Amino acid dipepetide frequency for Dorea sp. OM02-2LB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.371AlaAla: 6.371 ± 0.125
1.164AlaCys: 1.164 ± 0.039
4.31AlaAsp: 4.31 ± 0.081
5.654AlaGlu: 5.654 ± 0.087
3.019AlaPhe: 3.019 ± 0.064
6.049AlaGly: 6.049 ± 0.096
1.115AlaHis: 1.115 ± 0.038
5.196AlaIle: 5.196 ± 0.077
5.136AlaLys: 5.136 ± 0.086
6.62AlaLeu: 6.62 ± 0.097
2.346AlaMet: 2.346 ± 0.052
2.526AlaAsn: 2.526 ± 0.064
1.951AlaPro: 1.951 ± 0.046
2.33AlaGln: 2.33 ± 0.055
2.828AlaArg: 2.828 ± 0.064
3.826AlaSer: 3.826 ± 0.075
3.445AlaThr: 3.445 ± 0.084
5.948AlaVal: 5.948 ± 0.089
0.608AlaTrp: 0.608 ± 0.03
2.71AlaTyr: 2.71 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
1.065CysAla: 1.065 ± 0.038
0.255CysCys: 0.255 ± 0.018
0.76CysAsp: 0.76 ± 0.03
1.028CysGlu: 1.028 ± 0.035
0.561CysPhe: 0.561 ± 0.028
1.477CysGly: 1.477 ± 0.043
0.301CysHis: 0.301 ± 0.017
1.043CysIle: 1.043 ± 0.038
0.888CysLys: 0.888 ± 0.036
1.24CysLeu: 1.24 ± 0.04
0.463CysMet: 0.463 ± 0.022
0.528CysAsn: 0.528 ± 0.025
0.675CysPro: 0.675 ± 0.033
0.444CysGln: 0.444 ± 0.025
0.692CysArg: 0.692 ± 0.031
0.875CysSer: 0.875 ± 0.037
0.764CysThr: 0.764 ± 0.027
1.096CysVal: 1.096 ± 0.042
0.105CysTrp: 0.105 ± 0.009
0.55CysTyr: 0.55 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.141AspAla: 4.141 ± 0.083
0.803AspCys: 0.803 ± 0.032
2.561AspAsp: 2.561 ± 0.075
4.361AspGlu: 4.361 ± 0.08
2.376AspPhe: 2.376 ± 0.049
4.358AspGly: 4.358 ± 0.103
0.99AspHis: 0.99 ± 0.036
3.871AspIle: 3.871 ± 0.072
3.174AspLys: 3.174 ± 0.068
5.036AspLeu: 5.036 ± 0.074
1.722AspMet: 1.722 ± 0.042
1.826AspAsn: 1.826 ± 0.054
2.052AspPro: 2.052 ± 0.054
1.838AspGln: 1.838 ± 0.056
2.394AspArg: 2.394 ± 0.056
3.047AspSer: 3.047 ± 0.073
3.018AspThr: 3.018 ± 0.061
3.818AspVal: 3.818 ± 0.075
0.605AspTrp: 0.605 ± 0.026
2.697AspTyr: 2.697 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.758GluAla: 5.758 ± 0.088
0.859GluCys: 0.859 ± 0.031
4.474GluAsp: 4.474 ± 0.077
8.698GluGlu: 8.698 ± 0.164
2.563GluPhe: 2.563 ± 0.049
4.58GluGly: 4.58 ± 0.084
1.484GluHis: 1.484 ± 0.043
6.152GluIle: 6.152 ± 0.105
7.498GluLys: 7.498 ± 0.12
7.285GluLeu: 7.285 ± 0.095
2.664GluMet: 2.664 ± 0.064
4.094GluAsn: 4.094 ± 0.071
1.946GluPro: 1.946 ± 0.051
3.381GluGln: 3.381 ± 0.06
3.73GluArg: 3.73 ± 0.071
3.55GluSer: 3.55 ± 0.072
4.115GluThr: 4.115 ± 0.075
4.973GluVal: 4.973 ± 0.071
0.672GluTrp: 0.672 ± 0.031
3.283GluTyr: 3.283 ± 0.071
0.0GluXaa: 0.0 ± 0.0
Phe
2.862PheAla: 2.862 ± 0.064
0.738PheCys: 0.738 ± 0.029
2.363PheAsp: 2.363 ± 0.057
2.677PheGlu: 2.677 ± 0.056
1.738PhePhe: 1.738 ± 0.059
3.103PheGly: 3.103 ± 0.072
0.868PheHis: 0.868 ± 0.032
2.431PheIle: 2.431 ± 0.057
1.787PheLys: 1.787 ± 0.043
4.074PheLeu: 4.074 ± 0.093
1.102PheMet: 1.102 ± 0.039
1.293PheAsn: 1.293 ± 0.039
1.483PhePro: 1.483 ± 0.041
1.468PheGln: 1.468 ± 0.046
1.75PheArg: 1.75 ± 0.048
2.627PheSer: 2.627 ± 0.064
2.119PheThr: 2.119 ± 0.05
2.758PheVal: 2.758 ± 0.064
0.393PheTrp: 0.393 ± 0.024
1.576PheTyr: 1.576 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.163GlyAla: 5.163 ± 0.095
1.265GlyCys: 1.265 ± 0.045
3.333GlyAsp: 3.333 ± 0.072
5.142GlyGlu: 5.142 ± 0.094
2.994GlyPhe: 2.994 ± 0.06
5.035GlyGly: 5.035 ± 0.103
1.318GlyHis: 1.318 ± 0.046
6.422GlyIle: 6.422 ± 0.083
5.742GlyLys: 5.742 ± 0.09
5.926GlyLeu: 5.926 ± 0.091
2.554GlyMet: 2.554 ± 0.061
3.172GlyAsn: 3.172 ± 0.071
1.281GlyPro: 1.281 ± 0.043
2.196GlyGln: 2.196 ± 0.057
2.905GlyArg: 2.905 ± 0.062
4.105GlySer: 4.105 ± 0.073
4.531GlyThr: 4.531 ± 0.098
5.192GlyVal: 5.192 ± 0.088
0.722GlyTrp: 0.722 ± 0.042
3.258GlyTyr: 3.258 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
1.171HisAla: 1.171 ± 0.04
0.331HisCys: 0.331 ± 0.018
0.873HisAsp: 0.873 ± 0.035
1.069HisGlu: 1.069 ± 0.04
0.891HisPhe: 0.891 ± 0.031
1.277HisGly: 1.277 ± 0.043
0.467HisHis: 0.467 ± 0.033
1.312HisIle: 1.312 ± 0.047
0.979HisLys: 0.979 ± 0.032
1.729HisLeu: 1.729 ± 0.042
0.558HisMet: 0.558 ± 0.025
0.658HisAsn: 0.658 ± 0.029
0.986HisPro: 0.986 ± 0.038
0.679HisGln: 0.679 ± 0.028
0.832HisArg: 0.832 ± 0.033
0.973HisSer: 0.973 ± 0.035
1.023HisThr: 1.023 ± 0.038
1.203HisVal: 1.203 ± 0.034
0.164HisTrp: 0.164 ± 0.015
0.74HisTyr: 0.74 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.553IleAla: 5.553 ± 0.087
1.395IleCys: 1.395 ± 0.051
3.795IleAsp: 3.795 ± 0.071
4.821IleGlu: 4.821 ± 0.084
2.912IlePhe: 2.912 ± 0.068
5.352IleGly: 5.352 ± 0.081
1.425IleHis: 1.425 ± 0.041
4.297IleIle: 4.297 ± 0.091
3.867IleLys: 3.867 ± 0.078
7.521IleLeu: 7.521 ± 0.116
1.836IleMet: 1.836 ± 0.061
2.489IleAsn: 2.489 ± 0.052
3.207IlePro: 3.207 ± 0.071
2.668IleGln: 2.668 ± 0.055
3.931IleArg: 3.931 ± 0.072
4.758IleSer: 4.758 ± 0.077
3.961IleThr: 3.961 ± 0.079
4.772IleVal: 4.772 ± 0.074
0.599IleTrp: 0.599 ± 0.024
2.747IleTyr: 2.747 ± 0.069
0.0IleXaa: 0.0 ± 0.0
Lys
5.053LysAla: 5.053 ± 0.08
0.725LysCys: 0.725 ± 0.031
4.052LysAsp: 4.052 ± 0.075
7.745LysGlu: 7.745 ± 0.108
1.908LysPhe: 1.908 ± 0.054
4.289LysGly: 4.289 ± 0.081
1.117LysHis: 1.117 ± 0.038
5.093LysIle: 5.093 ± 0.088
6.566LysLys: 6.566 ± 0.097
5.377LysLeu: 5.377 ± 0.083
2.434LysMet: 2.434 ± 0.054
3.58LysAsn: 3.58 ± 0.076
1.914LysPro: 1.914 ± 0.044
2.558LysGln: 2.558 ± 0.06
3.467LysArg: 3.467 ± 0.075
3.283LysSer: 3.283 ± 0.066
3.763LysThr: 3.763 ± 0.074
4.586LysVal: 4.586 ± 0.081
0.561LysTrp: 0.561 ± 0.028
2.756LysTyr: 2.756 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.631LeuAla: 6.631 ± 0.098
1.449LeuCys: 1.449 ± 0.047
5.143LeuAsp: 5.143 ± 0.084
7.006LeuGlu: 7.006 ± 0.115
3.685LeuPhe: 3.685 ± 0.08
6.162LeuGly: 6.162 ± 0.102
1.602LeuHis: 1.602 ± 0.046
6.187LeuIle: 6.187 ± 0.097
6.817LeuLys: 6.817 ± 0.103
8.686LeuLeu: 8.686 ± 0.141
2.653LeuMet: 2.653 ± 0.06
3.772LeuAsn: 3.772 ± 0.073
3.535LeuPro: 3.535 ± 0.074
2.972LeuGln: 2.972 ± 0.06
3.736LeuArg: 3.736 ± 0.067
6.097LeuSer: 6.097 ± 0.104
5.124LeuThr: 5.124 ± 0.082
5.671LeuVal: 5.671 ± 0.077
0.707LeuTrp: 0.707 ± 0.034
3.281LeuTyr: 3.281 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.398MetAla: 2.398 ± 0.06
0.342MetCys: 0.342 ± 0.022
1.829MetAsp: 1.829 ± 0.046
2.84MetGlu: 2.84 ± 0.067
1.073MetPhe: 1.073 ± 0.037
2.077MetGly: 2.077 ± 0.06
0.458MetHis: 0.458 ± 0.027
2.261MetIle: 2.261 ± 0.057
2.722MetLys: 2.722 ± 0.056
2.702MetLeu: 2.702 ± 0.056
0.929MetMet: 0.929 ± 0.037
1.463MetAsn: 1.463 ± 0.049
1.053MetPro: 1.053 ± 0.036
1.118MetGln: 1.118 ± 0.036
1.253MetArg: 1.253 ± 0.04
1.758MetSer: 1.758 ± 0.05
1.747MetThr: 1.747 ± 0.044
1.919MetVal: 1.919 ± 0.046
0.23MetTrp: 0.23 ± 0.016
0.86MetTyr: 0.86 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.973AsnAla: 2.973 ± 0.048
0.621AsnCys: 0.621 ± 0.029
1.829AsnAsp: 1.829 ± 0.046
2.691AsnGlu: 2.691 ± 0.054
1.406AsnPhe: 1.406 ± 0.042
3.472AsnGly: 3.472 ± 0.086
0.829AsnHis: 0.829 ± 0.035
2.909AsnIle: 2.909 ± 0.069
2.295AsnLys: 2.295 ± 0.062
3.863AsnLeu: 3.863 ± 0.068
1.274AsnMet: 1.274 ± 0.035
1.542AsnAsn: 1.542 ± 0.056
1.944AsnPro: 1.944 ± 0.052
1.621AsnGln: 1.621 ± 0.045
2.057AsnArg: 2.057 ± 0.054
2.147AsnSer: 2.147 ± 0.062
2.093AsnThr: 2.093 ± 0.055
2.782AsnVal: 2.782 ± 0.062
0.392AsnTrp: 0.392 ± 0.021
1.728AsnTyr: 1.728 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.299ProAla: 2.299 ± 0.057
0.449ProCys: 0.449 ± 0.024
2.215ProAsp: 2.215 ± 0.054
3.525ProGlu: 3.525 ± 0.073
1.472ProPhe: 1.472 ± 0.048
2.345ProGly: 2.345 ± 0.05
0.571ProHis: 0.571 ± 0.026
2.201ProIle: 2.201 ± 0.055
2.13ProLys: 2.13 ± 0.051
2.723ProLeu: 2.723 ± 0.064
0.968ProMet: 0.968 ± 0.036
1.283ProAsn: 1.283 ± 0.041
0.675ProPro: 0.675 ± 0.03
1.002ProGln: 1.002 ± 0.033
1.015ProArg: 1.015 ± 0.034
1.733ProSer: 1.733 ± 0.046
1.689ProThr: 1.689 ± 0.05
2.852ProVal: 2.852 ± 0.058
0.286ProTrp: 0.286 ± 0.02
1.482ProTyr: 1.482 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
2.648GlnAla: 2.648 ± 0.055
0.399GlnCys: 0.399 ± 0.025
1.709GlnAsp: 1.709 ± 0.04
3.106GlnGlu: 3.106 ± 0.065
1.171GlnPhe: 1.171 ± 0.033
2.155GlnGly: 2.155 ± 0.045
0.52GlnHis: 0.52 ± 0.024
2.97GlnIle: 2.97 ± 0.068
3.07GlnLys: 3.07 ± 0.063
2.811GlnLeu: 2.811 ± 0.063
1.275GlnMet: 1.275 ± 0.037
1.705GlnAsn: 1.705 ± 0.058
0.929GlnPro: 0.929 ± 0.031
1.23GlnGln: 1.23 ± 0.04
1.471GlnArg: 1.471 ± 0.038
1.715GlnSer: 1.715 ± 0.043
1.97GlnThr: 1.97 ± 0.049
2.468GlnVal: 2.468 ± 0.058
0.311GlnTrp: 0.311 ± 0.02
1.327GlnTyr: 1.327 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
2.634ArgAla: 2.634 ± 0.056
0.608ArgCys: 0.608 ± 0.028
2.108ArgAsp: 2.108 ± 0.051
4.097ArgGlu: 4.097 ± 0.09
1.821ArgPhe: 1.821 ± 0.045
2.467ArgGly: 2.467 ± 0.055
0.766ArgHis: 0.766 ± 0.031
3.563ArgIle: 3.563 ± 0.067
3.843ArgLys: 3.843 ± 0.075
3.956ArgLeu: 3.956 ± 0.071
1.638ArgMet: 1.638 ± 0.044
2.001ArgAsn: 2.001 ± 0.049
1.379ArgPro: 1.379 ± 0.054
1.81ArgGln: 1.81 ± 0.051
2.24ArgArg: 2.24 ± 0.052
2.232ArgSer: 2.232 ± 0.049
2.291ArgThr: 2.291 ± 0.054
2.721ArgVal: 2.721 ± 0.064
0.38ArgTrp: 0.38 ± 0.022
1.913ArgTyr: 1.913 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
4.088SerAla: 4.088 ± 0.072
0.809SerCys: 0.809 ± 0.036
3.268SerAsp: 3.268 ± 0.082
4.138SerGlu: 4.138 ± 0.071
2.467SerPhe: 2.467 ± 0.056
5.056SerGly: 5.056 ± 0.085
0.999SerHis: 0.999 ± 0.035
3.673SerIle: 3.673 ± 0.075
3.434SerLys: 3.434 ± 0.069
4.878SerLeu: 4.878 ± 0.081
1.797SerMet: 1.797 ± 0.05
2.083SerAsn: 2.083 ± 0.056
1.672SerPro: 1.672 ± 0.042
1.765SerGln: 1.765 ± 0.052
2.617SerArg: 2.617 ± 0.054
3.293SerSer: 3.293 ± 0.091
2.739SerThr: 2.739 ± 0.057
4.314SerVal: 4.314 ± 0.074
0.556SerTrp: 0.556 ± 0.026
2.388SerTyr: 2.388 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
4.202ThrAla: 4.202 ± 0.086
0.667ThrCys: 0.667 ± 0.025
3.219ThrAsp: 3.219 ± 0.069
4.225ThrGlu: 4.225 ± 0.077
2.207ThrPhe: 2.207 ± 0.055
4.788ThrGly: 4.788 ± 0.077
0.863ThrHis: 0.863 ± 0.034
3.919ThrIle: 3.919 ± 0.073
3.182ThrLys: 3.182 ± 0.061
4.919ThrLeu: 4.919 ± 0.083
1.436ThrMet: 1.436 ± 0.04
1.835ThrAsn: 1.835 ± 0.052
2.122ThrPro: 2.122 ± 0.058
1.568ThrGln: 1.568 ± 0.051
2.089ThrArg: 2.089 ± 0.053
2.919ThrSer: 2.919 ± 0.065
2.837ThrThr: 2.837 ± 0.062
4.356ThrVal: 4.356 ± 0.08
0.51ThrTrp: 0.51 ± 0.026
2.207ThrTyr: 2.207 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.905ValAla: 4.905 ± 0.083
1.165ValCys: 1.165 ± 0.04
3.872ValAsp: 3.872 ± 0.068
5.094ValGlu: 5.094 ± 0.082
2.837ValPhe: 2.837 ± 0.059
4.474ValGly: 4.474 ± 0.081
1.178ValHis: 1.178 ± 0.039
5.112ValIle: 5.112 ± 0.086
4.685ValLys: 4.685 ± 0.079
6.818ValLeu: 6.818 ± 0.096
2.003ValMet: 2.003 ± 0.053
2.623ValAsn: 2.623 ± 0.048
2.5ValPro: 2.5 ± 0.056
2.181ValGln: 2.181 ± 0.048
3.017ValArg: 3.017 ± 0.064
4.486ValSer: 4.486 ± 0.082
4.251ValThr: 4.251 ± 0.089
4.923ValVal: 4.923 ± 0.089
0.667ValTrp: 0.667 ± 0.032
2.667ValTyr: 2.667 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.522TrpAla: 0.522 ± 0.029
0.146TrpCys: 0.146 ± 0.013
0.489TrpAsp: 0.489 ± 0.027
0.687TrpGlu: 0.687 ± 0.032
0.389TrpPhe: 0.389 ± 0.025
0.601TrpGly: 0.601 ± 0.029
0.154TrpHis: 0.154 ± 0.014
0.753TrpIle: 0.753 ± 0.036
0.877TrpLys: 0.877 ± 0.033
0.836TrpLeu: 0.836 ± 0.035
0.327TrpMet: 0.327 ± 0.02
0.459TrpAsn: 0.459 ± 0.02
0.177TrpPro: 0.177 ± 0.014
0.353TrpGln: 0.353 ± 0.022
0.346TrpArg: 0.346 ± 0.02
0.426TrpSer: 0.426 ± 0.024
0.397TrpThr: 0.397 ± 0.024
0.507TrpVal: 0.507 ± 0.029
0.088TrpTrp: 0.088 ± 0.01
0.341TrpTyr: 0.341 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.716TyrAla: 2.716 ± 0.052
0.623TyrCys: 0.623 ± 0.029
2.483TyrAsp: 2.483 ± 0.065
3.155TyrGlu: 3.155 ± 0.07
1.721TyrPhe: 1.721 ± 0.047
3.139TyrGly: 3.139 ± 0.057
0.892TyrHis: 0.892 ± 0.038
2.541TyrIle: 2.541 ± 0.054
2.135TyrLys: 2.135 ± 0.055
3.92TyrLeu: 3.92 ± 0.083
1.051TyrMet: 1.051 ± 0.041
1.535TyrAsn: 1.535 ± 0.042
1.433TyrPro: 1.433 ± 0.045
1.778TyrGln: 1.778 ± 0.052
2.1TyrArg: 2.1 ± 0.049
2.182TyrSer: 2.182 ± 0.051
2.192TyrThr: 2.192 ± 0.054
2.6TyrVal: 2.6 ± 0.056
0.317TyrTrp: 0.317 ± 0.019
1.775TyrTyr: 1.775 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2740 proteins (878107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski