Neokomagataea tanensis
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2177 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6V7D6|A0A4Y6V7D6_9PROT Uncharacterized protein OS=Neokomagataea tanensis OX=661191 GN=D5366_01990 PE=4 SV=1
MM1 pKa = 7.51 AVVTVHH7 pKa = 5.76 GTTARR12 pKa = 11.84 TLAVTVDD19 pKa = 3.77 GARR22 pKa = 11.84 AQALANQYY30 pKa = 11.29 ASGINSQFGANSSSVIDD47 pKa = 3.98 LVAGTVSSGSYY58 pKa = 9.13 GHH60 pKa = 6.64 GAISEE65 pKa = 4.19 GGNYY69 pKa = 10.13 SIGGSLSNITVGAYY83 pKa = 10.11 DD84 pKa = 4.57 VVDD87 pKa = 3.76 TLNAAVSIDD96 pKa = 3.87 LTQSTASGVSIIAGDD111 pKa = 3.57 WAGVTVNPGSQSGIFVGGVGNNLFAGSQASGNWNIATGSGNDD153 pKa = 4.26 TINATNGNNTIAGGTGYY170 pKa = 11.29 NLITVGSGTNSVLSEE185 pKa = 4.17 GQDD188 pKa = 3.69 TIRR191 pKa = 11.84 GTSGTNTVTLLGGSSLVSLGANSTIIDD218 pKa = 3.96 GAQDD222 pKa = 3.52 NNVSFGTNSYY232 pKa = 8.09 VTAGSASSYY241 pKa = 10.42 FSTGSISTVVGGLNDD256 pKa = 4.29 TISATGNLQVVRR268 pKa = 11.84 GSTNNISVSGALSFLNGTGSTTITAGTSTIFGATALNAQLVGSGTSLFVGNDD320 pKa = 3.12 GGEE323 pKa = 4.44 TVSGASYY330 pKa = 10.02 TGVLHH335 pKa = 7.16 AFAGTGNDD343 pKa = 4.06 SITGGTGADD352 pKa = 3.45 TLVGGTGNATLTGGTGAANLFALNKK377 pKa = 10.02 GSAGGNYY384 pKa = 9.21 TIEE387 pKa = 4.75 DD388 pKa = 3.89 FGSAAGNLMALYY400 pKa = 10.25 QYY402 pKa = 11.28 GLQNNNGLQSVLQNATVAGGNTTIQLSDD430 pKa = 3.07 NSKK433 pKa = 8.04 ITFVGVTNLKK443 pKa = 10.43 ASNFTLSS450 pKa = 3.04
Molecular weight: 43.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 3.986
IPC_protein 3.973
Toseland 3.745
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.961
Rodwell 3.795
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.821
EMBOSS 3.961
Sillero 4.101
Patrickios 0.566
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A4Y6VBQ6|A0A4Y6VBQ6_9PROT Aldose 1-epimerase OS=Neokomagataea tanensis OX=661191 GN=D5366_06360 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.51 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.83 ATVGGRR28 pKa = 11.84 NILANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.87 GRR39 pKa = 11.84 AKK41 pKa = 10.73 LSAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2177
0
2177
737012
26
1510
338.5
36.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.939 ± 0.068
0.972 ± 0.015
5.294 ± 0.035
5.357 ± 0.053
3.687 ± 0.033
8.083 ± 0.052
2.513 ± 0.028
5.38 ± 0.039
3.487 ± 0.038
10.318 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.023
3.359 ± 0.042
5.135 ± 0.038
3.752 ± 0.034
6.486 ± 0.052
6.373 ± 0.045
5.808 ± 0.044
7.02 ± 0.046
1.315 ± 0.019
2.409 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here