Thermoflavimicrobium sp. FBKL4.011

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Thermoflavimicrobium; unclassified Thermoflavimicrobium

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A364K2N6|A0A364K2N6_9BACL Uncharacterized protein OS=Thermoflavimicrobium sp. FBKL4.011 OX=2137476 GN=DL897_13535 PE=4 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84TWVDD6 pKa = 2.81KK7 pKa = 9.29DD8 pKa = 3.37TCIACGACGATAPDD22 pKa = 3.76VYY24 pKa = 10.95DD25 pKa = 3.8YY26 pKa = 11.69DD27 pKa = 4.14EE28 pKa = 6.24DD29 pKa = 5.94GIAYY33 pKa = 10.06VILDD37 pKa = 4.66DD38 pKa = 3.87NTGTAEE44 pKa = 4.13VPEE47 pKa = 4.84DD48 pKa = 3.34LHH50 pKa = 9.22DD51 pKa = 4.16SVRR54 pKa = 11.84DD55 pKa = 3.77AQEE58 pKa = 4.09GCPTDD63 pKa = 3.78SIKK66 pKa = 10.82VEE68 pKa = 4.13EE69 pKa = 4.22

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A364K3L5|A0A364K3L5_9BACL Chromosomal replication initiator protein DnaA OS=Thermoflavimicrobium sp. FBKL4.011 OX=2137476 GN=dnaA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.29QPSKK9 pKa = 9.77RR10 pKa = 11.84KK11 pKa = 9.37RR12 pKa = 11.84KK13 pKa = 8.63NVHH16 pKa = 4.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.07NGRR28 pKa = 11.84KK29 pKa = 8.03VLRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.6GRR39 pKa = 11.84KK40 pKa = 8.69VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3394

0

3394

1046417

10

6385

308.3

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.571 ± 0.043

0.859 ± 0.012

4.778 ± 0.033

7.096 ± 0.052

4.239 ± 0.032

6.606 ± 0.043

2.414 ± 0.022

7.932 ± 0.044

6.805 ± 0.05

10.301 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.021

3.752 ± 0.03

4.054 ± 0.031

4.701 ± 0.039

4.736 ± 0.038

5.915 ± 0.034

5.083 ± 0.027

6.719 ± 0.039

1.295 ± 0.019

3.497 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski