Amino acid dipepetide frequency for Pelagibacter phage HTVC022P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.989AlaAla: 3.989 ± 0.547
0.384AlaCys: 0.384 ± 0.185
5.14AlaAsp: 5.14 ± 0.63
5.293AlaGlu: 5.293 ± 0.866
2.838AlaPhe: 2.838 ± 0.462
5.677AlaGly: 5.677 ± 1.445
0.46AlaHis: 0.46 ± 0.165
4.909AlaIle: 4.909 ± 0.711
6.367AlaLys: 6.367 ± 1.114
5.753AlaLeu: 5.753 ± 0.916
2.455AlaMet: 2.455 ± 0.461
5.677AlaAsn: 5.677 ± 1.035
2.762AlaPro: 2.762 ± 0.509
2.608AlaGln: 2.608 ± 0.467
2.992AlaArg: 2.992 ± 0.584
6.367AlaSer: 6.367 ± 0.901
6.29AlaThr: 6.29 ± 1.239
4.373AlaVal: 4.373 ± 0.751
0.767AlaTrp: 0.767 ± 0.245
2.608AlaTyr: 2.608 ± 0.417
0.0AlaXaa: 0.0 ± 0.0
Cys
0.537CysAla: 0.537 ± 0.207
0.077CysCys: 0.077 ± 0.08
0.384CysAsp: 0.384 ± 0.15
0.614CysGlu: 0.614 ± 0.219
0.307CysPhe: 0.307 ± 0.133
0.23CysGly: 0.23 ± 0.15
0.23CysHis: 0.23 ± 0.127
0.46CysIle: 0.46 ± 0.252
0.384CysLys: 0.384 ± 0.153
0.997CysLeu: 0.997 ± 0.422
0.384CysMet: 0.384 ± 0.184
0.537CysAsn: 0.537 ± 0.239
0.153CysPro: 0.153 ± 0.114
0.384CysGln: 0.384 ± 0.166
0.537CysArg: 0.537 ± 0.232
0.307CysSer: 0.307 ± 0.155
0.537CysThr: 0.537 ± 0.182
0.384CysVal: 0.384 ± 0.165
0.153CysTrp: 0.153 ± 0.113
0.307CysTyr: 0.307 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
4.833AspAla: 4.833 ± 0.654
0.46AspCys: 0.46 ± 0.194
3.605AspAsp: 3.605 ± 0.457
2.915AspGlu: 2.915 ± 0.567
2.992AspPhe: 2.992 ± 0.503
4.449AspGly: 4.449 ± 0.602
0.46AspHis: 0.46 ± 0.184
4.909AspIle: 4.909 ± 0.499
5.37AspLys: 5.37 ± 0.723
5.677AspLeu: 5.677 ± 0.581
1.764AspMet: 1.764 ± 0.475
3.145AspAsn: 3.145 ± 0.472
1.918AspPro: 1.918 ± 0.537
1.381AspGln: 1.381 ± 0.233
2.915AspArg: 2.915 ± 0.626
3.375AspSer: 3.375 ± 0.618
4.526AspThr: 4.526 ± 0.727
3.068AspVal: 3.068 ± 0.513
0.537AspTrp: 0.537 ± 0.243
2.071AspTyr: 2.071 ± 0.34
0.0AspXaa: 0.0 ± 0.0
Glu
4.679GluAla: 4.679 ± 0.636
0.614GluCys: 0.614 ± 0.292
2.838GluAsp: 2.838 ± 0.536
4.373GluGlu: 4.373 ± 0.629
2.301GluPhe: 2.301 ± 0.671
4.373GluGly: 4.373 ± 0.64
1.151GluHis: 1.151 ± 0.378
4.066GluIle: 4.066 ± 0.521
5.14GluLys: 5.14 ± 1.098
4.756GluLeu: 4.756 ± 0.703
2.301GluMet: 2.301 ± 0.449
3.836GluAsn: 3.836 ± 0.548
1.534GluPro: 1.534 ± 0.406
2.915GluGln: 2.915 ± 0.622
3.375GluArg: 3.375 ± 0.635
2.531GluSer: 2.531 ± 0.394
4.449GluThr: 4.449 ± 0.643
3.912GluVal: 3.912 ± 0.541
0.921GluTrp: 0.921 ± 0.334
2.301GluTyr: 2.301 ± 0.502
0.0GluXaa: 0.0 ± 0.0
Phe
2.455PheAla: 2.455 ± 0.412
0.537PheCys: 0.537 ± 0.247
3.068PheAsp: 3.068 ± 0.613
2.531PheGlu: 2.531 ± 0.482
1.304PhePhe: 1.304 ± 0.321
2.762PheGly: 2.762 ± 0.51
0.614PheHis: 0.614 ± 0.276
2.301PheIle: 2.301 ± 0.408
3.682PheLys: 3.682 ± 0.531
2.455PheLeu: 2.455 ± 0.499
1.304PheMet: 1.304 ± 0.285
2.455PheAsn: 2.455 ± 0.491
1.151PhePro: 1.151 ± 0.283
1.688PheGln: 1.688 ± 0.386
0.921PheArg: 0.921 ± 0.233
2.455PheSer: 2.455 ± 0.446
2.301PheThr: 2.301 ± 0.435
3.068PheVal: 3.068 ± 0.428
0.46PheTrp: 0.46 ± 0.153
1.304PheTyr: 1.304 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
4.449GlyAla: 4.449 ± 0.646
0.384GlyCys: 0.384 ± 0.165
3.912GlyAsp: 3.912 ± 0.517
3.759GlyGlu: 3.759 ± 0.545
2.915GlyPhe: 2.915 ± 0.518
4.296GlyGly: 4.296 ± 0.539
1.458GlyHis: 1.458 ± 0.3
3.759GlyIle: 3.759 ± 0.53
5.293GlyLys: 5.293 ± 0.731
5.063GlyLeu: 5.063 ± 0.76
1.688GlyMet: 1.688 ± 0.4
3.605GlyAsn: 3.605 ± 0.879
0.0GlyPro: 0.0 ± 0.0
2.455GlyGln: 2.455 ± 0.433
2.685GlyArg: 2.685 ± 0.507
6.444GlySer: 6.444 ± 1.181
6.214GlyThr: 6.214 ± 1.072
4.066GlyVal: 4.066 ± 0.668
1.151GlyTrp: 1.151 ± 0.32
3.299GlyTyr: 3.299 ± 0.667
0.0GlyXaa: 0.0 ± 0.0
His
1.764HisAla: 1.764 ± 0.395
0.23HisCys: 0.23 ± 0.148
0.844HisAsp: 0.844 ± 0.208
0.767HisGlu: 0.767 ± 0.294
1.151HisPhe: 1.151 ± 0.367
1.151HisGly: 1.151 ± 0.294
0.307HisHis: 0.307 ± 0.172
1.151HisIle: 1.151 ± 0.291
1.151HisLys: 1.151 ± 0.335
1.688HisLeu: 1.688 ± 0.423
0.614HisMet: 0.614 ± 0.221
0.614HisAsn: 0.614 ± 0.187
0.767HisPro: 0.767 ± 0.193
0.384HisGln: 0.384 ± 0.197
0.537HisArg: 0.537 ± 0.197
0.997HisSer: 0.997 ± 0.363
1.227HisThr: 1.227 ± 0.304
0.997HisVal: 0.997 ± 0.281
0.307HisTrp: 0.307 ± 0.137
0.46HisTyr: 0.46 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
6.06IleAla: 6.06 ± 0.542
0.537IleCys: 0.537 ± 0.22
4.066IleAsp: 4.066 ± 0.469
4.833IleGlu: 4.833 ± 0.734
1.841IlePhe: 1.841 ± 0.534
4.066IleGly: 4.066 ± 0.677
0.921IleHis: 0.921 ± 0.286
4.219IleIle: 4.219 ± 0.574
5.446IleLys: 5.446 ± 0.76
3.759IleLeu: 3.759 ± 0.437
1.918IleMet: 1.918 ± 0.46
4.142IleAsn: 4.142 ± 0.97
2.762IlePro: 2.762 ± 0.426
1.841IleGln: 1.841 ± 0.423
2.148IleArg: 2.148 ± 0.417
3.836IleSer: 3.836 ± 0.503
4.756IleThr: 4.756 ± 0.998
3.912IleVal: 3.912 ± 0.466
0.537IleTrp: 0.537 ± 0.207
1.994IleTyr: 1.994 ± 0.433
0.0IleXaa: 0.0 ± 0.0
Lys
5.216LysAla: 5.216 ± 0.94
0.537LysCys: 0.537 ± 0.222
4.909LysAsp: 4.909 ± 0.751
6.06LysGlu: 6.06 ± 0.942
3.605LysPhe: 3.605 ± 0.558
4.756LysGly: 4.756 ± 0.839
1.534LysHis: 1.534 ± 0.309
5.677LysIle: 5.677 ± 0.776
7.441LysLys: 7.441 ± 1.2
6.674LysLeu: 6.674 ± 0.761
2.148LysMet: 2.148 ± 0.409
3.605LysAsn: 3.605 ± 0.479
2.915LysPro: 2.915 ± 0.45
2.838LysGln: 2.838 ± 0.627
4.909LysArg: 4.909 ± 0.839
5.907LysSer: 5.907 ± 1.026
4.986LysThr: 4.986 ± 0.719
4.526LysVal: 4.526 ± 0.667
0.844LysTrp: 0.844 ± 0.266
3.145LysTyr: 3.145 ± 0.578
0.0LysXaa: 0.0 ± 0.0
Leu
6.367LeuAla: 6.367 ± 0.657
0.614LeuCys: 0.614 ± 0.256
5.753LeuAsp: 5.753 ± 0.712
5.216LeuGlu: 5.216 ± 0.809
2.148LeuPhe: 2.148 ± 0.486
3.759LeuGly: 3.759 ± 0.521
1.688LeuHis: 1.688 ± 0.379
3.989LeuIle: 3.989 ± 0.476
7.288LeuLys: 7.288 ± 0.89
6.214LeuLeu: 6.214 ± 0.922
1.918LeuMet: 1.918 ± 0.413
4.986LeuAsn: 4.986 ± 0.5
3.222LeuPro: 3.222 ± 0.357
2.915LeuGln: 2.915 ± 0.514
3.375LeuArg: 3.375 ± 0.558
5.677LeuSer: 5.677 ± 0.481
4.833LeuThr: 4.833 ± 0.697
3.989LeuVal: 3.989 ± 0.523
0.767LeuTrp: 0.767 ± 0.321
2.685LeuTyr: 2.685 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
2.608MetAla: 2.608 ± 0.423
0.23MetCys: 0.23 ± 0.148
1.227MetAsp: 1.227 ± 0.269
1.534MetGlu: 1.534 ± 0.354
0.614MetPhe: 0.614 ± 0.199
2.225MetGly: 2.225 ± 0.454
0.153MetHis: 0.153 ± 0.103
1.611MetIle: 1.611 ± 0.417
2.455MetLys: 2.455 ± 0.581
1.841MetLeu: 1.841 ± 0.519
0.997MetMet: 0.997 ± 0.336
1.304MetAsn: 1.304 ± 0.261
1.611MetPro: 1.611 ± 0.367
0.844MetGln: 0.844 ± 0.263
1.688MetArg: 1.688 ± 0.394
2.531MetSer: 2.531 ± 0.425
1.151MetThr: 1.151 ± 0.255
1.304MetVal: 1.304 ± 0.422
0.537MetTrp: 0.537 ± 0.177
0.69MetTyr: 0.69 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
4.449AsnAla: 4.449 ± 0.811
0.614AsnCys: 0.614 ± 0.233
3.375AsnAsp: 3.375 ± 0.42
2.608AsnGlu: 2.608 ± 0.411
2.685AsnPhe: 2.685 ± 0.458
4.986AsnGly: 4.986 ± 0.743
0.767AsnHis: 0.767 ± 0.208
4.526AsnIle: 4.526 ± 1.202
4.526AsnLys: 4.526 ± 0.639
4.833AsnLeu: 4.833 ± 0.54
1.227AsnMet: 1.227 ± 0.399
4.219AsnAsn: 4.219 ± 0.663
2.378AsnPro: 2.378 ± 0.412
1.764AsnGln: 1.764 ± 0.4
2.225AsnArg: 2.225 ± 0.429
4.986AsnSer: 4.986 ± 1.228
5.6AsnThr: 5.6 ± 1.243
3.145AsnVal: 3.145 ± 0.508
0.384AsnTrp: 0.384 ± 0.171
2.225AsnTyr: 2.225 ± 0.495
0.0AsnXaa: 0.0 ± 0.0
Pro
1.764ProAla: 1.764 ± 0.328
0.23ProCys: 0.23 ± 0.127
1.458ProAsp: 1.458 ± 0.32
2.915ProGlu: 2.915 ± 0.694
1.918ProPhe: 1.918 ± 0.329
0.0ProGly: 0.0 ± 0.0
0.537ProHis: 0.537 ± 0.2
1.688ProIle: 1.688 ± 0.35
2.455ProLys: 2.455 ± 0.544
2.455ProLeu: 2.455 ± 0.38
0.844ProMet: 0.844 ± 0.262
1.918ProAsn: 1.918 ± 0.391
0.767ProPro: 0.767 ± 0.22
1.458ProGln: 1.458 ± 0.359
0.614ProArg: 0.614 ± 0.199
2.608ProSer: 2.608 ± 0.34
3.068ProThr: 3.068 ± 0.63
1.534ProVal: 1.534 ± 0.335
0.077ProTrp: 0.077 ± 0.078
1.074ProTyr: 1.074 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
2.762GlnAla: 2.762 ± 0.511
0.153GlnCys: 0.153 ± 0.11
2.378GlnAsp: 2.378 ± 0.422
2.992GlnGlu: 2.992 ± 0.708
1.227GlnPhe: 1.227 ± 0.327
1.534GlnGly: 1.534 ± 0.382
1.151GlnHis: 1.151 ± 0.272
2.531GlnIle: 2.531 ± 0.49
3.299GlnLys: 3.299 ± 0.577
2.762GlnLeu: 2.762 ± 0.539
1.304GlnMet: 1.304 ± 0.495
1.534GlnAsn: 1.534 ± 0.403
0.46GlnPro: 0.46 ± 0.18
1.611GlnGln: 1.611 ± 0.454
1.611GlnArg: 1.611 ± 0.306
2.762GlnSer: 2.762 ± 0.438
2.915GlnThr: 2.915 ± 0.517
1.918GlnVal: 1.918 ± 0.443
0.614GlnTrp: 0.614 ± 0.164
1.304GlnTyr: 1.304 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
3.068ArgAla: 3.068 ± 0.544
0.0ArgCys: 0.0 ± 0.0
2.608ArgAsp: 2.608 ± 0.469
2.685ArgGlu: 2.685 ± 0.472
1.227ArgPhe: 1.227 ± 0.266
2.531ArgGly: 2.531 ± 0.406
0.997ArgHis: 0.997 ± 0.313
1.918ArgIle: 1.918 ± 0.314
3.912ArgLys: 3.912 ± 0.747
4.142ArgLeu: 4.142 ± 0.869
1.841ArgMet: 1.841 ± 0.484
1.534ArgAsn: 1.534 ± 0.361
1.074ArgPro: 1.074 ± 0.281
1.534ArgGln: 1.534 ± 0.357
1.994ArgArg: 1.994 ± 0.424
2.378ArgSer: 2.378 ± 0.392
1.994ArgThr: 1.994 ± 0.431
1.918ArgVal: 1.918 ± 0.467
0.614ArgTrp: 0.614 ± 0.197
1.841ArgTyr: 1.841 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
6.137SerAla: 6.137 ± 0.896
0.537SerCys: 0.537 ± 0.195
4.142SerAsp: 4.142 ± 0.525
3.068SerGlu: 3.068 ± 0.586
3.145SerPhe: 3.145 ± 0.502
7.057SerGly: 7.057 ± 1.191
1.458SerHis: 1.458 ± 0.354
5.14SerIle: 5.14 ± 0.7
5.37SerLys: 5.37 ± 0.601
5.523SerLeu: 5.523 ± 0.597
1.688SerMet: 1.688 ± 0.353
4.986SerAsn: 4.986 ± 1.081
1.381SerPro: 1.381 ± 0.316
3.529SerGln: 3.529 ± 0.512
1.764SerArg: 1.764 ± 0.367
5.14SerSer: 5.14 ± 1.255
4.909SerThr: 4.909 ± 0.988
4.679SerVal: 4.679 ± 0.932
1.074SerTrp: 1.074 ± 0.25
2.762SerTyr: 2.762 ± 0.565
0.0SerXaa: 0.0 ± 0.0
Thr
7.901ThrAla: 7.901 ± 1.478
0.46ThrCys: 0.46 ± 0.218
4.909ThrAsp: 4.909 ± 0.708
4.603ThrGlu: 4.603 ± 0.571
2.685ThrPhe: 2.685 ± 0.541
5.83ThrGly: 5.83 ± 0.754
1.074ThrHis: 1.074 ± 0.21
4.679ThrIle: 4.679 ± 0.536
4.603ThrLys: 4.603 ± 0.621
4.833ThrLeu: 4.833 ± 0.772
1.227ThrMet: 1.227 ± 0.266
5.6ThrAsn: 5.6 ± 1.165
2.071ThrPro: 2.071 ± 0.41
2.378ThrGln: 2.378 ± 0.554
1.994ThrArg: 1.994 ± 0.359
6.214ThrSer: 6.214 ± 1.407
5.83ThrThr: 5.83 ± 1.301
4.219ThrVal: 4.219 ± 1.167
0.537ThrTrp: 0.537 ± 0.197
2.071ThrTyr: 2.071 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
5.83ValAla: 5.83 ± 1.259
0.614ValCys: 0.614 ± 0.26
3.299ValAsp: 3.299 ± 0.488
3.759ValGlu: 3.759 ± 0.575
1.994ValPhe: 1.994 ± 0.408
4.142ValGly: 4.142 ± 0.517
1.304ValHis: 1.304 ± 0.42
3.452ValIle: 3.452 ± 0.535
3.912ValLys: 3.912 ± 0.598
3.299ValLeu: 3.299 ± 0.54
0.921ValMet: 0.921 ± 0.301
4.603ValAsn: 4.603 ± 0.923
1.918ValPro: 1.918 ± 0.415
1.841ValGln: 1.841 ± 0.456
2.148ValArg: 2.148 ± 0.389
4.986ValSer: 4.986 ± 0.642
4.449ValThr: 4.449 ± 0.533
2.992ValVal: 2.992 ± 0.384
0.614ValTrp: 0.614 ± 0.245
1.304ValTyr: 1.304 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
0.844TrpAla: 0.844 ± 0.299
0.077TrpCys: 0.077 ± 0.076
0.69TrpAsp: 0.69 ± 0.164
0.537TrpGlu: 0.537 ± 0.219
0.307TrpPhe: 0.307 ± 0.164
0.46TrpGly: 0.46 ± 0.169
0.0TrpHis: 0.0 ± 0.0
0.614TrpIle: 0.614 ± 0.224
1.151TrpLys: 1.151 ± 0.275
1.611TrpLeu: 1.611 ± 0.362
0.0TrpMet: 0.0 ± 0.0
0.921TrpAsn: 0.921 ± 0.237
0.0TrpPro: 0.0 ± 0.0
0.614TrpGln: 0.614 ± 0.243
0.384TrpArg: 0.384 ± 0.16
1.074TrpSer: 1.074 ± 0.222
1.074TrpThr: 1.074 ± 0.289
0.921TrpVal: 0.921 ± 0.254
0.077TrpTrp: 0.077 ± 0.087
0.23TrpTyr: 0.23 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.994TyrAla: 1.994 ± 0.351
0.614TyrCys: 0.614 ± 0.235
1.764TyrAsp: 1.764 ± 0.228
1.304TyrGlu: 1.304 ± 0.362
1.611TyrPhe: 1.611 ± 0.395
2.608TyrGly: 2.608 ± 0.528
0.844TyrHis: 0.844 ± 0.193
1.994TyrIle: 1.994 ± 0.455
3.068TyrLys: 3.068 ± 0.539
3.068TyrLeu: 3.068 ± 0.506
0.537TyrMet: 0.537 ± 0.181
2.301TyrAsn: 2.301 ± 0.371
0.537TyrPro: 0.537 ± 0.212
1.841TyrGln: 1.841 ± 0.397
0.997TyrArg: 0.997 ± 0.283
2.915TyrSer: 2.915 ± 0.418
2.608TyrThr: 2.608 ± 0.44
2.455TyrVal: 2.455 ± 0.441
0.614TyrTrp: 0.614 ± 0.227
1.074TyrTyr: 1.074 ± 0.236
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13037 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski