Amino acid dipepetide frequency for Actinopolyspora mzabensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.243AlaAla: 16.243 ± 0.158	0.977AlaCys: 0.977 ± 0.03	6.741AlaAsp: 6.741 ± 0.074	9.984AlaGlu: 9.984 ± 0.105	2.93AlaPhe: 2.93 ± 0.052	11.353AlaGly: 11.353 ± 0.117	2.382AlaHis: 2.382 ± 0.04	3.341AlaIle: 3.341 ± 0.05	2.108AlaLys: 2.108 ± 0.046	12.171AlaLeu: 12.171 ± 0.134	2.38AlaMet: 2.38 ± 0.047	2.302AlaAsn: 2.302 ± 0.043	5.418AlaPro: 5.418 ± 0.074	2.849AlaGln: 2.849 ± 0.045	8.995AlaArg: 8.995 ± 0.1	5.851AlaSer: 5.851 ± 0.065	6.783AlaThr: 6.783 ± 0.074	10.791AlaVal: 10.791 ± 0.106	1.417AlaTrp: 1.417 ± 0.033	1.922AlaTyr: 1.922 ± 0.034	0.001AlaXaa: 0.001 ± 0.001
Cys	0.983CysAla: 0.983 ± 0.029	0.147CysCys: 0.147 ± 0.011	0.492CysAsp: 0.492 ± 0.021	0.482CysGlu: 0.482 ± 0.02	0.244CysPhe: 0.244 ± 0.014	1.021CysGly: 1.021 ± 0.032	0.217CysHis: 0.217 ± 0.013	0.16CysIle: 0.16 ± 0.012	0.095CysLys: 0.095 ± 0.01	0.739CysLeu: 0.739 ± 0.027	0.129CysMet: 0.129 ± 0.009	0.145CysAsn: 0.145 ± 0.011	0.508CysPro: 0.508 ± 0.02	0.154CysGln: 0.154 ± 0.01	0.637CysArg: 0.637 ± 0.022	0.581CysSer: 0.581 ± 0.021	0.469CysThr: 0.469 ± 0.019	0.709CysVal: 0.709 ± 0.023	0.157CysTrp: 0.157 ± 0.012	0.201CysTyr: 0.201 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.893AspAla: 5.893 ± 0.072	0.422AspCys: 0.422 ± 0.019	3.205AspAsp: 3.205 ± 0.051	4.875AspGlu: 4.875 ± 0.059	1.599AspPhe: 1.599 ± 0.038	5.405AspGly: 5.405 ± 0.065	1.331AspHis: 1.331 ± 0.031	1.859AspIle: 1.859 ± 0.041	0.984AspLys: 0.984 ± 0.029	5.174AspLeu: 5.174 ± 0.067	0.915AspMet: 0.915 ± 0.024	1.226AspAsn: 1.226 ± 0.032	4.372AspPro: 4.372 ± 0.061	1.535AspGln: 1.535 ± 0.041	4.788AspArg: 4.788 ± 0.056	3.961AspSer: 3.961 ± 0.053	3.374AspThr: 3.374 ± 0.055	4.66AspVal: 4.66 ± 0.067	0.923AspTrp: 0.923 ± 0.031	1.248AspTyr: 1.248 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.756GluAla: 6.756 ± 0.083	0.464GluCys: 0.464 ± 0.019	2.904GluAsp: 2.904 ± 0.053	4.019GluGlu: 4.019 ± 0.067	2.001GluPhe: 2.001 ± 0.039	4.301GluGly: 4.301 ± 0.057	2.35GluHis: 2.35 ± 0.039	2.701GluIle: 2.701 ± 0.046	1.267GluLys: 1.267 ± 0.033	9.127GluLeu: 9.127 ± 0.106	1.265GluMet: 1.265 ± 0.031	1.522GluAsn: 1.522 ± 0.034	4.046GluPro: 4.046 ± 0.062	3.517GluGln: 3.517 ± 0.06	6.857GluArg: 6.857 ± 0.082	4.105GluSer: 4.105 ± 0.058	3.891GluThr: 3.891 ± 0.058	5.312GluVal: 5.312 ± 0.073	0.995GluTrp: 0.995 ± 0.027	1.308GluTyr: 1.308 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.448PheAla: 3.448 ± 0.055	0.271PheCys: 0.271 ± 0.014	2.193PheAsp: 2.193 ± 0.034	1.732PheGlu: 1.732 ± 0.037	0.833PhePhe: 0.833 ± 0.026	3.326PheGly: 3.326 ± 0.058	0.656PheHis: 0.656 ± 0.022	0.662PheIle: 0.662 ± 0.024	0.358PheLys: 0.358 ± 0.019	2.519PheLeu: 2.519 ± 0.046	0.366PheMet: 0.366 ± 0.017	0.549PheAsn: 0.549 ± 0.023	1.391PhePro: 1.391 ± 0.033	0.631PheGln: 0.631 ± 0.02	1.999PheArg: 1.999 ± 0.037	1.606PheSer: 1.606 ± 0.031	1.858PheThr: 1.858 ± 0.039	2.435PheVal: 2.435 ± 0.038	0.395PheTrp: 0.395 ± 0.018	0.596PheTyr: 0.596 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	9.519GlyAla: 9.519 ± 0.099	0.914GlyCys: 0.914 ± 0.026	4.706GlyAsp: 4.706 ± 0.057	6.199GlyGlu: 6.199 ± 0.068	3.01GlyPhe: 3.01 ± 0.048	8.457GlyGly: 8.457 ± 0.102	2.12GlyHis: 2.12 ± 0.043	3.722GlyIle: 3.722 ± 0.054	2.235GlyLys: 2.235 ± 0.049	8.762GlyLeu: 8.762 ± 0.098	2.439GlyMet: 2.439 ± 0.046	2.328GlyAsn: 2.328 ± 0.05	4.152GlyPro: 4.152 ± 0.049	2.756GlyGln: 2.756 ± 0.063	6.778GlyArg: 6.778 ± 0.075	6.61GlySer: 6.61 ± 0.088	6.024GlyThr: 6.024 ± 0.074	7.794GlyVal: 7.794 ± 0.09	1.7GlyTrp: 1.7 ± 0.038	2.239GlyTyr: 2.239 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.505HisAla: 2.505 ± 0.04	0.238HisCys: 0.238 ± 0.013	1.42HisAsp: 1.42 ± 0.033	1.672HisGlu: 1.672 ± 0.036	0.595HisPhe: 0.595 ± 0.023	2.59HisGly: 2.59 ± 0.047	0.699HisHis: 0.699 ± 0.026	0.632HisIle: 0.632 ± 0.023	0.277HisLys: 0.277 ± 0.016	2.107HisLeu: 2.107 ± 0.041	0.341HisMet: 0.341 ± 0.016	0.506HisAsn: 0.506 ± 0.019	1.787HisPro: 1.787 ± 0.034	0.663HisGln: 0.663 ± 0.022	2.233HisArg: 2.233 ± 0.048	1.344HisSer: 1.344 ± 0.033	1.379HisThr: 1.379 ± 0.032	1.777HisVal: 1.777 ± 0.035	0.373HisTrp: 0.373 ± 0.015	0.57HisTyr: 0.57 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	4.43IleAla: 4.43 ± 0.06	0.281IleCys: 0.281 ± 0.014	2.367IleAsp: 2.367 ± 0.045	2.28IleGlu: 2.28 ± 0.041	0.688IlePhe: 0.688 ± 0.023	3.647IleGly: 3.647 ± 0.053	0.633IleHis: 0.633 ± 0.021	0.855IleIle: 0.855 ± 0.03	0.604IleLys: 0.604 ± 0.022	2.319IleLeu: 2.319 ± 0.043	0.446IleMet: 0.446 ± 0.018	0.836IleAsn: 0.836 ± 0.023	1.893IlePro: 1.893 ± 0.037	0.64IleGln: 0.64 ± 0.022	2.437IleArg: 2.437 ± 0.044	2.086IleSer: 2.086 ± 0.04	2.447IleThr: 2.447 ± 0.046	2.746IleVal: 2.746 ± 0.046	0.358IleTrp: 0.358 ± 0.016	0.622IleTyr: 0.622 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	1.858LysAla: 1.858 ± 0.047	0.11LysCys: 0.11 ± 0.009	0.795LysAsp: 0.795 ± 0.027	0.874LysGlu: 0.874 ± 0.032	0.439LysPhe: 0.439 ± 0.017	1.218LysGly: 1.218 ± 0.039	0.46LysHis: 0.46 ± 0.019	0.754LysIle: 0.754 ± 0.026	0.494LysLys: 0.494 ± 0.024	2.071LysLeu: 2.071 ± 0.046	0.356LysMet: 0.356 ± 0.017	0.429LysAsn: 0.429 ± 0.017	1.2LysPro: 1.2 ± 0.031	0.763LysGln: 0.763 ± 0.026	1.539LysArg: 1.539 ± 0.036	1.204LysSer: 1.204 ± 0.038	1.033LysThr: 1.033 ± 0.033	1.472LysVal: 1.472 ± 0.038	0.249LysTrp: 0.249 ± 0.012	0.37LysTyr: 0.37 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	13.539LeuAla: 13.539 ± 0.13	0.86LeuCys: 0.86 ± 0.027	6.651LeuAsp: 6.651 ± 0.079	5.328LeuGlu: 5.328 ± 0.077	2.807LeuPhe: 2.807 ± 0.058	9.552LeuGly: 9.552 ± 0.095	2.41LeuHis: 2.41 ± 0.04	3.067LeuIle: 3.067 ± 0.057	1.27LeuLys: 1.27 ± 0.037	12.086LeuLeu: 12.086 ± 0.156	1.356LeuMet: 1.356 ± 0.035	1.85LeuAsn: 1.85 ± 0.035	6.183LeuPro: 6.183 ± 0.076	2.102LeuGln: 2.102 ± 0.039	10.094LeuArg: 10.094 ± 0.091	6.135LeuSer: 6.135 ± 0.068	5.887LeuThr: 5.887 ± 0.072	9.15LeuVal: 9.15 ± 0.114	1.275LeuTrp: 1.275 ± 0.034	1.717LeuTyr: 1.717 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.1MetAla: 2.1 ± 0.038	0.155MetCys: 0.155 ± 0.012	0.804MetAsp: 0.804 ± 0.022	0.716MetGlu: 0.716 ± 0.025	0.568MetPhe: 0.568 ± 0.019	1.299MetGly: 1.299 ± 0.033	0.372MetHis: 0.372 ± 0.017	0.758MetIle: 0.758 ± 0.025	0.295MetLys: 0.295 ± 0.013	2.108MetLeu: 2.108 ± 0.041	0.336MetMet: 0.336 ± 0.017	0.506MetAsn: 0.506 ± 0.02	1.109MetPro: 1.109 ± 0.025	0.441MetGln: 0.441 ± 0.016	1.705MetArg: 1.705 ± 0.031	1.658MetSer: 1.658 ± 0.036	1.519MetThr: 1.519 ± 0.032	1.387MetVal: 1.387 ± 0.036	0.206MetTrp: 0.206 ± 0.013	0.307MetTyr: 0.307 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.375AsnAla: 2.375 ± 0.044	0.202AsnCys: 0.202 ± 0.012	1.162AsnAsp: 1.162 ± 0.033	1.31AsnGlu: 1.31 ± 0.031	0.567AsnPhe: 0.567 ± 0.019	2.272AsnGly: 2.272 ± 0.045	0.535AsnHis: 0.535 ± 0.021	0.75AsnIle: 0.75 ± 0.025	0.418AsnLys: 0.418 ± 0.021	1.883AsnLeu: 1.883 ± 0.045	0.382AsnMet: 0.382 ± 0.017	0.547AsnAsn: 0.547 ± 0.022	1.534AsnPro: 1.534 ± 0.038	0.703AsnGln: 0.703 ± 0.025	1.862AsnArg: 1.862 ± 0.036	1.407AsnSer: 1.407 ± 0.033	1.419AsnThr: 1.419 ± 0.037	1.539AsnVal: 1.539 ± 0.039	0.333AsnTrp: 0.333 ± 0.017	0.499AsnTyr: 0.499 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	6.405ProAla: 6.405 ± 0.071	0.347ProCys: 0.347 ± 0.018	4.155ProAsp: 4.155 ± 0.064	5.485ProGlu: 5.485 ± 0.073	1.423ProPhe: 1.423 ± 0.033	5.593ProGly: 5.593 ± 0.07	1.34ProHis: 1.34 ± 0.034	1.501ProIle: 1.501 ± 0.034	1.052ProLys: 1.052 ± 0.03	4.936ProLeu: 4.936 ± 0.067	0.956ProMet: 0.956 ± 0.025	1.382ProAsn: 1.382 ± 0.032	3.255ProPro: 3.255 ± 0.067	1.46ProGln: 1.46 ± 0.038	3.935ProArg: 3.935 ± 0.071	3.294ProSer: 3.294 ± 0.053	3.201ProThr: 3.201 ± 0.053	5.005ProVal: 5.005 ± 0.061	0.858ProTrp: 0.858 ± 0.025	1.066ProTyr: 1.066 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.263GlnAla: 3.263 ± 0.062	0.179GlnCys: 0.179 ± 0.012	1.272GlnAsp: 1.272 ± 0.029	1.48GlnGlu: 1.48 ± 0.037	0.656GlnPhe: 0.656 ± 0.025	2.132GlnGly: 2.132 ± 0.046	0.938GlnHis: 0.938 ± 0.031	0.876GlnIle: 0.876 ± 0.027	0.372GlnLys: 0.372 ± 0.018	3.395GlnLeu: 3.395 ± 0.046	0.419GlnMet: 0.419 ± 0.018	0.554GlnAsn: 0.554 ± 0.021	1.878GlnPro: 1.878 ± 0.054	1.795GlnGln: 1.795 ± 0.054	3.401GlnArg: 3.401 ± 0.058	1.463GlnSer: 1.463 ± 0.035	1.119GlnThr: 1.119 ± 0.027	2.088GlnVal: 2.088 ± 0.037	0.488GlnTrp: 0.488 ± 0.018	0.606GlnTyr: 0.606 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.399ArgAla: 9.399 ± 0.102	0.686ArgCys: 0.686 ± 0.024	4.564ArgAsp: 4.564 ± 0.061	5.938ArgGlu: 5.938 ± 0.067	2.528ArgPhe: 2.528 ± 0.043	6.306ArgGly: 6.306 ± 0.07	2.065ArgHis: 2.065 ± 0.039	3.493ArgIle: 3.493 ± 0.042	1.792ArgLys: 1.792 ± 0.043	8.788ArgLeu: 8.788 ± 0.102	2.109ArgMet: 2.109 ± 0.035	1.884ArgAsn: 1.884 ± 0.037	4.154ArgPro: 4.154 ± 0.069	2.654ArgGln: 2.654 ± 0.046	7.855ArgArg: 7.855 ± 0.101	5.295ArgSer: 5.295 ± 0.069	5.126ArgThr: 5.126 ± 0.065	6.469ArgVal: 6.469 ± 0.071	1.397ArgTrp: 1.397 ± 0.033	1.865ArgTyr: 1.865 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.048SerAla: 7.048 ± 0.081	0.509SerCys: 0.509 ± 0.019	3.584SerAsp: 3.584 ± 0.05	4.55SerGlu: 4.55 ± 0.067	1.648SerPhe: 1.648 ± 0.034	7.619SerGly: 7.619 ± 0.086	1.153SerHis: 1.153 ± 0.028	1.737SerIle: 1.737 ± 0.035	1.172SerLys: 1.172 ± 0.028	5.223SerLeu: 5.223 ± 0.058	1.279SerMet: 1.279 ± 0.027	1.304SerAsn: 1.304 ± 0.033	3.375SerPro: 3.375 ± 0.05	1.462SerGln: 1.462 ± 0.032	4.656SerArg: 4.656 ± 0.068	4.073SerSer: 4.073 ± 0.061	3.903SerThr: 3.903 ± 0.068	4.94SerVal: 4.94 ± 0.055	1.067SerTrp: 1.067 ± 0.032	1.316SerTyr: 1.316 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	7.905ThrAla: 7.905 ± 0.088	0.422ThrCys: 0.422 ± 0.017	3.599ThrAsp: 3.599 ± 0.057	4.475ThrGlu: 4.475 ± 0.054	1.544ThrPhe: 1.544 ± 0.036	6.534ThrGly: 6.534 ± 0.07	1.202ThrHis: 1.202 ± 0.03	1.864ThrIle: 1.864 ± 0.04	1.075ThrLys: 1.075 ± 0.032	5.47ThrLeu: 5.47 ± 0.071	1.03ThrMet: 1.03 ± 0.027	1.202ThrAsn: 1.202 ± 0.034	3.69ThrPro: 3.69 ± 0.052	1.23ThrGln: 1.23 ± 0.033	4.027ThrArg: 4.027 ± 0.057	3.688ThrSer: 3.688 ± 0.065	4.08ThrThr: 4.08 ± 0.066	5.818ThrVal: 5.818 ± 0.065	0.766ThrTrp: 0.766 ± 0.026	1.048ThrTyr: 1.048 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	9.81ValAla: 9.81 ± 0.097	0.742ValCys: 0.742 ± 0.025	5.243ValAsp: 5.243 ± 0.069	5.354ValGlu: 5.354 ± 0.071	2.544ValPhe: 2.544 ± 0.041	6.849ValGly: 6.849 ± 0.079	1.985ValHis: 1.985 ± 0.042	2.849ValIle: 2.849 ± 0.045	1.255ValLys: 1.255 ± 0.038	10.4ValLeu: 10.4 ± 0.127	1.26ValMet: 1.26 ± 0.032	1.783ValAsn: 1.783 ± 0.034	4.753ValPro: 4.753 ± 0.056	1.761ValGln: 1.761 ± 0.037	7.236ValArg: 7.236 ± 0.087	5.046ValSer: 5.046 ± 0.062	5.24ValThr: 5.24 ± 0.068	7.979ValVal: 7.979 ± 0.099	1.026ValTrp: 1.026 ± 0.027	1.416ValTyr: 1.416 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.344TrpAla: 1.344 ± 0.034	0.154TrpCys: 0.154 ± 0.011	0.674TrpAsp: 0.674 ± 0.023	0.694TrpGlu: 0.694 ± 0.021	0.558TrpPhe: 0.558 ± 0.02	0.958TrpGly: 0.958 ± 0.027	0.394TrpHis: 0.394 ± 0.017	0.569TrpIle: 0.569 ± 0.018	0.267TrpLys: 0.267 ± 0.014	1.96TrpLeu: 1.96 ± 0.038	0.334TrpMet: 0.334 ± 0.016	0.431TrpAsn: 0.431 ± 0.018	0.777TrpPro: 0.777 ± 0.024	0.676TrpGln: 0.676 ± 0.022	1.382TrpArg: 1.382 ± 0.032	1.063TrpSer: 1.063 ± 0.026	0.814TrpThr: 0.814 ± 0.024	0.982TrpVal: 0.982 ± 0.029	0.428TrpTrp: 0.428 ± 0.017	0.3TrpTyr: 0.3 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.118TyrAla: 2.118 ± 0.041	0.19TyrCys: 0.19 ± 0.011	1.22TyrAsp: 1.22 ± 0.042	1.261TyrGlu: 1.261 ± 0.031	0.643TyrPhe: 0.643 ± 0.024	2.031TyrGly: 2.031 ± 0.042	0.463TyrHis: 0.463 ± 0.02	0.464TyrIle: 0.464 ± 0.02	0.296TyrLys: 0.296 ± 0.015	2.079TyrLeu: 2.079 ± 0.038	0.252TyrMet: 0.252 ± 0.014	0.415TyrAsn: 0.415 ± 0.02	1.172TyrPro: 1.172 ± 0.031	0.586TyrGln: 0.586 ± 0.021	1.955TyrArg: 1.955 ± 0.038	1.145TyrSer: 1.145 ± 0.029	1.101TyrThr: 1.101 ± 0.032	1.477TyrVal: 1.477 ± 0.033	0.347TyrTrp: 0.347 ± 0.016	0.487TyrTyr: 0.487 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.004

Statistics based on 4438 proteins (1404463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---