Sphingomonas parva

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8ZKF2|A0A4Y8ZKF2_9SPHN NADPH-dependent 7-cyano-7-deazaguanine reductase QueF (Fragment) OS=Sphingomonas parva OX=2555898 GN=E2493_20045 PE=4 SV=1
MM1 pKa = 7.38GGSRR5 pKa = 11.84MSTEE9 pKa = 4.38LGFDD13 pKa = 3.34APASNLVPLEE23 pKa = 4.67EE24 pKa = 4.28IAHH27 pKa = 4.77TTFVEE32 pKa = 4.87GCGCAACQGFQDD44 pKa = 4.08AGTDD48 pKa = 3.94GVISKK53 pKa = 10.96GGFNEE58 pKa = 4.2ANPEE62 pKa = 4.04SGGIGVLLPLVTNPDD77 pKa = 3.23GSRR80 pKa = 11.84SITGEE85 pKa = 3.97RR86 pKa = 11.84NVDD89 pKa = 3.97AILIGAKK96 pKa = 9.12WGATNLTFSFPKK108 pKa = 10.42SGSNYY113 pKa = 9.86NGTGFDD119 pKa = 3.86SNGVSIYY126 pKa = 10.77HH127 pKa = 6.11MEE129 pKa = 5.19LGAQQQAAARR139 pKa = 11.84AAFAQISAATGLTFTEE155 pKa = 4.14ITEE158 pKa = 4.3TDD160 pKa = 3.41TVHH163 pKa = 6.72ANIRR167 pKa = 11.84ISQSGDD173 pKa = 2.96NDD175 pKa = 3.36VTSAYY180 pKa = 10.54GGFPSDD186 pKa = 3.34TRR188 pKa = 11.84GVAGDD193 pKa = 2.84IWFGRR198 pKa = 11.84TSQPYY203 pKa = 9.8YY204 pKa = 10.86DD205 pKa = 4.37LAYY208 pKa = 10.03KK209 pKa = 7.78GTWGYY214 pKa = 8.84ATMMHH219 pKa = 7.03EE220 pKa = 4.66IGHH223 pKa = 5.19TMGLKK228 pKa = 10.05HH229 pKa = 6.31GHH231 pKa = 5.58QDD233 pKa = 3.44YY234 pKa = 9.86TNSDD238 pKa = 3.12LSFYY242 pKa = 10.68FGTSPRR248 pKa = 11.84RR249 pKa = 11.84GTQSLTPDD257 pKa = 3.43NDD259 pKa = 3.47GQAWSLMTYY268 pKa = 9.37TPAPFTNSNFAGEE281 pKa = 4.87KK282 pKa = 9.42VNQPQTYY289 pKa = 7.5MQYY292 pKa = 11.57DD293 pKa = 4.48LAALQYY299 pKa = 10.17MYY301 pKa = 10.94GANYY305 pKa = 7.39NTNAGDD311 pKa = 3.89TVYY314 pKa = 9.39TWSEE318 pKa = 3.99TTGEE322 pKa = 4.12MFVNGVGQGAPSGNTILLTIWDD344 pKa = 4.15GGGVDD349 pKa = 4.76TLDD352 pKa = 3.56LSNYY356 pKa = 9.71ADD358 pKa = 3.77GVTVDD363 pKa = 4.27LRR365 pKa = 11.84PGQFSTFDD373 pKa = 3.24QDD375 pKa = 3.52QLANHH380 pKa = 7.39LAYY383 pKa = 10.65QNLTALAPGNVAMSLLYY400 pKa = 11.17NNDD403 pKa = 2.66GRR405 pKa = 11.84SLIEE409 pKa = 3.83NAKK412 pKa = 10.49GGIGNDD418 pKa = 3.17VFVGNVANNVLDD430 pKa = 4.2GGAGSDD436 pKa = 3.36TVIFTNTTGINVTLNDD452 pKa = 3.23TGADD456 pKa = 3.31VVVSHH461 pKa = 7.45DD462 pKa = 4.12GEE464 pKa = 4.64TDD466 pKa = 3.32TLRR469 pKa = 11.84SIEE472 pKa = 4.38NIVGTSASDD481 pKa = 3.74TITGNSQDD489 pKa = 3.14NSLYY493 pKa = 10.75GGTGGADD500 pKa = 3.45TLSGGAGNDD509 pKa = 3.12RR510 pKa = 11.84LFGGGFTTTTTYY522 pKa = 10.73SAPSQADD529 pKa = 2.99ITKK532 pKa = 10.33AQSTNNGSIATAVATAGAFDD552 pKa = 3.98VDD554 pKa = 4.26ANPNIINSTSVPHH567 pKa = 5.71ATINATAAGGAVEE580 pKa = 4.29YY581 pKa = 10.85YY582 pKa = 10.35KK583 pKa = 10.42IEE585 pKa = 4.07VTQAGAQAVFDD596 pKa = 3.85MDD598 pKa = 4.11GNGTIGDD605 pKa = 4.16NIIEE609 pKa = 4.52LVNSTGTVLASNDD622 pKa = 4.12TGPGDD627 pKa = 4.6PGTTTNDD634 pKa = 3.45DD635 pKa = 3.44AYY637 pKa = 9.98IAYY640 pKa = 8.3TFATPGTYY648 pKa = 10.06YY649 pKa = 10.1IRR651 pKa = 11.84VGRR654 pKa = 11.84WISGSSAAQPLVAGQTYY671 pKa = 9.1QLHH674 pKa = 6.7ISLQGAAAVTTTVSANNTSSAVLDD698 pKa = 4.05GGAGNDD704 pKa = 3.81LLVGTLADD712 pKa = 4.23DD713 pKa = 4.56LLNGGSDD720 pKa = 3.6NDD722 pKa = 3.89TASFVNAFSGGSTTGVTVDD741 pKa = 4.69LNVQGVAQNTVAAGSDD757 pKa = 3.45TLSGIEE763 pKa = 3.97NLVGSGLNDD772 pKa = 3.57TLTGDD777 pKa = 3.66GNDD780 pKa = 3.53NVIEE784 pKa = 4.57GGLGNDD790 pKa = 3.64VLAGGAGVDD799 pKa = 3.51TASYY803 pKa = 10.18AGAAAGVAVSLALQGSAQNTVNAGTDD829 pKa = 3.43TLSGFEE835 pKa = 4.34NLSGSAFADD844 pKa = 3.74SLTGDD849 pKa = 3.41ASANVVSGGAGDD861 pKa = 4.05DD862 pKa = 3.84TLNPGANAAGAVDD875 pKa = 4.96LLDD878 pKa = 4.47GGAGADD884 pKa = 3.43TASFAGLGAVTVALNGAGDD903 pKa = 3.74ATATIGGAAVATLRR917 pKa = 11.84SIEE920 pKa = 4.01NLLGGANADD929 pKa = 4.25TLTGDD934 pKa = 3.56AGSNVIDD941 pKa = 4.57GGLGDD946 pKa = 5.2DD947 pKa = 4.15VLEE950 pKa = 4.92GGAGVDD956 pKa = 3.83TISFASAGSAAVTVNLATTTAQATGRR982 pKa = 11.84GSKK985 pKa = 9.37TISGFEE991 pKa = 3.92NVLTGTGNDD1000 pKa = 3.43AVTGDD1005 pKa = 3.53GGDD1008 pKa = 3.15NVFFEE1013 pKa = 4.63GRR1015 pKa = 11.84GNDD1018 pKa = 3.51VYY1020 pKa = 11.43NGAGGSDD1027 pKa = 3.36TLDD1030 pKa = 3.51YY1031 pKa = 10.91SASTSTVAMNLALATAQNTGTWGGTDD1057 pKa = 3.43TISNIEE1063 pKa = 4.19NFVGAATFANTVIGSAAANRR1083 pKa = 11.84LVGGSAVDD1091 pKa = 3.44TMEE1094 pKa = 4.19GRR1096 pKa = 11.84NGADD1100 pKa = 3.23VLIGGAGNDD1109 pKa = 3.71TLLGGTTGALDD1120 pKa = 4.42DD1121 pKa = 4.69GSADD1125 pKa = 3.65VLEE1128 pKa = 5.25GGAGSDD1134 pKa = 4.07FLSGGQGNDD1143 pKa = 2.75ILRR1146 pKa = 11.84GGDD1149 pKa = 3.56GDD1151 pKa = 4.33DD1152 pKa = 3.81SLVGG1156 pKa = 3.55

Molecular weight: 115.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8ZPS1|A0A4Y8ZPS1_9SPHN UvrABC system protein B OS=Sphingomonas parva OX=2555898 GN=uvrB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.51GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 6.76TLSAA44 pKa = 4.22

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4021

0

4021

1299525

34

5419

323.2

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.873 ± 0.064

0.758 ± 0.013

5.774 ± 0.049

5.82 ± 0.039

3.478 ± 0.022

9.22 ± 0.056

1.864 ± 0.019

4.651 ± 0.027

2.449 ± 0.027

10.145 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.02

2.372 ± 0.033

5.475 ± 0.036

2.928 ± 0.024

8.162 ± 0.052

5.273 ± 0.034

5.024 ± 0.04

7.141 ± 0.034

1.431 ± 0.017

2.086 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski