Rhodopirellula pilleata

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae;

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6AVL8|A0A5C6AVL8_9BACT Uncharacterized protein OS=Rhodopirellula pilleata OX=2714738 GN=Pla100_04540 PE=4 SV=1
MM1 pKa = 7.85LITRR5 pKa = 11.84VDD7 pKa = 3.67DD8 pKa = 3.75VDD10 pKa = 4.34FCGKK14 pKa = 8.24TVLSGGFSCDD24 pKa = 3.35FAGCAAGEE32 pKa = 4.16ACVCVIISWPLWPDD46 pKa = 3.61PVQSDD51 pKa = 4.3GLPDD55 pKa = 3.53PHH57 pKa = 8.27SDD59 pKa = 4.05LDD61 pKa = 4.1RR62 pKa = 11.84NN63 pKa = 3.81

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5ZVM4|A0A5C5ZVM4_9BACT Uncharacterized protein OS=Rhodopirellula pilleata OX=2714738 GN=Pla100_52170 PE=4 SV=1
MM1 pKa = 7.25FWLIGWIVFGFVVGLIARR19 pKa = 11.84ALMPGTQPLGFFRR32 pKa = 11.84TMLLGVAGSFIGGLIGFFIVGGSLIQSSGWIGSIIGAVILLALQIRR78 pKa = 11.84RR79 pKa = 11.84GRR81 pKa = 11.84IAAA84 pKa = 3.95

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6138

0

6138

2488680

30

11740

405.5

44.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.423 ± 0.035

1.058 ± 0.018

6.558 ± 0.043

5.924 ± 0.037

3.692 ± 0.02

7.768 ± 0.061

2.18 ± 0.023

5.358 ± 0.025

3.234 ± 0.039

9.364 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.025

3.424 ± 0.043

5.207 ± 0.036

4.002 ± 0.025

6.671 ± 0.052

6.969 ± 0.035

5.987 ± 0.063

7.185 ± 0.029

1.471 ± 0.019

2.277 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski