Dorea sp. OM02-2LB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; unclassified Dorea

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A374X7G0|A0A374X7G0_9FIRM Uncharacterized protein OS=Dorea sp. OM02-2LB OX=2292347 GN=DXB23_05150 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.37KK3 pKa = 10.21KK4 pKa = 10.18IAGILALTLAFATLLGGCGSKK25 pKa = 7.83MTADD29 pKa = 3.79DD30 pKa = 4.11AKK32 pKa = 11.05SYY34 pKa = 11.14AQACLDD40 pKa = 3.45ASYY43 pKa = 10.7KK44 pKa = 11.16GEE46 pKa = 3.78FDD48 pKa = 4.0EE49 pKa = 5.82YY50 pKa = 11.22LSQTDD55 pKa = 3.53STKK58 pKa = 11.1EE59 pKa = 3.86EE60 pKa = 3.96AQEE63 pKa = 4.13MYY65 pKa = 11.09DD66 pKa = 3.71NGIDD70 pKa = 3.69LTMEE74 pKa = 4.24ASDD77 pKa = 4.2IEE79 pKa = 4.26NSGVSQEE86 pKa = 4.61LQDD89 pKa = 4.39KK90 pKa = 8.3YY91 pKa = 11.12RR92 pKa = 11.84QMFIDD97 pKa = 4.73LYY99 pKa = 11.37KK100 pKa = 10.89NADD103 pKa = 3.6YY104 pKa = 10.66TLDD107 pKa = 3.53NAKK110 pKa = 10.44EE111 pKa = 4.07DD112 pKa = 4.12GEE114 pKa = 5.1DD115 pKa = 3.62GFTVDD120 pKa = 3.39VTVKK124 pKa = 10.23PFLIFEE130 pKa = 4.48GLDD133 pKa = 3.37DD134 pKa = 4.3EE135 pKa = 5.95LNAKK139 pKa = 10.28LEE141 pKa = 4.45EE142 pKa = 4.12IAANMTEE149 pKa = 4.16VPSDD153 pKa = 3.53EE154 pKa = 5.25EE155 pKa = 4.07INEE158 pKa = 3.98MVYY161 pKa = 10.56QAMYY165 pKa = 10.59DD166 pKa = 3.6IISPKK171 pKa = 9.74LSNMEE176 pKa = 3.92YY177 pKa = 10.38GDD179 pKa = 3.95PVTVTIHH186 pKa = 5.89VVPDD190 pKa = 3.85DD191 pKa = 4.4DD192 pKa = 3.86GVYY195 pKa = 10.05YY196 pKa = 11.09VPDD199 pKa = 4.67DD200 pKa = 4.32DD201 pKa = 5.33YY202 pKa = 11.89EE203 pKa = 4.47AVEE206 pKa = 4.12NALLPEE212 pKa = 4.29

Molecular weight: 23.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A374X6H1|A0A374X6H1_9FIRM YicC family protein OS=Dorea sp. OM02-2LB OX=2292347 GN=DXB23_01095 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.87VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2740

0

2740

878107

27

2877

320.5

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.315 ± 0.043

1.481 ± 0.019

5.469 ± 0.04

8.176 ± 0.06

3.921 ± 0.03

7.226 ± 0.05

1.791 ± 0.019

7.19 ± 0.038

7.083 ± 0.044

9.092 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.103 ± 0.023

4.047 ± 0.03

3.291 ± 0.026

3.496 ± 0.03

4.447 ± 0.034

5.603 ± 0.04

5.407 ± 0.042

6.946 ± 0.042

0.887 ± 0.013

4.029 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski