Amino acid dipepetide frequency for Thermoflavimicrobium sp. FBKL4.011

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.808AlaAla: 4.808 ± 0.106	0.681AlaCys: 0.681 ± 0.03	2.994AlaAsp: 2.994 ± 0.054	4.247AlaGlu: 4.247 ± 0.084	2.808AlaPhe: 2.808 ± 0.061	4.693AlaGly: 4.693 ± 0.091	1.488AlaHis: 1.488 ± 0.039	5.697AlaIle: 5.697 ± 0.091	4.862AlaLys: 4.862 ± 0.088	6.767AlaLeu: 6.767 ± 0.098	1.878AlaMet: 1.878 ± 0.056	2.504AlaAsn: 2.504 ± 0.054	2.055AlaPro: 2.055 ± 0.045	2.585AlaGln: 2.585 ± 0.057	3.029AlaArg: 3.029 ± 0.058	3.591AlaSer: 3.591 ± 0.063	3.324AlaThr: 3.324 ± 0.058	4.557AlaVal: 4.557 ± 0.084	0.792AlaTrp: 0.792 ± 0.025	2.353AlaTyr: 2.353 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.457CysAla: 0.457 ± 0.02	0.116CysCys: 0.116 ± 0.012	0.425CysAsp: 0.425 ± 0.021	0.496CysGlu: 0.496 ± 0.022	0.415CysPhe: 0.415 ± 0.022	0.725CysGly: 0.725 ± 0.029	0.254CysHis: 0.254 ± 0.015	0.651CysIle: 0.651 ± 0.027	0.455CysLys: 0.455 ± 0.023	0.905CysLeu: 0.905 ± 0.03	0.226CysMet: 0.226 ± 0.016	0.302CysAsn: 0.302 ± 0.018	0.442CysPro: 0.442 ± 0.019	0.381CysGln: 0.381 ± 0.021	0.432CysArg: 0.432 ± 0.019	0.55CysSer: 0.55 ± 0.021	0.469CysThr: 0.469 ± 0.023	0.457CysVal: 0.457 ± 0.022	0.118CysTrp: 0.118 ± 0.011	0.312CysTyr: 0.312 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.663AspAla: 2.663 ± 0.058	0.407AspCys: 0.407 ± 0.021	2.058AspAsp: 2.058 ± 0.067	3.785AspGlu: 3.785 ± 0.068	2.122AspPhe: 2.122 ± 0.051	3.074AspGly: 3.074 ± 0.059	1.475AspHis: 1.475 ± 0.034	3.694AspIle: 3.694 ± 0.064	2.607AspLys: 2.607 ± 0.067	5.417AspLeu: 5.417 ± 0.085	1.163AspMet: 1.163 ± 0.039	1.264AspAsn: 1.264 ± 0.04	2.443AspPro: 2.443 ± 0.053	2.986AspGln: 2.986 ± 0.063	2.519AspArg: 2.519 ± 0.057	2.316AspSer: 2.316 ± 0.057	2.029AspThr: 2.029 ± 0.047	3.127AspVal: 3.127 ± 0.067	0.834AspTrp: 0.834 ± 0.027	1.796AspTyr: 1.796 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	4.917GluAla: 4.917 ± 0.078	0.458GluCys: 0.458 ± 0.019	3.1GluAsp: 3.1 ± 0.062	6.426GluGlu: 6.426 ± 0.099	2.208GluPhe: 2.208 ± 0.049	3.819GluGly: 3.819 ± 0.07	1.386GluHis: 1.386 ± 0.038	5.541GluIle: 5.541 ± 0.088	6.373GluLys: 6.373 ± 0.083	7.16GluLeu: 7.16 ± 0.106	2.163GluMet: 2.163 ± 0.046	2.822GluAsn: 2.822 ± 0.053	2.019GluPro: 2.019 ± 0.045	3.922GluGln: 3.922 ± 0.074	3.807GluArg: 3.807 ± 0.077	3.395GluSer: 3.395 ± 0.058	3.171GluThr: 3.171 ± 0.053	5.151GluVal: 5.151 ± 0.071	1.025GluTrp: 1.025 ± 0.032	2.096GluTyr: 2.096 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	2.911PheAla: 2.911 ± 0.059	0.422PheCys: 0.422 ± 0.021	2.23PheAsp: 2.23 ± 0.056	2.403PheGlu: 2.403 ± 0.055	2.174PhePhe: 2.174 ± 0.065	2.942PheGly: 2.942 ± 0.066	1.178PheHis: 1.178 ± 0.038	3.588PheIle: 3.588 ± 0.071	1.978PheLys: 1.978 ± 0.047	4.489PheLeu: 4.489 ± 0.097	1.032PheMet: 1.032 ± 0.029	1.38PheAsn: 1.38 ± 0.04	1.802PhePro: 1.802 ± 0.044	1.795PheGln: 1.795 ± 0.046	1.862PheArg: 1.862 ± 0.045	2.895PheSer: 2.895 ± 0.061	2.401PheThr: 2.401 ± 0.045	2.769PheVal: 2.769 ± 0.054	0.529PheTrp: 0.529 ± 0.026	1.607PheTyr: 1.607 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.36GlyAla: 4.36 ± 0.081	0.722GlyCys: 0.722 ± 0.028	2.885GlyAsp: 2.885 ± 0.071	4.231GlyGlu: 4.231 ± 0.08	3.036GlyPhe: 3.036 ± 0.066	4.524GlyGly: 4.524 ± 0.082	1.417GlyHis: 1.417 ± 0.043	5.905GlyIle: 5.905 ± 0.08	5.24GlyLys: 5.24 ± 0.08	6.572GlyLeu: 6.572 ± 0.1	2.036GlyMet: 2.036 ± 0.052	2.39GlyAsn: 2.39 ± 0.056	1.748GlyPro: 1.748 ± 0.044	2.342GlyGln: 2.342 ± 0.047	2.947GlyArg: 2.947 ± 0.06	3.705GlySer: 3.705 ± 0.056	3.618GlyThr: 3.618 ± 0.067	4.866GlyVal: 4.866 ± 0.073	0.96GlyTrp: 0.96 ± 0.034	2.557GlyTyr: 2.557 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.425HisAla: 1.425 ± 0.04	0.229HisCys: 0.229 ± 0.015	1.068HisAsp: 1.068 ± 0.036	1.443HisGlu: 1.443 ± 0.033	1.227HisPhe: 1.227 ± 0.037	1.535HisGly: 1.535 ± 0.037	0.824HisHis: 0.824 ± 0.04	1.86HisIle: 1.86 ± 0.056	1.045HisLys: 1.045 ± 0.033	2.836HisLeu: 2.836 ± 0.067	0.564HisMet: 0.564 ± 0.022	0.655HisAsn: 0.655 ± 0.025	1.528HisPro: 1.528 ± 0.038	1.349HisGln: 1.349 ± 0.038	1.238HisArg: 1.238 ± 0.033	1.375HisSer: 1.375 ± 0.042	1.127HisThr: 1.127 ± 0.034	1.553HisVal: 1.553 ± 0.039	0.334HisTrp: 0.334 ± 0.018	0.93HisTyr: 0.93 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.925IleAla: 5.925 ± 0.094	0.82IleCys: 0.82 ± 0.029	4.133IleAsp: 4.133 ± 0.072	5.542IleGlu: 5.542 ± 0.083	3.226IlePhe: 3.226 ± 0.068	5.789IleGly: 5.789 ± 0.097	2.227IleHis: 2.227 ± 0.05	5.368IleIle: 5.368 ± 0.092	4.281IleLys: 4.281 ± 0.064	7.754IleLeu: 7.754 ± 0.119	1.631IleMet: 1.631 ± 0.041	2.755IleAsn: 2.755 ± 0.058	3.881IlePro: 3.881 ± 0.066	4.106IleGln: 4.106 ± 0.074	4.123IleArg: 4.123 ± 0.066	5.186IleSer: 5.186 ± 0.073	4.113IleThr: 4.113 ± 0.071	4.769IleVal: 4.769 ± 0.077	0.951IleTrp: 0.951 ± 0.03	2.74IleTyr: 2.74 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	4.197LysAla: 4.197 ± 0.065	0.422LysCys: 0.422 ± 0.023	3.369LysAsp: 3.369 ± 0.071	6.564LysGlu: 6.564 ± 0.101	1.635LysPhe: 1.635 ± 0.041	4.169LysGly: 4.169 ± 0.066	1.454LysHis: 1.454 ± 0.046	4.521LysIle: 4.521 ± 0.075	6.043LysLys: 6.043 ± 0.125	5.897LysLeu: 5.897 ± 0.085	2.054LysMet: 2.054 ± 0.046	2.929LysAsn: 2.929 ± 0.064	2.575LysPro: 2.575 ± 0.063	3.804LysGln: 3.804 ± 0.073	3.759LysArg: 3.759 ± 0.062	3.431LysSer: 3.431 ± 0.065	3.056LysThr: 3.056 ± 0.051	4.791LysVal: 4.791 ± 0.072	1.131LysTrp: 1.131 ± 0.042	2.244LysTyr: 2.244 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	7.617LeuAla: 7.617 ± 0.099	0.889LeuCys: 0.889 ± 0.036	5.153LeuAsp: 5.153 ± 0.071	6.893LeuGlu: 6.893 ± 0.086	4.864LeuPhe: 4.864 ± 0.09	6.973LeuGly: 6.973 ± 0.101	2.223LeuHis: 2.223 ± 0.049	7.871LeuIle: 7.871 ± 0.114	6.636LeuLys: 6.636 ± 0.09	10.57LeuLeu: 10.57 ± 0.134	2.322LeuMet: 2.322 ± 0.048	3.874LeuAsn: 3.874 ± 0.07	4.524LeuPro: 4.524 ± 0.075	4.429LeuGln: 4.429 ± 0.081	4.443LeuArg: 4.443 ± 0.076	7.131LeuSer: 7.131 ± 0.093	5.636LeuThr: 5.636 ± 0.084	6.577LeuVal: 6.577 ± 0.084	1.093LeuTrp: 1.093 ± 0.035	3.29LeuTyr: 3.29 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.77MetAla: 1.77 ± 0.043	0.155MetCys: 0.155 ± 0.011	1.412MetAsp: 1.412 ± 0.036	1.922MetGlu: 1.922 ± 0.045	0.884MetPhe: 0.884 ± 0.032	1.896MetGly: 1.896 ± 0.051	0.467MetHis: 0.467 ± 0.02	2.49MetIle: 2.49 ± 0.045	2.171MetLys: 2.171 ± 0.047	2.315MetLeu: 2.315 ± 0.051	0.83MetMet: 0.83 ± 0.033	1.345MetAsn: 1.345 ± 0.041	0.858MetPro: 0.858 ± 0.036	0.96MetGln: 0.96 ± 0.033	1.151MetArg: 1.151 ± 0.038	1.61MetSer: 1.61 ± 0.042	1.386MetThr: 1.386 ± 0.035	1.931MetVal: 1.931 ± 0.043	0.223MetTrp: 0.223 ± 0.014	0.682MetTyr: 0.682 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.078AsnAla: 2.078 ± 0.048	0.318AsnCys: 0.318 ± 0.019	1.71AsnAsp: 1.71 ± 0.042	2.611AsnGlu: 2.611 ± 0.054	1.253AsnPhe: 1.253 ± 0.032	2.452AsnGly: 2.452 ± 0.05	1.19AsnHis: 1.19 ± 0.036	2.675AsnIle: 2.675 ± 0.05	2.467AsnLys: 2.467 ± 0.063	3.724AsnLeu: 3.724 ± 0.061	0.988AsnMet: 0.988 ± 0.03	1.481AsnAsn: 1.481 ± 0.06	2.143AsnPro: 2.143 ± 0.054	2.517AsnGln: 2.517 ± 0.055	2.07AsnArg: 2.07 ± 0.05	1.966AsnSer: 1.966 ± 0.048	1.806AsnThr: 1.806 ± 0.044	2.089AsnVal: 2.089 ± 0.045	0.588AsnTrp: 0.588 ± 0.022	1.397AsnTyr: 1.397 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	2.293ProAla: 2.293 ± 0.053	0.257ProCys: 0.257 ± 0.019	2.26ProAsp: 2.26 ± 0.049	3.186ProGlu: 3.186 ± 0.057	2.043ProPhe: 2.043 ± 0.043	2.257ProGly: 2.257 ± 0.052	1.09ProHis: 1.09 ± 0.028	3.444ProIle: 3.444 ± 0.058	2.71ProLys: 2.71 ± 0.05	3.989ProLeu: 3.989 ± 0.078	0.961ProMet: 0.961 ± 0.032	1.805ProAsn: 1.805 ± 0.035	1.4ProPro: 1.4 ± 0.045	1.517ProGln: 1.517 ± 0.042	1.499ProArg: 1.499 ± 0.038	2.65ProSer: 2.65 ± 0.056	2.301ProThr: 2.301 ± 0.058	2.814ProVal: 2.814 ± 0.051	0.497ProTrp: 0.497 ± 0.026	1.566ProTyr: 1.566 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.6GlnAla: 3.6 ± 0.055	0.27GlnCys: 0.27 ± 0.016	1.945GlnAsp: 1.945 ± 0.054	3.579GlnGlu: 3.579 ± 0.077	1.742GlnPhe: 1.742 ± 0.045	2.762GlnGly: 2.762 ± 0.052	0.972GlnHis: 0.972 ± 0.029	3.786GlnIle: 3.786 ± 0.066	3.6GlnLys: 3.6 ± 0.064	5.126GlnLeu: 5.126 ± 0.087	1.364GlnMet: 1.364 ± 0.039	1.761GlnAsn: 1.761 ± 0.046	1.663GlnPro: 1.663 ± 0.039	2.339GlnGln: 2.339 ± 0.059	1.969GlnArg: 1.969 ± 0.038	2.615GlnSer: 2.615 ± 0.059	2.44GlnThr: 2.44 ± 0.051	3.637GlnVal: 3.637 ± 0.067	0.491GlnTrp: 0.491 ± 0.026	1.353GlnTyr: 1.353 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.712ArgAla: 2.712 ± 0.054	0.375ArgCys: 0.375 ± 0.016	2.17ArgAsp: 2.17 ± 0.045	3.426ArgGlu: 3.426 ± 0.071	2.327ArgPhe: 2.327 ± 0.049	2.674ArgGly: 2.674 ± 0.059	0.944ArgHis: 0.944 ± 0.03	3.829ArgIle: 3.829 ± 0.069	3.637ArgLys: 3.637 ± 0.066	5.247ArgLeu: 5.247 ± 0.086	1.423ArgMet: 1.423 ± 0.035	1.911ArgAsn: 1.911 ± 0.045	1.644ArgPro: 1.644 ± 0.041	2.133ArgGln: 2.133 ± 0.049	2.384ArgArg: 2.384 ± 0.056	2.709ArgSer: 2.709 ± 0.051	2.178ArgThr: 2.178 ± 0.047	3.144ArgVal: 3.144 ± 0.058	0.622ArgTrp: 0.622 ± 0.022	1.867ArgTyr: 1.867 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	3.206SerAla: 3.206 ± 0.059	0.484SerCys: 0.484 ± 0.027	2.694SerAsp: 2.694 ± 0.052	3.549SerGlu: 3.549 ± 0.07	3.136SerPhe: 3.136 ± 0.062	4.097SerGly: 4.097 ± 0.063	1.484SerHis: 1.484 ± 0.036	4.895SerIle: 4.895 ± 0.089	3.744SerLys: 3.744 ± 0.065	6.716SerLeu: 6.716 ± 0.086	1.693SerMet: 1.693 ± 0.041	2.146SerAsn: 2.146 ± 0.051	2.514SerPro: 2.514 ± 0.051	2.441SerGln: 2.441 ± 0.046	2.526SerArg: 2.526 ± 0.052	4.07SerSer: 4.07 ± 0.081	3.027SerThr: 3.027 ± 0.054	3.654SerVal: 3.654 ± 0.061	0.76SerTrp: 0.76 ± 0.029	2.317SerTyr: 2.317 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.457ThrAla: 3.457 ± 0.059	0.467ThrCys: 0.467 ± 0.022	2.358ThrAsp: 2.358 ± 0.048	3.094ThrGlu: 3.094 ± 0.063	2.228ThrPhe: 2.228 ± 0.052	3.897ThrGly: 3.897 ± 0.068	1.257ThrHis: 1.257 ± 0.031	4.122ThrIle: 4.122 ± 0.064	3.103ThrLys: 3.103 ± 0.058	5.263ThrLeu: 5.263 ± 0.069	1.195ThrMet: 1.195 ± 0.033	1.913ThrAsn: 1.913 ± 0.045	2.471ThrPro: 2.471 ± 0.053	1.91ThrGln: 1.91 ± 0.046	2.08ThrArg: 2.08 ± 0.049	3.152ThrSer: 3.152 ± 0.057	2.773ThrThr: 2.773 ± 0.057	3.561ThrVal: 3.561 ± 0.056	0.615ThrTrp: 0.615 ± 0.026	1.913ThrTyr: 1.913 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.685ValAla: 4.685 ± 0.085	0.665ValCys: 0.665 ± 0.029	3.515ValAsp: 3.515 ± 0.059	4.545ValGlu: 4.545 ± 0.072	2.74ValPhe: 2.74 ± 0.052	4.623ValGly: 4.623 ± 0.074	1.538ValHis: 1.538 ± 0.037	5.717ValIle: 5.717 ± 0.084	4.434ValLys: 4.434 ± 0.065	6.489ValLeu: 6.489 ± 0.079	1.637ValMet: 1.637 ± 0.044	2.538ValAsn: 2.538 ± 0.053	2.786ValPro: 2.786 ± 0.065	2.778ValGln: 2.778 ± 0.057	3.003ValArg: 3.003 ± 0.057	4.153ValSer: 4.153 ± 0.069	3.598ValThr: 3.598 ± 0.058	4.815ValVal: 4.815 ± 0.089	0.746ValTrp: 0.746 ± 0.027	2.185ValTyr: 2.185 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.7TrpAla: 0.7 ± 0.026	0.106TrpCys: 0.106 ± 0.01	0.583TrpAsp: 0.583 ± 0.027	0.843TrpGlu: 0.843 ± 0.033	0.584TrpPhe: 0.584 ± 0.029	0.809TrpGly: 0.809 ± 0.028	0.232TrpHis: 0.232 ± 0.016	1.271TrpIle: 1.271 ± 0.037	0.948TrpLys: 0.948 ± 0.026	1.702TrpLeu: 1.702 ± 0.052	0.439TrpMet: 0.439 ± 0.022	0.636TrpAsn: 0.636 ± 0.023	0.345TrpPro: 0.345 ± 0.02	0.534TrpGln: 0.534 ± 0.023	0.543TrpArg: 0.543 ± 0.027	0.742TrpSer: 0.742 ± 0.028	0.546TrpThr: 0.546 ± 0.024	0.824TrpVal: 0.824 ± 0.028	0.189TrpTrp: 0.189 ± 0.014	0.375TrpTyr: 0.375 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.932TyrAla: 1.932 ± 0.049	0.325TyrCys: 0.325 ± 0.018	1.718TyrAsp: 1.718 ± 0.041	2.173TyrGlu: 2.173 ± 0.049	1.735TyrPhe: 1.735 ± 0.047	2.35TyrGly: 2.35 ± 0.047	1.045TyrHis: 1.045 ± 0.034	2.397TyrIle: 2.397 ± 0.047	1.711TyrLys: 1.711 ± 0.044	4.072TyrLeu: 4.072 ± 0.072	0.858TyrMet: 0.858 ± 0.028	1.11TyrAsn: 1.11 ± 0.031	1.701TyrPro: 1.701 ± 0.04	2.183TyrGln: 2.183 ± 0.05	1.975TyrArg: 1.975 ± 0.049	1.911TyrSer: 1.911 ± 0.041	1.831TyrThr: 1.831 ± 0.043	2.103TyrVal: 2.103 ± 0.052	0.449TyrTrp: 0.449 ± 0.021	1.393TyrTyr: 1.393 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3394 proteins (1046417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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