071
3.174ArgSer: 3.174 ± 0.059
2.44ArgThr: 2.44 ± 0.046
4.354ArgVal: 4.354 ± 0.064
0.997ArgTrp: 0.997 ± 0.027
2.01ArgTyr: 2.01 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
5.809SerAla: 5.809 ± 0.074
0.625SerCys: 0.625 ± 0.023
2.648SerAsp: 2.648 ± 0.052
2.762SerGlu: 2.762 ± 0.05
2.198SerPhe: 2.198 ± 0.051
5.548SerGly: 5.548 ± 0.09
1.43SerHis: 1.43 ± 0.035
2.701SerIle: 2.701 ± 0.054
1.778SerLys: 1.778 ± 0.04
6.578SerLeu: 6.578 ± 0.099
1.348SerMet: 1.348 ± 0.034
1.718SerAsn: 1.718 ± 0.042
2.62SerPro: 2.62 ± 0.056
2.422SerGln: 2.422 ± 0.05
3.638SerArg: 3.638 ± 0.06
3.537SerSer: 3.537 ± 0.079
2.64SerThr: 2.64 ± 0.056
3.883SerVal: 3.883 ± 0.058
0.811SerTrp: 0.811 ± 0.027
1.565SerTyr: 1.565 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.337ThrAla: 5.337 ± 0.074
0.391ThrCys: 0.391 ± 0.02
2.172ThrAsp: 2.172 ± 0.044
2.025ThrGlu: 2.025 ± 0.041
1.582ThrPhe: 1.582 ± 0.039
3.924ThrGly: 3.924 ± 0.064
1.044ThrHis: 1.044 ± 0.025
2.082ThrIle: 2.082 ± 0.049
0.983ThrLys: 0.983 ± 0.034
6.576ThrLeu: 6.576 ± 0.083
0.966ThrMet: 0.966 ± 0.028
1.08ThrAsn: 1.08 ± 0.038
3.134ThrPro: 3.134 ± 0.055
1.789ThrGln: 1.789 ± 0.043
3.035ThrArg: 3.035 ± 0.055
2.436ThrSer: 2.436 ± 0.056
2.372ThrThr: 2.372 ± 0.052
3.938ThrVal: 3.938 ± 0.065
0.496ThrTrp: 0.496 ± 0.024
0.906ThrTyr: 0.906 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
8.88ValAla: 8.88 ± 0.096
0.809ValCys: 0.809 ± 0.029
3.939ValAsp: 3.939 ± 0.054
3.923ValGlu: 3.923 ± 0.062
2.511ValPhe: 2.511 ± 0.048
5.14ValGly: 5.14 ± 0.076
1.436ValHis: 1.436 ± 0.035
3.48ValIle: 3.48 ± 0.064
2.446ValLys: 2.446 ± 0.054
8.293ValLeu: 8.293 ± 0.09
1.82ValMet: 1.82 ± 0.04
2.17ValAsn: 2.17 ± 0.046
3.289ValPro: 3.289 ± 0.057
2.769ValGln: 2.769 ± 0.047
4.259ValArg: 4.259 ± 0.061
4.424ValSer: 4.424 ± 0.071
3.532ValThr: 3.532 ± 0.059
5.594ValVal: 5.594 ± 0.082
0.848ValTrp: 0.848 ± 0.031
1.659ValTyr: 1.659 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.055TrpAla: 1.055 ± 0.032
0.175TrpCys: 0.175 ± 0.012
0.536TrpAsp: 0.536 ± 0.022
0.476TrpGlu: 0.476 ± 0.021
0.537TrpPhe: 0.537 ± 0.02
0.885TrpGly: 0.885 ± 0.026
0.444TrpHis: 0.444 ± 0.021
0.484TrpIle: 0.484 ± 0.022
0.374TrpLys: 0.374 ± 0.02
2.564TrpLeu: 2.564 ± 0.057
0.34TrpMet: 0.34 ± 0.016
0.366TrpAsn: 0.366 ± 0.017
0.713TrpPro: 0.713 ± 0.027
1.338TrpGln: 1.338 ± 0.038
1.136TrpArg: 1.136 ± 0.035
0.683TrpSer: 0.683 ± 0.026
0.448TrpThr: 0.448 ± 0.02
0.89TrpVal: 0.89 ± 0.026
0.269TrpTrp: 0.269 ± 0.02
0.311TrpTyr: 0.311 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.565TyrAla: 2.565 ± 0.052
0.302TyrCys: 0.302 ± 0.016
1.357TyrAsp: 1.357 ± 0.035
1.078TyrGlu: 1.078 ± 0.03
1.045TyrPhe: 1.045 ± 0.032
2.117TyrGly: 2.117 ± 0.039
0.683TyrHis: 0.683 ± 0.029
1.01TyrIle: 1.01 ± 0.034
0.717TyrLys: 0.717 ± 0.028
3.038TyrLeu: 3.038 ± 0.055
0.455TyrMet: 0.455 ± 0.022
0.738TyrAsn: 0.738 ± 0.029
1.378TyrPro: 1.378 ± 0.033
1.362TyrGln: 1.362 ± 0.034
1.969TyrArg: 1.969 ± 0.042
1.401TyrSer: 1.401 ± 0.034
1.254TyrThr: 1.254 ± 0.037
1.581TyrVal: 1.581 ± 0.038
0.404TyrTrp: 0.404 ± 0.019
0.736TyrTyr: 0.736 ± 0.025
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.002XaaAla: 0.002 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3722 proteins (1207113 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski