Tenacibaculum jejuense
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3944 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238UEW4|A0A238UEW4_9FLAO Glycosyl transferase group 1 family protein OS=Tenacibaculum jejuense OX=584609 GN=TJEJU_4079 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 KK3 pKa = 8.09 TITFLYY9 pKa = 10.15 AVLISISIFGQTSLSTGDD27 pKa = 3.23 IAFLSVNFDD36 pKa = 3.21 GSDD39 pKa = 3.39 DD40 pKa = 3.62 YY41 pKa = 12.21 SFILFKK47 pKa = 10.97 DD48 pKa = 3.03 IDD50 pKa = 4.47 ANTTITFTDD59 pKa = 4.33 CGWSDD64 pKa = 3.32 GSASFICNVGDD75 pKa = 3.82 ANGWTWLSGSALAAGTVITITNEE98 pKa = 3.35 SSASIGTVSGVTSLLSSAGDD118 pKa = 3.3 QIFAYY123 pKa = 9.76 QGSASSPTFLAGINSNEE140 pKa = 4.21 VADD143 pKa = 4.19 DD144 pKa = 4.18 SNWNGEE150 pKa = 4.17 VTSNSTSALPDD161 pKa = 3.18 QLTNGTNAIRR171 pKa = 11.84 LHH173 pKa = 6.03 NMGSEE178 pKa = 3.99 VDD180 pKa = 3.12 NWQYY184 pKa = 11.46 NCTVVSGDD192 pKa = 3.37 VTTVRR197 pKa = 11.84 AAINNIANWSNNNGTAFSPSAPNCLWNITFASPPSTVTSVTVPSNATYY245 pKa = 10.77 SAGQNLDD252 pKa = 3.34 FTVSFNEE259 pKa = 4.52 TITVNTTGGTPQLALTIGSTTRR281 pKa = 11.84 QATFVSGSGTANLLFRR297 pKa = 11.84 YY298 pKa = 6.91 TVQAGDD304 pKa = 3.78 SDD306 pKa = 4.03 TDD308 pKa = 4.46 GIAVGSLAANGGTIQNGSGTDD329 pKa = 3.33 ADD331 pKa = 4.16 LTLNSIGSTTNVFVDD346 pKa = 5.68 GIAPTGYY353 pKa = 10.25 SVTIDD358 pKa = 3.35 QSPINAANDD367 pKa = 3.7 DD368 pKa = 3.94 AVSFTFASAEE378 pKa = 3.88 VGTTYY383 pKa = 11.09 NYY385 pKa = 11.26 AFTSSGAPGSVTGSGTIATASDD407 pKa = 3.42 QTTGIDD413 pKa = 3.27 ISGLADD419 pKa = 3.41 GMITLSVTLTDD430 pKa = 3.62 VNGNTGAAATDD441 pKa = 3.85 TEE443 pKa = 4.68 TKK445 pKa = 9.33 EE446 pKa = 4.32 TVAPTGYY453 pKa = 10.31 SVTIDD458 pKa = 3.35 QSPINAANDD467 pKa = 3.7 DD468 pKa = 3.94 AVSFTFASAEE478 pKa = 3.88 VGTTYY483 pKa = 11.09 NYY485 pKa = 11.26 AFTSSGAPGSVTGSGTIATASDD507 pKa = 3.98 QITGIDD513 pKa = 3.26 ISGLADD519 pKa = 3.31 GTITLTVTLTDD530 pKa = 3.49 VNGNTGAVATDD541 pKa = 3.69 TEE543 pKa = 4.69 TKK545 pKa = 9.32 EE546 pKa = 4.32 TVAPTGYY553 pKa = 10.31 SVTIDD558 pKa = 3.35 QSPINAANDD567 pKa = 3.66 DD568 pKa = 4.04 VVSFTFASAEE578 pKa = 3.88 VGTTYY583 pKa = 11.09 NYY585 pKa = 11.26 AFTSSGAPGSVTGSGTIATASDD607 pKa = 3.98 QITGIDD613 pKa = 3.26 ISGLADD619 pKa = 3.37 GMITLTVTLTDD630 pKa = 3.49 VNGNTGAAATDD641 pKa = 3.85 TEE643 pKa = 4.68 TKK645 pKa = 9.33 EE646 pKa = 4.32 TVAPTGYY653 pKa = 10.31 SVTIDD658 pKa = 3.35 QSPINAANDD667 pKa = 3.7 DD668 pKa = 3.94 AVSFTFASAEE678 pKa = 3.88 VGTTYY683 pKa = 11.09 NYY685 pKa = 11.26 AFTSSGAPGSVTGSGTIATASDD707 pKa = 3.98 QITGIDD713 pKa = 3.26 ISGLADD719 pKa = 3.31 GTITLTVTLTDD730 pKa = 3.49 VNGNTGAAATDD741 pKa = 3.85 TEE743 pKa = 4.68 TKK745 pKa = 9.33 EE746 pKa = 4.32 TVAPTGYY753 pKa = 10.31 SVTIDD758 pKa = 3.35 QSPINAANDD767 pKa = 3.7 DD768 pKa = 3.94 AVSFTFASAEE778 pKa = 3.88 VGTTYY783 pKa = 11.09 NYY785 pKa = 11.26 AFTSSGAPGSVTGSGIVATATDD807 pKa = 4.36 QITGIDD813 pKa = 3.55 INGLADD819 pKa = 3.5 GTITLTVTLTDD830 pKa = 3.49 VNGNTGAAATDD841 pKa = 4.05 TEE843 pKa = 4.71 TKK845 pKa = 10.29 DD846 pKa = 3.53 ASIPLDD852 pKa = 3.29 PTINVPSGAITVTTTNQTISGNYY875 pKa = 8.5 AEE877 pKa = 4.77 NGITIHH883 pKa = 7.35 AYY885 pKa = 9.64 IDD887 pKa = 3.38 TDD889 pKa = 4.0 NNGTADD895 pKa = 3.58 NTTSLGSAIVTGNAWSFTVNLTADD919 pKa = 3.48 SVNNFVVQAEE929 pKa = 4.26 DD930 pKa = 3.37 ASGNTSTYY938 pKa = 10.46 VDD940 pKa = 4.14 VPTITQTNTITWTGNTSNSWTTAGNWDD967 pKa = 4.22 TNTVPTSAANVIIPSGLTNYY987 pKa = 7.21 PTISSAVTVNSIEE1000 pKa = 4.23 IASGASLIANTSVTANVTYY1019 pKa = 10.64 NRR1021 pKa = 11.84 NLPTTNWYY1029 pKa = 9.84 LVSAPVSNEE1038 pKa = 3.7 TQEE1041 pKa = 5.75 DD1042 pKa = 4.32 IINNPSHH1049 pKa = 6.64 NFATGSGSNIGIGAFTNNGANPWVYY1074 pKa = 9.83 ATNTTTGPLVSGAGVSMKK1092 pKa = 10.32 LAAAGDD1098 pKa = 3.75 VSITGNINTTNVSFPIATGTRR1119 pKa = 11.84 NNFNLIGNPYY1129 pKa = 8.4 TSFVNSSTFAAANTGLLSEE1148 pKa = 4.32 EE1149 pKa = 4.88 TVWLWDD1155 pKa = 3.29 GSQYY1159 pKa = 8.9 VTYY1162 pKa = 11.13 NNMSPIEE1169 pKa = 4.03 VAPAQGFFVEE1179 pKa = 4.86 AAGSGNVTFSTANQSHH1195 pKa = 5.47 QNSDD1199 pKa = 2.98 TFLRR1203 pKa = 11.84 QVPNPTFEE1211 pKa = 5.75 LFIQNDD1217 pKa = 3.17 SDD1219 pKa = 3.66 KK1220 pKa = 11.2 RR1221 pKa = 11.84 STKK1224 pKa = 10.07 VFYY1227 pKa = 11.17 ANGKK1231 pKa = 7.37 TKK1233 pKa = 10.61 GFDD1236 pKa = 2.94 NGYY1239 pKa = 9.25 DD1240 pKa = 3.14 SKK1242 pKa = 10.64 MFAGISEE1249 pKa = 4.17 NFGIFTEE1256 pKa = 4.41 LLEE1259 pKa = 4.39 NNQGKK1264 pKa = 9.57 KK1265 pKa = 10.16 LAIQTLPNSNLEE1277 pKa = 4.17 TMIVPVGIIANAGEE1291 pKa = 4.76 EE1292 pKa = 4.3 ITFSMSSKK1300 pKa = 10.37 NLPTGVEE1307 pKa = 4.1 IYY1309 pKa = 11.08 LEE1311 pKa = 4.3 DD1312 pKa = 3.7 KK1313 pKa = 11.16 VNNTMINLTEE1323 pKa = 4.25 RR1324 pKa = 11.84 NHH1326 pKa = 5.75 TLTLKK1331 pKa = 9.65 TALNASGRR1339 pKa = 11.84 FYY1341 pKa = 10.85 IHH1343 pKa = 5.58 TTAKK1347 pKa = 10.28 RR1348 pKa = 11.84 LSNEE1352 pKa = 4.44 DD1353 pKa = 3.7 IINNLNNVSIYY1364 pKa = 10.61 KK1365 pKa = 9.91 SASKK1369 pKa = 10.41 EE1370 pKa = 3.9 VTISGLQGSGDD1381 pKa = 2.97 IKK1383 pKa = 11.04 VFSILGKK1390 pKa = 9.92 QVGSTSINSNGTSKK1404 pKa = 10.62 IEE1406 pKa = 4.49 LPDD1409 pKa = 4.2 LPSGIYY1415 pKa = 9.59 IVRR1418 pKa = 11.84 LSSEE1422 pKa = 4.1 FGKK1425 pKa = 8.84 LTRR1428 pKa = 11.84 KK1429 pKa = 9.91 IILEE1433 pKa = 3.97
Molecular weight: 147.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A238U9H0|A0A238U9H0_9FLAO Probable ABC-type transport system permease component OS=Tenacibaculum jejuense OX=584609 GN=TJEJU_2160 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.55 FEE4 pKa = 5.36 LYY6 pKa = 10.21 LIKK9 pKa = 10.16 IIMQSYY15 pKa = 7.54 NTSLSLINTIIKK27 pKa = 8.33 TDD29 pKa = 3.08 NRR31 pKa = 11.84 ISVRR35 pKa = 11.84 RR36 pKa = 11.84 FFPRR40 pKa = 11.84 PP41 pKa = 3.17
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.97
IPC_protein 10.701
Toseland 10.716
ProMoST 10.409
Dawson 10.833
Bjellqvist 10.54
Wikipedia 11.038
Rodwell 11.155
Grimsley 10.891
Solomon 10.935
Lehninger 10.906
Nozaki 10.687
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.76
Patrickios 10.979
IPC_peptide 10.935
IPC2_peptide 9.326
IPC2.peptide.svr19 8.377
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3944
0
3944
1390372
23
5673
352.5
39.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.574 ± 0.039
0.742 ± 0.015
5.566 ± 0.038
6.654 ± 0.048
5.396 ± 0.04
6.074 ± 0.055
1.663 ± 0.022
8.209 ± 0.042
8.528 ± 0.087
9.117 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.808 ± 0.018
6.892 ± 0.044
3.148 ± 0.031
3.23 ± 0.022
3.285 ± 0.03
6.797 ± 0.048
6.084 ± 0.071
6.122 ± 0.044
1.013 ± 0.014
4.097 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here