Hubei sobemo-like virus 34

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEP2|A0A1L3KEP2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 34 OX=1923221 PE=4 SV=1
MM1 pKa = 7.93PGTSNVEE8 pKa = 4.21SIMDD12 pKa = 3.56IVILKK17 pKa = 9.71QLVIIGKK24 pKa = 8.9CLLVVGIAAGTVFMSVGLYY43 pKa = 9.7TLFSLIPGTIKK54 pKa = 10.82NMLVNWPRR62 pKa = 11.84EE63 pKa = 4.06MVQAKK68 pKa = 9.18YY69 pKa = 10.29RR70 pKa = 11.84EE71 pKa = 4.02YY72 pKa = 10.8LEE74 pKa = 4.9PYY76 pKa = 9.96GLVEE80 pKa = 4.58EE81 pKa = 4.8KK82 pKa = 10.32PEE84 pKa = 3.89PVIPKK89 pKa = 8.98PIKK92 pKa = 9.09IEE94 pKa = 3.77PVRR97 pKa = 11.84VEE99 pKa = 4.34IEE101 pKa = 4.21SLLQRR106 pKa = 11.84CYY108 pKa = 10.73IEE110 pKa = 4.3VCEE113 pKa = 4.9SIYY116 pKa = 11.39LMIGWIFINKK126 pKa = 6.46YY127 pKa = 7.19TVILMCILSLIILVIYY143 pKa = 10.2RR144 pKa = 11.84RR145 pKa = 11.84YY146 pKa = 9.58FRR148 pKa = 11.84HH149 pKa = 6.0FVSKK153 pKa = 10.52YY154 pKa = 8.21YY155 pKa = 10.84KK156 pKa = 9.22KK157 pKa = 8.89TVWWMRR163 pKa = 11.84GVDD166 pKa = 3.95TVTPPYY172 pKa = 9.52RR173 pKa = 11.84VEE175 pKa = 4.32SIIPGSDD182 pKa = 3.48FNPDD186 pKa = 3.18MVIPSYY192 pKa = 10.03QVEE195 pKa = 4.2IHH197 pKa = 6.97SSGIWTDD204 pKa = 2.83KK205 pKa = 10.08FVAYY209 pKa = 9.68GVRR212 pKa = 11.84FHH214 pKa = 7.55DD215 pKa = 4.08FLVTPRR221 pKa = 11.84HH222 pKa = 5.49VLTAVSNLKK231 pKa = 10.54GEE233 pKa = 4.41LMIKK237 pKa = 7.53TRR239 pKa = 11.84RR240 pKa = 11.84GSKK243 pKa = 9.94IIRR246 pKa = 11.84SQIMDD251 pKa = 4.15SIGLNDD257 pKa = 3.37MAYY260 pKa = 9.55IKK262 pKa = 10.7LEE264 pKa = 3.95SSIWADD270 pKa = 3.5LGVTSPSFPKK280 pKa = 10.44QLTEE284 pKa = 4.15VPSSVVTCVGKK295 pKa = 9.74PGGSTGSLKK304 pKa = 9.93FTDD307 pKa = 6.65FMGFWNYY314 pKa = 9.53TGSTVPGMSGAAYY327 pKa = 9.87FIGNVCYY334 pKa = 9.95GVHH337 pKa = 5.87QGVIGNINAGAMSLAIVEE355 pKa = 4.33EE356 pKa = 4.41LKK358 pKa = 11.03VLYY361 pKa = 10.62NPEE364 pKa = 4.37GYY366 pKa = 10.81KK367 pKa = 9.28MPRR370 pKa = 11.84SSTTPLNSDD379 pKa = 2.95AKK381 pKa = 8.47EE382 pKa = 4.47TEE384 pKa = 3.96FEE386 pKa = 4.62TPFTRR391 pKa = 11.84PKK393 pKa = 10.6KK394 pKa = 8.72YY395 pKa = 9.13TKK397 pKa = 10.05EE398 pKa = 3.97DD399 pKa = 3.35YY400 pKa = 11.56AEE402 pKa = 4.52FIKK405 pKa = 10.62KK406 pKa = 9.33KK407 pKa = 9.08QAEE410 pKa = 4.16GDD412 pKa = 3.2LWADD416 pKa = 3.8DD417 pKa = 3.91KK418 pKa = 11.95PEE420 pKa = 4.11FEE422 pKa = 4.74SNRR425 pKa = 11.84KK426 pKa = 9.69GEE428 pKa = 4.78AKK430 pKa = 10.34PEE432 pKa = 3.94MTQEE436 pKa = 3.82EE437 pKa = 5.66LFFKK441 pKa = 10.65ILGLTPEE448 pKa = 4.34QARR451 pKa = 11.84KK452 pKa = 9.39LLPFIDD458 pKa = 3.71QLARR462 pKa = 11.84TPTSTQQPPSEE473 pKa = 4.17EE474 pKa = 4.21KK475 pKa = 8.27MTGHH479 pKa = 6.35SPDD482 pKa = 3.73GPQIPVTKK490 pKa = 9.41IDD492 pKa = 3.32LEE494 pKa = 4.44TRR496 pKa = 11.84VSILEE501 pKa = 3.86NSKK504 pKa = 10.07VRR506 pKa = 11.84LEE508 pKa = 4.5AEE510 pKa = 3.76NSVLKK515 pKa = 10.77EE516 pKa = 4.02KK517 pKa = 10.8VKK519 pKa = 10.69HH520 pKa = 6.28LEE522 pKa = 3.81DD523 pKa = 5.58HH524 pKa = 6.6IDD526 pKa = 4.29KK527 pKa = 10.37IARR530 pKa = 11.84WSVLHH535 pKa = 6.05YY536 pKa = 10.72GFGRR540 pKa = 11.84KK541 pKa = 9.13KK542 pKa = 10.34YY543 pKa = 9.33EE544 pKa = 4.17CAFPQCDD551 pKa = 3.39RR552 pKa = 11.84IFVGKK557 pKa = 9.52QALIAHH563 pKa = 7.3KK564 pKa = 10.11LASDD568 pKa = 3.71HH569 pKa = 6.8LLEE572 pKa = 5.22GDD574 pKa = 3.85KK575 pKa = 10.96LLPNPVVQGEE585 pKa = 4.63SAFPADD591 pKa = 3.52NKK593 pKa = 10.17TKK595 pKa = 10.92EE596 pKa = 3.93EE597 pKa = 4.41LGFQKK602 pKa = 10.7KK603 pKa = 9.12PLQKK607 pKa = 10.2NHH609 pKa = 5.71QPKK612 pKa = 10.31QSGTPLRR619 pKa = 11.84NTLNMPEE626 pKa = 4.21KK627 pKa = 9.6PKK629 pKa = 10.58PSTSTQEE636 pKa = 4.17DD637 pKa = 4.2PNDD640 pKa = 3.85LKK642 pKa = 10.68TMMKK646 pKa = 9.85EE647 pKa = 3.46MHH649 pKa = 7.22KK650 pKa = 10.1ILQILVQKK658 pKa = 10.82SVGPNSATTQNN669 pKa = 3.34

Molecular weight: 75.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEP2|A0A1L3KEP2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 34 OX=1923221 PE=4 SV=1
MM1 pKa = 7.73SYY3 pKa = 9.39NHH5 pKa = 5.82YY6 pKa = 10.31VRR8 pKa = 11.84VVSEE12 pKa = 4.63LEE14 pKa = 4.29WNSSPGVPYY23 pKa = 10.48CHH25 pKa = 6.44QFTTNRR31 pKa = 11.84ILFGVKK37 pKa = 9.84DD38 pKa = 3.94GQPSQEE44 pKa = 3.89RR45 pKa = 11.84LKK47 pKa = 10.13MVWEE51 pKa = 4.33IVSKK55 pKa = 10.75RR56 pKa = 11.84LQDD59 pKa = 3.45QDD61 pKa = 3.42SDD63 pKa = 4.56PIRR66 pKa = 11.84LFVKK70 pKa = 10.11PEE72 pKa = 3.61PHH74 pKa = 6.73KK75 pKa = 10.73KK76 pKa = 10.46SKK78 pKa = 10.16IDD80 pKa = 3.33NKK82 pKa = 10.49AYY84 pKa = 10.71RR85 pKa = 11.84LISSVSVVDD94 pKa = 4.52QIIDD98 pKa = 3.33HH99 pKa = 6.28MLFDD103 pKa = 4.64HH104 pKa = 6.41MNKK107 pKa = 9.86KK108 pKa = 10.36ALDD111 pKa = 3.69NWWNNPVKK119 pKa = 10.72VGWSPVVGGWKK130 pKa = 10.19FIPTNYY136 pKa = 9.73ISTDD140 pKa = 3.0KK141 pKa = 11.3SSWDD145 pKa = 3.15WTVGMWLIEE154 pKa = 4.02LVFEE158 pKa = 4.79LRR160 pKa = 11.84KK161 pKa = 10.17DD162 pKa = 3.44LCVTKK167 pKa = 10.85GPLYY171 pKa = 10.64DD172 pKa = 2.89KK173 pKa = 10.1WLRR176 pKa = 11.84LAIFRR181 pKa = 11.84FTKK184 pKa = 10.47LFYY187 pKa = 10.74DD188 pKa = 4.46FQLVTSGGYY197 pKa = 8.33KK198 pKa = 10.03LRR200 pKa = 11.84YY201 pKa = 8.33KK202 pKa = 10.73GHH204 pKa = 7.19GIMKK208 pKa = 9.41SGCVNTLVDD217 pKa = 3.81NSLMQLLLHH226 pKa = 6.11VMVSFRR232 pKa = 11.84LNKK235 pKa = 10.37SPVDD239 pKa = 3.38IMAMGDD245 pKa = 3.62DD246 pKa = 4.36VIMEE250 pKa = 4.97FIDD253 pKa = 3.69YY254 pKa = 10.68LEE256 pKa = 4.87EE257 pKa = 3.94YY258 pKa = 10.41LAEE261 pKa = 4.29TKK263 pKa = 10.0KK264 pKa = 10.13HH265 pKa = 6.04CIIKK269 pKa = 9.87EE270 pKa = 4.16VNFQTEE276 pKa = 4.35FAGLRR281 pKa = 11.84YY282 pKa = 9.22KK283 pKa = 10.85KK284 pKa = 10.5NGIIEE289 pKa = 3.93PLYY292 pKa = 9.07TGKK295 pKa = 9.7HH296 pKa = 5.56AYY298 pKa = 9.29QLLHH302 pKa = 6.42MPNKK306 pKa = 9.76FKK308 pKa = 11.56NEE310 pKa = 3.37IAASYY315 pKa = 10.14SLLYY319 pKa = 10.31HH320 pKa = 7.16RR321 pKa = 11.84STHH324 pKa = 6.64RR325 pKa = 11.84DD326 pKa = 2.94WIRR329 pKa = 11.84QKK331 pKa = 11.08LEE333 pKa = 4.29SIGCNLPPLYY343 pKa = 10.41EE344 pKa = 5.33LDD346 pKa = 4.62AIFDD350 pKa = 4.3GEE352 pKa = 4.43CC353 pKa = 3.3

Molecular weight: 41.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1022

353

669

511.0

58.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.718 ± 0.466

1.37 ± 0.174

4.697 ± 0.808

6.556 ± 0.767

4.11 ± 0.223

6.067 ± 0.36

2.446 ± 0.502

7.143 ± 0.33

8.513 ± 0.007

9.002 ± 0.63

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.327 ± 0.038

4.012 ± 0.423

6.067 ± 0.957

3.523 ± 0.214

3.914 ± 0.177

6.849 ± 0.027

5.284 ± 0.843

7.436 ± 0.037

1.957 ± 0.61

4.012 ± 0.423
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski