Acidipropionibacterium acidipropionici (strain ATCC 4875 / DSM 20272 / JCM 6432 / NBRC 12425 / NCIMB 8070) (Propionibacterium acidipropionici)
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3316 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7RNH3|K7RNH3_ACIA4 Uncharacterized protein OS=Acidipropionibacterium acidipropionici (strain ATCC 4875 / DSM 20272 / JCM 6432 / NBRC 12425 / NCIMB 8070) OX=1171373 GN=PACID_17340 PE=4 SV=1
MM1 pKa = 7.72 NDD3 pKa = 3.62 QIPPGILAQFDD14 pKa = 4.12 QIAADD19 pKa = 3.86 HH20 pKa = 6.34 QSMEE24 pKa = 4.86 LLTGMCQAAPEE35 pKa = 4.19 VAAQEE40 pKa = 4.09 IAGFATLLTILSATGSWDD58 pKa = 3.26 GLEE61 pKa = 4.58 DD62 pKa = 4.31 LLVNPPSPLTHH73 pKa = 6.8 PAFAAGCHH81 pKa = 5.64 LAAEE85 pKa = 4.55 SFLTYY90 pKa = 10.17 TDD92 pKa = 3.32 NDD94 pKa = 3.78 KK95 pKa = 11.25 EE96 pKa = 4.16 II97 pKa = 4.88
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.846
IPC_protein 3.745
Toseland 3.567
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|K7S718|K7S718_ACIA4 30S ribosomal protein S7 OS=Acidipropionibacterium acidipropionici (strain ATCC 4875 / DSM 20272 / JCM 6432 / NBRC 12425 / NCIMB 8070) OX=1171373 GN=rpsG PE=3 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 VLGSGVPSARR13 pKa = 11.84 LLTAHH18 pKa = 7.67 RR19 pKa = 11.84 STIPRR24 pKa = 11.84 RR25 pKa = 11.84 PGGPQPTPSTARR37 pKa = 11.84 IGISLRR43 pKa = 11.84 AEE45 pKa = 3.57 PRR47 pKa = 11.84 ARR49 pKa = 11.84 TGGARR54 pKa = 11.84 SAA56 pKa = 3.88
Molecular weight: 5.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.384
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.857
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3316
0
3316
1065160
37
2257
321.2
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.308 ± 0.055
0.84 ± 0.014
6.336 ± 0.04
5.34 ± 0.039
2.799 ± 0.028
9.186 ± 0.039
2.212 ± 0.02
4.488 ± 0.03
2.33 ± 0.037
9.647 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.02
2.055 ± 0.024
5.539 ± 0.029
2.971 ± 0.027
7.444 ± 0.048
6.01 ± 0.038
6.092 ± 0.031
8.54 ± 0.037
1.628 ± 0.021
1.946 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here